TY  - JOUR
AU  - Balint, Vanda
AU  - Perić, Mina
AU  - Dačić, Sanja
AU  - Stanisavljević Ninković, Danijela
AU  - Marjanović, Jelena
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2340
AB  - Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.
PB  - MDPI
T2  - Biomedicines
T1  - The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes
IS  - 4
SP  - 796
VL  - 12
DO  - 10.3390/biomedicines12040796
ER  - 

@article{
author = "Balint, Vanda and Perić, Mina and Dačić, Sanja and Stanisavljević Ninković, Danijela and Marjanović, Jelena and Popović, Jelena and Stevanović, Milena and Lazić, Andrijana",
year = "2024",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.",
publisher = "MDPI",
journal = "Biomedicines",
title = "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes",
number = "4",
pages = "796",
volume = "12",
doi = "10.3390/biomedicines12040796"
}

Balint, V., Perić, M., Dačić, S., Stanisavljević Ninković, D., Marjanović, J., Popović, J., Stevanović, M.,& Lazić, A.. (2024). The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines
MDPI., 12(4), 796.
https://doi.org/10.3390/biomedicines12040796

Balint V, Perić M, Dačić S, Stanisavljević Ninković D, Marjanović J, Popović J, Stevanović M, Lazić A. The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines. 2024;12(4):796.
doi:10.3390/biomedicines12040796 .

Balint, Vanda, Perić, Mina, Dačić, Sanja, Stanisavljević Ninković, Danijela, Marjanović, Jelena, Popović, Jelena, Stevanović, Milena, Lazić, Andrijana, "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes" in Biomedicines, 12, no. 4 (2024):796,
https://doi.org/10.3390/biomedicines12040796 . .

TY  - CONF
AU  - Dinić, Miroslav
AU  - L. Burgess, Jamie
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - R. Thaller, Seth
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Tomić- Canić, Marjana
AU  - Strahinić, Ivana
AU  - Pastar, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2378
AB  - Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2378
ER  - 

@conference{
author = "Dinić, Miroslav and L. Burgess, Jamie and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and R. Thaller, Seth and Gonzalez, Tammy and Golić, Nataša and Tomić- Canić, Marjana and Strahinić, Ivana and Pastar, Irena",
year = "2024",
abstract = "Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2378"
}

Dinić, M., L. Burgess, J., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., R. Thaller, S., Gonzalez, T., Golić, N., Tomić- Canić, M., Strahinić, I.,& Pastar, I.. (2024). HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378

Dinić M, L. Burgess J, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, R. Thaller S, Gonzalez T, Golić N, Tomić- Canić M, Strahinić I, Pastar I. HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

Dinić, Miroslav, L. Burgess, Jamie, Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, R. Thaller, Seth, Gonzalez, Tammy, Golić, Nataša, Tomić- Canić, Marjana, Strahinić, Ivana, Pastar, Irena, "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):133-133,
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Burgess, Jamie L.
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - Thaller, Seth R.
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Strahinić, Ivana
AU  - Tomić-Canić, Marjana
AU  - Pastar, Irena
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400054RR
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2424
AB  - Intracellular pathogens including Staphylococcus aureus contribute to the non-healing phenotype of chronic wounds. Lactobacilli, well known as beneficial bacteria, are also reported to modulate the immune system, yet their role in cutaneous immunity remains largely unknown. We explored the therapeutic potential of bacteria-free postbiotics, bioactive lysates of lactobacilli, to reduce intracellular S. aureus colonization and promote healing. Fourteen postbiotics derived from various lactobacilli species were screened, and Latilactobacillus curvatus BGMK2-41 was selected for further analysis based on the most efficient ability to reduce intracellular infection by S. aureus diabetic foot ulcer clinical isolate and S. aureus USA300. Treatment of both infected keratinocytes in vitro and infected human skin ex vivo with BGMK2-41 postbiotic cleared S. aureus. Keratinocytes treated in vitro with BGMK2-41 upregulated expression of antimicrobial response genes, of which DEFB4, ANG, and RNASE7 were also found upregulated in treated ex vivo human skin together with CAMP exclusively upregulated ex vivo. Furthermore, BGMK2-41 postbiotic treatment has a multifaceted impact on the wound healing process. Treatment of keratinocytes stimulated cell migration and the expression of tight junction proteins, while in ex vivo human skin BGMK2-41 increased expression of anti-inflammatory cytokine IL-10, promoted re-epithelialization, and restored the epidermal barrier via upregulation of tight junction proteins. Together, this provides a potential therapeutic approach for persistent intracellular S. aureus infections.
PB  - Wiley Periodicals LLC
T2  - The FASEB Journal
T1  - Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo
IS  - 14
SP  - e23801
VL  - 38
DO  - 10.1096/fj.202400054RR
ER  - 

