TY  - JOUR
AU  - Pekmezović, Marina
AU  - Krusić, Melina Kalagasidis
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1440
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Krusić, Melina Kalagasidis and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Krusić, M. K., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel
MDPI, Basel., 10(6).
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Krusić MK, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel. 2021;10(6).
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Krusić, Melina Kalagasidis, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics-Basel, 10, no. 6 (2021),
https://doi.org/10.3390/antibiotics10060737 . .