TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Zille, Andrea
AU  - Ribeiro, Ana Isabel
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2024
UR  - https://www.mdpi.com/1420-3049/29/7/1447
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2335
AB  - The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.
PB  - MDPI
T2  - Molecules
T2  - Molecules
T1  - Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies
IS  - 7
SP  - 1447
VL  - 29
DO  - 10.3390/molecules29071447
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Zille, Andrea and Ribeiro, Ana Isabel and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2024",
abstract = "The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.",
publisher = "MDPI",
journal = "Molecules, Molecules",
title = "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies",
number = "7",
pages = "1447",
volume = "29",
doi = "10.3390/molecules29071447"
}

Krkobabić, A., Radetić, M., Zille, A., Ribeiro, A. I., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2024). Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules
MDPI., 29(7), 1447.
https://doi.org/10.3390/molecules29071447

Krkobabić A, Radetić M, Zille A, Ribeiro AI, Tadić V, Ilić-Tomić T, Marković D. Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules. 2024;29(7):1447.
doi:10.3390/molecules29071447 .

Krkobabić, Ana, Radetić, Maja, Zille, Andrea, Ribeiro, Ana Isabel, Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies" in Molecules, 29, no. 7 (2024):1447,
https://doi.org/10.3390/molecules29071447 . .

TY  - CONF
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Stevanović, Milena
AU  - Nenadović, Marija
AU  - Ilić-Tomić, Tatjana
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2026
AB  - Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Microbial live interactions with textiles
EP  - 835
EP  - 835
SP  - 835
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2026
ER  - 

@conference{
author = "Janković, Vukašin and Nikodinović-Runić, Jasmina and Radetić, Maja and Marković, Darka and Stevanović, Milena and Nenadović, Marija and Ilić-Tomić, Tatjana",
year = "2023",
abstract = "Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Microbial live interactions with textiles",
pages = "835-835-835",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2026"
}

Janković, V., Nikodinović-Runić, J., Radetić, M., Marković, D., Stevanović, M., Nenadović, M.,& Ilić-Tomić, T.. (2023). Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists, 10, 835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026

Janković V, Nikodinović-Runić J, Radetić M, Marković D, Stevanović M, Nenadović M, Ilić-Tomić T. Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists. 2023;10:835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026 .

Janković, Vukašin, Nikodinović-Runić, Jasmina, Radetić, Maja, Marković, Darka, Stevanović, Milena, Nenadović, Marija, Ilić-Tomić, Tatjana, "Microbial live interactions with textiles" in FEMS2023 Congress of European Microbiologists, 10 (2023):835-835,
https://hdl.handle.net/21.15107/rcub_imagine_2026 .

TY  - JOUR
AU  - Marković, Darka
AU  - Petkovska, Jovana
AU  - Mladenović, Nina
AU  - Radoičić, Marija
AU  - Rodriguez-Melendez, Danixa
AU  - Ilić-Tomić, Tatjana
AU  - Radetić, Maja
AU  - Grunlan, Jaime
AU  - Jordanov, Igor
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1794
AB  - Poor antimicrobial activity and lack of protection against UV irradiation are weaknesses of cotton

textiles. In an attempt to impart superior antimicrobial and UV-protective properties to cotton, layer-by-
layer deposition of chitosan and magnesium lignosulfonate, and in situ synthesis of silver (Ag)

nanoparticles (NPs), was performed. Lignin, in a chitosan/lignin multilayer, simultaneously acts as a UV
protective macromolecule and natural reducing and stabilizing agent, allowing formation of Ag NPs. Four
bilayers of this coating is sufficient for fabrication of a chitosan/lignin/Ag-NP textile nanocomposite

treatment with 50+ UV protection. 100% reduction of Gram-negative bacteria Escherichia coli, gram-
positive bacteria Staphylococcus aureus, and yeast Candida albicans can be achieved with a 12-bilayer

coating, when 20 mM silver nitrate solution and sodium borohydride are applied. On the other hand,
four bilayers impregnated with Ag NPs, using 10 mM silver nitrate solution, provides sufficient
antimicrobial activity independent of an added reducing agent. This treatment exhibits no inhibition of
human keratinocyte cells growth on the skin, indicating low cytotoxicity.
PB  - Wiley Periodicals, LLC.
T2  - Journal of Applied Polymer Science
T1  - Antimicrobial and UV protective chitosan/lignin multilayer nanocoating with immobilized silver nanoparticles
DO  - 10.1002/app.53823
ER  - 