@article{
author = "Dinić, Miroslav and Burgess, Jamie L. and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and Thaller, Seth R. and Gonzalez, Tammy and Golić, Nataša and Strahinić, Ivana and Tomić-Canić, Marjana and Pastar, Irena",
year = "2024",
abstract = "Intracellular pathogens including Staphylococcus aureus contribute to the non-healing phenotype of chronic wounds. Lactobacilli, well known as beneficial bacteria, are also reported to modulate the immune system, yet their role in cutaneous immunity remains largely unknown. We explored the therapeutic potential of bacteria-free postbiotics, bioactive lysates of lactobacilli, to reduce intracellular S. aureus colonization and promote healing. Fourteen postbiotics derived from various lactobacilli species were screened, and Latilactobacillus curvatus BGMK2-41 was selected for further analysis based on the most efficient ability to reduce intracellular infection by S. aureus diabetic foot ulcer clinical isolate and S. aureus USA300. Treatment of both infected keratinocytes in vitro and infected human skin ex vivo with BGMK2-41 postbiotic cleared S. aureus. Keratinocytes treated in vitro with BGMK2-41 upregulated expression of antimicrobial response genes, of which DEFB4, ANG, and RNASE7 were also found upregulated in treated ex vivo human skin together with CAMP exclusively upregulated ex vivo. Furthermore, BGMK2-41 postbiotic treatment has a multifaceted impact on the wound healing process. Treatment of keratinocytes stimulated cell migration and the expression of tight junction proteins, while in ex vivo human skin BGMK2-41 increased expression of anti-inflammatory cytokine IL-10, promoted re-epithelialization, and restored the epidermal barrier via upregulation of tight junction proteins. Together, this provides a potential therapeutic approach for persistent intracellular S. aureus infections.",
publisher = "Wiley Periodicals LLC",
journal = "The FASEB Journal",
title = "Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo",
number = "14",
pages = "e23801",
volume = "38",
doi = "10.1096/fj.202400054RR"
}

Dinić, M., Burgess, J. L., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., Thaller, S. R., Gonzalez, T., Golić, N., Strahinić, I., Tomić-Canić, M.,& Pastar, I.. (2024). Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo. in The FASEB Journal
Wiley Periodicals LLC., 38(14), e23801.
https://doi.org/10.1096/fj.202400054RR

Dinić M, Burgess JL, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, Thaller SR, Gonzalez T, Golić N, Strahinić I, Tomić-Canić M, Pastar I. Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo. in The FASEB Journal. 2024;38(14):e23801.
doi:10.1096/fj.202400054RR .

Dinić, Miroslav, Burgess, Jamie L., Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, Thaller, Seth R., Gonzalez, Tammy, Golić, Nataša, Strahinić, Ivana, Tomić-Canić, Marjana, Pastar, Irena, "Postbiotic lactobacilli induce cutaneous antimicrobial response and restore the barrier to inhibit the intracellular invasion of Staphylococcus aureus in vitro and ex vivo" in The FASEB Journal, 38, no. 14 (2024):e23801,
https://doi.org/10.1096/fj.202400054RR . .

TY  - CONF
AU  - Marjanović, Jelena
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1550
PB  - Springernature, London
C3  - European Journal of Human Genetics
T1  - SOX3 function in glioblastoma cells
EP  - 429
IS  - SUPPL 1
SP  - 428
VL  - 30
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1550
ER  - 

@conference{
author = "Marjanović, Jelena and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Garros-Regulez, Laura and Sampron, Nicolas and Matheu, Ander and Stevanović, Milena",
year = "2022",
publisher = "Springernature, London",
journal = "European Journal of Human Genetics",
title = "SOX3 function in glioblastoma cells",
pages = "429-428",
number = "SUPPL 1",
volume = "30",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1550"
}

Marjanović, J., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Garros-Regulez, L., Sampron, N., Matheu, A.,& Stevanović, M.. (2022). SOX3 function in glioblastoma cells. in European Journal of Human Genetics
Springernature, London., 30(SUPPL 1), 428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550

Marjanović J, Drakulić D, Garcia I, Vuković V, Aldaz P, Garros-Regulez L, Sampron N, Matheu A, Stevanović M. SOX3 function in glioblastoma cells. in European Journal of Human Genetics. 2022;30(SUPPL 1):428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

