TY  - CONF
AU  - Ljubičić, Jelena
AU  - Bogdanović, Aleksandar
AU  - Babić, Tamara
AU  - Despotović, Jovana
AU  - Dugalić, Vladimir
AU  - Nikolić, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2100
AB  - Background: Our previous study has identified variant rs745430558 in the SMAD4 gene promoter as potential biomarker for adenocarcinoma of the pancreas. The allele delTT (10T instead of 12T) was present in malignant pancreatic tissue with a prevalence of 88%. As analysis of cfDNA in liquid biopsy represents a noninvasive approach for the diagnosis and monitoring of malignancies, the aim of this study was to determine the presence of 12T and 10T alleles in the peripheral blood of patients with suspected pancreatic malignancy. Material and Methods: The study was performed using cell-free DNA (cfDNA) isolated from the serum of 15 patients with morphological alterations of the pancreas. The presence of 12T and 10T alleles was assessed by allele specific quantitative real-time PCR. Results: Of 15 analyzed samples, 13 were diagnosed with adenocarcinoma of the pancreas (AcP), 1 with neuroendocrine tumor (NET), and 1 with pancreatitis. The 10T allele was present in 84.7% of cases with AcP and also in the sample from the patient with NET. In patient with pancreatitis only the 12T allele was detected. Conclusion: Our research has shown that the results of liquid biopsy of patients with AcP are in agreement with tissue specimens analysis. Targeted detection of the rs745430558 10T variant in patients with suspected pancreatic malignancies could be a potential biomarker for diagnosis of AcP in the future.
PB  - Belgrade :  Serbian Association on for Cancer Research
C3  - 6th Congress of the Serbian Association for Cancer Research (SDIR)
T1  - Variant rs745430558 in the SMAD4 gene promoter as a biomarker for adenocarcinoma of the pancreas
EP  - 85
IS  - 1
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2100
ER  - 

@conference{
author = "Ljubičić, Jelena and Bogdanović, Aleksandar and Babić, Tamara and Despotović, Jovana and Dugalić, Vladimir and Nikolić, Aleksandra",
year = "2023",
abstract = "Background: Our previous study has identified variant rs745430558 in the SMAD4 gene promoter as potential biomarker for adenocarcinoma of the pancreas. The allele delTT (10T instead of 12T) was present in malignant pancreatic tissue with a prevalence of 88%. As analysis of cfDNA in liquid biopsy represents a noninvasive approach for the diagnosis and monitoring of malignancies, the aim of this study was to determine the presence of 12T and 10T alleles in the peripheral blood of patients with suspected pancreatic malignancy. Material and Methods: The study was performed using cell-free DNA (cfDNA) isolated from the serum of 15 patients with morphological alterations of the pancreas. The presence of 12T and 10T alleles was assessed by allele specific quantitative real-time PCR. Results: Of 15 analyzed samples, 13 were diagnosed with adenocarcinoma of the pancreas (AcP), 1 with neuroendocrine tumor (NET), and 1 with pancreatitis. The 10T allele was present in 84.7% of cases with AcP and also in the sample from the patient with NET. In patient with pancreatitis only the 12T allele was detected. Conclusion: Our research has shown that the results of liquid biopsy of patients with AcP are in agreement with tissue specimens analysis. Targeted detection of the rs745430558 10T variant in patients with suspected pancreatic malignancies could be a potential biomarker for diagnosis of AcP in the future.",
publisher = "Belgrade :  Serbian Association on for Cancer Research",
journal = "6th Congress of the Serbian Association for Cancer Research (SDIR)",
title = "Variant rs745430558 in the SMAD4 gene promoter as a biomarker for adenocarcinoma of the pancreas",
pages = "85-85",
number = "1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2100"
}

Ljubičić, J., Bogdanović, A., Babić, T., Despotović, J., Dugalić, V.,& Nikolić, A.. (2023). Variant rs745430558 in the SMAD4 gene promoter as a biomarker for adenocarcinoma of the pancreas. in 6th Congress of the Serbian Association for Cancer Research (SDIR)
Belgrade :  Serbian Association on for Cancer Research.(1), 85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2100

Ljubičić J, Bogdanović A, Babić T, Despotović J, Dugalić V, Nikolić A. Variant rs745430558 in the SMAD4 gene promoter as a biomarker for adenocarcinoma of the pancreas. in 6th Congress of the Serbian Association for Cancer Research (SDIR). 2023;(1):85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2100 .

Ljubičić, Jelena, Bogdanović, Aleksandar, Babić, Tamara, Despotović, Jovana, Dugalić, Vladimir, Nikolić, Aleksandra, "Variant rs745430558 in the SMAD4 gene promoter as a biomarker for adenocarcinoma of the pancreas" in 6th Congress of the Serbian Association for Cancer Research (SDIR), no. 1 (2023):85-85,
https://hdl.handle.net/21.15107/rcub_imagine_2100 .