@article{
author = "Marković, Darka and Petkovska, Jovana and Mladenović, Nina and Radoičić, Marija and Rodriguez-Melendez, Danixa and Ilić-Tomić, Tatjana and Radetić, Maja and Grunlan, Jaime and Jordanov, Igor",
year = "2023",
abstract = "Poor antimicrobial activity and lack of protection against UV irradiation are weaknesses of cotton

textiles. In an attempt to impart superior antimicrobial and UV-protective properties to cotton, layer-by-
layer deposition of chitosan and magnesium lignosulfonate, and in situ synthesis of silver (Ag)

nanoparticles (NPs), was performed. Lignin, in a chitosan/lignin multilayer, simultaneously acts as a UV
protective macromolecule and natural reducing and stabilizing agent, allowing formation of Ag NPs. Four
bilayers of this coating is sufficient for fabrication of a chitosan/lignin/Ag-NP textile nanocomposite

treatment with 50+ UV protection. 100% reduction of Gram-negative bacteria Escherichia coli, gram-
positive bacteria Staphylococcus aureus, and yeast Candida albicans can be achieved with a 12-bilayer

coating, when 20 mM silver nitrate solution and sodium borohydride are applied. On the other hand,
four bilayers impregnated with Ag NPs, using 10 mM silver nitrate solution, provides sufficient
antimicrobial activity independent of an added reducing agent. This treatment exhibits no inhibition of
human keratinocyte cells growth on the skin, indicating low cytotoxicity.",
publisher = "Wiley Periodicals, LLC.",
journal = "Journal of Applied Polymer Science",
title = "Antimicrobial and UV protective chitosan/lignin multilayer nanocoating with immobilized silver nanoparticles",
doi = "10.1002/app.53823"
}

Marković, D., Petkovska, J., Mladenović, N., Radoičić, M., Rodriguez-Melendez, D., Ilić-Tomić, T., Radetić, M., Grunlan, J.,& Jordanov, I.. (2023). Antimicrobial and UV protective chitosan/lignin multilayer nanocoating with immobilized silver nanoparticles. in Journal of Applied Polymer Science
Wiley Periodicals, LLC...
https://doi.org/10.1002/app.53823

Marković D, Petkovska J, Mladenović N, Radoičić M, Rodriguez-Melendez D, Ilić-Tomić T, Radetić M, Grunlan J, Jordanov I. Antimicrobial and UV protective chitosan/lignin multilayer nanocoating with immobilized silver nanoparticles. in Journal of Applied Polymer Science. 2023;.
doi:10.1002/app.53823 .

Marković, Darka, Petkovska, Jovana, Mladenović, Nina, Radoičić, Marija, Rodriguez-Melendez, Danixa, Ilić-Tomić, Tatjana, Radetić, Maja, Grunlan, Jaime, Jordanov, Igor, "Antimicrobial and UV protective chitosan/lignin multilayer nanocoating with immobilized silver nanoparticles" in Journal of Applied Polymer Science (2023),
https://doi.org/10.1002/app.53823 . .

TY  - JOUR
AU  - Kovačević, Aleksandar
AU  - Radoičić, Marija
AU  - Marković, Darka
AU  - Ponjavić, Marijana
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2352186423001669
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1840
AB  - Loose assemblies of cellulose fibers could be employed in the clean-up of oil spills, but the difficulty to separate them from the spill after use makes them impractical. In an effort to tackle this issue, a non-woven sorbent based on recycled jute fibers from the carpet industry was developed. To enhance the porosity and hydrophobicity/oleophilicity of the sorbent, carbonization in an inert atmosphere was carried out. A comparison in oil sorption performance between non-carbonized and carbonized sorbents was made by evaluating the oil capacity in a water medium, buoyancy, oil retention and reusability. Carbonization of sorbent resulted in more than doubled oil sorption capacity independent of oil viscosity. Oil viscosity did not affect the oil sorption capacity of non-carbonized sorbent. Carbonized sorbent showed superior buoyancy in water even after 24 hours, oil retention of approximately 60–80% after 3 hours and only 12–20% decline of oil sorption capacity after five repeated sorption/desorption trials. However, the ability of oiled non-carbonized sorbent to efficiently biodegrade in model compost (up to 45% weight loss after 10 weeks) makes it a sustainable candidate for oil spill clean-up.
T2  - Environmental Technology & Innovation
T2  - Environmental Technology & InnovationEnvironmental Technology & Innovation
T1  - Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation
SP  - 103170
VL  - 31
DO  - 10.1016/j.eti.2023.103170
ER  - 

