TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beskoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1337
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beskoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beskoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević N, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beskoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja, Soković Bajić, Svetlana, Jovanović, Živko, Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beskoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .

TY  - JOUR
AU  - Jakovljević, K.
AU  - Joksović, M.D.
AU  - Matić, Ivana
AU  - Petrović, N.
AU  - Stanojković, T.
AU  - Sladić, D.
AU  - Vujčić, M.
AU  - Janović, B.
AU  - Joksović, L.
AU  - Trifunović, Sara
AU  - Marković, V.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1767
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
PB  - Royal Society of Chemistry
T2  - Medchemcomm
T1  - Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
EP  - 1697
IS  - 10
SP  - 1679
VL  - 9
DO  - 10.1039/c8md00316e
ER  - 

@article{
author = "Jakovljević, K. and Joksović, M.D. and Matić, Ivana and Petrović, N. and Stanojković, T. and Sladić, D. and Vujčić, M. and Janović, B. and Joksović, L. and Trifunović, Sara and Marković, V.",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
publisher = "Royal Society of Chemistry",
journal = "Medchemcomm",
title = "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
pages = "1697-1679",
number = "10",
volume = "9",
doi = "10.1039/c8md00316e"
}

Jakovljević, K., Joksović, M.D., Matić, I., Petrović, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksović, L., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm
Royal Society of Chemistry., 9(10), 1679-1697.
https://doi.org/10.1039/c8md00316e

Jakovljević K, Joksović M, Matić I, Petrović N, Stanojković T, Sladić D, Vujčić M, Janović B, Joksović L, Trifunović S, Marković V. Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm. 2018;9(10):1679-1697.
doi:10.1039/c8md00316e .

Jakovljević, K., Joksović, M.D., Matić, Ivana, Petrović, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksović, L., Trifunović, Sara, Marković, V., "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in Medchemcomm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/c8md00316e . .

TY  - JOUR
AU  - Jakovljević, K.
AU  - Joksović, M.D.
AU  - Matić, Ivana
AU  - Petrović, N.
AU  - Stanojković, T.
AU  - Sladić, D.
AU  - Vujčić, M.
AU  - Janović, B.
AU  - Joksović, L.
AU  - Trifunović, Sara
AU  - Marković, V.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1168
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
PB  - Royal Society of Chemistry
T2  - Medchemcomm
T1  - Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
EP  - 1697
IS  - 10
SP  - 1679
VL  - 9
DO  - 10.1039/c8md00316e
ER  - 

@article{
author = "Jakovljević, K. and Joksović, M.D. and Matić, Ivana and Petrović, N. and Stanojković, T. and Sladić, D. and Vujčić, M. and Janović, B. and Joksović, L. and Trifunović, Sara and Marković, V.",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
publisher = "Royal Society of Chemistry",
journal = "Medchemcomm",
title = "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
pages = "1697-1679",
number = "10",
volume = "9",
doi = "10.1039/c8md00316e"
}

Jakovljević, K., Joksović, M.D., Matić, I., Petrović, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksović, L., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm
Royal Society of Chemistry., 9(10), 1679-1697.
https://doi.org/10.1039/c8md00316e

Jakovljević K, Joksović M, Matić I, Petrović N, Stanojković T, Sladić D, Vujčić M, Janović B, Joksović L, Trifunović S, Marković V. Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in Medchemcomm. 2018;9(10):1679-1697.
doi:10.1039/c8md00316e .

Jakovljević, K., Joksović, M.D., Matić, Ivana, Petrović, N., Stanojković, T., Sladić, D., Vujčić, M., Janović, B., Joksović, L., Trifunović, Sara, Marković, V., "Novel 1,3,4-thiadiazole-chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in Medchemcomm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/c8md00316e . .

