Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat
Samo za registrovane korisnike
2015
Autori
Tesić, VesnaPerović, Milka
Lazić, Divna
Kojić, Snežana
Smiljanić, Kosara
Ruzdijić, Sabera
Rakić, Ljubisav
Kanazir, Selma
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results r...eveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.
Ključne reči:
Intermittent feeding / Hippocampus / Glucocorticoid receptor isoforms / Dietary restriction / Corticosterone / AgingIzvor:
Journal of Steroid Biochemistry and Molecular Biology, 2015, 149, 43-52Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Plastičnost mozga tokom starenja: uticaj dijetalne restrikcije i anestezije (RS-MESTD-Basic Research (BR or ON)-173056)
- NIH [R03AG046216]
- NATIONAL INSTITUTE ON AGING [R03AG046216] Funding Source: NIH RePORTER
DOI: 10.1016/j.jsbmb.2015.01.013
ISSN: 0960-0760
PubMed: 25616002
WoS: 000352171400006
Scopus: 2-s2.0-84921839259
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Tesić, Vesna AU - Perović, Milka AU - Lazić, Divna AU - Kojić, Snežana AU - Smiljanić, Kosara AU - Ruzdijić, Sabera AU - Rakić, Ljubisav AU - Kanazir, Selma PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/880 AB - Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Steroid Biochemistry and Molecular Biology T1 - Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat EP - 52 SP - 43 VL - 149 DO - 10.1016/j.jsbmb.2015.01.013 ER -
@article{ author = "Tesić, Vesna and Perović, Milka and Lazić, Divna and Kojić, Snežana and Smiljanić, Kosara and Ruzdijić, Sabera and Rakić, Ljubisav and Kanazir, Selma", year = "2015", abstract = "Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Steroid Biochemistry and Molecular Biology", title = "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat", pages = "52-43", volume = "149", doi = "10.1016/j.jsbmb.2015.01.013" }
Tesić, V., Perović, M., Lazić, D., Kojić, S., Smiljanić, K., Ruzdijić, S., Rakić, L.,& Kanazir, S.. (2015). Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology Pergamon-Elsevier Science Ltd, Oxford., 149, 43-52. https://doi.org/10.1016/j.jsbmb.2015.01.013
Tesić V, Perović M, Lazić D, Kojić S, Smiljanić K, Ruzdijić S, Rakić L, Kanazir S. Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology. 2015;149:43-52. doi:10.1016/j.jsbmb.2015.01.013 .
Tesić, Vesna, Perović, Milka, Lazić, Divna, Kojić, Snežana, Smiljanić, Kosara, Ruzdijić, Sabera, Rakić, Ljubisav, Kanazir, Selma, "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat" in Journal of Steroid Biochemistry and Molecular Biology, 149 (2015):43-52, https://doi.org/10.1016/j.jsbmb.2015.01.013 . .