@article{
author = "Kovačević, Aleksandar and Radoičić, Marija and Marković, Darka and Ponjavić, Marijana and Nikodinović-Runić, Jasmina and Radetić, Maja",
year = "2023",
abstract = "Loose assemblies of cellulose fibers could be employed in the clean-up of oil spills, but the difficulty to separate them from the spill after use makes them impractical. In an effort to tackle this issue, a non-woven sorbent based on recycled jute fibers from the carpet industry was developed. To enhance the porosity and hydrophobicity/oleophilicity of the sorbent, carbonization in an inert atmosphere was carried out. A comparison in oil sorption performance between non-carbonized and carbonized sorbents was made by evaluating the oil capacity in a water medium, buoyancy, oil retention and reusability. Carbonization of sorbent resulted in more than doubled oil sorption capacity independent of oil viscosity. Oil viscosity did not affect the oil sorption capacity of non-carbonized sorbent. Carbonized sorbent showed superior buoyancy in water even after 24 hours, oil retention of approximately 60–80% after 3 hours and only 12–20% decline of oil sorption capacity after five repeated sorption/desorption trials. However, the ability of oiled non-carbonized sorbent to efficiently biodegrade in model compost (up to 45% weight loss after 10 weeks) makes it a sustainable candidate for oil spill clean-up.",
journal = "Environmental Technology & Innovation, Environmental Technology & InnovationEnvironmental Technology & Innovation",
title = "Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation",
pages = "103170",
volume = "31",
doi = "10.1016/j.eti.2023.103170"
}

Kovačević, A., Radoičić, M., Marković, D., Ponjavić, M., Nikodinović-Runić, J.,& Radetić, M.. (2023). Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation. in Environmental Technology & Innovation, 31, 103170.
https://doi.org/10.1016/j.eti.2023.103170

Kovačević A, Radoičić M, Marković D, Ponjavić M, Nikodinović-Runić J, Radetić M. Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation. in Environmental Technology & Innovation. 2023;31:103170.
doi:10.1016/j.eti.2023.103170 .

Kovačević, Aleksandar, Radoičić, Marija, Marković, Darka, Ponjavić, Marijana, Nikodinović-Runić, Jasmina, Radetić, Maja, "Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation" in Environmental Technology & Innovation, 31 (2023):103170,
https://doi.org/10.1016/j.eti.2023.103170 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Tseng, Hsiang-Han
AU  - Nunney, Tim S.
AU  - Tadić, Vanja
AU  - Ilic-Tomic, Tatjana
AU  - Marković, Darka
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1668
AB  - The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.
T2  - Applied Surface Science
T2  - Applied Surface ScienceApplied Surface Science
T1  - Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract
SP  - 155612
VL  - 611
DO  - 10.1016/j.apsusc.2022.155612
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Tseng, Hsiang-Han and Nunney, Tim S. and Tadić, Vanja and Ilic-Tomic, Tatjana and Marković, Darka",
year = "2023",
abstract = "The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.",
journal = "Applied Surface Science, Applied Surface ScienceApplied Surface Science",
title = "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract",
pages = "155612",
volume = "611",
doi = "10.1016/j.apsusc.2022.155612"
}

Krkobabić, A., Radetić, M., Tseng, H., Nunney, T. S., Tadić, V., Ilic-Tomic, T.,& Marković, D.. (2023). Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science, 611, 155612.
https://doi.org/10.1016/j.apsusc.2022.155612

Krkobabić A, Radetić M, Tseng H, Nunney TS, Tadić V, Ilic-Tomic T, Marković D. Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science. 2023;611:155612.
doi:10.1016/j.apsusc.2022.155612 .