Marjanović, Jelena, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Garros-Regulez, Laura, Sampron, Nicolas, Matheu, Ander, Stevanović, Milena, "SOX3 function in glioblastoma cells" in European Journal of Human Genetics, 30, no. SUPPL 1 (2022):428-429,
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

TY  - THES
AU  - Marjanović, Jelena
PY  - 2019-06-28
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7077
UR  - https://nardus.mpn.gov.rs/handle/123456789/11740
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20712/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025220274
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2425
AB  - Glioblastom, glioma tumor gradusa IV, je najčešći maligni tumor mozga kododraslih i jedan od najsmrtonosnijih tipova tumora. I pored agresivne terapije koja obuhvatahirurško uklanjanje tumora, radio- i hemio-terapiju, prosečno preživljavanje bolesnika saovim tipom tumora je oko 15 meseci. Glioblastom (GBM), pored tumorskih ćelija, sadrži ipopulaciju samo-obnavljajućih tumor-inicirajućih matičnih ćelija (matične ćelijeglioblastoma) koje se smatraju odgovornim za nastanak, progresiju, metastaziranje irezistenciju na terapiju.Geni SOXB1 podgrupe (SOX1, SOX2 i SOX3) kodiraju regulatorne proteine kojiimaju značajne uloge u mnogim procesima u toku razvića, kao što su održavanjepluripotentnosti matičnih ćelija i održavanje populacije neuralnih progenitora upluripotentnom i proliferišućem stanju. Ovi geni imaju i značajne funkcije u procesukarcinogeneze. Ekspresija gena ove podgrupe detektovana je u GBM. Funkcija gena SOX2je dobro proučena kod ovog tipa tumora; pokazano je da ovaj gen promoviše malignipotencijal ćelija GBM i neophodan je za održavanje tumorogenog potencijala matičnihćelija glioblastoma. Za razliku od gena SOX2, uloga gena SOX1 i SOX3 u ćelijama GBMjoš uvek nije dovoljno istražena. Stoga, u okviru ove doktorske disertacije analizirana jeuloga ovih gena u ćelijama glioblastoma.Dobijeni rezultati pokazuju da ćelijske linije GBM eksprimiraju gen SOX1. Poredtoga, u uslovima utišane ekspresije proteina SOX1 detektovano je smanjenjeproliferativnog kapaciteta, vijabilnosti i migratornog potencijala U251 ćelija GBM, kao ipovećanje broja ovih ćelija u senescenciji. Nakon dediferencijacije ćelijskih linija GBMdetektovano je povećanje ekspresije gena SOX1 u poređenju sa ekspresijom uočenom unjihovim parentalnim ćelijama. Nivo ekspresije gena SOX1 povećan je u kulturamamatičnih ćelija glioblastoma u poređenju sa ekspresijom ovog gena u imortalizovanim U87i U251 ćelijama; pri diferencijaciji ovih kultura uočeno je smanjenje ekspresije gena SOX1.Utišavanje ekspresije gena SOX1 u GNS166 kulturi matičnih ćelija GBM dovodi dosmanjenja proliferativnog kapaciteta i vijabilnosti ovih ćelija...
AB  - one of deadliest cancers. Despite aggressive treatment, including surgical resection,chemotherapy and radiation, the median survival of patients with glioblastoma is 15months. A growing body of evidence indicates that GBM contains a population of selfrenewingtumor-initiating cells (glioblastoma stem cells - GSCs) that drive tumor initiation,propagation, metastasis and therapy resistance.SOXB1 genes (SOX1, SOX2 and SOX3) encode transcription regulators withimportant roles in embryonic development and carcinogenesis. Literature date revealed thatSOXB1 genes are expressed in GMB tumor samples. The role of one member of this group,SOX2 gene, is well documented in GBM. It was shown that SOX2 gene promotes malignantpotential of GBM cells and it is mandatory for maintenance of tumorogenicity of GSCs.Since the function of SOX1 and SOX3 genes in GBM still remains to be established, theaim of this thesis was to analyze the role of these genes in GBM.Obtained results demonstrated that all analyzed GBM cell lines express SOX1.Downregulation of this gene expression decreases proliferation, viability and migration,and induces senescence of U251 cells. Furthermore, dedifferentiation of GBM cells isaccompanied by increase of SOX1 level compared to that in parental cells. Expression ofthis gene was significantly increased in patient-derived GSC cultures compared to that inU87 and U251 cells; downregulation of SOX1 gene expression was seen upondifferentiation of GSCs. In addition, knock-down of this gene expression reducedproliferation and viability of GSCs.Results obtained in this thesis reveal SOX3 expression in GBM cell lines and tumortissue; the expression of this gene was elevated in the most of analyzed GBM samplescompared to expression levels detected in non-tumoral brain tissues. A high SOX3expression was not associated with the overall survival of GBM patients. Ectopicoverexpression of this gene increased proliferation, viability, migration and invasion ofGBM cells...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11740
ER  - 

