TY  - JOUR
AU  - Schwirtlich, Marija
AU  - Kwakowsky, Andrea
AU  - Emri, Zsuzsa
AU  - Antal, Karoly
AU  - Lacza, Zsombor
AU  - Cselenyak, Attila
AU  - Katarova, Zoya
AU  - Szabo, Gabor
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/490
AB  - Primary lens epithelial cell (LEC) cultures derived from newborn (P0) and one-month-old (P30) mouse lenses were used to study GABA (gamma-aminobutyric acid) signaling expression and its effect on the intracellular Ca2+ ([Ca2+](i)) level. We have found that these cultures express specific cellular markers for lens epithelial and fiber cells, all components of the functional GABA signaling pathway and GABA, thus recapitulating the developmental program of the ocular lens. Activation of both GABA-A and GABA-B receptors (GABA(A)R and GABA(B)R) with the specific agonists muscimol and baclofen, respectively induces [Ca2+](i) transients that could be blocked by the specific antagonists bicuculline and CGP55845 and were dependent on extracellular Ca2+. Bicuculline did not change the GABA-evoked Ca2+ responses in Ca-2-containing buffers, but suppressed them significantly in Ca2+-free buffers suggesting the two receptors couple to convergent Ca2+ mobilization mechanisms with different extracellular Ca2+ sensitivity. Prolonged activation of GABA(B)R induced wave propagation of the Ca2+ signal and persistent oscillations. The number of cells reacting to GABA or GABA + bicuculline in P30 mouse LEC cultures expressing predominantly the synaptic type GABA(A)R did not differ significantly from the number of reacting cells in PO mouse LEC cultures. The GABA-induced Ca2+ transients in P30 (but not P0) mouse LEC could be entirely suppressed by co-application of bicuculline and CGP55845. The GABA-mediated Ca2+ signaling may be involved in a variety of Ca2+-dependent cellular processes during lens growth and epithelial cell differentiation.
PB  - Elsevier Sci Ltd, Oxford
T2  - Cell Calcium
T1  - GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation
EP  - 392
IS  - 4
SP  - 381
VL  - 50
DO  - 10.1016/j.ceca.2011.07.002
ER  - 

@article{
author = "Schwirtlich, Marija and Kwakowsky, Andrea and Emri, Zsuzsa and Antal, Karoly and Lacza, Zsombor and Cselenyak, Attila and Katarova, Zoya and Szabo, Gabor",
year = "2011",
abstract = "Primary lens epithelial cell (LEC) cultures derived from newborn (P0) and one-month-old (P30) mouse lenses were used to study GABA (gamma-aminobutyric acid) signaling expression and its effect on the intracellular Ca2+ ([Ca2+](i)) level. We have found that these cultures express specific cellular markers for lens epithelial and fiber cells, all components of the functional GABA signaling pathway and GABA, thus recapitulating the developmental program of the ocular lens. Activation of both GABA-A and GABA-B receptors (GABA(A)R and GABA(B)R) with the specific agonists muscimol and baclofen, respectively induces [Ca2+](i) transients that could be blocked by the specific antagonists bicuculline and CGP55845 and were dependent on extracellular Ca2+. Bicuculline did not change the GABA-evoked Ca2+ responses in Ca-2-containing buffers, but suppressed them significantly in Ca2+-free buffers suggesting the two receptors couple to convergent Ca2+ mobilization mechanisms with different extracellular Ca2+ sensitivity. Prolonged activation of GABA(B)R induced wave propagation of the Ca2+ signal and persistent oscillations. The number of cells reacting to GABA or GABA + bicuculline in P30 mouse LEC cultures expressing predominantly the synaptic type GABA(A)R did not differ significantly from the number of reacting cells in PO mouse LEC cultures. The GABA-induced Ca2+ transients in P30 (but not P0) mouse LEC could be entirely suppressed by co-application of bicuculline and CGP55845. The GABA-mediated Ca2+ signaling may be involved in a variety of Ca2+-dependent cellular processes during lens growth and epithelial cell differentiation.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Cell Calcium",
title = "GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation",
pages = "392-381",
number = "4",
volume = "50",
doi = "10.1016/j.ceca.2011.07.002"
}

Schwirtlich, M., Kwakowsky, A., Emri, Z., Antal, K., Lacza, Z., Cselenyak, A., Katarova, Z.,& Szabo, G.. (2011). GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation. in Cell Calcium
Elsevier Sci Ltd, Oxford., 50(4), 381-392.
https://doi.org/10.1016/j.ceca.2011.07.002

Schwirtlich M, Kwakowsky A, Emri Z, Antal K, Lacza Z, Cselenyak A, Katarova Z, Szabo G. GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation. in Cell Calcium. 2011;50(4):381-392.
doi:10.1016/j.ceca.2011.07.002 .

Schwirtlich, Marija, Kwakowsky, Andrea, Emri, Zsuzsa, Antal, Karoly, Lacza, Zsombor, Cselenyak, Attila, Katarova, Zoya, Szabo, Gabor, "GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation" in Cell Calcium, 50, no. 4 (2011):381-392,
https://doi.org/10.1016/j.ceca.2011.07.002 . .