Krkobabić, Ana, Radetić, Maja, Tseng, Hsiang-Han, Nunney, Tim S., Tadić, Vanja, Ilic-Tomic, Tatjana, Marković, Darka, "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract" in Applied Surface Science, 611 (2023):155612,
https://doi.org/10.1016/j.apsusc.2022.155612 . .

TY  - JOUR
AU  - Tomsić, Brigita
AU  - Marković, Darka
AU  - Janković, Vukašin
AU  - Simoncić, Barbara
AU  - Nikodinović-Runić, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Radetić, Maja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1539
AB  - Sustainable biodegradation of cellulose fibers is critical for composting after the end of a product's life. In this study, we aimed at investigating the effect of in situ synthesized CuO/Cu2O nanoparticles (NPs) with biocidal concentration on the biodegradation behavior of cotton fibers pretreated with 1,2,3,4-butanetetracarboxylic acid (BTCA) and succinic acid (SUC). Biodegradation of the fibers was evaluated by soil burial tests in garden soil and in model compost after different soil burial times. The results showed that the application of BTCA, SUC, and CuO/Cu2O NPs did not affect the hydrophilicity of the samples and allowed a smooth biodegradation process. The morphological and chemical changes during biodegradation, evaluated by FESEM and FTIR analyses, showed that the presence of CuO/Cu2O NPs slightly hindered biodegradation of the fibers after 18 days in soil. However, biodegradation was much faster in the model compost, where all samples, regardless of their chemical modification, almost completely degraded after only 11 days. Intense microbial growth on the surface of all samples after nine days of burial in garden soil and model compost was confirmed by the presence of proteins produced by the microorganisms. The total number of microorganisms in the garden soil remained almost unchanged and increased in the model compost after the burial test. The only exception was the sample with the highest concentration of CuO/Cu2O NPs, which caused a reduction in microbial growth but not complete growth inhibition. These results clearly showed that during material degradation, the cellulosic material supporting microbial growth prevailed over the suppression of microbial growth by CuO/Cu2O NPs.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Biodegradation of cellulose fibers functionalized with CuO/Cu2O nanoparticles in combination with polycarboxylic acids
EP  - 302
IS  - 1
SP  - 287
VL  - 29
DO  - 10.1007/s10570-021-04296-6
ER  - 

@article{
author = "Tomsić, Brigita and Marković, Darka and Janković, Vukašin and Simoncić, Barbara and Nikodinović-Runić, Jasmina and Ilić-Tomić, Tatjana and Radetić, Maja",
year = "2022",
abstract = "Sustainable biodegradation of cellulose fibers is critical for composting after the end of a product's life. In this study, we aimed at investigating the effect of in situ synthesized CuO/Cu2O nanoparticles (NPs) with biocidal concentration on the biodegradation behavior of cotton fibers pretreated with 1,2,3,4-butanetetracarboxylic acid (BTCA) and succinic acid (SUC). Biodegradation of the fibers was evaluated by soil burial tests in garden soil and in model compost after different soil burial times. The results showed that the application of BTCA, SUC, and CuO/Cu2O NPs did not affect the hydrophilicity of the samples and allowed a smooth biodegradation process. The morphological and chemical changes during biodegradation, evaluated by FESEM and FTIR analyses, showed that the presence of CuO/Cu2O NPs slightly hindered biodegradation of the fibers after 18 days in soil. However, biodegradation was much faster in the model compost, where all samples, regardless of their chemical modification, almost completely degraded after only 11 days. Intense microbial growth on the surface of all samples after nine days of burial in garden soil and model compost was confirmed by the presence of proteins produced by the microorganisms. The total number of microorganisms in the garden soil remained almost unchanged and increased in the model compost after the burial test. The only exception was the sample with the highest concentration of CuO/Cu2O NPs, which caused a reduction in microbial growth but not complete growth inhibition. These results clearly showed that during material degradation, the cellulosic material supporting microbial growth prevailed over the suppression of microbial growth by CuO/Cu2O NPs.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Biodegradation of cellulose fibers functionalized with CuO/Cu2O nanoparticles in combination with polycarboxylic acids",
pages = "302-287",
number = "1",
volume = "29",
doi = "10.1007/s10570-021-04296-6"
}

Tomsić, B., Marković, D., Janković, V., Simoncić, B., Nikodinović-Runić, J., Ilić-Tomić, T.,& Radetić, M.. (2022). Biodegradation of cellulose fibers functionalized with CuO/Cu2O nanoparticles in combination with polycarboxylic acids. in Cellulose
Springer, Dordrecht., 29(1), 287-302.
https://doi.org/10.1007/s10570-021-04296-6

Tomsić B, Marković D, Janković V, Simoncić B, Nikodinović-Runić J, Ilić-Tomić T, Radetić M. Biodegradation of cellulose fibers functionalized with CuO/Cu2O nanoparticles in combination with polycarboxylic acids. in Cellulose. 2022;29(1):287-302.
doi:10.1007/s10570-021-04296-6 .