@phdthesis{
author = "Marjanović, Jelena",
year = "2019-06-28",
abstract = "Glioblastom, glioma tumor gradusa IV, je najčešći maligni tumor mozga kododraslih i jedan od najsmrtonosnijih tipova tumora. I pored agresivne terapije koja obuhvatahirurško uklanjanje tumora, radio- i hemio-terapiju, prosečno preživljavanje bolesnika saovim tipom tumora je oko 15 meseci. Glioblastom (GBM), pored tumorskih ćelija, sadrži ipopulaciju samo-obnavljajućih tumor-inicirajućih matičnih ćelija (matične ćelijeglioblastoma) koje se smatraju odgovornim za nastanak, progresiju, metastaziranje irezistenciju na terapiju.Geni SOXB1 podgrupe (SOX1, SOX2 i SOX3) kodiraju regulatorne proteine kojiimaju značajne uloge u mnogim procesima u toku razvića, kao što su održavanjepluripotentnosti matičnih ćelija i održavanje populacije neuralnih progenitora upluripotentnom i proliferišućem stanju. Ovi geni imaju i značajne funkcije u procesukarcinogeneze. Ekspresija gena ove podgrupe detektovana je u GBM. Funkcija gena SOX2je dobro proučena kod ovog tipa tumora; pokazano je da ovaj gen promoviše malignipotencijal ćelija GBM i neophodan je za održavanje tumorogenog potencijala matičnihćelija glioblastoma. Za razliku od gena SOX2, uloga gena SOX1 i SOX3 u ćelijama GBMjoš uvek nije dovoljno istražena. Stoga, u okviru ove doktorske disertacije analizirana jeuloga ovih gena u ćelijama glioblastoma.Dobijeni rezultati pokazuju da ćelijske linije GBM eksprimiraju gen SOX1. Poredtoga, u uslovima utišane ekspresije proteina SOX1 detektovano je smanjenjeproliferativnog kapaciteta, vijabilnosti i migratornog potencijala U251 ćelija GBM, kao ipovećanje broja ovih ćelija u senescenciji. Nakon dediferencijacije ćelijskih linija GBMdetektovano je povećanje ekspresije gena SOX1 u poređenju sa ekspresijom uočenom unjihovim parentalnim ćelijama. Nivo ekspresije gena SOX1 povećan je u kulturamamatičnih ćelija glioblastoma u poređenju sa ekspresijom ovog gena u imortalizovanim U87i U251 ćelijama; pri diferencijaciji ovih kultura uočeno je smanjenje ekspresije gena SOX1.Utišavanje ekspresije gena SOX1 u GNS166 kulturi matičnih ćelija GBM dovodi dosmanjenja proliferativnog kapaciteta i vijabilnosti ovih ćelija..., one of deadliest cancers. Despite aggressive treatment, including surgical resection,chemotherapy and radiation, the median survival of patients with glioblastoma is 15months. A growing body of evidence indicates that GBM contains a population of selfrenewingtumor-initiating cells (glioblastoma stem cells - GSCs) that drive tumor initiation,propagation, metastasis and therapy resistance.SOXB1 genes (SOX1, SOX2 and SOX3) encode transcription regulators withimportant roles in embryonic development and carcinogenesis. Literature date revealed thatSOXB1 genes are expressed in GMB tumor samples. The role of one member of this group,SOX2 gene, is well documented in GBM. It was shown that SOX2 gene promotes malignantpotential of GBM cells and it is mandatory for maintenance of tumorogenicity of GSCs.Since the function of SOX1 and SOX3 genes in GBM still remains to be established, theaim of this thesis was to analyze the role of these genes in GBM.Obtained results demonstrated that all analyzed GBM cell lines express SOX1.Downregulation of this gene expression decreases proliferation, viability and migration,and induces senescence of U251 cells. Furthermore, dedifferentiation of GBM cells isaccompanied by increase of SOX1 level compared to that in parental cells. Expression ofthis gene was significantly increased in patient-derived GSC cultures compared to that inU87 and U251 cells; downregulation of SOX1 gene expression was seen upondifferentiation of GSCs. In addition, knock-down of this gene expression reducedproliferation and viability of GSCs.Results obtained in this thesis reveal SOX3 expression in GBM cell lines and tumortissue; the expression of this gene was elevated in the most of analyzed GBM samplescompared to expression levels detected in non-tumoral brain tissues. A high SOX3expression was not associated with the overall survival of GBM patients. Ectopicoverexpression of this gene increased proliferation, viability, migration and invasion ofGBM cells...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11740"
}