TY  - JOUR
AU  - Mihailović, Nevena
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Trifunović, Snežana
AU  - Joksović, Ljubinka
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1058
AB  - Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids
EP  - 8560
IS  - 14
SP  - 8550
VL  - 7
DO  - 10.1039/c6ra28787e
ER  - 

@article{
author = "Mihailović, Nevena and Marković, Violeta and Matić, Ivana Z. and Stanisavljević, Nemanja and Jovanović, Živko and Trifunović, Snežana and Joksović, Ljubinka",
year = "2017",
abstract = "Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids",
pages = "8560-8550",
number = "14",
volume = "7",
doi = "10.1039/c6ra28787e"
}

Mihailović, N., Marković, V., Matić, I. Z., Stanisavljević, N., Jovanović, Ž., Trifunović, S.,& Joksović, L.. (2017). Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances
Royal Soc Chemistry, Cambridge., 7(14), 8550-8560.
https://doi.org/10.1039/c6ra28787e

Mihailović N, Marković V, Matić IZ, Stanisavljević N, Jovanović Ž, Trifunović S, Joksović L. Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances. 2017;7(14):8550-8560.
doi:10.1039/c6ra28787e .

Mihailović, Nevena, Marković, Violeta, Matić, Ivana Z., Stanisavljević, Nemanja, Jovanović, Živko, Trifunović, Snežana, Joksović, Ljubinka, "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids" in RSC Advances, 7, no. 14 (2017):8550-8560,
https://doi.org/10.1039/c6ra28787e . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Matić, Ivana
AU  - Begović, Jelena
AU  - Besu, Irina
AU  - Kojić, Milan
AU  - Đokić, Jelena
AU  - Juranić, Zorica
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/928
AB  - The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases
EP  - 7
SP  - 1
VL  - 63
DO  - 10.1016/j.idairyj.2016.07.009
ER  - 

@article{
author = "Vukotić, Goran and Matić, Ivana and Begović, Jelena and Besu, Irina and Kojić, Milan and Đokić, Jelena and Juranić, Zorica and Strahinić, Ivana",
year = "2016",
abstract = "The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases",
pages = "7-1",
volume = "63",
doi = "10.1016/j.idairyj.2016.07.009"
}

Vukotić, G., Matić, I., Begović, J., Besu, I., Kojić, M., Đokić, J., Juranić, Z.,& Strahinić, I.. (2016). Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 63, 1-7.
https://doi.org/10.1016/j.idairyj.2016.07.009

Vukotić G, Matić I, Begović J, Besu I, Kojić M, Đokić J, Juranić Z, Strahinić I. Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal. 2016;63:1-7.
doi:10.1016/j.idairyj.2016.07.009 .

Vukotić, Goran, Matić, Ivana, Begović, Jelena, Besu, Irina, Kojić, Milan, Đokić, Jelena, Juranić, Zorica, Strahinić, Ivana, "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases" in International Dairy Journal, 63 (2016):1-7,
https://doi.org/10.1016/j.idairyj.2016.07.009 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko
AU  - Cupić, Tihomir
AU  - Dabić, Dragana C.
AU  - Natić, Maja M.
AU  - Tesić, Zivoslav Lj.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/905
AB  - To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities
EP  - 1000
IS  - 6
SP  - 988
VL  - 68
DO  - 10.1080/01635581.2016.1190019
ER  - 

@article{
author = "Stanisavljević, Nemanja and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko and Cupić, Tihomir and Dabić, Dragana C. and Natić, Maja M. and Tesić, Zivoslav Lj.",
year = "2016",
abstract = "To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities",
pages = "1000-988",
number = "6",
volume = "68",
doi = "10.1080/01635581.2016.1190019"
}

Stanisavljević, N., Ilić, M. D., Matić, I. Z., Jovanović, Ž., Cupić, T., Dabić, D. C., Natić, M. M.,& Tesić, Z. Lj.. (2016). Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 68(6), 988-1000.
https://doi.org/10.1080/01635581.2016.1190019

Stanisavljević N, Ilić MD, Matić IZ, Jovanović Ž, Cupić T, Dabić DC, Natić MM, Tesić ZL. Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer-An International Journal. 2016;68(6):988-1000.
doi:10.1080/01635581.2016.1190019 .