Tomsić, Brigita, Marković, Darka, Janković, Vukašin, Simoncić, Barbara, Nikodinović-Runić, Jasmina, Ilić-Tomić, Tatjana, Radetić, Maja, "Biodegradation of cellulose fibers functionalized with CuO/Cu2O nanoparticles in combination with polycarboxylic acids" in Cellulose, 29, no. 1 (2022):287-302,
https://doi.org/10.1007/s10570-021-04296-6 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Marković, Darka
AU  - Kovacević, Aleksandar
AU  - Tadić, Vanja
AU  - Radoicić, Marija
AU  - Barudzija, Tatjana
AU  - Ilić-Tomić, Tatjana
AU  - Radetić, Maja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1552
AB  - The aim of this study was to develop antimicrobial nanocomposite textile material comprising of Cu-based nanostructures synthesized on oxidized cotton fabric using Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry leaves) as a green reducing agent for adsorbed Cu2+-ions. In order to provide sufficient number of carboxyl groups for complexation with Cu2+-ions a two-step oxidation process with NaIO4 and NaClO2 was carried out. The influence of NaIO4 concentration on content of carboxyl groups and Cu-based nanoparticles was studied by FTIR and AAS. HPLC analysis identified the gallic acid known as a reducing agent in bearberry leaves extract. FESEM and XRD analyses revealed that using bearberry leaves extract and gallic acid solution as reducing agents led to a formation of spherical Cu2O/CuO nanoparticles and CuO nanosheets, respectively. These nanoparticles and nanosheets provided excellent antibacterial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus. Cytotoxicity on human keratinocyte cells was shown to depend on their copper content.
PB  - Korean Fiber Soc, Seoul
T2  - Fibers and Polymers
T1  - Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract
EP  - 966
IS  - 4
SP  - 954
VL  - 23
DO  - 10.1007/s12221-022-4639-5
ER  - 

@article{
author = "Krkobabić, Ana and Marković, Darka and Kovacević, Aleksandar and Tadić, Vanja and Radoicić, Marija and Barudzija, Tatjana and Ilić-Tomić, Tatjana and Radetić, Maja",
year = "2022",
abstract = "The aim of this study was to develop antimicrobial nanocomposite textile material comprising of Cu-based nanostructures synthesized on oxidized cotton fabric using Arctostaphylos uva-ursi (L.) Spreng., Ericaceae (bearberry leaves) as a green reducing agent for adsorbed Cu2+-ions. In order to provide sufficient number of carboxyl groups for complexation with Cu2+-ions a two-step oxidation process with NaIO4 and NaClO2 was carried out. The influence of NaIO4 concentration on content of carboxyl groups and Cu-based nanoparticles was studied by FTIR and AAS. HPLC analysis identified the gallic acid known as a reducing agent in bearberry leaves extract. FESEM and XRD analyses revealed that using bearberry leaves extract and gallic acid solution as reducing agents led to a formation of spherical Cu2O/CuO nanoparticles and CuO nanosheets, respectively. These nanoparticles and nanosheets provided excellent antibacterial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus. Cytotoxicity on human keratinocyte cells was shown to depend on their copper content.",
publisher = "Korean Fiber Soc, Seoul",
journal = "Fibers and Polymers",
title = "Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract",
pages = "966-954",
number = "4",
volume = "23",
doi = "10.1007/s12221-022-4639-5"
}

Krkobabić, A., Marković, D., Kovacević, A., Tadić, V., Radoicić, M., Barudzija, T., Ilić-Tomić, T.,& Radetić, M.. (2022). Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract. in Fibers and Polymers
Korean Fiber Soc, Seoul., 23(4), 954-966.
https://doi.org/10.1007/s12221-022-4639-5

Krkobabić A, Marković D, Kovacević A, Tadić V, Radoicić M, Barudzija T, Ilić-Tomić T, Radetić M. Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract. in Fibers and Polymers. 2022;23(4):954-966.
doi:10.1007/s12221-022-4639-5 .

