TY  - CONF
AU  - Trivanović, Drenka
AU  - Kovačević, Jovana
AU  - Arsić, Aleksandra
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Okić Djordjević, Ivana
AU  - Živanović, Milena
AU  - Mojsilović, Slavko
AU  - Maljković, Mirjana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1995
AB  - As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated
skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT).
Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT)
were obtained from matched osteoarthritis patients. Patients were classified into two
groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored
by using thin layer/gas chromatography coupled with cellular and molecular assays.
Data were interpreted and visualized by applying linear discriminant analysis (LDA) and
hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric
tests and Spearman’s rank correlation.
Analyses of total lipids revealed significantly reduced triglyceride content in femoral
(fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously
released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than
in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that
fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in
FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid
was increased in aged group in all AT depots, while stearic acid declined in aged group in
BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037).
Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells
was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot.
Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their
acetabular counterparts.
Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment
of stem cells within this skeletal site.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity
EP  - 54
SP  - 54
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1995
ER  - 

@conference{
author = "Trivanović, Drenka and Kovačević, Jovana and Arsić, Aleksandra and Vujačić, Marko and Bogosavljević, Nikola and Okić Djordjević, Ivana and Živanović, Milena and Mojsilović, Slavko and Maljković, Mirjana and Jauković, Aleksandra",
year = "2023",
abstract = "As adipose tissue (AT) undergoes metabolic reprogramming with age, we investigated
skeletal site-specific and age-dependent lipid profile of bone marrow adipose tissue (BMAT).
Acetabular and femoral BMAT, and gluteofemoral subcutaneous adipose tissue (gfSAT)
were obtained from matched osteoarthritis patients. Patients were classified into two
groups: younger (≤ 60 years) and aged (>60 years) adults. BMAT and gfSAT were explored
by using thin layer/gas chromatography coupled with cellular and molecular assays.
Data were interpreted and visualized by applying linear discriminant analysis (LDA) and
hierarchical clustering of fatty acid (FA) composition. Statistics was estimated by nonparametric
tests and Spearman’s rank correlation.
Analyses of total lipids revealed significantly reduced triglyceride content in femoral
(fBMAT) than in acetabular BMAT (aBMAT) and gfSAT. Frequencies of spontaneously
released saturated palmitic (C16:0) and stearic acids (C18:0) were higher in fBMAT than
in aBMAT and gfSAT (p=0.036 and p=0.046, n=8). Cluster heatmap and LDA showed that
fBMAT differed to acetabular and gfSAT, while acetabular and gfSAT were more similar in
FA profiles. FA profiles of AT depots varied with patient’s age. Contribution of palmitic acid
was increased in aged group in all AT depots, while stearic acid declined in aged group in
BMAT compartments only. fBMAT cellularity declined with age (r=-0.675, n=14, p=0.037).
Additionally, the presence of CD45-CD31-CD34+CD24+ adipogenic progenitor (stem) cells
was increased in fBMAT (0.46±0.03%) when compared to aBMAT (0.21±0.01%) depot.
Femoral mesenchymal stem cells displayed pronounced adipogenesis comparing to their
acetabular counterparts.
Our findings suggest that specific lipid profile of fBMAT imposes adipogenic commitment
of stem cells within this skeletal site.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity",
pages = "54-54",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1995"
}

Trivanović, D., Kovačević, J., Arsić, A., Vujačić, M., Bogosavljević, N., Okić Djordjević, I., Živanović, M., Mojsilović, S., Maljković, M.,& Jauković, A.. (2023). Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 54-54.
https://hdl.handle.net/21.15107/rcub_imagine_1995

Trivanović D, Kovačević J, Arsić A, Vujačić M, Bogosavljević N, Okić Djordjević I, Živanović M, Mojsilović S, Maljković M, Jauković A. Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity. in 4th Belgrade Bioinformatics Conference. 2023;4:54-54.
https://hdl.handle.net/21.15107/rcub_imagine_1995 .

Trivanović, Drenka, Kovačević, Jovana, Arsić, Aleksandra, Vujačić, Marko, Bogosavljević, Nikola, Okić Djordjević, Ivana, Živanović, Milena, Mojsilović, Slavko, Maljković, Mirjana, Jauković, Aleksandra, "Fatty Acid Data Analysis Unravels Skeletal Site and Age-Specific Features of Human Bone Marrow Adiposity" in 4th Belgrade Bioinformatics Conference, 4 (2023):54-54,
https://hdl.handle.net/21.15107/rcub_imagine_1995 .