Marjanović, J.. (2019-06-28). Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_11740

Marjanović J. Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11740 .

Marjanović, Jelena, "Analiza uloge gena SOX1 i SOX3 u promovisanju malignog fenotipa ćelija glioblastoma" in Универзитет у Београду (2019-06-28),
https://hdl.handle.net/21.15107/rcub_nardus_11740 .

TY  - JOUR
AU  - Popović, Jelena
AU  - Stanisavljević Ninković, Danijela
AU  - Schwirtlich, Marija
AU  - Lazić, Andrijana
AU  - Marjanović, Jelena
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/785
AB  - SOX14 is a member of the SOXB2 subgroup of transcription factors implicated in neural development. Although the first SOX14 gene in vertebrates was cloned and characterized more than a decade ago and its expression profile during development was revealed in various animal model systems, the role of this gene during neural development is largely unknown. In the present study we analyzed the expression of SOX14 in human NT2/D1 and mouse P19 pluripotent embryonal carcinoma cells. We demonstrated that it is expressed in both cell lines and upregulated during retinoic acid induced neural differentiation. We showed that SOX14 was expressed in both neuronal and non-neuronal differentiated derivatives, as revealed by immunocytochemistry. Since it was previously proposed that increased SOXB2 proteins level interfere with the activity of SOXB1 counteracting partners, we compared expression patterns of SOXB members during retinoic acid induction of embryonal carcinoma cells. We revealed that upregulation of SOX14 expression is accompanied by alterations in the expression patterns of SOXB1 members. In order to analyze the potential cross-talk between them, we generated SOX14 expression construct. The ectopic expression of SOX14 was demonstrated at the mRNA level in NT2/D1, P19 and HeLa cells, while an increased level of SOX14 protein was detected in HeLa cells only. By transient transfection experiments in HeLa cells we showed for the first time that ectopic expression of SOX14 repressed SOX1 expression, whereas no significant effect on SOX2, SOX3 and SOX21 was observed. Data presented here provide an insight into SOX14 expression during in vitro neural differentiation of embryonal carcinoma cells and demonstrate the effect of its ectopic expression on protein levels of SOXB members in HeLa cells. Obtained results contribute to better understanding the role of one of the most conserved SOX proteins.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells
IS  - 3
VL  - 9
DO  - 10.1371/journal.pone.0091852
ER  - 

@article{
author = "Popović, Jelena and Stanisavljević Ninković, Danijela and Schwirtlich, Marija and Lazić, Andrijana and Marjanović, Jelena and Stevanović, Milena",
year = "2014",
abstract = "SOX14 is a member of the SOXB2 subgroup of transcription factors implicated in neural development. Although the first SOX14 gene in vertebrates was cloned and characterized more than a decade ago and its expression profile during development was revealed in various animal model systems, the role of this gene during neural development is largely unknown. In the present study we analyzed the expression of SOX14 in human NT2/D1 and mouse P19 pluripotent embryonal carcinoma cells. We demonstrated that it is expressed in both cell lines and upregulated during retinoic acid induced neural differentiation. We showed that SOX14 was expressed in both neuronal and non-neuronal differentiated derivatives, as revealed by immunocytochemistry. Since it was previously proposed that increased SOXB2 proteins level interfere with the activity of SOXB1 counteracting partners, we compared expression patterns of SOXB members during retinoic acid induction of embryonal carcinoma cells. We revealed that upregulation of SOX14 expression is accompanied by alterations in the expression patterns of SOXB1 members. In order to analyze the potential cross-talk between them, we generated SOX14 expression construct. The ectopic expression of SOX14 was demonstrated at the mRNA level in NT2/D1, P19 and HeLa cells, while an increased level of SOX14 protein was detected in HeLa cells only. By transient transfection experiments in HeLa cells we showed for the first time that ectopic expression of SOX14 repressed SOX1 expression, whereas no significant effect on SOX2, SOX3 and SOX21 was observed. Data presented here provide an insight into SOX14 expression during in vitro neural differentiation of embryonal carcinoma cells and demonstrate the effect of its ectopic expression on protein levels of SOXB members in HeLa cells. Obtained results contribute to better understanding the role of one of the most conserved SOX proteins.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells",
number = "3",
volume = "9",
doi = "10.1371/journal.pone.0091852"
}