Krkobabić, Ana, Marković, Darka, Kovacević, Aleksandar, Tadić, Vanja, Radoicić, Marija, Barudzija, Tatjana, Ilić-Tomić, Tatjana, Radetić, Maja, "Antimicrobial Nanocomposites Based on Oxidized Cotton Fabric and in situ Biosynthesized Copper Oxides Nanostructures Using Bearberry Leaves Extract" in Fibers and Polymers, 23, no. 4 (2022):954-966,
https://doi.org/10.1007/s12221-022-4639-5 . .

TY  - JOUR
AU  - Janković, Vukašin
AU  - Marković, Darka
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
AU  - Ilic-Tomic, Tatjana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1665
AB  - Given the environmental burden of textile industry, especially of dyeing processes and the volume of synthetic dyes and surfactants, the intensive development of the greener approaches is under way. Herein, an environmentaly-friendly dyeing of polyamide (PA) and PA/Elastane (PA/EA) knits using live bacterial approach in water environment, completely eliminating usage of textile auxiliaries is described. A total of 12 pigment-producing Streptomyces strains were isolated and purified from soil and rizoshere or bark of smoke tree Cotinus coggygria samples. The antibacterial, antifungal and cytotoxic effects of crude bacterial extracts were tested. Antimicrobial effect was obtained by the majority of extracts but only two streptomycetes extracts, 11–5 and BPS51, showed moderate cytotoxicity against HaCaT human cell line. This was the reason to select 11–5 and BPS51 strains for the dyeing of the textile materials. Excellent properties of dyeing wool, silk and PA are achieved initially using live cultures, and the bioprocess is optimized on commercial PA and PA/EA knits used for stockings production. Satisfactory coloration of both knits is achieved with dynamic conditions (culture shaking at 180 rpm over 5–14 days at 30 ºC) giving the best coloration results, except in the case of the PA sample dyed with a bacterial strain 11–5. The prolongation of dyeing time leads to higher color yields independently of fabric and bacteria strain. Although the color differences between the samples before and after washing are observed, washing fastness after three washing cycles can be considered as satisfactory.
T2  - World Journal of Microbiology and Biotechnology
T2  - World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol
T1  - Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains
IS  - 1
SP  - 32
VL  - 39
DO  - 10.1007/s11274-022-03473-4
ER  - 

@article{
author = "Janković, Vukašin and Marković, Darka and Nikodinović-Runić, Jasmina and Radetić, Maja and Ilic-Tomic, Tatjana",
year = "2022",
abstract = "Given the environmental burden of textile industry, especially of dyeing processes and the volume of synthetic dyes and surfactants, the intensive development of the greener approaches is under way. Herein, an environmentaly-friendly dyeing of polyamide (PA) and PA/Elastane (PA/EA) knits using live bacterial approach in water environment, completely eliminating usage of textile auxiliaries is described. A total of 12 pigment-producing Streptomyces strains were isolated and purified from soil and rizoshere or bark of smoke tree Cotinus coggygria samples. The antibacterial, antifungal and cytotoxic effects of crude bacterial extracts were tested. Antimicrobial effect was obtained by the majority of extracts but only two streptomycetes extracts, 11–5 and BPS51, showed moderate cytotoxicity against HaCaT human cell line. This was the reason to select 11–5 and BPS51 strains for the dyeing of the textile materials. Excellent properties of dyeing wool, silk and PA are achieved initially using live cultures, and the bioprocess is optimized on commercial PA and PA/EA knits used for stockings production. Satisfactory coloration of both knits is achieved with dynamic conditions (culture shaking at 180 rpm over 5–14 days at 30 ºC) giving the best coloration results, except in the case of the PA sample dyed with a bacterial strain 11–5. The prolongation of dyeing time leads to higher color yields independently of fabric and bacteria strain. Although the color differences between the samples before and after washing are observed, washing fastness after three washing cycles can be considered as satisfactory.",
journal = "World Journal of Microbiology and Biotechnology, World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol",
title = "Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains",
number = "1",
pages = "32",
volume = "39",
doi = "10.1007/s11274-022-03473-4"
}

