TY  - CONF
AU  - Carević, Tamara
AU  - Novović, Katarina
AU  - Ivanov, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2115
AB  - Introduction: Differentsugars are commonly used in the diet, but little is understood about the various
effects of human health that they can affect. Hence, the impact of sugars and their substitutes used in
diet on the development of virulence in Pseudomonas aeruginosa PAO1 was investigated. Sugars (fructose, demerara, coconut sugar, and cane sugar) and sugar substitutes (erythritol and stevia) were selected. The genes from three P. aeruginosa QS networks (las - lasI, lasR; rhl - rhlI, rhlR; PQS - pqsA, mvfR)
were used for RT-qPCR analysis in order to investigate whether the expression of these genes changes.
In this work, the focusis on the expression of genesinvolved in QS and the ability to form biofilms(a type
of structured community of microorganisms that is attached to the surface and connected by an exopolysaccharide matrix), as well as determining minimal inhibitory concentration of antibiotics in presence of tested compounds.
Methods: Microdiltuion assay, Antibiofilm assay, RT- qPCR
Results: In the presence of tested sugars and theirsubstitutes, the minimum inhibitory concentration of
commercial antibiotics increased, as well as the percentages of biofilm formation (for instance, the percentage of biofilm formation is 171% in the presence of coconut sugar). Furthermore, exposure of P.
aeruginosa to tested compounds caused the greatest increase in expression of virulence associated with
the lasI and pvdF genes.
Conclusion: More awareness and research is needed to highlight the effectssugars can have on P. aeruginosa and to propose new strategies to reduce this negative aspect.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa
EP  - 48
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2115
ER  - 

@conference{
author = "Carević, Tamara and Novović, Katarina and Ivanov, Marija",
year = "2023",
abstract = "Introduction: Differentsugars are commonly used in the diet, but little is understood about the various
effects of human health that they can affect. Hence, the impact of sugars and their substitutes used in
diet on the development of virulence in Pseudomonas aeruginosa PAO1 was investigated. Sugars (fructose, demerara, coconut sugar, and cane sugar) and sugar substitutes (erythritol and stevia) were selected. The genes from three P. aeruginosa QS networks (las - lasI, lasR; rhl - rhlI, rhlR; PQS - pqsA, mvfR)
were used for RT-qPCR analysis in order to investigate whether the expression of these genes changes.
In this work, the focusis on the expression of genesinvolved in QS and the ability to form biofilms(a type
of structured community of microorganisms that is attached to the surface and connected by an exopolysaccharide matrix), as well as determining minimal inhibitory concentration of antibiotics in presence of tested compounds.
Methods: Microdiltuion assay, Antibiofilm assay, RT- qPCR
Results: In the presence of tested sugars and theirsubstitutes, the minimum inhibitory concentration of
commercial antibiotics increased, as well as the percentages of biofilm formation (for instance, the percentage of biofilm formation is 171% in the presence of coconut sugar). Furthermore, exposure of P.
aeruginosa to tested compounds caused the greatest increase in expression of virulence associated with
the lasI and pvdF genes.
Conclusion: More awareness and research is needed to highlight the effectssugars can have on P. aeruginosa and to propose new strategies to reduce this negative aspect.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2115"
}

Carević, T., Novović, K.,& Ivanov, M.. (2023). Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 48-48.
https://hdl.handle.net/21.15107/rcub_imagine_2115

Carević T, Novović K, Ivanov M. Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:48-48.
https://hdl.handle.net/21.15107/rcub_imagine_2115 .

Carević, Tamara, Novović, Katarina, Ivanov, Marija, "Sugars and their substitutes increase pathogenicity of Pseudomonas aeruginosa" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):48-48,
https://hdl.handle.net/21.15107/rcub_imagine_2115 .