TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Banović Đeri, Bojana
AU  - Miljuš-Đukić, Jovanka
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Cupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Spanu, Ilaria
AU  - Jelić, Mihailo
AU  - Maksimović, Vesna R.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/834
AB  - Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Genetics and Plant Breeding
T1  - A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
EP  - 39
IS  - 1
SP  - 36
VL  - 51
DO  - 10.17221/153/2014-CJGPB
ER  - 

@article{
author = "Aleksić, Jelena M. and Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Jovanović, Živko and Mikić, Aleksandar and Cupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Spanu, Ilaria and Jelić, Mihailo and Maksimović, Vesna R.",
year = "2015",
abstract = "Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Genetics and Plant Breeding",
title = "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba",
pages = "39-36",
number = "1",
volume = "51",
doi = "10.17221/153/2014-CJGPB"
}

Aleksić, J. M., Banović Đeri, B., Miljuš-Đukić, J., Jovanović, Ž., Mikić, A., Cupina, B., Zlatković, B., Anđelković, S., Spanu, I., Jelić, M.,& Maksimović, V. R.. (2015). A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding
Czech Academy Agricultural Sciences, Prague., 51(1), 36-39.
https://doi.org/10.17221/153/2014-CJGPB

Aleksić JM, Banović Đeri B, Miljuš-Đukić J, Jovanović Ž, Mikić A, Cupina B, Zlatković B, Anđelković S, Spanu I, Jelić M, Maksimović VR. A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding. 2015;51(1):36-39.
doi:10.17221/153/2014-CJGPB .

Aleksić, Jelena M., Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Jovanović, Živko, Mikić, Aleksandar, Cupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Spanu, Ilaria, Jelić, Mihailo, Maksimović, Vesna R., "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba" in Czech Journal of Genetics and Plant Breeding, 51, no. 1 (2015):36-39,
https://doi.org/10.17221/153/2014-CJGPB . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/812
AB  - Bitter vetch (Vicia ervilia (L.) Willd.) was a part of the everyday diet of the Eurasian Neanderthal population and the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. The major criteria to determine the domestication in bitter vetch and other ancient grain legumes are non-dehiscent pods, larger seed size and smooth seed testa. Bitter vetch seeds were found among the earliest findings of cultivated crops at the site of Tell El-Kerkh, Syria, from 10th millennium BP. Along with cereals, pea and lentil, bitter vetch has become definitely associated with the start of the 'agricultural revolution' in the Old World. Bitter vetch entered Europe in its south-east regions and progressed into its interior via Danube. Its distribution was rapid, since the available evidence reveals its presence in remote places at similar periods. Recently the first success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The linguistic evidence supports the fact that most of Eurasian peoples have their own words denoting bitter vetch, meaning that its cultivation preceded the diversification of their own proto-languages.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe
EP  - 11
IS  - 1
SP  - 1
VL  - 47
DO  - 10.2298/GENSR1501001M
ER  - 

@article{
author = "Mikić, Aleksandar and Medović, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja",
year = "2015",
abstract = "Bitter vetch (Vicia ervilia (L.) Willd.) was a part of the everyday diet of the Eurasian Neanderthal population and the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. The major criteria to determine the domestication in bitter vetch and other ancient grain legumes are non-dehiscent pods, larger seed size and smooth seed testa. Bitter vetch seeds were found among the earliest findings of cultivated crops at the site of Tell El-Kerkh, Syria, from 10th millennium BP. Along with cereals, pea and lentil, bitter vetch has become definitely associated with the start of the 'agricultural revolution' in the Old World. Bitter vetch entered Europe in its south-east regions and progressed into its interior via Danube. Its distribution was rapid, since the available evidence reveals its presence in remote places at similar periods. Recently the first success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The linguistic evidence supports the fact that most of Eurasian peoples have their own words denoting bitter vetch, meaning that its cultivation preceded the diversification of their own proto-languages.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe",
pages = "11-1",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501001M"
}

Mikić, A., Medović, A., Jovanović, Ž.,& Stanisavljević, N.. (2015). A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(1), 1-11.
https://doi.org/10.2298/GENSR1501001M

Mikić A, Medović A, Jovanović Ž, Stanisavljević N. A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe. in Genetika-Belgrade. 2015;47(1):1-11.
doi:10.2298/GENSR1501001M .

Mikić, Aleksandar, Medović, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, "A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe" in Genetika-Belgrade, 47, no. 1 (2015):1-11,
https://doi.org/10.2298/GENSR1501001M . .

TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Cupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/755
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 

@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Cupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}

Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Cupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z

Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Cupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .

Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Cupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources and Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/725
AB  - This short communication intends to discuss various models and ideas about the very beginnings of plant domestication in the Old World with a specific emphasis on pea (Pisum sativum L.) as one of the most significant crops in the human civilization. It presents a selection of some recent achievements and challenges in studying the earliest days of this important grain legume species by balanced contributions from archaeobotany, paleogenetics and historical linguistics. It will hopefully stimulate a fruitful interdisciplinary discussion and provide a basis for new experimental approaches.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)
EP  - 892
IS  - 5
SP  - 887
VL  - 61
DO  - 10.1007/s10722-014-0102-9
ER  - 

@article{
author = "Mikić, Aleksandar and Medović, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja",
year = "2014",
abstract = "This short communication intends to discuss various models and ideas about the very beginnings of plant domestication in the Old World with a specific emphasis on pea (Pisum sativum L.) as one of the most significant crops in the human civilization. It presents a selection of some recent achievements and challenges in studying the earliest days of this important grain legume species by balanced contributions from archaeobotany, paleogenetics and historical linguistics. It will hopefully stimulate a fruitful interdisciplinary discussion and provide a basis for new experimental approaches.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)",
pages = "892-887",
number = "5",
volume = "61",
doi = "10.1007/s10722-014-0102-9"
}

Mikić, A., Medović, A., Jovanović, Ž.,& Stanisavljević, N.. (2014). Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum). in Genetic Resources and Crop Evolution
Springer, Dordrecht., 61(5), 887-892.
https://doi.org/10.1007/s10722-014-0102-9

Mikić A, Medović A, Jovanović Ž, Stanisavljević N. Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum). in Genetic Resources and Crop Evolution. 2014;61(5):887-892.
doi:10.1007/s10722-014-0102-9 .

Mikić, Aleksandar, Medović, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, "Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)" in Genetic Resources and Crop Evolution, 61, no. 5 (2014):887-892,
https://doi.org/10.1007/s10722-014-0102-9 . .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Maksimović, Vesna R.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/742
AB  - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Plant Physiology and Biochemistry
T1  - Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)
EP  - 31
SP  - 26
VL  - 83
DO  - 10.1016/j.plaphy.2014.07.008
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, Aleksandar and Radović, Svetlana and Maksimović, Vesna R.",
year = "2014",
abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Plant Physiology and Biochemistry",
title = "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)",
pages = "31-26",
volume = "83",
doi = "10.1016/j.plaphy.2014.07.008"
}

Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2014). Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 83, 26-31.
https://doi.org/10.1016/j.plaphy.2014.07.008

Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry. 2014;83:26-31.
doi:10.1016/j.plaphy.2014.07.008 .

Jovanović, Živko, Stanisavljević, Nemanja, Mikić, Aleksandar, Radović, Svetlana, Maksimović, Vesna R., "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)" in Plant Physiology and Biochemistry, 83 (2014):26-31,
https://doi.org/10.1016/j.plaphy.2014.07.008 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Cupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljus Dukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/626
AB  - Antioxidant activities were studied in methanolic and water extracts of nonprocessed, cooked and in vitro enzymatically digested seed flour, as well as in total protein hydrolysates and small peptide fractions ( lt 3 and  lt 10kDa) of three pea and five grass pea cultivars. The antioxidative properties were determined by three spectrophotometric methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, Folin-Ciocalteu (FC) reducing capacity assay and H2O2 scavenging. We also applied one luminometric assay for hydroxyl radical scavenging. The study showed that cooking and enzymatic digestion strongly enhanced the release of phenolic compounds in methanolic extracts of four analysed cultivars. Scavenging activity against DPPH radical, hydroxyl radical and hydrogen peroxide was increased in majority of analysed flour specimens subjected to processing. Our findings indicate that, besides the phenolic compounds, the small peptide fraction, especially the MW  lt 3kDa, in methanolic and aqueous extracts of cooked and digested seed flour significantly contribute to free radical and hydrogen peroxide scavenging activity in all investigated cultivars. Our data strongly suggest that simple cooking treatment and in vitro digestion of seed flour applied prior to extraction with methanol could improve antioxidative activity of obtained extracts.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour
EP  - 2104
IS  - 10
SP  - 2096
VL  - 48
DO  - 10.1111/ijfs.12191
ER  - 