Janković, V., Marković, D., Nikodinović-Runić, J., Radetić, M.,& Ilic-Tomic, T.. (2022). Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains. in World Journal of Microbiology and Biotechnology, 39(1), 32.
https://doi.org/10.1007/s11274-022-03473-4

Janković V, Marković D, Nikodinović-Runić J, Radetić M, Ilic-Tomic T. Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains. in World Journal of Microbiology and Biotechnology. 2022;39(1):32.
doi:10.1007/s11274-022-03473-4 .

Janković, Vukašin, Marković, Darka, Nikodinović-Runić, Jasmina, Radetić, Maja, Ilic-Tomic, Tatjana, "Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains" in World Journal of Microbiology and Biotechnology, 39, no. 1 (2022):32,
https://doi.org/10.1007/s11274-022-03473-4 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Vasiljević, Jelena
AU  - Asanin, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Tomsić, Brigita
AU  - Jokić, Bojan
AU  - Mitrić, Miodrag
AU  - Simoncić, Barbara
AU  - Mišić, Dušan
AU  - Radetić, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1311
AB  - A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol-gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol-gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO(4)solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negativeEscherichia coliATCC 25922,E. coliATCC BAA 2469, andKlebsiella pneumoniaeATCC BAA 2146, and Gram-positive bacteriaStaphylococcus aureusATCC 25923,S. aureusATCC 43300 and yeastCandida albicansATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.
PB  - Wiley, Hoboken
T2  - Journal of Applied Polymer Science
T1  - The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions
IS  - 40
VL  - 137
DO  - 10.1002/app.49194
ER  - 

@article{
author = "Marković, Darka and Vasiljević, Jelena and Asanin, Jelena and Ilić-Tomić, Tatjana and Tomsić, Brigita and Jokić, Bojan and Mitrić, Miodrag and Simoncić, Barbara and Mišić, Dušan and Radetić, Maja",
year = "2020",
abstract = "A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics-resistant bacteria and yeast was developed. The impregnation process includes the sol-gel treatment of fabric with (3-aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu2O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu2O NPs was based on the adsorption of Cu2+-ions by the introduced amino groups of the sol-gel coating. The adsorbed Cu2+-ions are subsequently reduced in the alkaline solution of NaBH4. X-ray diffraction measurements confirmed the formation of CuO/Cu2O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO(4)solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram-negativeEscherichia coliATCC 25922,E. coliATCC BAA 2469, andKlebsiella pneumoniaeATCC BAA 2146, and Gram-positive bacteriaStaphylococcus aureusATCC 25923,S. aureusATCC 43300 and yeastCandida albicansATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu2+-ions from the fabrics into physiological saline solution was obtained.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions",
number = "40",
volume = "137",
doi = "10.1002/app.49194"
}

Marković, D., Vasiljević, J., Asanin, J., Ilić-Tomić, T., Tomsić, B., Jokić, B., Mitrić, M., Simoncić, B., Mišić, D.,& Radetić, M.. (2020). The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science
Wiley, Hoboken., 137(40).
https://doi.org/10.1002/app.49194

Marković D, Vasiljević J, Asanin J, Ilić-Tomić T, Tomsić B, Jokić B, Mitrić M, Simoncić B, Mišić D, Radetić M. The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions. in Journal of Applied Polymer Science. 2020;137(40).
doi:10.1002/app.49194 .

