Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis
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2015
Authors
Petković, F.Zivanović, J.
Blazevski, J.
Timotijević, Gordana
Momcilović, M.
Stanojević, Z.
Stamenković, V.
Milosević, V.
Mostarica-Stojković, Marija
Miljković, D.
Article (Published version)
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Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher ...in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.
Keywords:
rat / neuroinflammation / matrix metalloproteinase / gelatinase / experimental autoimmune encephalomyelitisSource:
Neuroscience, 2015, 292, 1-12Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
- Immunopathogenic and regulatory mechanisms in autoimmune diseases and chronic inflamation (RS-MESTD-Basic Research (BR or ON)-175038)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.neuroscience.2015.02.015
ISSN: 0306-4522
PubMed: 25701126
WoS: 000351664700001
Scopus: 2-s2.0-84928548363
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Petković, F. AU - Zivanović, J. AU - Blazevski, J. AU - Timotijević, Gordana AU - Momcilović, M. AU - Stanojević, Z. AU - Stamenković, V. AU - Milosević, V. AU - Mostarica-Stojković, Marija AU - Miljković, D. PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/844 AB - Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Neuroscience T1 - Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis EP - 12 SP - 1 VL - 292 DO - 10.1016/j.neuroscience.2015.02.015 ER -
@article{ author = "Petković, F. and Zivanović, J. and Blazevski, J. and Timotijević, Gordana and Momcilović, M. and Stanojević, Z. and Stamenković, V. and Milosević, V. and Mostarica-Stojković, Marija and Miljković, D.", year = "2015", abstract = "Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Neuroscience", title = "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis", pages = "12-1", volume = "292", doi = "10.1016/j.neuroscience.2015.02.015" }
Petković, F., Zivanović, J., Blazevski, J., Timotijević, G., Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, M.,& Miljković, D.. (2015). Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience Pergamon-Elsevier Science Ltd, Oxford., 292, 1-12. https://doi.org/10.1016/j.neuroscience.2015.02.015
Petković F, Zivanović J, Blazevski J, Timotijević G, Momcilović M, Stanojević Z, Stamenković V, Milosević V, Mostarica-Stojković M, Miljković D. Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience. 2015;292:1-12. doi:10.1016/j.neuroscience.2015.02.015 .
Petković, F., Zivanović, J., Blazevski, J., Timotijević, Gordana, Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, Marija, Miljković, D., "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis" in Neuroscience, 292 (2015):1-12, https://doi.org/10.1016/j.neuroscience.2015.02.015 . .