Stanisavljević, Nemanja, Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko, Cupić, Tihomir, Dabić, Dragana C., Natić, Maja M., Tesić, Zivoslav Lj., "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities" in Nutrition and Cancer-An International Journal, 68, no. 6 (2016):988-1000,
https://doi.org/10.1080/01635581.2016.1190019 . .

TY  - JOUR
AU  - Opsenica, Dejan M.
AU  - Milovanović, Jelena
AU  - Matić, Ivana Z.
AU  - Stajner, Tijana
AU  - Knezevic-Usaj, Slavica
AU  - Đurković-Đaković, Olgica
AU  - Solaja, Bogdan A.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/833
AB  - New cyclohexylidene 1,2,4,5-tetraoxanes with polar guanidine and urea based groups were synthesized and evaluated for their antimalarial activity against chloroquine resistant and susceptible Plasmodium falciparum strains. The derivatives showed moderate, nM range antimalarial activities and low cytotoxicity. The N-phenylurea derivative 24 exhibited the best resistance indices (RIW2 = 0.44, RITM91C235 = 0.80) and was not toxic against human normal peripheral blood mononuclear cells (IC50  gt  200 mu M). Seven derivatives were tested in vitro against four human cancer cell lines and they demonstrated high selectivity toward leukaemia K562 cells. One compound, derivative 21 with a primary amino group, was the first tetraoxane tested in vivo against Toxoplasma gondii as another apicomplexan parasite. Subcutaneous administration at a dose of 10 mg kg(-1) day(-1) for 8 days allowed the survival of 20 % of infected mice, thus demonstrating the high potential of tetraoxanes for the treatment of apicomplexan parasites.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules
EP  - +
IS  - 11
SP  - 1339
VL  - 80
DO  - 10.2298/JSC150430063O
ER  - 

@article{
author = "Opsenica, Dejan M. and Milovanović, Jelena and Matić, Ivana Z. and Stajner, Tijana and Knezevic-Usaj, Slavica and Đurković-Đaković, Olgica and Solaja, Bogdan A.",
year = "2015",
abstract = "New cyclohexylidene 1,2,4,5-tetraoxanes with polar guanidine and urea based groups were synthesized and evaluated for their antimalarial activity against chloroquine resistant and susceptible Plasmodium falciparum strains. The derivatives showed moderate, nM range antimalarial activities and low cytotoxicity. The N-phenylurea derivative 24 exhibited the best resistance indices (RIW2 = 0.44, RITM91C235 = 0.80) and was not toxic against human normal peripheral blood mononuclear cells (IC50  gt  200 mu M). Seven derivatives were tested in vitro against four human cancer cell lines and they demonstrated high selectivity toward leukaemia K562 cells. One compound, derivative 21 with a primary amino group, was the first tetraoxane tested in vivo against Toxoplasma gondii as another apicomplexan parasite. Subcutaneous administration at a dose of 10 mg kg(-1) day(-1) for 8 days allowed the survival of 20 % of infected mice, thus demonstrating the high potential of tetraoxanes for the treatment of apicomplexan parasites.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules",
pages = "+-1339",
number = "11",
volume = "80",
doi = "10.2298/JSC150430063O"
}

Opsenica, D. M., Milovanović, J., Matić, I. Z., Stajner, T., Knezevic-Usaj, S., Đurković-Đaković, O.,& Solaja, B. A.. (2015). Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 80(11), 1339-+.
https://doi.org/10.2298/JSC150430063O

Opsenica DM, Milovanović J, Matić IZ, Stajner T, Knezevic-Usaj S, Đurković-Đaković O, Solaja BA. Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules. in Journal of the Serbian Chemical Society. 2015;80(11):1339-+.
doi:10.2298/JSC150430063O .

Opsenica, Dejan M., Milovanović, Jelena, Matić, Ivana Z., Stajner, Tijana, Knezevic-Usaj, Slavica, Đurković-Đaković, Olgica, Solaja, Bogdan A., "Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1339-+,
https://doi.org/10.2298/JSC150430063O . .