Marković, Darka, Vasiljević, Jelena, Asanin, Jelena, Ilić-Tomić, Tatjana, Tomsić, Brigita, Jokić, Bojan, Mitrić, Miodrag, Simoncić, Barbara, Mišić, Dušan, Radetić, Maja, "The influence of coating with aminopropyl triethoxysilane and CuO/Cu2O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions" in Journal of Applied Polymer Science, 137, no. 40 (2020),
https://doi.org/10.1002/app.49194 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Tseng, Hsiang-Han
AU  - Nunney, Tim
AU  - Radoicić, Marija
AU  - Ilić-Tomić, Tatjana
AU  - Radetić, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1351
AB  - The objective of this study was to develop novel antimicrobial nanocomposite material based on polypropylene (PP) non-woven fabric, biopolymer alginate and copper oxides nanoparticles. In order to introduce polar groups onto the surface of PP fibers necessary for binding of alginate, non-woven fabric was activated by corona discharge. Carboxylate groups of alginate were further utilized for binding of Cu2+-ions which were reduced with sodium borohydride as a conventional and ascorbic acid as a green reducing agent. Characteristic morphological and chemical changes induced by corona activation and alginate impregnation were confirmed by FTIR, XPS and FESEM analyses. AAS measurements showed that the amounts of generated nanoparticles depend on applied reducing agent and the concentration of precursor salt. XPS analysis suggested that nanoparticles were mixture of copper (I) and (II) oxides. XPS depth profiling gave an exceptional insight into chemical changes within the thin layer on the fiber surface and formation of certain interfaces induced by each treatment. All fabricated nanocomposites provided excellent antimicrobial activity against Gram-negative bacteria E. coli, Gram-positive bacteria S. aureus and yeast C. albicans. The cytotoxicity assay indicated that maximum amount of Cu2+-ions released from synthetized nanocomposite within 24 h was not cytotoxic to human keratinocyte (HaCaT) cells.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Novel antimicrobial nanocomposite based on polypropylene non-woven fabric, biopolymer alginate and copper oxides nanoparticles
VL  - 527
DO  - 10.1016/j.apsusc.2020.146829
ER  - 

@article{
author = "Marković, Darka and Tseng, Hsiang-Han and Nunney, Tim and Radoicić, Marija and Ilić-Tomić, Tatjana and Radetić, Maja",
year = "2020",
abstract = "The objective of this study was to develop novel antimicrobial nanocomposite material based on polypropylene (PP) non-woven fabric, biopolymer alginate and copper oxides nanoparticles. In order to introduce polar groups onto the surface of PP fibers necessary for binding of alginate, non-woven fabric was activated by corona discharge. Carboxylate groups of alginate were further utilized for binding of Cu2+-ions which were reduced with sodium borohydride as a conventional and ascorbic acid as a green reducing agent. Characteristic morphological and chemical changes induced by corona activation and alginate impregnation were confirmed by FTIR, XPS and FESEM analyses. AAS measurements showed that the amounts of generated nanoparticles depend on applied reducing agent and the concentration of precursor salt. XPS analysis suggested that nanoparticles were mixture of copper (I) and (II) oxides. XPS depth profiling gave an exceptional insight into chemical changes within the thin layer on the fiber surface and formation of certain interfaces induced by each treatment. All fabricated nanocomposites provided excellent antimicrobial activity against Gram-negative bacteria E. coli, Gram-positive bacteria S. aureus and yeast C. albicans. The cytotoxicity assay indicated that maximum amount of Cu2+-ions released from synthetized nanocomposite within 24 h was not cytotoxic to human keratinocyte (HaCaT) cells.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Novel antimicrobial nanocomposite based on polypropylene non-woven fabric, biopolymer alginate and copper oxides nanoparticles",
volume = "527",
doi = "10.1016/j.apsusc.2020.146829"
}

Marković, D., Tseng, H., Nunney, T., Radoicić, M., Ilić-Tomić, T.,& Radetić, M.. (2020). Novel antimicrobial nanocomposite based on polypropylene non-woven fabric, biopolymer alginate and copper oxides nanoparticles. in Applied Surface Science
Elsevier, Amsterdam., 527.
https://doi.org/10.1016/j.apsusc.2020.146829

Marković D, Tseng H, Nunney T, Radoicić M, Ilić-Tomić T, Radetić M. Novel antimicrobial nanocomposite based on polypropylene non-woven fabric, biopolymer alginate and copper oxides nanoparticles. in Applied Surface Science. 2020;527.
doi:10.1016/j.apsusc.2020.146829 .

Marković, Darka, Tseng, Hsiang-Han, Nunney, Tim, Radoicić, Marija, Ilić-Tomić, Tatjana, Radetić, Maja, "Novel antimicrobial nanocomposite based on polypropylene non-woven fabric, biopolymer alginate and copper oxides nanoparticles" in Applied Surface Science, 527 (2020),
https://doi.org/10.1016/j.apsusc.2020.146829 . .