TY  - CONF
AU  - Golić, Nataša
AU  - Terzić Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Dinić, Miroslav
AU  - Đokić, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Lukić, Jovanka
AU  - Živković, Milica
AU  - Nastasijević, Branislav
AU  - Soković, Svetlana
AU  - Brdarić, Emilija
AU  - Radojević, Dušan
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2372
AB  - There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS
EP  - 106
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2372
ER  - 

@conference{
author = "Golić, Nataša and Terzić Vidojević, Amarela and Tolinački, Maja and Dinić, Miroslav and Đokić, Jelena and Todorović Vukotić, Nevena and Lukić, Jovanka and Živković, Milica and Nastasijević, Branislav and Soković, Svetlana and Brdarić, Emilija and Radojević, Dušan",
year = "2024",
abstract = "There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS",
pages = "106-106",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2372"
}

Golić, N., Terzić Vidojević, A., Tolinački, M., Dinić, M., Đokić, J., Todorović Vukotić, N., Lukić, J., Živković, M., Nastasijević, B., Soković, S., Brdarić, E.,& Radojević, D.. (2024). THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372

Golić N, Terzić Vidojević A, Tolinački M, Dinić M, Đokić J, Todorović Vukotić N, Lukić J, Živković M, Nastasijević B, Soković S, Brdarić E, Radojević D. THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

Golić, Nataša, Terzić Vidojević, Amarela, Tolinački, Maja, Dinić, Miroslav, Đokić, Jelena, Todorović Vukotić, Nevena, Lukić, Jovanka, Živković, Milica, Nastasijević, Branislav, Soković, Svetlana, Brdarić, Emilija, Radojević, Dušan, "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):106-106,
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

TY  - JOUR
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Brdarić, Emilija
AU  - Bajić, Svetlana Soković
AU  - Đokić, Jelena
AU  - Živković, Milica
AU  - Veljović, Katarina
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/47047
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2296
AB  - One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains
T1  - Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana
EP  - 570
IS  - Notebook 6
SP  - 554
VL  - 73
DO  - 10.5937/arhfarm73-47047
ER  - 

@article{
author = "Popović, Nikola and Terzić-Vidojević, Amarela and Brdarić, Emilija and Bajić, Svetlana Soković and Đokić, Jelena and Živković, Milica and Veljović, Katarina",
year = "2023",
abstract = "One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains, Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana",
pages = "570-554",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-47047"
}

Popović, N., Terzić-Vidojević, A., Brdarić, E., Bajić, S. S., Đokić, J., Živković, M.,& Veljović, K.. (2023). Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 554-570.
https://doi.org/10.5937/arhfarm73-47047

Popović N, Terzić-Vidojević A, Brdarić E, Bajić SS, Đokić J, Živković M, Veljović K. Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy. 2023;73(Notebook 6):554-570.
doi:10.5937/arhfarm73-47047 .

Popović, Nikola, Terzić-Vidojević, Amarela, Brdarić, Emilija, Bajić, Svetlana Soković, Đokić, Jelena, Živković, Milica, Veljović, Katarina, "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains" in Archives of Pharmacy, 73, no. Notebook 6 (2023):554-570,
https://doi.org/10.5937/arhfarm73-47047 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Đokić, Jelena
AU  - Tolinački, Maja
AU  - Živković, Milica
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46921
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2295
AB  - In recent years, a vast number of human diseases have been correlated with gut microbiota dysbiosis. The development of modern methods in molecular microbiology, such as the culturomics approach, as well as various multi-omics methods like next generation sequencing, transcriptomics and metabolomics analysis, coupled with large data sets correlation analysis, enabled the cultivation and characterization of novel anaerobic hitherto uncultivated Next-Generation Probiotics. In addition, the results of host-microbe interactions studies helped to reveal the mechanisms involved in the beneficial effects of Next-Generation Probiotics. Eventually, the obtained data on Next-Generation Probiotics will help to broaden the scientific knowledge on these bacteria, in terms of both their safety and health-promoting effects, unravel opportunities for the development of novel therapeutic strategies for prevention and treatment of tumors, metabolic, neuropsychiatric and other diseases, with the aim of relieving the symptoms of the diseases and increasing the quality of life for patients and their families. So far, the best characterized probiotics of the new generation are Akkermansia muciniphila, Faecalibacterium prauznitzii and Bacteroides fragilis.
AB  - Poslednjih  godina  se  veliki  broj  patoloških  stanja  i  bolesti  dovodi  u  vezu sa  disbiozom  crevne   mikrobiote   i   promenama   u   njenom   funkcionisanju.   Razvoj   savremenih   metoda   molekularne  mikrobiologije,  uključujući   kulturomiku   i   integrativne   pristupe   kao   što   su   sekvenciranje  sledeće  generacije,  transkriptomska  analiza  dualne  RNK  sekvence  i  analiza  metabolomike, omogućio je identifikaciju, kultivaciju i karakterizaciju novih anaerobnih, do sada nekultivisanih probiotika, nazvanih probiotici sledeće generacije. Pored toga, rezultati in vitro i in  vivostudija proučavanja interakcija domaćina  sa  mikrobiotom  pomogli  su  u  rasvetljavanju  mehanizama  delovanja  probiotika  sledeće  generacije.  Na  kraju,  dobijeni  podaci  o  probioticima  sledeće generacije pomoći će da se prošire naučna saznanja o ovim bakterijama, kako u pogledu njihove bezbednosti, tako i u pogledu njihovog uticaja na zdravlje, otvarajući mogućnost za nove terapijske pristupe u prevenciji i terapiji metaboličkih bolesti, tumora, neurodegenerativnih i psihijatrijskih bolesti i drugih bolesti, u cilju ublažavanja simptoma bolesti i poboljšanja kvaliteta života pacijenata i njihovih porodica. Do sada najbolje opisani probiotici sledeće generacije su Akkermansia muciniphila, Fecalibacterium prauznitzii i Bacteroides fragilis
PB  - Beograd : Savez farmaceutskih udruženja Srbije
PB  - Beograd : Univerzitet u Beogradu - Farmaceutski fakultet
T2  - Archives of Pharmacy
T1  - Next-Generation Probiotics: health-promoting bacteria of the human gut
EP  - 534
IS  - Notebook 6
SP  - 515
VL  - 73
DO  - 10.5937/arhfarm73-46921
ER  - 

@article{
author = "Golić, Nataša and Đokić, Jelena and Tolinački, Maja and Živković, Milica",
year = "2023",
abstract = "In recent years, a vast number of human diseases have been correlated with gut microbiota dysbiosis. The development of modern methods in molecular microbiology, such as the culturomics approach, as well as various multi-omics methods like next generation sequencing, transcriptomics and metabolomics analysis, coupled with large data sets correlation analysis, enabled the cultivation and characterization of novel anaerobic hitherto uncultivated Next-Generation Probiotics. In addition, the results of host-microbe interactions studies helped to reveal the mechanisms involved in the beneficial effects of Next-Generation Probiotics. Eventually, the obtained data on Next-Generation Probiotics will help to broaden the scientific knowledge on these bacteria, in terms of both their safety and health-promoting effects, unravel opportunities for the development of novel therapeutic strategies for prevention and treatment of tumors, metabolic, neuropsychiatric and other diseases, with the aim of relieving the symptoms of the diseases and increasing the quality of life for patients and their families. So far, the best characterized probiotics of the new generation are Akkermansia muciniphila, Faecalibacterium prauznitzii and Bacteroides fragilis., Poslednjih  godina  se  veliki  broj  patoloških  stanja  i  bolesti  dovodi  u  vezu sa  disbiozom  crevne   mikrobiote   i   promenama   u   njenom   funkcionisanju.   Razvoj   savremenih   metoda   molekularne  mikrobiologije,  uključujući   kulturomiku   i   integrativne   pristupe   kao   što   su   sekvenciranje  sledeće  generacije,  transkriptomska  analiza  dualne  RNK  sekvence  i  analiza  metabolomike, omogućio je identifikaciju, kultivaciju i karakterizaciju novih anaerobnih, do sada nekultivisanih probiotika, nazvanih probiotici sledeće generacije. Pored toga, rezultati in vitro i in  vivostudija proučavanja interakcija domaćina  sa  mikrobiotom  pomogli  su  u  rasvetljavanju  mehanizama  delovanja  probiotika  sledeće  generacije.  Na  kraju,  dobijeni  podaci  o  probioticima  sledeće generacije pomoći će da se prošire naučna saznanja o ovim bakterijama, kako u pogledu njihove bezbednosti, tako i u pogledu njihovog uticaja na zdravlje, otvarajući mogućnost za nove terapijske pristupe u prevenciji i terapiji metaboličkih bolesti, tumora, neurodegenerativnih i psihijatrijskih bolesti i drugih bolesti, u cilju ublažavanja simptoma bolesti i poboljšanja kvaliteta života pacijenata i njihovih porodica. Do sada najbolje opisani probiotici sledeće generacije su Akkermansia muciniphila, Fecalibacterium prauznitzii i Bacteroides fragilis",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije, Beograd : Univerzitet u Beogradu - Farmaceutski fakultet",
journal = "Archives of Pharmacy",
title = "Next-Generation Probiotics: health-promoting bacteria of the human gut",
pages = "534-515",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-46921"
}

Golić, N., Đokić, J., Tolinački, M.,& Živković, M.. (2023). Next-Generation Probiotics: health-promoting bacteria of the human gut. in Archives of Pharmacy
Beograd : Savez farmaceutskih udruženja Srbije., 73(Notebook 6), 515-534.
https://doi.org/10.5937/arhfarm73-46921

Golić N, Đokić J, Tolinački M, Živković M. Next-Generation Probiotics: health-promoting bacteria of the human gut. in Archives of Pharmacy. 2023;73(Notebook 6):515-534.
doi:10.5937/arhfarm73-46921 .

Golić, Nataša, Đokić, Jelena, Tolinački, Maja, Živković, Milica, "Next-Generation Probiotics: health-promoting bacteria of the human gut" in Archives of Pharmacy, 73, no. Notebook 6 (2023):515-534,
https://doi.org/10.5937/arhfarm73-46921 . .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - https://www.microbiota-site.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2186
AB  - Introduction: Exopolysaccharides (EPS) are carbohydrate polymers with important biological activities such 
as immunomodulatory, antioxidative and antitumor (1). How EPS affect the gut microbiota, which plays an 
important role in maintaining homeostasis in the organism, is poorly understood to date. Therefore, the aim 
of this study was to investigate how EPS-AN8, derived from Lactiplantibacillus plantarum BGAN8, affects part 
of the gut microbiota in the duodenum of healthy Dark Agouti rats (DA).
Materials and Methods: EPS-AN8 was administered to male DA at low (0.1 mg/mL) and high (1 mg/mL) 
dose over a 15-day period. Total duodenal DNA was isolated and PCR amplicon for 16SrRNA was sequenced 
on Illumina NovaSeq paired-end platform. Furthermore, we tracked key parameters of oxidative stress and 
inflammation in duodenal homogenates.
Results: The higher dose of EPS-AN8 resulted in an increased Shannon's diversity index. The most 
significant differences were observed in the increased relative abundance of the genera Ruminococcus, 
Dubosiella, Enterohabdus, and Adlercreutzia. At the same time, we demonstrated that neither dosage caused 
negative side effects such as inflammation and oxidative stress.
Conclusion: Considering the existing trend to market foods with additional health benefits, our results 
suggest that EPS-AN8 could be a good candidate for functional food supplementation.
C3  - 10th ISM World Congress on  Targeting Microbiota
T1  - CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM  BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT
EP  - 61
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2186
ER  - 

@conference{
author = "Brdarić, Emilija and Soković Bajić, Svetlana and Popović, Dušanka and Kulaš, Jelena and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Introduction: Exopolysaccharides (EPS) are carbohydrate polymers with important biological activities such 
as immunomodulatory, antioxidative and antitumor (1). How EPS affect the gut microbiota, which plays an 
important role in maintaining homeostasis in the organism, is poorly understood to date. Therefore, the aim 
of this study was to investigate how EPS-AN8, derived from Lactiplantibacillus plantarum BGAN8, affects part 
of the gut microbiota in the duodenum of healthy Dark Agouti rats (DA).
Materials and Methods: EPS-AN8 was administered to male DA at low (0.1 mg/mL) and high (1 mg/mL) 
dose over a 15-day period. Total duodenal DNA was isolated and PCR amplicon for 16SrRNA was sequenced 
on Illumina NovaSeq paired-end platform. Furthermore, we tracked key parameters of oxidative stress and 
inflammation in duodenal homogenates.
Results: The higher dose of EPS-AN8 resulted in an increased Shannon's diversity index. The most 
significant differences were observed in the increased relative abundance of the genera Ruminococcus, 
Dubosiella, Enterohabdus, and Adlercreutzia. At the same time, we demonstrated that neither dosage caused 
negative side effects such as inflammation and oxidative stress.
Conclusion: Considering the existing trend to market foods with additional health benefits, our results 
suggest that EPS-AN8 could be a good candidate for functional food supplementation.",
journal = "10th ISM World Congress on  Targeting Microbiota",
title = "CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM  BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2186"
}

Brdarić, E., Soković Bajić, S., Popović, D., Kulaš, J., Popov Aleksandrov, A., Mirkov, I.,& Živković, M.. (2023). CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM  BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT. in 10th ISM World Congress on  Targeting Microbiota, 61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2186

Brdarić E, Soković Bajić S, Popović D, Kulaš J, Popov Aleksandrov A, Mirkov I, Živković M. CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM  BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT. in 10th ISM World Congress on  Targeting Microbiota. 2023;:61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2186 .

Brdarić, Emilija, Soković Bajić, Svetlana, Popović, Dušanka, Kulaš, Jelena, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Živković, Milica, "CONSUMPTION OF EXOPOLYSACCHARIDES FROM LACTIPLANTIBACILLUS PLANTARUM  BGAN8 ALTERS THE GUT MICROBIOTA OF DA RAT" in 10th ISM World Congress on  Targeting Microbiota (2023):61-61,
https://hdl.handle.net/21.15107/rcub_imagine_2186 .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2164
AB  - Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом.
AB  - Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
T1  - Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)
T1  - Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2164
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом., Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia",
title = "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II), Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2164"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_imagine_2164

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2164 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)" in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2164 .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Aleksandrov, Aleksandra Popov
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1713
AB  - Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS) derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation (IL-1β, TNF-α, and IFN-γ) in the duodenum. Moreover, micrographs of the duodenum were in line with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used 16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting this toxic environmental pollutant.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats
IS  - 3
SP  - 2845
VL  - 24
DO  - 10.3390/ijms24032845
ER  - 

@article{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Aleksandrov, Aleksandra Popov and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS) derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation (IL-1β, TNF-α, and IFN-γ) in the duodenum. Moreover, micrographs of the duodenum were in line with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used 16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting this toxic environmental pollutant.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats",
number = "3",
pages = "2845",
volume = "24",
doi = "10.3390/ijms24032845"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Aleksandrov, A. P., Golić, N., Mirkov, I.,& Živković, M.. (2023). Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences, 24(3), 2845.
https://doi.org/10.3390/ijms24032845

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Aleksandrov AP, Golić N, Mirkov I, Živković M. Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences. 2023;24(3):2845.
doi:10.3390/ijms24032845 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Aleksandrov, Aleksandra Popov, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats" in International Journal of Molecular Sciences, 24, no. 3 (2023):2845,
https://doi.org/10.3390/ijms24032845 . .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Durdić, Sladana
AU  - Ruas-Madiedo, Patricia
AU  - Stevanović, Magdalena
AU  - Tolinački, Maja
AU  - Dinić, Miroslav
AU  - Mutić, Jelena
AU  - Golić, Nataša
AU  - Živković, Milica
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1471
AB  - Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells
VL  - 12
DO  - 10.3389/fmicb.2021.759378
ER  - 

@article{
author = "Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Durdić, Sladana and Ruas-Madiedo, Patricia and Stevanović, Magdalena and Tolinački, Maja and Dinić, Miroslav and Mutić, Jelena and Golić, Nataša and Živković, Milica",
year = "2021",
abstract = "Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells",
volume = "12",
doi = "10.3389/fmicb.2021.759378"
}

Brdarić, E., Soković Bajić, S., Đokić, J., Durdić, S., Ruas-Madiedo, P., Stevanović, M., Tolinački, M., Dinić, M., Mutić, J., Golić, N.,& Živković, M.. (2021). Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fmicb.2021.759378

Brdarić E, Soković Bajić S, Đokić J, Durdić S, Ruas-Madiedo P, Stevanović M, Tolinački M, Dinić M, Mutić J, Golić N, Živković M. Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology. 2021;12.
doi:10.3389/fmicb.2021.759378 .

Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Durdić, Sladana, Ruas-Madiedo, Patricia, Stevanović, Magdalena, Tolinački, Maja, Dinić, Miroslav, Mutić, Jelena, Golić, Nataša, Živković, Milica, "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells" in Frontiers in Microbiology, 12 (2021),
https://doi.org/10.3389/fmicb.2021.759378 . .

TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1395
AB  - Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - Journal of Clinical Gastroenterology
T1  - The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11
EP  - S12
SP  - S11
VL  - 54
DO  - 10.1097/MCG.0000000000001292
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1395
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2020",
abstract = "Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "Journal of Clinical Gastroenterology",
title = "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11",
pages = "S12-S11",
volume = "54",
doi = "10.1097/MCG.0000000000001292",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1395"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2020). The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology
Wolters Kluwer Health., 54, S11-S12.
https://doi.org/10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology. 2020;54:S11-S12.
doi:10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11" in Journal of Clinical Gastroenterology, 54 (2020):S11-S12,
https://doi.org/10.1097/MCG.0000000000001292 .,
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Vidić, Jasmina
AU  - Vizzini, Priya
AU  - Manzano, Marisa
AU  - Kavanaugh, Devon
AU  - Ramarao, Nalini
AU  - Živković, Milica
AU  - Radonić, Vasa
AU  - Knezević, Nikola
AU  - Giouroudi, Ioanna
AU  - Gadjanski, Ivana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1296
AB  - Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
PB  - MDPI, Basel
T2  - Sensors
T1  - Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
IS  - 5
VL  - 19
DO  - 10.3390/s19051100
ER  - 

@article{
author = "Vidić, Jasmina and Vizzini, Priya and Manzano, Marisa and Kavanaugh, Devon and Ramarao, Nalini and Živković, Milica and Radonić, Vasa and Knezević, Nikola and Giouroudi, Ioanna and Gadjanski, Ivana",
year = "2019",
abstract = "Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.",
publisher = "MDPI, Basel",
journal = "Sensors",
title = "Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria",
number = "5",
volume = "19",
doi = "10.3390/s19051100"
}

Vidić, J., Vizzini, P., Manzano, M., Kavanaugh, D., Ramarao, N., Živković, M., Radonić, V., Knezević, N., Giouroudi, I.,& Gadjanski, I.. (2019). Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria. in Sensors
MDPI, Basel., 19(5).
https://doi.org/10.3390/s19051100

Vidić J, Vizzini P, Manzano M, Kavanaugh D, Ramarao N, Živković M, Radonić V, Knezević N, Giouroudi I, Gadjanski I. Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria. in Sensors. 2019;19(5).
doi:10.3390/s19051100 .

Vidić, Jasmina, Vizzini, Priya, Manzano, Marisa, Kavanaugh, Devon, Ramarao, Nalini, Živković, Milica, Radonić, Vasa, Knezević, Nikola, Giouroudi, Ioanna, Gadjanski, Ivana, "Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria" in Sensors, 19, no. 5 (2019),
https://doi.org/10.3390/s19051100 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Mihajlović, Sanja
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Begović, Jelena
AU  - Mrvaljević, Igor
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1031
AB  - The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows
VL  - 8
DO  - 10.3389/fmicb.2017.02028
ER  - 

@article{
author = "Veljović, Katarina and Dinić, Miroslav and Lukić, Jovanka and Mihajlović, Sanja and Tolinački, Maja and Živković, Milica and Begović, Jelena and Mrvaljević, Igor and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows",
volume = "8",
doi = "10.3389/fmicb.2017.02028"
}

Veljović, K., Dinić, M., Lukić, J., Mihajlović, S., Tolinački, M., Živković, M., Begović, J., Mrvaljević, I., Golić, N.,& Terzić-Vidojević, A.. (2017). Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.02028

Veljović K, Dinić M, Lukić J, Mihajlović S, Tolinački M, Živković M, Begović J, Mrvaljević I, Golić N, Terzić-Vidojević A. Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.02028 .

Veljović, Katarina, Dinić, Miroslav, Lukić, Jovanka, Mihajlović, Sanja, Tolinački, Maja, Živković, Milica, Begović, Jelena, Mrvaljević, Igor, Golić, Nataša, Terzić-Vidojević, Amarela, "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.02028 . .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Živković, Milica
AU  - Arambasić-Jovanović, Jelena
AU  - Tolinački, Maja
AU  - Sinadinović, Marija
AU  - Rajić, Jovana
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Golić, Nataša
AU  - Vidaković, Melita
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1039
AB  - The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBP beta. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in alpha-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats
EP  - 437
SP  - 427
VL  - 38
DO  - 10.1016/j.jff.2017.09.033
ER  - 

@article{
author = "Mihailović, Mirjana and Živković, Milica and Arambasić-Jovanović, Jelena and Tolinački, Maja and Sinadinović, Marija and Rajić, Jovana and Uskoković, Aleksandra and Dinić, Svetlana and Grdović, Nevena and Golić, Nataša and Vidaković, Melita",
year = "2017",
abstract = "The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBP beta. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in alpha-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats",
pages = "437-427",
volume = "38",
doi = "10.1016/j.jff.2017.09.033"
}

Mihailović, M., Živković, M., Arambasić-Jovanović, J., Tolinački, M., Sinadinović, M., Rajić, J., Uskoković, A., Dinić, S., Grdović, N., Golić, N.,& Vidaković, M.. (2017). Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 38, 427-437.
https://doi.org/10.1016/j.jff.2017.09.033

Mihailović M, Živković M, Arambasić-Jovanović J, Tolinački M, Sinadinović M, Rajić J, Uskoković A, Dinić S, Grdović N, Golić N, Vidaković M. Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods. 2017;38:427-437.
doi:10.1016/j.jff.2017.09.033 .

Mihailović, Mirjana, Živković, Milica, Arambasić-Jovanović, Jelena, Tolinački, Maja, Sinadinović, Marija, Rajić, Jovana, Uskoković, Aleksandra, Dinić, Svetlana, Grdović, Nevena, Golić, Nataša, Vidaković, Melita, "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats" in Journal of Functional Foods, 38 (2017):427-437,
https://doi.org/10.1016/j.jff.2017.09.033 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Miljković, Marge S.
AU  - Ruas-Madiedo, Patricia
AU  - Markelić, Milica B.
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Soković Bajić, Svetlana
AU  - Korac, Aleksandra
AU  - Golić, Nataša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/989
AB  - The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells
VL  - 7
DO  - 10.3389/fmicb.2016.00286
ER  - 

@article{
author = "Živković, Milica and Miljković, Marge S. and Ruas-Madiedo, Patricia and Markelić, Milica B. and Veljović, Katarina and Tolinački, Maja and Soković Bajić, Svetlana and Korac, Aleksandra and Golić, Nataša",
year = "2016",
abstract = "The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells",
volume = "7",
doi = "10.3389/fmicb.2016.00286"
}

Živković, M., Miljković, M. S., Ruas-Madiedo, P., Markelić, M. B., Veljović, K., Tolinački, M., Soković Bajić, S., Korac, A.,& Golić, N.. (2016). EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.00286

Živković M, Miljković MS, Ruas-Madiedo P, Markelić MB, Veljović K, Tolinački M, Soković Bajić S, Korac A, Golić N. EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.00286 .

Živković, Milica, Miljković, Marge S., Ruas-Madiedo, Patricia, Markelić, Milica B., Veljović, Katarina, Tolinački, Maja, Soković Bajić, Svetlana, Korac, Aleksandra, Golić, Nataša, "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.00286 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Miljković, Marija
AU  - Ruas-Madiedo, Patricia
AU  - Strahinić, Ivana
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/886
AB  - Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from a soft, white, artisanal cheese, produces a high-molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of the strain. In this study, a 26.4-kb region originating from the pCG1 plasmid, previously shown to be responsible for the production of EPS-CG11 and a ropy phenotype, was cloned, sequenced, and functionally characterized. In this region 16 putative open reading frames (ORFs), encoding enzymes for the production of EPS-CG11, were organized in specific loci involved in the biosynthesis of the repeat unit, polymerization, export, regulation, and chain length determination. Interestingly, downstream of the eps gene cluster, a putative transposase gene was identified, followed by an additional rfb gene cluster containing the rfbACBD genes, the ones most probably responsible for dTDP-L-rhamnose biosynthesis. The functional analysis showed that the production of the high-molecular-weight fraction of EPS-CG11 was absent in two knockout mutants, one in the eps and the other in the rfb gene cluster, as confirmed by size exclusion chromatography analysis. Therefore, both eps and rfb genes clusters are prerequisites for the production of high-molecular-weight EPS-CG11 and for the ropy phenotype of strain L. paraplantarum BGCG11.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11
EP  - 1396
IS  - 4
SP  - 1387
VL  - 81
DO  - 10.1128/AEM.03028-14
ER  - 

@article{
author = "Živković, Milica and Miljković, Marija and Ruas-Madiedo, Patricia and Strahinić, Ivana and Tolinački, Maja and Golić, Nataša and Kojić, Milan",
year = "2015",
abstract = "Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from a soft, white, artisanal cheese, produces a high-molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of the strain. In this study, a 26.4-kb region originating from the pCG1 plasmid, previously shown to be responsible for the production of EPS-CG11 and a ropy phenotype, was cloned, sequenced, and functionally characterized. In this region 16 putative open reading frames (ORFs), encoding enzymes for the production of EPS-CG11, were organized in specific loci involved in the biosynthesis of the repeat unit, polymerization, export, regulation, and chain length determination. Interestingly, downstream of the eps gene cluster, a putative transposase gene was identified, followed by an additional rfb gene cluster containing the rfbACBD genes, the ones most probably responsible for dTDP-L-rhamnose biosynthesis. The functional analysis showed that the production of the high-molecular-weight fraction of EPS-CG11 was absent in two knockout mutants, one in the eps and the other in the rfb gene cluster, as confirmed by size exclusion chromatography analysis. Therefore, both eps and rfb genes clusters are prerequisites for the production of high-molecular-weight EPS-CG11 and for the ropy phenotype of strain L. paraplantarum BGCG11.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11",
pages = "1396-1387",
number = "4",
volume = "81",
doi = "10.1128/AEM.03028-14"
}

Živković, M., Miljković, M., Ruas-Madiedo, P., Strahinić, I., Tolinački, M., Golić, N.,& Kojić, M.. (2015). Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 81(4), 1387-1396.
https://doi.org/10.1128/AEM.03028-14

Živković M, Miljković M, Ruas-Madiedo P, Strahinić I, Tolinački M, Golić N, Kojić M. Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11. in Applied and Environmental Microbiology. 2015;81(4):1387-1396.
doi:10.1128/AEM.03028-14 .

Živković, Milica, Miljković, Marija, Ruas-Madiedo, Patricia, Strahinić, Ivana, Tolinački, Maja, Golić, Nataša, Kojić, Milan, "Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11" in Applied and Environmental Microbiology, 81, no. 4 (2015):1387-1396,
https://doi.org/10.1128/AEM.03028-14 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Hidalgo-Cantabrana, Claudio
AU  - Kojić, Milan
AU  - Gueimonde, Miguel
AU  - Golić, Nataša
AU  - Ruas-Madiedo, Patricia
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1623
AB  - The putative protective role of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11, and its non-EPS-producing isogenic strain NB1, was tested upon HT29-MTX monolayers challenged with seven opportunistic pathogens. The probiotic strain Lactobacillus rhamnosus LMG18243 (GG) was used as a reference bacterium. Tested lactobacilli were able to efficiently reduce the attachment to HT29-MTX of most pathogens. Lb. paraplantarum NB1 and Lb. rhamnosus GG were more efficient reducing the adhesion of Clostridium difficile or Yersinia enterocolitica than Lb. paraplantarum BGCG11, while stain BGCG11 reduced, to a greater extent, the adhesion of Escherichia coli and Listeria monocytogenes. The detachment and cell lysis of HT29-MTX monolayers in the presence of pathogens alone and co-incubated with lactobacilli or purified EPS was followed. L. monocytogenes induced the strongest cell detachment among the seven tested pathogens and this effect was prevented by addition of purified EPS-CG11. The results suggest that this EPS could be an effective macromolecule in protection of HT29-MTX cells from the pathogen-induced lysis. Regarding innate intestinal barrier, the presence of C. difficile induced the highest IL-8 production in HT29-MTX cells and this capability was reinforced by the co-incubation with Lb. paraplantarum NB1 and Lb. rhamnosus GG. However, the increase in IL-8 production was not noticed when C difficile was co-incubated with EPS-producing Lb. paraplantarum BGCG11 strain or its purified EPS-CG11 polymer, thus indicating that the polymer could hinder the contact of bacteria with the intestinal epithelium. The measurement of mucus secreted by HT29-MTX and the expression of mud, muc2, muc3B and muc5AC genes in the presence of pathogens and lactobacilli suggested that all lactobacilli strains are weak "co-adjuvants" helping some pathogens to slightly increase the secretion of mucus by HT29-MTX, while purified EPS-CG11 did not induce mucus secretion. Taking altogether, Lb. paraplantarum BGCG11 could act towards the reinforcement of the innate mucosal barrier through the synthesis of a physical-protective EPS layer which could make difficult the contact of the pathogens with the epithelial cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX
EP  - 207
SP  - 199
VL  - 74
DO  - 10.1016/j.foodres.2015.05.012
ER  - 

@article{
author = "Živković, Milica and Hidalgo-Cantabrana, Claudio and Kojić, Milan and Gueimonde, Miguel and Golić, Nataša and Ruas-Madiedo, Patricia",
year = "2015",
abstract = "The putative protective role of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11, and its non-EPS-producing isogenic strain NB1, was tested upon HT29-MTX monolayers challenged with seven opportunistic pathogens. The probiotic strain Lactobacillus rhamnosus LMG18243 (GG) was used as a reference bacterium. Tested lactobacilli were able to efficiently reduce the attachment to HT29-MTX of most pathogens. Lb. paraplantarum NB1 and Lb. rhamnosus GG were more efficient reducing the adhesion of Clostridium difficile or Yersinia enterocolitica than Lb. paraplantarum BGCG11, while stain BGCG11 reduced, to a greater extent, the adhesion of Escherichia coli and Listeria monocytogenes. The detachment and cell lysis of HT29-MTX monolayers in the presence of pathogens alone and co-incubated with lactobacilli or purified EPS was followed. L. monocytogenes induced the strongest cell detachment among the seven tested pathogens and this effect was prevented by addition of purified EPS-CG11. The results suggest that this EPS could be an effective macromolecule in protection of HT29-MTX cells from the pathogen-induced lysis. Regarding innate intestinal barrier, the presence of C. difficile induced the highest IL-8 production in HT29-MTX cells and this capability was reinforced by the co-incubation with Lb. paraplantarum NB1 and Lb. rhamnosus GG. However, the increase in IL-8 production was not noticed when C difficile was co-incubated with EPS-producing Lb. paraplantarum BGCG11 strain or its purified EPS-CG11 polymer, thus indicating that the polymer could hinder the contact of bacteria with the intestinal epithelium. The measurement of mucus secreted by HT29-MTX and the expression of mud, muc2, muc3B and muc5AC genes in the presence of pathogens and lactobacilli suggested that all lactobacilli strains are weak "co-adjuvants" helping some pathogens to slightly increase the secretion of mucus by HT29-MTX, while purified EPS-CG11 did not induce mucus secretion. Taking altogether, Lb. paraplantarum BGCG11 could act towards the reinforcement of the innate mucosal barrier through the synthesis of a physical-protective EPS layer which could make difficult the contact of the pathogens with the epithelial cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX",
pages = "207-199",
volume = "74",
doi = "10.1016/j.foodres.2015.05.012"
}

Živković, M., Hidalgo-Cantabrana, C., Kojić, M., Gueimonde, M., Golić, N.,& Ruas-Madiedo, P.. (2015). Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. in Food Research International
Elsevier Science Bv, Amsterdam., 74, 199-207.
https://doi.org/10.1016/j.foodres.2015.05.012

Živković M, Hidalgo-Cantabrana C, Kojić M, Gueimonde M, Golić N, Ruas-Madiedo P. Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. in Food Research International. 2015;74:199-207.
doi:10.1016/j.foodres.2015.05.012 .

Živković, Milica, Hidalgo-Cantabrana, Claudio, Kojić, Milan, Gueimonde, Miguel, Golić, Nataša, Ruas-Madiedo, Patricia, "Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX" in Food Research International, 74 (2015):199-207,
https://doi.org/10.1016/j.foodres.2015.05.012 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Hidalgo-Cantabrana, Claudio
AU  - Kojić, Milan
AU  - Gueimonde, Miguel
AU  - Golić, Nataša
AU  - Ruas-Madiedo, Patricia
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/794
AB  - The putative protective role of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11, and its non-EPS-producing isogenic strain NB1, was tested upon HT29-MTX monolayers challenged with seven opportunistic pathogens. The probiotic strain Lactobacillus rhamnosus LMG18243 (GG) was used as a reference bacterium. Tested lactobacilli were able to efficiently reduce the attachment to HT29-MTX of most pathogens. Lb. paraplantarum NB1 and Lb. rhamnosus GG were more efficient reducing the adhesion of Clostridium difficile or Yersinia enterocolitica than Lb. paraplantarum BGCG11, while stain BGCG11 reduced, to a greater extent, the adhesion of Escherichia coli and Listeria monocytogenes. The detachment and cell lysis of HT29-MTX monolayers in the presence of pathogens alone and co-incubated with lactobacilli or purified EPS was followed. L. monocytogenes induced the strongest cell detachment among the seven tested pathogens and this effect was prevented by addition of purified EPS-CG11. The results suggest that this EPS could be an effective macromolecule in protection of HT29-MTX cells from the pathogen-induced lysis. Regarding innate intestinal barrier, the presence of C. difficile induced the highest IL-8 production in HT29-MTX cells and this capability was reinforced by the co-incubation with Lb. paraplantarum NB1 and Lb. rhamnosus GG. However, the increase in IL-8 production was not noticed when C difficile was co-incubated with EPS-producing Lb. paraplantarum BGCG11 strain or its purified EPS-CG11 polymer, thus indicating that the polymer could hinder the contact of bacteria with the intestinal epithelium. The measurement of mucus secreted by HT29-MTX and the expression of mud, muc2, muc3B and muc5AC genes in the presence of pathogens and lactobacilli suggested that all lactobacilli strains are weak "co-adjuvants" helping some pathogens to slightly increase the secretion of mucus by HT29-MTX, while purified EPS-CG11 did not induce mucus secretion. Taking altogether, Lb. paraplantarum BGCG11 could act towards the reinforcement of the innate mucosal barrier through the synthesis of a physical-protective EPS layer which could make difficult the contact of the pathogens with the epithelial cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX
EP  - 207
SP  - 199
VL  - 74
DO  - 10.1016/j.foodres.2015.05.012
ER  - 

@article{
author = "Živković, Milica and Hidalgo-Cantabrana, Claudio and Kojić, Milan and Gueimonde, Miguel and Golić, Nataša and Ruas-Madiedo, Patricia",
year = "2015",
abstract = "The putative protective role of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11, and its non-EPS-producing isogenic strain NB1, was tested upon HT29-MTX monolayers challenged with seven opportunistic pathogens. The probiotic strain Lactobacillus rhamnosus LMG18243 (GG) was used as a reference bacterium. Tested lactobacilli were able to efficiently reduce the attachment to HT29-MTX of most pathogens. Lb. paraplantarum NB1 and Lb. rhamnosus GG were more efficient reducing the adhesion of Clostridium difficile or Yersinia enterocolitica than Lb. paraplantarum BGCG11, while stain BGCG11 reduced, to a greater extent, the adhesion of Escherichia coli and Listeria monocytogenes. The detachment and cell lysis of HT29-MTX monolayers in the presence of pathogens alone and co-incubated with lactobacilli or purified EPS was followed. L. monocytogenes induced the strongest cell detachment among the seven tested pathogens and this effect was prevented by addition of purified EPS-CG11. The results suggest that this EPS could be an effective macromolecule in protection of HT29-MTX cells from the pathogen-induced lysis. Regarding innate intestinal barrier, the presence of C. difficile induced the highest IL-8 production in HT29-MTX cells and this capability was reinforced by the co-incubation with Lb. paraplantarum NB1 and Lb. rhamnosus GG. However, the increase in IL-8 production was not noticed when C difficile was co-incubated with EPS-producing Lb. paraplantarum BGCG11 strain or its purified EPS-CG11 polymer, thus indicating that the polymer could hinder the contact of bacteria with the intestinal epithelium. The measurement of mucus secreted by HT29-MTX and the expression of mud, muc2, muc3B and muc5AC genes in the presence of pathogens and lactobacilli suggested that all lactobacilli strains are weak "co-adjuvants" helping some pathogens to slightly increase the secretion of mucus by HT29-MTX, while purified EPS-CG11 did not induce mucus secretion. Taking altogether, Lb. paraplantarum BGCG11 could act towards the reinforcement of the innate mucosal barrier through the synthesis of a physical-protective EPS layer which could make difficult the contact of the pathogens with the epithelial cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX",
pages = "207-199",
volume = "74",
doi = "10.1016/j.foodres.2015.05.012"
}

Živković, M., Hidalgo-Cantabrana, C., Kojić, M., Gueimonde, M., Golić, N.,& Ruas-Madiedo, P.. (2015). Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. in Food Research International
Elsevier Science Bv, Amsterdam., 74, 199-207.
https://doi.org/10.1016/j.foodres.2015.05.012

Živković M, Hidalgo-Cantabrana C, Kojić M, Gueimonde M, Golić N, Ruas-Madiedo P. Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. in Food Research International. 2015;74:199-207.
doi:10.1016/j.foodres.2015.05.012 .

Živković, Milica, Hidalgo-Cantabrana, Claudio, Kojić, Milan, Gueimonde, Miguel, Golić, Nataša, Ruas-Madiedo, Patricia, "Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX" in Food Research International, 74 (2015):199-207,
https://doi.org/10.1016/j.foodres.2015.05.012 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Strahinić, Ivana
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Kojić, Snežana
AU  - Golić, Nataša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/805
AB  - Eleven Lactobacillus strains with strong aggregation abilities were selected from a laboratory collection. In two of the strains, genes associated with aggregation capability were plasmid located and found to strongly correlate with collagen binding. The gene encoding the auto-aggregation-promoting protein (AggLb) of Lactobacillus paracasei subsp. paracasei BGNJ1-64 was cloned using a novel, wide-range-host shuttle cloning vector, pAZILSJ. The clone pALb35, containing a 11377-bp DNA fragment, was selected from the SacI plasmid library for its ability to provide carriers with the aggregation phenotype. The complete fragment was sequenced and four potential ORFs were detected, including the aggLb gene and three surrounding transposase genes. AggLb is the largest known cell-surface protein in lactobacilli, consisting of 2998 aa (318,611 Da). AggLb belongs to the collagen-binding superfamily and its C-terminal region contains 20 successive repeats that are identical even at the nucleotide level. Deletion of aggLb causes a loss of the capacity to form cell aggregates, whereas overexpression increases cellular aggregation, hydrophobicity and collagen-binding potential. PCR screening performed with three sets of primers based on the aggLb gene of BGNJ1-64 enabled detection of the same type of aggLb gene in five of eleven selected aggregation-positive Lactobacillus strains. Heterologous expression of aggLb confirmed the crucial role of the AggLb protein in cell aggregation and specific collagen binding, indicating that AggLb has a useful probiotic function in effective colonization of host tissue and prevention of pathogen colonization.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro
IS  - 5
VL  - 10
DO  - 10.1371/journal.pone.0126387
ER  - 

@article{
author = "Miljković, Marija and Strahinić, Ivana and Tolinački, Maja and Živković, Milica and Kojić, Snežana and Golić, Nataša and Kojić, Milan",
year = "2015",
abstract = "Eleven Lactobacillus strains with strong aggregation abilities were selected from a laboratory collection. In two of the strains, genes associated with aggregation capability were plasmid located and found to strongly correlate with collagen binding. The gene encoding the auto-aggregation-promoting protein (AggLb) of Lactobacillus paracasei subsp. paracasei BGNJ1-64 was cloned using a novel, wide-range-host shuttle cloning vector, pAZILSJ. The clone pALb35, containing a 11377-bp DNA fragment, was selected from the SacI plasmid library for its ability to provide carriers with the aggregation phenotype. The complete fragment was sequenced and four potential ORFs were detected, including the aggLb gene and three surrounding transposase genes. AggLb is the largest known cell-surface protein in lactobacilli, consisting of 2998 aa (318,611 Da). AggLb belongs to the collagen-binding superfamily and its C-terminal region contains 20 successive repeats that are identical even at the nucleotide level. Deletion of aggLb causes a loss of the capacity to form cell aggregates, whereas overexpression increases cellular aggregation, hydrophobicity and collagen-binding potential. PCR screening performed with three sets of primers based on the aggLb gene of BGNJ1-64 enabled detection of the same type of aggLb gene in five of eleven selected aggregation-positive Lactobacillus strains. Heterologous expression of aggLb confirmed the crucial role of the AggLb protein in cell aggregation and specific collagen binding, indicating that AggLb has a useful probiotic function in effective colonization of host tissue and prevention of pathogen colonization.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro",
number = "5",
volume = "10",
doi = "10.1371/journal.pone.0126387"
}

Miljković, M., Strahinić, I., Tolinački, M., Živković, M., Kojić, S., Golić, N.,& Kojić, M.. (2015). AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro. in PLoS One
Public Library Science, San Francisco., 10(5).
https://doi.org/10.1371/journal.pone.0126387

Miljković M, Strahinić I, Tolinački M, Živković M, Kojić S, Golić N, Kojić M. AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro. in PLoS One. 2015;10(5).
doi:10.1371/journal.pone.0126387 .

Miljković, Marija, Strahinić, Ivana, Tolinački, Maja, Živković, Milica, Kojić, Snežana, Golić, Nataša, Kojić, Milan, "AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro" in PLoS One, 10, no. 5 (2015),
https://doi.org/10.1371/journal.pone.0126387 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Cadez, Neza
AU  - Uroić, Ksenija
AU  - Miljković, Marija
AU  - Tolinački, Maja
AU  - Dousova, Petra
AU  - Kos, Blazenka
AU  - Susković, Jagoda
AU  - Raspor, Peter
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/872
UR  - http://www.jocmr.com/fulltext/55-1410953223.pdf?1669198006
AB  - The aim of this study was to investigate the in vitro probiotic potential of dairy yeast isolates from artisanal cheeses manufactured in Serbia and Croatia.  
Methods. Twelve yeast strains isolated in from artisanal fresh soft and white brined cheeses manufactured in Serbia and Croatia were used in the study. Survival in chemically-simulated gastrointestinal conditions, adherence to epithelial intestinal cells and proliferation of gut-associated lymphoid tissue (GALT) cells were evaluated.
Results. The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit ( and #916; log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract. It was demonstrated that the strains Kluyvereomyces lactis ZIM 2408 and ZIM2441 and Saccharomyces cerevisiae ZIM 2415 were highly adhesive to Caco-2 cells, while strains Kluyvereomyces lactis ZIM 2408 and Debaryomyces hansenii ZIM 2415 exhibit the highest adhesion percentage to HT29-MTX cells. All strains significantly (p lt 0.0001) decreased the proliferation of gut-associated lymphoid tissue (GALT) cells suggesting the possible strain-specific immunomodulatory potential of the isolates. 
Conclusion. The dairy yeast isolates exhibit the strain-specific probiotic properties. Particularly, the strain K. lactis ZIM 2408 appears to be the best probiotic candidate in terms of all three criteria. Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.
T2  - Journal of complementary medicine research
T1  - Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia
EP  - 18
IS  - 1
SP  - 12
VL  - 4
DO  - 10.5455/jice.20141128051842
ER  - 

@article{
author = "Živković, Milica and Cadez, Neza and Uroić, Ksenija and Miljković, Marija and Tolinački, Maja and Dousova, Petra and Kos, Blazenka and Susković, Jagoda and Raspor, Peter and Topisirović, Ljubiša and Golić, Nataša",
year = "2015",
abstract = "The aim of this study was to investigate the in vitro probiotic potential of dairy yeast isolates from artisanal cheeses manufactured in Serbia and Croatia.  
Methods. Twelve yeast strains isolated in from artisanal fresh soft and white brined cheeses manufactured in Serbia and Croatia were used in the study. Survival in chemically-simulated gastrointestinal conditions, adherence to epithelial intestinal cells and proliferation of gut-associated lymphoid tissue (GALT) cells were evaluated.
Results. The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit ( and #916; log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract. It was demonstrated that the strains Kluyvereomyces lactis ZIM 2408 and ZIM2441 and Saccharomyces cerevisiae ZIM 2415 were highly adhesive to Caco-2 cells, while strains Kluyvereomyces lactis ZIM 2408 and Debaryomyces hansenii ZIM 2415 exhibit the highest adhesion percentage to HT29-MTX cells. All strains significantly (p lt 0.0001) decreased the proliferation of gut-associated lymphoid tissue (GALT) cells suggesting the possible strain-specific immunomodulatory potential of the isolates. 
Conclusion. The dairy yeast isolates exhibit the strain-specific probiotic properties. Particularly, the strain K. lactis ZIM 2408 appears to be the best probiotic candidate in terms of all three criteria. Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.",
journal = "Journal of complementary medicine research",
title = "Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia",
pages = "18-12",
number = "1",
volume = "4",
doi = "10.5455/jice.20141128051842"
}

Živković, M., Cadez, N., Uroić, K., Miljković, M., Tolinački, M., Dousova, P., Kos, B., Susković, J., Raspor, P., Topisirović, L.,& Golić, N.. (2015). Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. in Journal of complementary medicine research, 4(1), 12-18.
https://doi.org/10.5455/jice.20141128051842

Živković M, Cadez N, Uroić K, Miljković M, Tolinački M, Dousova P, Kos B, Susković J, Raspor P, Topisirović L, Golić N. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. in Journal of complementary medicine research. 2015;4(1):12-18.
doi:10.5455/jice.20141128051842 .

Živković, Milica, Cadez, Neza, Uroić, Ksenija, Miljković, Marija, Tolinački, Maja, Dousova, Petra, Kos, Blazenka, Susković, Jagoda, Raspor, Peter, Topisirović, Ljubiša, Golić, Nataša, "Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia" in Journal of complementary medicine research, 4, no. 1 (2015):12-18,
https://doi.org/10.5455/jice.20141128051842 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Živković, Milica
AU  - Tolinački, Maja
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/748
AB  - Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.
PB  - Springer, New York
T2  - Microbial Ecology
T1  - Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa
EP  - 644
IS  - 3
SP  - 633
VL  - 68
DO  - 10.1007/s00248-014-0426-1
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Živković, Milica and Tolinački, Maja and Kojić, Milan and Begović, Jelena",
year = "2014",
abstract = "Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.",
publisher = "Springer, New York",
journal = "Microbial Ecology",
title = "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa",
pages = "644-633",
number = "3",
volume = "68",
doi = "10.1007/s00248-014-0426-1"
}

Lukić, J., Strahinić, I., Milenković, M., Živković, M., Tolinački, M., Kojić, M.,& Begović, J.. (2014). Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology
Springer, New York., 68(3), 633-644.
https://doi.org/10.1007/s00248-014-0426-1

Lukić J, Strahinić I, Milenković M, Živković M, Tolinački M, Kojić M, Begović J. Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology. 2014;68(3):633-644.
doi:10.1007/s00248-014-0426-1 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Živković, Milica, Tolinački, Maja, Kojić, Milan, Begović, Jelena, "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa" in Microbial Ecology, 68, no. 3 (2014):633-644,
https://doi.org/10.1007/s00248-014-0426-1 . .

TY  - JOUR
AU  - Hidalgo-Cantabrana, Claudio
AU  - Živković, Milica
AU  - Lopez, Patricia
AU  - Suarez, Ana
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Margolles, Abelardo
AU  - Golić, Nataša
AU  - Ruas-Madiedo, Patricia
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/778
AB  - The effect of exopolysaccharide (EPS) producing bifidobacteria, and the EPS derived thereof, on the modulation of immune response was evaluated. Cells isolated from gut associated lymphoid tissue (GALT) and from peripheral blood mononuclear cells (PBMC) of naive rats were used. The proliferation and cytokine production of these immune cells in the presence of the three isogenic Bifidobacterium animalis subsp. lactis strains (A1, A1dOx and A1dOxR), as well as their purified polymers, were in vitro analysed. The cytokine pattern produced by immune cells isolated from GALT showed that most levels remained stable in the presence of the three strains or their corresponding polymers. However, in PBMC the UV-inactivated bacteria induced higher levels of the ratios IFN gamma/IL-17, TNF alpha/IL-10 and TNF alpha/TGF beta, and no variation in the ratio IFN gamma/IL-4. Thus, B. animalis subsp. lactis strains were able to activate blood monocytes as well as T lymphocytes towards a mild inflammatory Th1 response. Furthermore, only the EPS-A1dOxR was able to stimulate a response in a similar way than its EPS-producing bacterium. Our work supports the notion that some bifidobacterial EPS could play a role in mediating the dialog of these microorganisms with the immune system. In addition, this study emphasizes the effect that the origin of the immune cells has in results obtained; this could explain the great amount of contradiction found in literature about the immunomodulation capability of EPS from probiotic bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Anaerobe
T1  - Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue
EP  - 30
SP  - 24
VL  - 26
DO  - 10.1016/j.anaerobe.2014.01.003
ER  - 

@article{
author = "Hidalgo-Cantabrana, Claudio and Živković, Milica and Lopez, Patricia and Suarez, Ana and Miljković, Marija and Kojić, Milan and Margolles, Abelardo and Golić, Nataša and Ruas-Madiedo, Patricia",
year = "2014",
abstract = "The effect of exopolysaccharide (EPS) producing bifidobacteria, and the EPS derived thereof, on the modulation of immune response was evaluated. Cells isolated from gut associated lymphoid tissue (GALT) and from peripheral blood mononuclear cells (PBMC) of naive rats were used. The proliferation and cytokine production of these immune cells in the presence of the three isogenic Bifidobacterium animalis subsp. lactis strains (A1, A1dOx and A1dOxR), as well as their purified polymers, were in vitro analysed. The cytokine pattern produced by immune cells isolated from GALT showed that most levels remained stable in the presence of the three strains or their corresponding polymers. However, in PBMC the UV-inactivated bacteria induced higher levels of the ratios IFN gamma/IL-17, TNF alpha/IL-10 and TNF alpha/TGF beta, and no variation in the ratio IFN gamma/IL-4. Thus, B. animalis subsp. lactis strains were able to activate blood monocytes as well as T lymphocytes towards a mild inflammatory Th1 response. Furthermore, only the EPS-A1dOxR was able to stimulate a response in a similar way than its EPS-producing bacterium. Our work supports the notion that some bifidobacterial EPS could play a role in mediating the dialog of these microorganisms with the immune system. In addition, this study emphasizes the effect that the origin of the immune cells has in results obtained; this could explain the great amount of contradiction found in literature about the immunomodulation capability of EPS from probiotic bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Anaerobe",
title = "Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue",
pages = "30-24",
volume = "26",
doi = "10.1016/j.anaerobe.2014.01.003"
}

Hidalgo-Cantabrana, C., Živković, M., Lopez, P., Suarez, A., Miljković, M., Kojić, M., Margolles, A., Golić, N.,& Ruas-Madiedo, P.. (2014). Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue. in Anaerobe
Elsevier Sci Ltd, Oxford., 26, 24-30.
https://doi.org/10.1016/j.anaerobe.2014.01.003

Hidalgo-Cantabrana C, Živković M, Lopez P, Suarez A, Miljković M, Kojić M, Margolles A, Golić N, Ruas-Madiedo P. Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue. in Anaerobe. 2014;26:24-30.
doi:10.1016/j.anaerobe.2014.01.003 .

Hidalgo-Cantabrana, Claudio, Živković, Milica, Lopez, Patricia, Suarez, Ana, Miljković, Marija, Kojić, Milan, Margolles, Abelardo, Golić, Nataša, Ruas-Madiedo, Patricia, "Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue" in Anaerobe, 26 (2014):24-30,
https://doi.org/10.1016/j.anaerobe.2014.01.003 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Mihajlović, Sanja
AU  - Uzelac, Gordana
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/791
AB  - The aim of this study was to investigate the composition of lactic acid bacteria (LAB) in autochthonous young cheeses, sweet creams and sweet kajmaks produced in the Vlasic mountain region of central Bosnia and Herzegovina near the town of Travnik over a four season period. These three products were made from cow's milk by a traditional method without the addition of a starter culture. Preliminary characterization with phenotype-based assays and identification using rep-PCR with a (GTG)(5) primer and 16S rDNA sequence analysis were undertaken for 460 LAB isolates obtained from all the examined samples. Fifteen species were identified as follows: Lactococcus lactis, Lactococcus raffinolactis, Lactococcus garviae, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus helveticus, Enterococcus faecium, Enterococcus durans, Enterococcus faecalis, Enterococcus italicus, Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc lactis, Streptococcus thermophilus and Streptococcus mitis. A wide genotypic and phenotypic heterogeneity of the species was observed, particularly within the Lc. lactis strains. In all of the tested dairy products across four seasons, a significantly positive correlation (r = 0.690) between the presence of lactococci and enterococci and a negative correlation (r = 0.722) between the presence of lactococci and leuconostocs were recorded. Forty-five percent of the lactobacilli and 54.4% of the lactococci exhibited proteolytic activity, whereas 18.7% of the total LAB isolates exhibited antimicrobial activity.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Food Microbiology
T1  - Characterization of lactic acid bacteria isolated from artisanal Travnik young cheeses, sweet creams and sweet kajmaks over four seasons
EP  - 38
SP  - 27
VL  - 39
DO  - 10.1016/j.fm.2013.10.011
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Mihajlović, Sanja and Uzelac, Gordana and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Topisirović, Ljubiša and Kojić, Milan",
year = "2014",
abstract = "The aim of this study was to investigate the composition of lactic acid bacteria (LAB) in autochthonous young cheeses, sweet creams and sweet kajmaks produced in the Vlasic mountain region of central Bosnia and Herzegovina near the town of Travnik over a four season period. These three products were made from cow's milk by a traditional method without the addition of a starter culture. Preliminary characterization with phenotype-based assays and identification using rep-PCR with a (GTG)(5) primer and 16S rDNA sequence analysis were undertaken for 460 LAB isolates obtained from all the examined samples. Fifteen species were identified as follows: Lactococcus lactis, Lactococcus raffinolactis, Lactococcus garviae, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus helveticus, Enterococcus faecium, Enterococcus durans, Enterococcus faecalis, Enterococcus italicus, Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Leuconostoc lactis, Streptococcus thermophilus and Streptococcus mitis. A wide genotypic and phenotypic heterogeneity of the species was observed, particularly within the Lc. lactis strains. In all of the tested dairy products across four seasons, a significantly positive correlation (r = 0.690) between the presence of lactococci and enterococci and a negative correlation (r = 0.722) between the presence of lactococci and leuconostocs were recorded. Forty-five percent of the lactobacilli and 54.4% of the lactococci exhibited proteolytic activity, whereas 18.7% of the total LAB isolates exhibited antimicrobial activity.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Food Microbiology",
title = "Characterization of lactic acid bacteria isolated from artisanal Travnik young cheeses, sweet creams and sweet kajmaks over four seasons",
pages = "38-27",
volume = "39",
doi = "10.1016/j.fm.2013.10.011"
}

Terzić-Vidojević, A., Mihajlović, S., Uzelac, G., Veljović, K., Tolinački, M., Živković, M., Topisirović, L.,& Kojić, M.. (2014). Characterization of lactic acid bacteria isolated from artisanal Travnik young cheeses, sweet creams and sweet kajmaks over four seasons. in Food Microbiology
Academic Press Ltd- Elsevier Science Ltd, London., 39, 27-38.
https://doi.org/10.1016/j.fm.2013.10.011

Terzić-Vidojević A, Mihajlović S, Uzelac G, Veljović K, Tolinački M, Živković M, Topisirović L, Kojić M. Characterization of lactic acid bacteria isolated from artisanal Travnik young cheeses, sweet creams and sweet kajmaks over four seasons. in Food Microbiology. 2014;39:27-38.
doi:10.1016/j.fm.2013.10.011 .

Terzić-Vidojević, Amarela, Mihajlović, Sanja, Uzelac, Gordana, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Topisirović, Ljubiša, Kojić, Milan, "Characterization of lactic acid bacteria isolated from artisanal Travnik young cheeses, sweet creams and sweet kajmaks over four seasons" in Food Microbiology, 39 (2014):27-38,
https://doi.org/10.1016/j.fm.2013.10.011 . .

TY  - JOUR
AU  - Uroić, Ksenija
AU  - Živković, Milica
AU  - Kos, Blazenka
AU  - Pavunc, Andreja Lebos
AU  - Beganović, Jasna
AU  - Lukić, Jovanka
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Miljković, Marija
AU  - Golić, Nataša
AU  - Topisirović, Ljubiša
AU  - Cadez, Neza
AU  - Raspor, Peter
AU  - Susković, Jagoda
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/760
AB  - The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese
EP  - 241
IS  - 2
SP  - 232
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_imagine_760
ER  - 

@article{
author = "Uroić, Ksenija and Živković, Milica and Kos, Blazenka and Pavunc, Andreja Lebos and Beganović, Jasna and Lukić, Jovanka and Jovčić, Branko and Filipić, Brankica and Miljković, Marija and Golić, Nataša and Topisirović, Ljubiša and Cadez, Neza and Raspor, Peter and Susković, Jagoda",
year = "2014",
abstract = "The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese",
pages = "241-232",
number = "2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_imagine_760"
}

Uroić, K., Živković, M., Kos, B., Pavunc, A. L., Beganović, J., Lukić, J., Jovčić, B., Filipić, B., Miljković, M., Golić, N., Topisirović, L., Cadez, N., Raspor, P.,& Susković, J.. (2014). Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology
University of Zagreb., 52(2), 232-241.
https://hdl.handle.net/21.15107/rcub_imagine_760

Uroić K, Živković M, Kos B, Pavunc AL, Beganović J, Lukić J, Jovčić B, Filipić B, Miljković M, Golić N, Topisirović L, Cadez N, Raspor P, Susković J. Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology. 2014;52(2):232-241.
https://hdl.handle.net/21.15107/rcub_imagine_760 .

Uroić, Ksenija, Živković, Milica, Kos, Blazenka, Pavunc, Andreja Lebos, Beganović, Jasna, Lukić, Jovanka, Jovčić, Branko, Filipić, Brankica, Miljković, Marija, Golić, Nataša, Topisirović, Ljubiša, Cadez, Neza, Raspor, Peter, Susković, Jagoda, "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese" in Food Technology and Biotechnology, 52, no. 2 (2014):232-241,
https://hdl.handle.net/21.15107/rcub_imagine_760 .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Veljović, Katarina
AU  - Jovanović, Snežana
AU  - Macej, Ognjen
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/696
AB  - The aim of this study is the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheese for the selection of potential starter cultures. Soft white Vlasina cheese was manufactured at a household on the Stara Planina Mountain using traditional techniques without starter cultures. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheese, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in the cheese samples was characterized by phenotype-based assays and presumptively identified using repetitive element palindromic polymerase chain reaction (rep-PCR) with the primer (GTG)(5). Results were confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15-day-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis ssp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exo-polysaccharides, while BGVL2-29 and BGVL2-63, which belonged to the species Ln. pseudomesenteroides, utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in the starter culture. This study contributed to the understanding of the role of autochthonous LAB in the quality of artisanal cheese and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures
EP  - 563
IS  - 4
SP  - 554
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_imagine_696
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Tolinački, Maja and Živković, Milica and Veljović, Katarina and Jovanović, Snežana and Macej, Ognjen and Topisirović, Ljubiša",
year = "2013",
abstract = "The aim of this study is the isolation, characterization and identification of autochthonous lactic acid bacteria (LAB) from artisanal Vlasina raw goat's milk cheese for the selection of potential starter cultures. Soft white Vlasina cheese was manufactured at a household on the Stara Planina Mountain using traditional techniques without starter cultures. One hundred and forty nine LAB isolates were collected from two samples of Vlasina cheese, designated as BGVL2 (5 days old) and BGVL2a (15 days old). The population of LAB in the cheese samples was characterized by phenotype-based assays and presumptively identified using repetitive element palindromic polymerase chain reaction (rep-PCR) with the primer (GTG)(5). Results were confirmed by 16S rDNA sequencing. Among the BGVL2 isolates (56), the most numerous LAB species were Leuconostoc pseudomesenteroides (27) and Lactococcus lactis (26). In 15-day-old BGVL2a (93 isolates), Lactobacillus plantarum (33), Enterococcus durans (26) and Pediococcus pentosaceus (14) were predominant. Lc. lactis ssp. lactis BGVL2-8 showed good acidification ability and the ability to produce antimicrobial compounds, Lb. plantarum BGVL2a-18 had good proteolytic ability and produced exo-polysaccharides, while BGVL2-29 and BGVL2-63, which belonged to the species Ln. pseudomesenteroides, utilized citrate and produced diacetyl and acetoin. They appeared to be suitable candidates for inclusion in the starter culture. This study contributed to the understanding of the role of autochthonous LAB in the quality of artisanal cheese and the possibility of using the selected LAB as potential starter cultures for cheese making under controlled conditions.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures",
pages = "563-554",
number = "4",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_imagine_696"
}

Terzić-Vidojević, A., Tolinački, M., Živković, M., Veljović, K., Jovanović, S., Macej, O.,& Topisirović, L.. (2013). Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 51(4), 554-563.
https://hdl.handle.net/21.15107/rcub_imagine_696

Terzić-Vidojević A, Tolinački M, Živković M, Veljović K, Jovanović S, Macej O, Topisirović L. Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures. in Food Technology and Biotechnology. 2013;51(4):554-563.
https://hdl.handle.net/21.15107/rcub_imagine_696 .

Terzić-Vidojević, Amarela, Tolinački, Maja, Živković, Milica, Veljović, Katarina, Jovanović, Snežana, Macej, Ognjen, Topisirović, Ljubiša, "Artisanal Vlasina Raw Goat's Milk Cheese: Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures" in Food Technology and Biotechnology, 51, no. 4 (2013):554-563,
https://hdl.handle.net/21.15107/rcub_imagine_696 .

