TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Mark, Kevin J.
AU  - Dragičević, Sandra
AU  - Babić, Tamara
AU  - Milošević, Katarina
AU  - Nestorović, Branimir
AU  - Beskoski, Vladimir
PY  - 2023
UR  - https://www.eurekaselect.com/article/129606
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2058
AB  - Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p
T2  - Current Proteomics
T1  - Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma
EP  - 18
IS  - 1
SP  - 12
VL  - 20
DO  - 10.2174/1570164620666230220112500
ER  - 

@article{
author = "Nikolić, Aleksandra and Mark, Kevin J. and Dragičević, Sandra and Babić, Tamara and Milošević, Katarina and Nestorović, Branimir and Beskoski, Vladimir",
year = "2023",
abstract = "Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p",
journal = "Current Proteomics",
title = "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma",
pages = "18-12",
number = "1",
volume = "20",
doi = "10.2174/1570164620666230220112500"
}

Nikolić, A., Mark, K. J., Dragičević, S., Babić, T., Milošević, K., Nestorović, B.,& Beskoski, V.. (2023). Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics, 20(1), 12-18.
https://doi.org/10.2174/1570164620666230220112500

Nikolić A, Mark KJ, Dragičević S, Babić T, Milošević K, Nestorović B, Beskoski V. Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics. 2023;20(1):12-18.
doi:10.2174/1570164620666230220112500 .

Nikolić, Aleksandra, Mark, Kevin J., Dragičević, Sandra, Babić, Tamara, Milošević, Katarina, Nestorović, Branimir, Beskoski, Vladimir, "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma" in Current Proteomics, 20, no. 1 (2023):12-18,
https://doi.org/10.2174/1570164620666230220112500 . .

TY  - JOUR
AU  - Milošević, Katarina
AU  - Rsovac, Snežana
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2319
AB  - INTRODUCTION: Several studies have identified neuromuscular blocking agents as the most common cause of
anaphylaxis during general anesthesia. The reported data vary considerably between countries.
AIM: In this study we have investigated anaphylactic reactions to neuromuscular blocking agents, general
anaesthetics and latex in children from Serbia.
METHODS: This case series study has encompassed hypersensitivity testing in 78 children over a period of twelve
consecutive months. The diagnostic protocol consisted of a case history, immunoglobulin E (IgE) testing and skin
tests for hypersensitivity to neuromuscular blocking agents, general anesthetics and latex.
RESULTS: Of 78 children, 63% were hypersensitive to neuromuscular blocking agents, 28% to latex, 13% to
hypnotics, 10% to opioids and 15% to coloids. Sixty cases (77%) of anaphylaxis were IgE-mediated. Among
children who underwent more than one intervention, the presence of cross-sensibilisation for neuromuscular
blocking agents commercially available in Serbia was identified in 77% of cases.
CONCLUSION: Neuromuscular blocking agents were the most frequent causal agents of anaphylaxis in Serbian
children, as verified by skin tests and specific IgE tests. High frequencies of allergies to neuromuscular blocking
agents and latex obtained in this study underscore the importance of carrying out an allergy study to detect an
allergic cause in perioperative reactions
AB  - UVOD: Nekoliko studija je identifikovalo neuromuskularne blokatore kao najčešći uzrok anafilakse tokom opšte
anestezije, ali prijavljeni podaci znatno variraju između zemalja.
CILJ: U ovoj studiji ispitivane su anafilaktičke reakcije na neuromuskularne blokatore, opšte anestetike i lateks
kod dece iz Srbije.
METODE: Ova studija serije slučajeva obuhvatila je testiranje preosetljivosti kod 78 dece tokom perioda od
dvanaest uzastopnih meseci. Dijagnostički protokol se sastojao od istorije slučajeva, testiranja imunoglobulina E
(IgE) i kožnih testova za preosetljivost na neuromuskularne blokatore, opšte anestetike i lateks.
REZULTATI: Od 78 ispitivane dece, 63% je preosetljivo na neuromuskularne blokatore, 28% na lateks, 13% na
hipnotike, 10% na opioide i 15% na koloide. U 60 slučajeva (77%) anafilaksija je posredovana IgE-om. Među
decom koja su prošla više od jedne intervencije, prisustvo unakrsne senzibilizacije za neuromuskularne bokatore
komercijalno dostupne u Srbiji identifikovano je u 77% slučajeva.
ZAKLJUČAK: Neuromuskularni blokatori najčešći su uzročni agensi anafilaksije kod srpske dece, što je potvrđeno
testovima kože i specifičnim IgE testovima. Visoke učestalosti alergija na neuromuskularne blokatore i lateks
dobijene u ovoj studiji naglašavaju važnost sprovođenja studija alergije kako bi se otkrili alergijski uzroci u
perioperativnim reakcijama.
PB  - Novi Sad : Udruženje Medicina danas"
T2  - Medicina danas
T1  - Serbian study of perioperative anaphylaxis in children
T1  - Perioperativna anafilaksa kod dece u Srbiji
EP  - 180
IS  - 10-12
SP  - 175
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2319
ER  - 

