TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Banović Đeri, Bojana
AU  - Miljuš-Đukić, Jovanka
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Cupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Spanu, Ilaria
AU  - Jelić, Mihailo
AU  - Maksimović, Vesna R.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/834
AB  - Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Genetics and Plant Breeding
T1  - A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
EP  - 39
IS  - 1
SP  - 36
VL  - 51
DO  - 10.17221/153/2014-CJGPB
ER  - 

@article{
author = "Aleksić, Jelena M. and Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Jovanović, Živko and Mikić, Aleksandar and Cupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Spanu, Ilaria and Jelić, Mihailo and Maksimović, Vesna R.",
year = "2015",
abstract = "Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Genetics and Plant Breeding",
title = "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba",
pages = "39-36",
number = "1",
volume = "51",
doi = "10.17221/153/2014-CJGPB"
}

Aleksić, J. M., Banović Đeri, B., Miljuš-Đukić, J., Jovanović, Ž., Mikić, A., Cupina, B., Zlatković, B., Anđelković, S., Spanu, I., Jelić, M.,& Maksimović, V. R.. (2015). A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding
Czech Academy Agricultural Sciences, Prague., 51(1), 36-39.
https://doi.org/10.17221/153/2014-CJGPB

Aleksić JM, Banović Đeri B, Miljuš-Đukić J, Jovanović Ž, Mikić A, Cupina B, Zlatković B, Anđelković S, Spanu I, Jelić M, Maksimović VR. A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding. 2015;51(1):36-39.
doi:10.17221/153/2014-CJGPB .

Aleksić, Jelena M., Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Jovanović, Živko, Mikić, Aleksandar, Cupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Spanu, Ilaria, Jelić, Mihailo, Maksimović, Vesna R., "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba" in Czech Journal of Genetics and Plant Breeding, 51, no. 1 (2015):36-39,
https://doi.org/10.17221/153/2014-CJGPB . .