Popović, J., Stanisavljević Ninković, D., Schwirtlich, M., Lazić, A., Marjanović, J.,& Stevanović, M.. (2014). Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells. in PLoS One
Public Library Science, San Francisco., 9(3).
https://doi.org/10.1371/journal.pone.0091852

Popović J, Stanisavljević Ninković D, Schwirtlich M, Lazić A, Marjanović J, Stevanović M. Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells. in PLoS One. 2014;9(3).
doi:10.1371/journal.pone.0091852 .

Popović, Jelena, Stanisavljević Ninković, Danijela, Schwirtlich, Marija, Lazić, Andrijana, Marjanović, Jelena, Stevanović, Milena, "Expression Analysis of SOX14 during Retinoic Acid Induced Neural Differentiation of Embryonal Carcinoma Cells and Assessment of the Effect of Its Ectopic Expression on SOXB Members in HeLa Cells" in PLoS One, 9, no. 3 (2014),
https://doi.org/10.1371/journal.pone.0091852 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Topalović, Vladanka
AU  - Marjanović, Jelena
AU  - Stevanović, Milena
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/682
AB  - Wnt signaling functions in numerous cellular activities such as cell fate determination, patterning, and migration in embryogenesis, apoptosis, etc. In this study, we used quercetin and lithium chloride to investigate modulations of the Wnt signaling pathway in human pluripotent embryonal carcinoma NT2/D1 cell line. First, we optimized conditions for NT2/D1 cell treatments with quercetin and lithium chloride and assessed their cytotoxic effects on the cells, cell viability and proliferation rate. Our results showed that induction of cell death by quercetin and LiCl is p53-dependent in NT2/D cells. We also examined the degree of Wnt signaling modulations by analyzing the expression of c-myc, a wellknown Wnt signaling target gene. Since the retinoic acid induction of NT2/D1 cells is good in an in vitro model system for human neural differentiation, studying Wnt signaling modulation in NT2/D1 would contribute to a better understanding of the mechanisms involved in neural stem cell maintenance and human neural development.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells
EP  - 209
IS  - 1
SP  - 201
VL  - 65
DO  - 10.2298/ABS1301201M
ER  - 

@article{
author = "Mojsin, Marija and Topalović, Vladanka and Marjanović, Jelena and Stevanović, Milena",
year = "2013",
abstract = "Wnt signaling functions in numerous cellular activities such as cell fate determination, patterning, and migration in embryogenesis, apoptosis, etc. In this study, we used quercetin and lithium chloride to investigate modulations of the Wnt signaling pathway in human pluripotent embryonal carcinoma NT2/D1 cell line. First, we optimized conditions for NT2/D1 cell treatments with quercetin and lithium chloride and assessed their cytotoxic effects on the cells, cell viability and proliferation rate. Our results showed that induction of cell death by quercetin and LiCl is p53-dependent in NT2/D cells. We also examined the degree of Wnt signaling modulations by analyzing the expression of c-myc, a wellknown Wnt signaling target gene. Since the retinoic acid induction of NT2/D1 cells is good in an in vitro model system for human neural differentiation, studying Wnt signaling modulation in NT2/D1 would contribute to a better understanding of the mechanisms involved in neural stem cell maintenance and human neural development.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells",
pages = "209-201",
number = "1",
volume = "65",
doi = "10.2298/ABS1301201M"
}

Mojsin, M., Topalović, V., Marjanović, J.,& Stevanović, M.. (2013). Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(1), 201-209.
https://doi.org/10.2298/ABS1301201M

Mojsin M, Topalović V, Marjanović J, Stevanović M. Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells. in Archives of Biological Sciences. 2013;65(1):201-209.
doi:10.2298/ABS1301201M .

Mojsin, Marija, Topalović, Vladanka, Marjanović, Jelena, Stevanović, Milena, "Quercetin and lithium chloride modulate Wnt signaling in pluripotent embryonal carcinoma NT2/D1 cells" in Archives of Biological Sciences, 65, no. 1 (2013):201-209,
https://doi.org/10.2298/ABS1301201M . .