TY  - JOUR
AU  - Živković, Milica
AU  - Lopez, Patricia
AU  - Strahinić, Ivana
AU  - Suarez, Ana
AU  - Kojić, Milan
AU  - Fernandez-Garcia, Maria
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
AU  - Ruas-Madiedo, Patricia
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/568
AB  - Traditional fermented foods are the best source for the isolation of strains with specific traits to act as functional starters and to keep the biodiversity of the culture collections. Besides, these strains could be used in the formulation of foods claimed to promote health benefits, i.e. those containing probiotic microorganisms. For the rational selection of strains acting as probiotics, several in vitro tests have been proposed. In the current study, we have characterized the probiotic potential of the strain Lactobacillus paraplanta rum BGCG11, isolated from a Serbian soft, white, homemade cheese, which is able to produce a "ropy" exopolysaccharide (EPS). Three novobiocin derivative strains, which have lost the ropy phenotype, were characterized as well in order to determine the putative role of the EPS in the probiotic potential. Under chemically gastrointestinal conditions, all strains were able to survive around 1-2% (10(6)-10(7) cfu/ml cultivable bacteria) only when they were included in a food matrix (1% skimmed milk). The strains were more resistant to acid conditions than to bile salts and gastric or pancreatic enzymes, which could be due to a pre-adaptation of the parental strain to acidic conditions in the cheese habitat. The ropy EPS did not improve the survival of the producing strain. On the contrary, the presence of an EPS layer surrounding the strain BGCG11 hindered its adhesion to the three epithelial intestinal cell lines tested, since the adhesion of the three non-ropy derivatives was higher than the parental one and also than that of the reference strain Lactobacillus rhamnosus CC. Aiming to propose a potential target application of these strains as probiotics, the cytokine production of peripheral blood mononuclear cells (PBMC) was analyzed. The EPS-producing L paraplantarum BGCG11 strain showed an anti-inflammatory or immunosuppressor profile whereas the non-ropy derivative strains induced higher pro-inflammatory response. In addition, when PBMC were stimulated with increasing concentrations of the purified ropy EPS (1, 10 and 100 mu g/ml) the cytokine profile was similar to that obtained with the EPS-producing lactobacilli, therefore pointing to a putative role of this biopolymer in its immune response.
PB  - Elsevier, Amsterdam
T2  - International Journal of Food Microbiology
T1  - Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics
EP  - 162
IS  - 2
SP  - 155
VL  - 158
DO  - 10.1016/j.ijfoodmicro.2012.07.015
ER  - 

@article{
author = "Živković, Milica and Lopez, Patricia and Strahinić, Ivana and Suarez, Ana and Kojić, Milan and Fernandez-Garcia, Maria and Topisirović, Ljubiša and Golić, Nataša and Ruas-Madiedo, Patricia",
year = "2012",
abstract = "Traditional fermented foods are the best source for the isolation of strains with specific traits to act as functional starters and to keep the biodiversity of the culture collections. Besides, these strains could be used in the formulation of foods claimed to promote health benefits, i.e. those containing probiotic microorganisms. For the rational selection of strains acting as probiotics, several in vitro tests have been proposed. In the current study, we have characterized the probiotic potential of the strain Lactobacillus paraplanta rum BGCG11, isolated from a Serbian soft, white, homemade cheese, which is able to produce a "ropy" exopolysaccharide (EPS). Three novobiocin derivative strains, which have lost the ropy phenotype, were characterized as well in order to determine the putative role of the EPS in the probiotic potential. Under chemically gastrointestinal conditions, all strains were able to survive around 1-2% (10(6)-10(7) cfu/ml cultivable bacteria) only when they were included in a food matrix (1% skimmed milk). The strains were more resistant to acid conditions than to bile salts and gastric or pancreatic enzymes, which could be due to a pre-adaptation of the parental strain to acidic conditions in the cheese habitat. The ropy EPS did not improve the survival of the producing strain. On the contrary, the presence of an EPS layer surrounding the strain BGCG11 hindered its adhesion to the three epithelial intestinal cell lines tested, since the adhesion of the three non-ropy derivatives was higher than the parental one and also than that of the reference strain Lactobacillus rhamnosus CC. Aiming to propose a potential target application of these strains as probiotics, the cytokine production of peripheral blood mononuclear cells (PBMC) was analyzed. The EPS-producing L paraplantarum BGCG11 strain showed an anti-inflammatory or immunosuppressor profile whereas the non-ropy derivative strains induced higher pro-inflammatory response. In addition, when PBMC were stimulated with increasing concentrations of the purified ropy EPS (1, 10 and 100 mu g/ml) the cytokine profile was similar to that obtained with the EPS-producing lactobacilli, therefore pointing to a putative role of this biopolymer in its immune response.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics",
pages = "162-155",
number = "2",
volume = "158",
doi = "10.1016/j.ijfoodmicro.2012.07.015"
}

Živković, M., Lopez, P., Strahinić, I., Suarez, A., Kojić, M., Fernandez-Garcia, M., Topisirović, L., Golić, N.,& Ruas-Madiedo, P.. (2012). Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. in International Journal of Food Microbiology
Elsevier, Amsterdam., 158(2), 155-162.
https://doi.org/10.1016/j.ijfoodmicro.2012.07.015

Živković M, Lopez P, Strahinić I, Suarez A, Kojić M, Fernandez-Garcia M, Topisirović L, Golić N, Ruas-Madiedo P. Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. in International Journal of Food Microbiology. 2012;158(2):155-162.
doi:10.1016/j.ijfoodmicro.2012.07.015 .

Živković, Milica, Lopez, Patricia, Strahinić, Ivana, Suarez, Ana, Kojić, Milan, Fernandez-Garcia, Maria, Topisirović, Ljubiša, Golić, Nataša, Ruas-Madiedo, Patricia, "Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics" in International Journal of Food Microbiology, 158, no. 2 (2012):155-162,
https://doi.org/10.1016/j.ijfoodmicro.2012.07.015 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Strahinić, Ivana
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Živković, Milica
AU  - Tolinački, Maja
AU  - Topisirović, Ljubiša
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/597
AB  - The diversity of 87 Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains, previously identified from different autochthonous dairy products, was investigated by phenotypic and genotypic approaches. The increased resolution obtained using phenotypic and genotypic characterization allowed the level of strain heterogeneity detection to be widened. Phenotypic diversity was evaluated by studying biochemical characteristics of technological interest, including antimicrobial and proteinase activities, resistance to nisin, aggregation ability, production of exopolysaccharides, acetoin and diacetyl, citrate utilization, and antibiotic susceptibility. Genotypic diversity was generally evaluated by PCR amplification of repetitive bacterial DNA element fingerprinting using the (GTG)(5) primer [(GTG)(5)-PCR]. Moreover, in cases where strains were not discriminated by (GTG)(5)-PCR combined with phenotypic analysis, pulsed-field gel electrophoresis (PFGE) analysis was performed. The results indicate that L. plantarum/paraplantarum and L. paracasei natural isolates from artisanal dairy products are a gold mine in terms of diversity of strains and could be potentially interesting to dairy companies for the formulation of functional starter cultures in the production of innovative foods.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products
EP  - 638
IS  - 4
SP  - 627
VL  - 234
DO  - 10.1007/s00217-012-1670-6
ER  - 

@article{
author = "Golić, Nataša and Strahinić, Ivana and Terzić-Vidojević, Amarela and Begović, Jelena and Živković, Milica and Tolinački, Maja and Topisirović, Ljubiša",
year = "2012",
abstract = "The diversity of 87 Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains, previously identified from different autochthonous dairy products, was investigated by phenotypic and genotypic approaches. The increased resolution obtained using phenotypic and genotypic characterization allowed the level of strain heterogeneity detection to be widened. Phenotypic diversity was evaluated by studying biochemical characteristics of technological interest, including antimicrobial and proteinase activities, resistance to nisin, aggregation ability, production of exopolysaccharides, acetoin and diacetyl, citrate utilization, and antibiotic susceptibility. Genotypic diversity was generally evaluated by PCR amplification of repetitive bacterial DNA element fingerprinting using the (GTG)(5) primer [(GTG)(5)-PCR]. Moreover, in cases where strains were not discriminated by (GTG)(5)-PCR combined with phenotypic analysis, pulsed-field gel electrophoresis (PFGE) analysis was performed. The results indicate that L. plantarum/paraplantarum and L. paracasei natural isolates from artisanal dairy products are a gold mine in terms of diversity of strains and could be potentially interesting to dairy companies for the formulation of functional starter cultures in the production of innovative foods.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products",
pages = "638-627",
number = "4",
volume = "234",
doi = "10.1007/s00217-012-1670-6"
}

Golić, N., Strahinić, I., Terzić-Vidojević, A., Begović, J., Živković, M., Tolinački, M.,& Topisirović, L.. (2012). Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products. in European Food Research and Technology
Springer, New York., 234(4), 627-638.
https://doi.org/10.1007/s00217-012-1670-6

Golić N, Strahinić I, Terzić-Vidojević A, Begović J, Živković M, Tolinački M, Topisirović L. Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products. in European Food Research and Technology. 2012;234(4):627-638.
doi:10.1007/s00217-012-1670-6 .

Golić, Nataša, Strahinić, Ivana, Terzić-Vidojević, Amarela, Begović, Jelena, Živković, Milica, Tolinački, Maja, Topisirović, Ljubiša, "Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products" in European Food Research and Technology, 234, no. 4 (2012):627-638,
https://doi.org/10.1007/s00217-012-1670-6 . .