TY  - JOUR
AU  - Veljović, Katarina
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Mihajlović, Sanja
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Begović, Jelena
AU  - Mrvaljević, Igor
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1031
AB  - The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows
VL  - 8
DO  - 10.3389/fmicb.2017.02028
ER  - 

@article{
author = "Veljović, Katarina and Dinić, Miroslav and Lukić, Jovanka and Mihajlović, Sanja and Tolinački, Maja and Živković, Milica and Begović, Jelena and Mrvaljević, Igor and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows",
volume = "8",
doi = "10.3389/fmicb.2017.02028"
}

Veljović, K., Dinić, M., Lukić, J., Mihajlović, S., Tolinački, M., Živković, M., Begović, J., Mrvaljević, I., Golić, N.,& Terzić-Vidojević, A.. (2017). Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.02028

Veljović K, Dinić M, Lukić J, Mihajlović S, Tolinački M, Živković M, Begović J, Mrvaljević I, Golić N, Terzić-Vidojević A. Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.02028 .

Veljović, Katarina, Dinić, Miroslav, Lukić, Jovanka, Mihajlović, Sanja, Tolinački, Maja, Živković, Milica, Begović, Jelena, Mrvaljević, Igor, Golić, Nataša, Terzić-Vidojević, Amarela, "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.02028 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Strahinić, Ivana
AU  - Mrvaljević, Igor
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1091
AB  - Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.
PB  - BMC, London
T2  - BMC Microbiology
T1  - In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
VL  - 17
DO  - 10.1186/s12866-017-1015-5
ER  - 

@article{
author = "Golić, Nataša and Veljović, Katarina and Popović, Nikola and Đokić, Jelena and Strahinić, Ivana and Mrvaljević, Igor and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.",
publisher = "BMC, London",
journal = "BMC Microbiology",
title = "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens",
volume = "17",
doi = "10.1186/s12866-017-1015-5"
}

Golić, N., Veljović, K., Popović, N., Đokić, J., Strahinić, I., Mrvaljević, I.,& Terzić-Vidojević, A.. (2017). In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology
BMC, London., 17.
https://doi.org/10.1186/s12866-017-1015-5

Golić N, Veljović K, Popović N, Đokić J, Strahinić I, Mrvaljević I, Terzić-Vidojević A. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology. 2017;17.
doi:10.1186/s12866-017-1015-5 .

Golić, Nataša, Veljović, Katarina, Popović, Nikola, Đokić, Jelena, Strahinić, Ivana, Mrvaljević, Igor, Terzić-Vidojević, Amarela, "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens" in BMC Microbiology, 17 (2017),
https://doi.org/10.1186/s12866-017-1015-5 . .