TY  - JOUR
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Timotijević, Gordana
AU  - Miljković, Djordje
AU  - Donia, Marco
AU  - Libra, Massimo
AU  - Coco, Marinella
AU  - McCubrey, James
AU  - Al-Abed, Yousef
AU  - Korac, Aleksandra
AU  - Stošić-Grujičić, Stanislava
AU  - Nicoletti, Ferdinando
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/434
AB  - The new chemical entity GIT-27NO was created by the covalent linkage of a NO moiety to the antiinflammatory isoxazoline VGX-1027 The compound has been shown to possess powerful anticancer effects both in vitro and in vivo However, its effects on nonsolid and metastatic forms of tumors have not yet been investigated We have studied the effects of GIT-27NO on the highly invasive mouse mammary TA3Ha cell line in vitro and in vivo In contrast to the conventional exogenous NO donor sodium nitroprusside, GIT-27NO successfully enhanced intracellular NO concentration in TA3Ha cells Intracellular accumulation of NO was followed by marked decrease in TA3Ha cell viability accompanied by typical apoptotic features Interestingly, inverted membrane phosphatidylserine residues. reduced volume of nucleus, condensed chromatin, and terminal fragmentation of DNA were associated with inhibited caspase-3 activity and transcription of the genes encoding caspase-3, -8, and -9 In parallel, GIT-27NO rapidly but transiently prevented the loss of p53 through phosphorylation on Ser 20 and provided the necessary signals tor the execution of downstream processes without p53 de novo synthesis The caspase-independent apoptotic-like death process triggered by GIT-27NO could be mediated by markedly down-regulated expression of the antiapoptotic Bcl-2 molecule observed in TA3Ha cells exposed to GIT-27NO In agreement with these in vitro data, GIT-27NO efficiently suppressed the growth of the ascites form and associated-lethality of tumor induced by TA3Ha cells in mice
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology and Medicine
T1  - Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO
EP  - 1099
IS  - 8
SP  - 1090
VL  - 48
DO  - 10.1016/j.freeradbiomed.2010.01.026
ER  - 

@article{
author = "Mijatović, Sanja and Maksimović-Ivanić, Danijela and Timotijević, Gordana and Miljković, Djordje and Donia, Marco and Libra, Massimo and Coco, Marinella and McCubrey, James and Al-Abed, Yousef and Korac, Aleksandra and Stošić-Grujičić, Stanislava and Nicoletti, Ferdinando",
year = "2010",
abstract = "The new chemical entity GIT-27NO was created by the covalent linkage of a NO moiety to the antiinflammatory isoxazoline VGX-1027 The compound has been shown to possess powerful anticancer effects both in vitro and in vivo However, its effects on nonsolid and metastatic forms of tumors have not yet been investigated We have studied the effects of GIT-27NO on the highly invasive mouse mammary TA3Ha cell line in vitro and in vivo In contrast to the conventional exogenous NO donor sodium nitroprusside, GIT-27NO successfully enhanced intracellular NO concentration in TA3Ha cells Intracellular accumulation of NO was followed by marked decrease in TA3Ha cell viability accompanied by typical apoptotic features Interestingly, inverted membrane phosphatidylserine residues. reduced volume of nucleus, condensed chromatin, and terminal fragmentation of DNA were associated with inhibited caspase-3 activity and transcription of the genes encoding caspase-3, -8, and -9 In parallel, GIT-27NO rapidly but transiently prevented the loss of p53 through phosphorylation on Ser 20 and provided the necessary signals tor the execution of downstream processes without p53 de novo synthesis The caspase-independent apoptotic-like death process triggered by GIT-27NO could be mediated by markedly down-regulated expression of the antiapoptotic Bcl-2 molecule observed in TA3Ha cells exposed to GIT-27NO In agreement with these in vitro data, GIT-27NO efficiently suppressed the growth of the ascites form and associated-lethality of tumor induced by TA3Ha cells in mice",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO",
pages = "1099-1090",
number = "8",
volume = "48",
doi = "10.1016/j.freeradbiomed.2010.01.026"
}

Mijatović, S., Maksimović-Ivanić, D., Timotijević, G., Miljković, D., Donia, M., Libra, M., Coco, M., McCubrey, J., Al-Abed, Y., Korac, A., Stošić-Grujičić, S.,& Nicoletti, F.. (2010). Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 48(8), 1090-1099.
https://doi.org/10.1016/j.freeradbiomed.2010.01.026

Mijatović S, Maksimović-Ivanić D, Timotijević G, Miljković D, Donia M, Libra M, Coco M, McCubrey J, Al-Abed Y, Korac A, Stošić-Grujičić S, Nicoletti F. Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO. in Free Radical Biology and Medicine. 2010;48(8):1090-1099.
doi:10.1016/j.freeradbiomed.2010.01.026 .

Mijatović, Sanja, Maksimović-Ivanić, Danijela, Timotijević, Gordana, Miljković, Djordje, Donia, Marco, Libra, Massimo, Coco, Marinella, McCubrey, James, Al-Abed, Yousef, Korac, Aleksandra, Stošić-Grujičić, Stanislava, Nicoletti, Ferdinando, "Induction of caspase-independent apoptotic-like cell death of mouse mammary tumor TA3Ha cells in vitro and reduction of their lethality in vivo by the novel chemotherapeutic agent GIT-27NO" in Free Radical Biology and Medicine, 48, no. 8 (2010):1090-1099,
https://doi.org/10.1016/j.freeradbiomed.2010.01.026 . .