TY  - JOUR
AU  - Jeran, Marko
AU  - Romolo, Anna
AU  - Spasovski, Vesna
AU  - Hočevar, Matej
AU  - Novak, Urban
AU  - Štukelj, Roman
AU  - Šuštar, Vid
AU  - Kisovec, Matic
AU  - Bedina Zavec, Apolonija
AU  - Kogej, Ksenija
AU  - Iglič, Aleš
AU  - Trebše, Polonca
AU  - Kralj-Iglič, Veronika
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2086
AB  - Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Small Cellular Particles from European Spruce Needle Homogenate
IS  - 5
SP  - 4349
VL  - 24
DO  - 10.3390/ijms24054349
ER  - 

@article{
author = "Jeran, Marko and Romolo, Anna and Spasovski, Vesna and Hočevar, Matej and Novak, Urban and Štukelj, Roman and Šuštar, Vid and Kisovec, Matic and Bedina Zavec, Apolonija and Kogej, Ksenija and Iglič, Aleš and Trebše, Polonca and Kralj-Iglič, Veronika",
year = "2023",
abstract = "Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Small Cellular Particles from European Spruce Needle Homogenate",
number = "5",
pages = "4349",
volume = "24",
doi = "10.3390/ijms24054349"
}

Jeran, M., Romolo, A., Spasovski, V., Hočevar, M., Novak, U., Štukelj, R., Šuštar, V., Kisovec, M., Bedina Zavec, A., Kogej, K., Iglič, A., Trebše, P.,& Kralj-Iglič, V.. (2023). Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences
MDPI., 24(5), 4349.
https://doi.org/10.3390/ijms24054349

Jeran M, Romolo A, Spasovski V, Hočevar M, Novak U, Štukelj R, Šuštar V, Kisovec M, Bedina Zavec A, Kogej K, Iglič A, Trebše P, Kralj-Iglič V. Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences. 2023;24(5):4349.
doi:10.3390/ijms24054349 .

Jeran, Marko, Romolo, Anna, Spasovski, Vesna, Hočevar, Matej, Novak, Urban, Štukelj, Roman, Šuštar, Vid, Kisovec, Matic, Bedina Zavec, Apolonija, Kogej, Ksenija, Iglič, Aleš, Trebše, Polonca, Kralj-Iglič, Veronika, "Small Cellular Particles from European Spruce Needle Homogenate" in International Journal of Molecular Sciences, 24, no. 5 (2023):4349,
https://doi.org/10.3390/ijms24054349 . .