Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds
Samo za registrovane korisnike
2016
Autori
Duvnjak, DunjaPantić, Milena
Pavlović, Vladimir
Nedović, Viktor
Lević, Steva
Matijašević, Danka
Sknepnek, Aleksandra
Nikšić, Miomir
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC) lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbici...dal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.
Ključne reči:
Submerged cultivation / Mycelium / Mushrooms / Exopolysaccharides / Coriolus versicolor / Antibacterial activityIzvor:
Innovative Food Science & Emerging Technologies, 2016, 34, 1-8Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Usmerena sinteza, struktura i svojstva multifunkcionalnih materijala (RS-MESTD-Basic Research (BR or ON)-172057)
DOI: 10.1016/j.ifset.2015.12.028
ISSN: 1466-8564
WoS: 000375814400001
Scopus: 2-s2.0-84964545776
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Duvnjak, Dunja AU - Pantić, Milena AU - Pavlović, Vladimir AU - Nedović, Viktor AU - Lević, Steva AU - Matijašević, Danka AU - Sknepnek, Aleksandra AU - Nikšić, Miomir PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/969 AB - Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC) lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria. PB - Elsevier Sci Ltd, Oxford T2 - Innovative Food Science & Emerging Technologies T1 - Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds EP - 8 SP - 1 VL - 34 DO - 10.1016/j.ifset.2015.12.028 ER -
@article{ author = "Duvnjak, Dunja and Pantić, Milena and Pavlović, Vladimir and Nedović, Viktor and Lević, Steva and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir", year = "2016", abstract = "Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC) lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Innovative Food Science & Emerging Technologies", title = "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds", pages = "8-1", volume = "34", doi = "10.1016/j.ifset.2015.12.028" }
Duvnjak, D., Pantić, M., Pavlović, V., Nedović, V., Lević, S., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2016). Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies Elsevier Sci Ltd, Oxford., 34, 1-8. https://doi.org/10.1016/j.ifset.2015.12.028
Duvnjak D, Pantić M, Pavlović V, Nedović V, Lević S, Matijašević D, Sknepnek A, Nikšić M. Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies. 2016;34:1-8. doi:10.1016/j.ifset.2015.12.028 .
Duvnjak, Dunja, Pantić, Milena, Pavlović, Vladimir, Nedović, Viktor, Lević, Steva, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds" in Innovative Food Science & Emerging Technologies, 34 (2016):1-8, https://doi.org/10.1016/j.ifset.2015.12.028 . .