@article{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Cupić, Tihomir and Lukić, Jovanka and Miljus Dukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar",
year = "2013",
abstract = "Antioxidant activities were studied in methanolic and water extracts of nonprocessed, cooked and in vitro enzymatically digested seed flour, as well as in total protein hydrolysates and small peptide fractions ( lt 3 and  lt 10kDa) of three pea and five grass pea cultivars. The antioxidative properties were determined by three spectrophotometric methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, Folin-Ciocalteu (FC) reducing capacity assay and H2O2 scavenging. We also applied one luminometric assay for hydroxyl radical scavenging. The study showed that cooking and enzymatic digestion strongly enhanced the release of phenolic compounds in methanolic extracts of four analysed cultivars. Scavenging activity against DPPH radical, hydroxyl radical and hydrogen peroxide was increased in majority of analysed flour specimens subjected to processing. Our findings indicate that, besides the phenolic compounds, the small peptide fraction, especially the MW  lt 3kDa, in methanolic and aqueous extracts of cooked and digested seed flour significantly contribute to free radical and hydrogen peroxide scavenging activity in all investigated cultivars. Our data strongly suggest that simple cooking treatment and in vitro digestion of seed flour applied prior to extraction with methanol could improve antioxidative activity of obtained extracts.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour",
pages = "2104-2096",
number = "10",
volume = "48",
doi = "10.1111/ijfs.12191"
}

Stanisavljević, N., Jovanović, Ž., Cupić, T., Lukić, J., Miljus Dukić, J., Radović, S.,& Mikić, A.. (2013). Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour. in International Journal of Food Science and Technology
Wiley-Blackwell, Hoboken., 48(10), 2096-2104.
https://doi.org/10.1111/ijfs.12191

Stanisavljević N, Jovanović Ž, Cupić T, Lukić J, Miljus Dukić J, Radović S, Mikić A. Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour. in International Journal of Food Science and Technology. 2013;48(10):2096-2104.
doi:10.1111/ijfs.12191 .

Stanisavljević, Nemanja, Jovanović, Živko, Cupić, Tihomir, Lukić, Jovanka, Miljus Dukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, "Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour" in International Journal of Food Science and Technology, 48, no. 10 (2013):2096-2104,
https://doi.org/10.1111/ijfs.12191 . .

TY  - JOUR
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Ćupina, Branko
AU  - Jovanović, Živko
AU  - Radović, Svetlana
AU  - Nikolić, Aleksandra
AU  - Stanisavljević, Nemanja
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/481
AB  - Arheološki lokalitet Hisar je gradinsko naselje Brnjičke kulturne grupe (gvozdeno doba I u Pomoravlju), podignuto na vrhu, odnosno na platou brda Hisar koje kontroliše zonu ušća Jablanice i Veternice u Južnu Moravu i najveći deo Leskovačke kotline. Prilikom arheoloških istraživanja 2005. godine u objektima koji se vezuju za Brnjica II a fazu (12. vek pre nove ere) pronađene su dve velike količine ugljenisanih semena graška (Pisum sativum L) i urova (Vicia ervilia (L) Willd). Zalihe ove dve zrnaste mahunarke bile su skoro čiste, sa malim procentom nečistoća koje su pre svega činili plodovi, pre svega žitarica, drugih mahunarki (sočivo - Lens culinaris Medik. i bob - Vicia faba L), ali i biljaka uljarica, što ukazuje da su grašak i urov bili odvojeno uzgajani, a potom i odvojeno skladišteni. Ovi nalazi, a pre svega nesvakidašnji nalaz zalihe graška, potvrđuju dugu tradiciju uzgajanja mahunarki u leskovačkom kraju.
AB  - A lucky find of 2,572 charred pea (Pisum sativum L) seeds in a single archaeobotanical sample from the hill fort settlement Hissar near Leskovac represents a unique example in Bronze / Iron Age research in South East Europe. Another mass storage of bitter vetch (Vicia ervilia (L) Willd) from the same site and period (Brnjica cultural group, beginning of the Iron Age in the Morava valley) confirms a long tradition of the city of Leskovac region as a pulse crop production centre in modern Serbia. Both pulse storages were almost pure with small amount of admixture from other crops mainly cereals, other pulses, such as lentil (Lens culinaris Medik) and broad bean (Vicia faba L), and oil/fibre plants. Both pulses were cultivated as main crops and were stored separately.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part one: Two charred pulse crop storages of the fortified hill fort settlement Hissar in Leskovac, South Serbia
EP  - 226
IS  - 1
SP  - 219
VL  - 48
DO  - 10.5937/ratpov1101219M
ER  - 