@article{
author = "Milošević, Katarina and Rsovac, Snežana and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2018",
abstract = "INTRODUCTION: Several studies have identified neuromuscular blocking agents as the most common cause of
anaphylaxis during general anesthesia. The reported data vary considerably between countries.
AIM: In this study we have investigated anaphylactic reactions to neuromuscular blocking agents, general
anaesthetics and latex in children from Serbia.
METHODS: This case series study has encompassed hypersensitivity testing in 78 children over a period of twelve
consecutive months. The diagnostic protocol consisted of a case history, immunoglobulin E (IgE) testing and skin
tests for hypersensitivity to neuromuscular blocking agents, general anesthetics and latex.
RESULTS: Of 78 children, 63% were hypersensitive to neuromuscular blocking agents, 28% to latex, 13% to
hypnotics, 10% to opioids and 15% to coloids. Sixty cases (77%) of anaphylaxis were IgE-mediated. Among
children who underwent more than one intervention, the presence of cross-sensibilisation for neuromuscular
blocking agents commercially available in Serbia was identified in 77% of cases.
CONCLUSION: Neuromuscular blocking agents were the most frequent causal agents of anaphylaxis in Serbian
children, as verified by skin tests and specific IgE tests. High frequencies of allergies to neuromuscular blocking
agents and latex obtained in this study underscore the importance of carrying out an allergy study to detect an
allergic cause in perioperative reactions, UVOD: Nekoliko studija je identifikovalo neuromuskularne blokatore kao najčešći uzrok anafilakse tokom opšte
anestezije, ali prijavljeni podaci znatno variraju između zemalja.
CILJ: U ovoj studiji ispitivane su anafilaktičke reakcije na neuromuskularne blokatore, opšte anestetike i lateks
kod dece iz Srbije.
METODE: Ova studija serije slučajeva obuhvatila je testiranje preosetljivosti kod 78 dece tokom perioda od
dvanaest uzastopnih meseci. Dijagnostički protokol se sastojao od istorije slučajeva, testiranja imunoglobulina E
(IgE) i kožnih testova za preosetljivost na neuromuskularne blokatore, opšte anestetike i lateks.
REZULTATI: Od 78 ispitivane dece, 63% je preosetljivo na neuromuskularne blokatore, 28% na lateks, 13% na
hipnotike, 10% na opioide i 15% na koloide. U 60 slučajeva (77%) anafilaksija je posredovana IgE-om. Među
decom koja su prošla više od jedne intervencije, prisustvo unakrsne senzibilizacije za neuromuskularne bokatore
komercijalno dostupne u Srbiji identifikovano je u 77% slučajeva.
ZAKLJUČAK: Neuromuskularni blokatori najčešći su uzročni agensi anafilaksije kod srpske dece, što je potvrđeno
testovima kože i specifičnim IgE testovima. Visoke učestalosti alergija na neuromuskularne blokatore i lateks
dobijene u ovoj studiji naglašavaju važnost sprovođenja studija alergije kako bi se otkrili alergijski uzroci u
perioperativnim reakcijama.",
publisher = "Novi Sad : Udruženje Medicina danas"",
journal = "Medicina danas",
title = "Serbian study of perioperative anaphylaxis in children, Perioperativna anafilaksa kod dece u Srbiji",
pages = "180-175",
number = "10-12",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2319"
}

Milošević, K., Rsovac, S., Nestorović, B.,& Nikolić, A.. (2018). Serbian study of perioperative anaphylaxis in children. in Medicina danas
Novi Sad : Udruženje Medicina danas"., 17(10-12), 175-180.
https://hdl.handle.net/21.15107/rcub_imagine_2319

Milošević K, Rsovac S, Nestorović B, Nikolić A. Serbian study of perioperative anaphylaxis in children. in Medicina danas. 2018;17(10-12):175-180.
https://hdl.handle.net/21.15107/rcub_imagine_2319 .

Milošević, Katarina, Rsovac, Snežana, Nestorović, Branimir, Nikolić, Aleksandra, "Serbian study of perioperative anaphylaxis in children" in Medicina danas, 17, no. 10-12 (2018):175-180,
https://hdl.handle.net/21.15107/rcub_imagine_2319 .