TY  - JOUR
AU  - Schwirtlich, Marija
AU  - Emri, Zsuzsa
AU  - Antal, Karoly
AU  - Mate, Zoltan
AU  - Katarova, Zoya
AU  - Szabo, Gabor
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/420
AB  - Gamma-amminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system of vertebrates, serves as an autocrine/paracrine signaling molecule during development, modulating a number of calcium (Ca2+)-dependent processes, including proliferation, migration, and differentiation, acting via 2 types of GABA receptors (GABARs): ionotropic GABA(A)Rs and metabotropic GABA(B)Rs. Here, we demonstrate that mouse embryonic stem cells (mESCs), which possess the capacity for virtually unlimited self-renewal and pluripotency, synthesize GABA and express functional GABA(A)Rs and GABA(B)Rs, as well as voltage-gated calcium channels (VGCCs), ryanodine receptors (RyRs), and inwardly rectifying potassium (GIRK) channels. On activation, both GABAR types triggered synergistically intracellular calcium rise. Muscimol (a GABA(A)R agonist) induced single Ca2+ transients involving both VGCC-mediated Ca2+ influx and intracellular stores, while baclofen (a GABA(B)R agonist) evoked Ca2+ transients followed by intercellular Ca2+ waves and oscillations that were resistant to antagonists and entirely dependent on Ca2+ release from intracellular stores. Prolonged treatment with muscimol slightly inhibited, while baclofen or SR95531 (a GABA(A)R antagonist) significantly facilitated, mESC proliferation. GABA(A)R-specific ligands also induced morphological and gene expression changes indicating a differentiation shift. Our data suggest that the interplay between GABARs and downstream (coupled) effectors differentially modulates mESC proliferation/differentiation through selective activation of second messenger signaling cascades.-Schwirtlich, M., Emri, Z., Antal, K., Mate, Z., Katarova, Z., Szabo, G. GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+. FASEB J. 24, 1218-1228 (2010). www.fasebj.org
PB  - Federation Amer Soc Exp Biol, Bethesda
T2  - FASEB Journal
T1  - GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+
EP  - 1228
IS  - 4
SP  - 1218
VL  - 24
DO  - 10.1096/fj.09-143586
ER  - 

@article{
author = "Schwirtlich, Marija and Emri, Zsuzsa and Antal, Karoly and Mate, Zoltan and Katarova, Zoya and Szabo, Gabor",
year = "2010",
abstract = "Gamma-amminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system of vertebrates, serves as an autocrine/paracrine signaling molecule during development, modulating a number of calcium (Ca2+)-dependent processes, including proliferation, migration, and differentiation, acting via 2 types of GABA receptors (GABARs): ionotropic GABA(A)Rs and metabotropic GABA(B)Rs. Here, we demonstrate that mouse embryonic stem cells (mESCs), which possess the capacity for virtually unlimited self-renewal and pluripotency, synthesize GABA and express functional GABA(A)Rs and GABA(B)Rs, as well as voltage-gated calcium channels (VGCCs), ryanodine receptors (RyRs), and inwardly rectifying potassium (GIRK) channels. On activation, both GABAR types triggered synergistically intracellular calcium rise. Muscimol (a GABA(A)R agonist) induced single Ca2+ transients involving both VGCC-mediated Ca2+ influx and intracellular stores, while baclofen (a GABA(B)R agonist) evoked Ca2+ transients followed by intercellular Ca2+ waves and oscillations that were resistant to antagonists and entirely dependent on Ca2+ release from intracellular stores. Prolonged treatment with muscimol slightly inhibited, while baclofen or SR95531 (a GABA(A)R antagonist) significantly facilitated, mESC proliferation. GABA(A)R-specific ligands also induced morphological and gene expression changes indicating a differentiation shift. Our data suggest that the interplay between GABARs and downstream (coupled) effectors differentially modulates mESC proliferation/differentiation through selective activation of second messenger signaling cascades.-Schwirtlich, M., Emri, Z., Antal, K., Mate, Z., Katarova, Z., Szabo, G. GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+. FASEB J. 24, 1218-1228 (2010). www.fasebj.org",
publisher = "Federation Amer Soc Exp Biol, Bethesda",
journal = "FASEB Journal",
title = "GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+",
pages = "1228-1218",
number = "4",
volume = "24",
doi = "10.1096/fj.09-143586"
}

Schwirtlich, M., Emri, Z., Antal, K., Mate, Z., Katarova, Z.,& Szabo, G.. (2010). GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+. in FASEB Journal
Federation Amer Soc Exp Biol, Bethesda., 24(4), 1218-1228.
https://doi.org/10.1096/fj.09-143586

Schwirtlich M, Emri Z, Antal K, Mate Z, Katarova Z, Szabo G. GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+. in FASEB Journal. 2010;24(4):1218-1228.
doi:10.1096/fj.09-143586 .

Schwirtlich, Marija, Emri, Zsuzsa, Antal, Karoly, Mate, Zoltan, Katarova, Zoya, Szabo, Gabor, "GABA(A) and GABA(B) receptors of distinct properties affect oppositely the proliferation of mouse embryonic stem cells through synergistic elevation of intracellular Ca2+" in FASEB Journal, 24, no. 4 (2010):1218-1228,
https://doi.org/10.1096/fj.09-143586 . .