TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Ines
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajcević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1574
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line
EP  - 399
SP  - 387
VL  - 21
DO  - 10.17179/excli2021-4487
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Ines and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajcević, Nemanja and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line",
pages = "399-387",
volume = "21",
doi = "10.17179/excli2021-4487"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajcević, N., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2022). Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajcević N, Stevanović M, Ferreira ICFR, Soković M. Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Ines, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajcević, Nemanja, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Bozunović, Jelena
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
AU  - Rajcević, Nemanja
AU  - Stojković, Dejan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1533
AB  - Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents
VL  - 175
DO  - 10.1016/j.indcrop.2021.114260
ER  - 

@article{
author = "Ivanov, Marija and Bozunović, Jelena and Gašić, Uroš and Drakulić, Danijela and Stevanović, Milena and Rajcević, Nemanja and Stojković, Dejan",
year = "2022",
abstract = "Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents",
volume = "175",
doi = "10.1016/j.indcrop.2021.114260"
}

Ivanov, M., Bozunović, J., Gašić, U., Drakulić, D., Stevanović, M., Rajcević, N.,& Stojković, D.. (2022). Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products
Elsevier, Amsterdam., 175.
https://doi.org/10.1016/j.indcrop.2021.114260

Ivanov M, Bozunović J, Gašić U, Drakulić D, Stevanović M, Rajcević N, Stojković D. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products. 2022;175.
doi:10.1016/j.indcrop.2021.114260 .

Ivanov, Marija, Bozunović, Jelena, Gašić, Uroš, Drakulić, Danijela, Stevanović, Milena, Rajcević, Nemanja, Stojković, Dejan, "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents" in Industrial Crops and Products, 175 (2022),
https://doi.org/10.1016/j.indcrop.2021.114260 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Rajcević, Nemanja
AU  - Stevanović, Milena
AU  - Soković, Marina D.
AU  - Gašić, Uroš
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1489
AB  - Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
IS  - 1
VL  - 14
DO  - 10.3390/ph14010055
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Schwirtlich, Marija and Rajcević, Nemanja and Stevanović, Milena and Soković, Marina D. and Gašić, Uroš",
year = "2021",
abstract = "Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions",
number = "1",
volume = "14",
doi = "10.3390/ph14010055"
}

Stojković, D., Drakulić, D., Schwirtlich, M., Rajcević, N., Stevanović, M., Soković, M. D.,& Gašić, U.. (2021). Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals
MDPI, Basel., 14(1).
https://doi.org/10.3390/ph14010055

Stojković D, Drakulić D, Schwirtlich M, Rajcević N, Stevanović M, Soković MD, Gašić U. Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals. 2021;14(1).
doi:10.3390/ph14010055 .

Stojković, Dejan, Drakulić, Danijela, Schwirtlich, Marija, Rajcević, Nemanja, Stevanović, Milena, Soković, Marina D., Gašić, Uroš, "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions" in Pharmaceuticals, 14, no. 1 (2021),
https://doi.org/10.3390/ph14010055 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajcević, Nemanja
AU  - Soković, Marina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1501
AB  - Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of a-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.
PB  - Elsevier, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.
VL  - 195
DO  - 10.1016/j.jpba.2020.113884
ER  - 

@article{
author = "Stojković, Dejan and Gašić, Uroš and Drakulić, Danijela and Zengin, Gokhan and Stevanović, Milena and Rajcević, Nemanja and Soković, Marina",
year = "2021",
abstract = "Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of a-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.",
volume = "195",
doi = "10.1016/j.jpba.2020.113884"
}

Stojković, D., Gašić, U., Drakulić, D., Zengin, G., Stevanović, M., Rajcević, N.,& Soković, M.. (2021). Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier, Amsterdam., 195.
https://doi.org/10.1016/j.jpba.2020.113884

Stojković D, Gašić U, Drakulić D, Zengin G, Stevanović M, Rajcević N, Soković M. Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis. 2021;195.
doi:10.1016/j.jpba.2020.113884 .

Stojković, Dejan, Gašić, Uroš, Drakulić, Danijela, Zengin, Gokhan, Stevanović, Milena, Rajcević, Nemanja, Soković, Marina, "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L." in Journal of Pharmaceutical and Biomedical Analysis, 195 (2021),
https://doi.org/10.1016/j.jpba.2020.113884 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajcević, Nemanja
AU  - Soković, Marina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1312
AB  - This study explored the chemical profile of the aerial parts ofOnonis spinosaand further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential againstStaphylococcus aureusand was able to supress the production of staphyloxanthin inS. aureusat sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract
EP  - 7151
IS  - 8
SP  - 7138
VL  - 11
DO  - 10.1039/d0fo01595d
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Gašić, Uroš and Zengin, Gokhan and Stevanović, Milena and Rajcević, Nemanja and Soković, Marina",
year = "2020",
abstract = "This study explored the chemical profile of the aerial parts ofOnonis spinosaand further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential againstStaphylococcus aureusand was able to supress the production of staphyloxanthin inS. aureusat sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract",
pages = "7151-7138",
number = "8",
volume = "11",
doi = "10.1039/d0fo01595d"
}

Stojković, D., Drakulić, D., Gašić, U., Zengin, G., Stevanović, M., Rajcević, N.,& Soković, M.. (2020). Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function
Royal Soc Chemistry, Cambridge., 11(8), 7138-7151.
https://doi.org/10.1039/d0fo01595d

Stojković D, Drakulić D, Gašić U, Zengin G, Stevanović M, Rajcević N, Soković M. Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function. 2020;11(8):7138-7151.
doi:10.1039/d0fo01595d .

Stojković, Dejan, Drakulić, Danijela, Gašić, Uroš, Zengin, Gokhan, Stevanović, Milena, Rajcević, Nemanja, Soković, Marina, "Ononis spinosaL., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract" in Food & Function, 11, no. 8 (2020):7138-7151,
https://doi.org/10.1039/d0fo01595d . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/835
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.
PB  - Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data
IS  - 5
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Sarac, Zorica and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.",
publisher = "Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data",
number = "5",
volume = "49",
doi = "10.14214/sf.1415"
}

Sarac, Z., Dodos, T., Rajcević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica
Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa., 49(5).
https://doi.org/10.14214/sf.1415

Sarac Z, Dodos T, Rajcević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica. 2015;49(5).
doi:10.14214/sf.1415 .

Sarac, Zorica, Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data" in Silva Fennica, 49, no. 5 (2015),
https://doi.org/10.14214/sf.1415 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksić, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/815
AB  - Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra
EP  - 217
IS  - 1
SP  - 205
VL  - 47
DO  - 10.2298/GENSR1501205S
ER  - 

@article{
author = "Sarac, Zorica and Aleksić, Jelena M. and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra",
pages = "217-205",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S"
}

Sarac, Z., Aleksić, J. M., Dodos, T., Rajcević, N., Bojović, S.,& Marin, P. D.. (2015). Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksić JM, Dodos T, Rajcević N, Bojović S, Marin PD. Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksić, Jelena M., Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .