TY  - JOUR
AU  - Dinić, Miroslav
AU  - Burgess, Jamie L.
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - Thaller, Seth R.
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Strahinić, Ivana
AU  - Tomić-Canić, Marjana
AU  - Pastar, Irena
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400054RR
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2424
AB  - Intracellular pathogens including Staphylococcus aureus contribute to the non-healing phenotype of chronic wounds. Lactobacilli, well known as beneficial bacteria, are also reported to modulate the immune system, yet their role in cutaneous immunity remains largely unknown. We explored the therapeutic potential of bacteria-free postbiotics, bioactive lysates of lactobacilli, to reduce intracellular S. aureus colonization and promote healing. Fourteen postbiotics derived from various lactobacilli species were screened, and Latilactobacillus curvatus BGMK2-41 was selected for further analysis based on the most efficient ability to reduce intracellular infection by S. aureus diabetic foot ulcer clinical isolate and S. aureus USA300. Treatment of both infected keratinocytes in vitro and infected human skin ex vivo with BGMK2-41 postbiotic cleared S. aureus. Keratinocytes treated in vitro with BGMK2-41 upregulated expression of antimicrobial response genes, of which DEFB4, ANG, and RNASE7 were also found upregulated in treated ex vivo human skin together with CAMP exclusively upregulated ex vivo. Furthermore, BGMK2-41 postbiotic treatment has a multifaceted impact on the wound healing process. Treatment of keratinocytes stimulated cell migration and the expression of tight junction proteins, while in ex vivo human skin BGMK2-41 increased expression of anti-inflammatory cytokine IL-10, promoted re-epithelialization, and restored the epidermal barrier via upregulation of tight junction proteins. Together, this provides a potential therapeutic approach for persistent intracellular S. aureus infections.
PB  - Wiley Periodicals LLC
T2  - The FASEB Journal
T1  - Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo
IS  - 14
SP  - e23801
VL  - 38
DO  - 10.1096/fj.202400054RR
ER  - 

@article{
author = "Dinić, Miroslav and Burgess, Jamie L. and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and Thaller, Seth R. and Gonzalez, Tammy and Golić, Nataša and Strahinić, Ivana and Tomić-Canić, Marjana and Pastar, Irena",
year = "2024",
abstract = "Intracellular pathogens including Staphylococcus aureus contribute to the non-healing phenotype of chronic wounds. Lactobacilli, well known as beneficial bacteria, are also reported to modulate the immune system, yet their role in cutaneous immunity remains largely unknown. We explored the therapeutic potential of bacteria-free postbiotics, bioactive lysates of lactobacilli, to reduce intracellular S. aureus colonization and promote healing. Fourteen postbiotics derived from various lactobacilli species were screened, and Latilactobacillus curvatus BGMK2-41 was selected for further analysis based on the most efficient ability to reduce intracellular infection by S. aureus diabetic foot ulcer clinical isolate and S. aureus USA300. Treatment of both infected keratinocytes in vitro and infected human skin ex vivo with BGMK2-41 postbiotic cleared S. aureus. Keratinocytes treated in vitro with BGMK2-41 upregulated expression of antimicrobial response genes, of which DEFB4, ANG, and RNASE7 were also found upregulated in treated ex vivo human skin together with CAMP exclusively upregulated ex vivo. Furthermore, BGMK2-41 postbiotic treatment has a multifaceted impact on the wound healing process. Treatment of keratinocytes stimulated cell migration and the expression of tight junction proteins, while in ex vivo human skin BGMK2-41 increased expression of anti-inflammatory cytokine IL-10, promoted re-epithelialization, and restored the epidermal barrier via upregulation of tight junction proteins. Together, this provides a potential therapeutic approach for persistent intracellular S. aureus infections.",
publisher = "Wiley Periodicals LLC",
journal = "The FASEB Journal",
title = "Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo",
number = "14",
pages = "e23801",
volume = "38",
doi = "10.1096/fj.202400054RR"
}

Dinić, M., Burgess, J. L., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., Thaller, S. R., Gonzalez, T., Golić, N., Strahinić, I., Tomić-Canić, M.,& Pastar, I.. (2024). Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo. in The FASEB Journal
Wiley Periodicals LLC., 38(14), e23801.
https://doi.org/10.1096/fj.202400054RR

Dinić M, Burgess JL, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, Thaller SR, Gonzalez T, Golić N, Strahinić I, Tomić-Canić M, Pastar I. Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo. in The FASEB Journal. 2024;38(14):e23801.
doi:10.1096/fj.202400054RR .

Dinić, Miroslav, Burgess, Jamie L., Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, Thaller, Seth R., Gonzalez, Tammy, Golić, Nataša, Strahinić, Ivana, Tomić-Canić, Marjana, Pastar, Irena, "Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo" in The FASEB Journal, 38, no. 14 (2024):e23801,
https://doi.org/10.1096/fj.202400054RR . .