TY  - JOUR
AU  - Schneider, Olha
AU  - Ilić-Tomić, Tatjana
AU  - Rueckert, Christian
AU  - Kalinowski, Joern
AU  - Gencić, Marija S.
AU  - Zivković, Milena Z.
AU  - Stanković, Nada
AU  - Radulović, Niko S.
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Zotchev, Sergey B.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1107
AB  - Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10
VL  - 9
DO  - 10.3389/fmicb.2018.01302
ER  - 

@article{
author = "Schneider, Olha and Ilić-Tomić, Tatjana and Rueckert, Christian and Kalinowski, Joern and Gencić, Marija S. and Zivković, Milena Z. and Stanković, Nada and Radulović, Niko S. and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Zotchev, Sergey B.",
year = "2018",
abstract = "Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10",
volume = "9",
doi = "10.3389/fmicb.2018.01302"
}

Schneider, O., Ilić-Tomić, T., Rueckert, C., Kalinowski, J., Gencić, M. S., Zivković, M. Z., Stanković, N., Radulović, N. S., Vasiljević, B., Nikodinović-Runić, J.,& Zotchev, S. B.. (2018). Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.01302

Schneider O, Ilić-Tomić T, Rueckert C, Kalinowski J, Gencić MS, Zivković MZ, Stanković N, Radulović NS, Vasiljević B, Nikodinović-Runić J, Zotchev SB. Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.01302 .

Schneider, Olha, Ilić-Tomić, Tatjana, Rueckert, Christian, Kalinowski, Joern, Gencić, Marija S., Zivković, Milena Z., Stanković, Nada, Radulović, Niko S., Vasiljević, Branka, Nikodinović-Runić, Jasmina, Zotchev, Sergey B., "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.01302 . .

TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Gencić, Marija S.
AU  - Zivković, Milena Z.
AU  - Vasiljević, Branka
AU  - Đokić, Lidija
AU  - Nikodinović-Runić, Jasmina
AU  - Radulović, Niko S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/875
AB  - Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10
EP  - 4833
IS  - 11
SP  - 4815
VL  - 99
DO  - 10.1007/s00253-014-6364-5
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Gencić, Marija S. and Zivković, Milena Z. and Vasiljević, Branka and Đokić, Lidija and Nikodinović-Runić, Jasmina and Radulović, Niko S.",
year = "2015",
abstract = "Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 A degrees C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C-7-C-30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10",
pages = "4833-4815",
number = "11",
volume = "99",
doi = "10.1007/s00253-014-6364-5"
}

Ilić-Tomić, T., Gencić, M. S., Zivković, M. Z., Vasiljević, B., Đokić, L., Nikodinović-Runić, J.,& Radulović, N. S.. (2015). Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology
Springer, New York., 99(11), 4815-4833.
https://doi.org/10.1007/s00253-014-6364-5

Ilić-Tomić T, Gencić MS, Zivković MZ, Vasiljević B, Đokić L, Nikodinović-Runić J, Radulović NS. Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10. in Applied Microbiology and Biotechnology. 2015;99(11):4815-4833.
doi:10.1007/s00253-014-6364-5 .

Ilić-Tomić, Tatjana, Gencić, Marija S., Zivković, Milena Z., Vasiljević, Branka, Đokić, Lidija, Nikodinović-Runić, Jasmina, Radulović, Niko S., "Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp NP10" in Applied Microbiology and Biotechnology, 99, no. 11 (2015):4815-4833,
https://doi.org/10.1007/s00253-014-6364-5 . .