@article{
author = "Medović, Aleksandar and Mikić, Aleksandar and Ćupina, Branko and Jovanović, Živko and Radović, Svetlana and Nikolić, Aleksandra and Stanisavljević, Nemanja",
year = "2011",
abstract = "Arheološki lokalitet Hisar je gradinsko naselje Brnjičke kulturne grupe (gvozdeno doba I u Pomoravlju), podignuto na vrhu, odnosno na platou brda Hisar koje kontroliše zonu ušća Jablanice i Veternice u Južnu Moravu i najveći deo Leskovačke kotline. Prilikom arheoloških istraživanja 2005. godine u objektima koji se vezuju za Brnjica II a fazu (12. vek pre nove ere) pronađene su dve velike količine ugljenisanih semena graška (Pisum sativum L) i urova (Vicia ervilia (L) Willd). Zalihe ove dve zrnaste mahunarke bile su skoro čiste, sa malim procentom nečistoća koje su pre svega činili plodovi, pre svega žitarica, drugih mahunarki (sočivo - Lens culinaris Medik. i bob - Vicia faba L), ali i biljaka uljarica, što ukazuje da su grašak i urov bili odvojeno uzgajani, a potom i odvojeno skladišteni. Ovi nalazi, a pre svega nesvakidašnji nalaz zalihe graška, potvrđuju dugu tradiciju uzgajanja mahunarki u leskovačkom kraju., A lucky find of 2,572 charred pea (Pisum sativum L) seeds in a single archaeobotanical sample from the hill fort settlement Hissar near Leskovac represents a unique example in Bronze / Iron Age research in South East Europe. Another mass storage of bitter vetch (Vicia ervilia (L) Willd) from the same site and period (Brnjica cultural group, beginning of the Iron Age in the Morava valley) confirms a long tradition of the city of Leskovac region as a pulse crop production centre in modern Serbia. Both pulse storages were almost pure with small amount of admixture from other crops mainly cereals, other pulses, such as lentil (Lens culinaris Medik) and broad bean (Vicia faba L), and oil/fibre plants. Both pulses were cultivated as main crops and were stored separately.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje, Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part one: Two charred pulse crop storages of the fortified hill fort settlement Hissar in Leskovac, South Serbia",
pages = "226-219",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101219M"
}

Medović, A., Mikić, A., Ćupina, B., Jovanović, Ž., Radović, S., Nikolić, A.,& Stanisavljević, N.. (2011). Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 219-226.
https://doi.org/10.5937/ratpov1101219M

Medović A, Mikić A, Ćupina B, Jovanović Ž, Radović S, Nikolić A, Stanisavljević N. Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje. in Ratarstvo i povrtarstvo. 2011;48(1):219-226.
doi:10.5937/ratpov1101219M .

Medović, Aleksandar, Mikić, Aleksandar, Ćupina, Branko, Jovanović, Živko, Radović, Svetlana, Nikolić, Aleksandra, Stanisavljević, Nemanja, "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje" in Ratarstvo i povrtarstvo, 48, no. 1 (2011):219-226,
https://doi.org/10.5937/ratpov1101219M . .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Aleksandra
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Đorđević, Vuk
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/504
AB  - Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.
AB  - The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia
EP  - 232
IS  - 1
SP  - 227
VL  - 48
DO  - 10.5937/ratpov1101227J
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Nikolić, Aleksandra and Medović, Aleksandar and Mikić, Aleksandar and Radović, Svetlana and Đorđević, Vuk",
year = "2011",
abstract = "Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou., The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji, Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia",
pages = "232-227",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101227J"
}

Jovanović, Ž., Stanisavljević, N., Nikolić, A., Medović, A., Mikić, A., Radović, S.,& Đorđević, V.. (2011). Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 227-232.
https://doi.org/10.5937/ratpov1101227J

Jovanović Ž, Stanisavljević N, Nikolić A, Medović A, Mikić A, Radović S, Đorđević V. Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji. in Ratarstvo i povrtarstvo. 2011;48(1):227-232.
doi:10.5937/ratpov1101227J .

Jovanović, Živko, Stanisavljević, Nemanja, Nikolić, Aleksandra, Medović, Aleksandar, Mikić, Aleksandar, Radović, Svetlana, Đorđević, Vuk, "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji" in Ratarstvo i povrtarstvo, 48, no. 1 (2011):227-232,
https://doi.org/10.5937/ratpov1101227J . .