TY  - THES
AU  - Mandić, Mina
PY  - 2021
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8478
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24879/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=52766473
UR  - https://nardus.mpn.gov.rs/handle/123456789/18902
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/64
AB  - Bakterije roda Pseudomonas imaju sposobnost produkcije i razgradnje širokog spektra jedinjenja. Cilj ove doktorske teze bio je da se u kolekciji sojeva roda Pseudomonas identifikuju i rekombinantno eksprimiraju nove lakaze i lipaze, i evaluira njihov aplikativni potencijal. Sedam sojeva obuhvaćeno je analizom oksidativne i hidrolitičke aktivnosti prema pet različitih supstrata. Kod sojeva Pseudomonas putida F6, P. putida KT2440 i P. putida CA-3, utvrđeno je prisustvo gena koji kodiraju lakaze. Proteini McoCA3, McoKT, Cbp i CopA heterologo su eksprimirani u E. coli domaćinu, prečišćeni i okarakterisani. Pokazana je njihova aktivnost u širokom pH i temperaturnom opsegu kao i visoka termostabilnost. Ispitana je i sposobnost enzima da razgrade boje koje se koriste u tekstilnoj industriji, potencijalne zagađivače voda. Ćelijski ekstrakt koji je sadržao CopA enzim iz P. putida F6 pokazao je najznačajniju aktivnost razgradivši pet od sedam testiranih boja. Kod sojeva P. aeruginosa PAO1 i P. chlororaphis B-561, detektovana je lipolitička aktivnost i utvrđen potencijal za biorazgradnju polimera kao što su polihidroksialkanoati srednjeg lanca i polikaprolakton, a degradacioni potencijal soja B-561 potvrđen je i u modelu komposta. Homologo su eksprimirana tri proteina – LipA, PlcB i LipA, ali je zaključeno da nije došlo do njihove sekrecije. U ovom radu je pokazano da su Pseudomonas sojevi dobar izvor biotehnološki značajnih enzima lakaza i lipaza. Rekombinantna ekspresija lakaza dala je funkcionalne enzime pogodne za primenu u razgradnji sintetičkih boja iz otpadnih voda. Za primenu rekombinantnih lipaza u biorazgradnji polimernih materijala neophodna je optimizacija sekrecije, ali divlji sojevi koji eksprimiraju ove enzime su dovoljno dobri biokatalizatori za njihovu razgradnju.
AB  - Pseudomonas strains have the ability to produce and degrade a wide range of compounds. The main objective of this thesis was to identify, recombinantly express and characterize novel laccases and lipases from Pseudomonas spp. collection, and to evaluate their application potential. Seven strains were analyzed for oxidative and hydrolytic activity towards five different substrates. Genes encoding laccases were detected in P. putida F6, P. putida KT2440 and P. putida CA-3 strains. Proteins McoCA3, McoKT, Cbp and CopA were heterologously expressed in E. coli, purified and characterized, exhibiting broad temperature and pH range and high thermal stability. The ability of enzymes to degrade textile dyes was also examined. All enzymes proved to be very efficient in degradation of synthetic dyes, with the CopA enzyme from P. putida F6 showing the most significant activity, degrading five out of seven tested dyes. Lipolytic activity was detected in two strains: P. aeruginosa PAO1 and P. chlororaphis B-561. The potential for biodegradation of polymeric materials such as medium chain length polyhydroxyalkanoates and polycaprolactone was demonstrated, while degradation potential of B-561 was confirmed in the compost system as well. Three lipases were homologously expressed – LipA, PlcB and LipA, but they were not secreted. This study showed that Pseudomonas genus is a good source of biotechnologically relevant enzymes. Recombinant laccase expression yielded enzymes suitable for application in the degradation of persistent synthetic dyes from wastewaters. For the application of recombinant lipases in the biodegradation of polymers, optimization of the secretion is necessary, however wild type strains expressing these enzymes are good enough biocatalysts for biodegradation purpose.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza
T1  - Biotechnologically relevant enzymes in strains of Pseudomonas genus: Identification and recombinant expression of laccases and lipases
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18902
ER  - 

@phdthesis{
author = "Mandić, Mina",
year = "2021",
abstract = "Bakterije roda Pseudomonas imaju sposobnost produkcije i razgradnje širokog spektra jedinjenja. Cilj ove doktorske teze bio je da se u kolekciji sojeva roda Pseudomonas identifikuju i rekombinantno eksprimiraju nove lakaze i lipaze, i evaluira njihov aplikativni potencijal. Sedam sojeva obuhvaćeno je analizom oksidativne i hidrolitičke aktivnosti prema pet različitih supstrata. Kod sojeva Pseudomonas putida F6, P. putida KT2440 i P. putida CA-3, utvrđeno je prisustvo gena koji kodiraju lakaze. Proteini McoCA3, McoKT, Cbp i CopA heterologo su eksprimirani u E. coli domaćinu, prečišćeni i okarakterisani. Pokazana je njihova aktivnost u širokom pH i temperaturnom opsegu kao i visoka termostabilnost. Ispitana je i sposobnost enzima da razgrade boje koje se koriste u tekstilnoj industriji, potencijalne zagađivače voda. Ćelijski ekstrakt koji je sadržao CopA enzim iz P. putida F6 pokazao je najznačajniju aktivnost razgradivši pet od sedam testiranih boja. Kod sojeva P. aeruginosa PAO1 i P. chlororaphis B-561, detektovana je lipolitička aktivnost i utvrđen potencijal za biorazgradnju polimera kao što su polihidroksialkanoati srednjeg lanca i polikaprolakton, a degradacioni potencijal soja B-561 potvrđen je i u modelu komposta. Homologo su eksprimirana tri proteina – LipA, PlcB i LipA, ali je zaključeno da nije došlo do njihove sekrecije. U ovom radu je pokazano da su Pseudomonas sojevi dobar izvor biotehnološki značajnih enzima lakaza i lipaza. Rekombinantna ekspresija lakaza dala je funkcionalne enzime pogodne za primenu u razgradnji sintetičkih boja iz otpadnih voda. Za primenu rekombinantnih lipaza u biorazgradnji polimernih materijala neophodna je optimizacija sekrecije, ali divlji sojevi koji eksprimiraju ove enzime su dovoljno dobri biokatalizatori za njihovu razgradnju., Pseudomonas strains have the ability to produce and degrade a wide range of compounds. The main objective of this thesis was to identify, recombinantly express and characterize novel laccases and lipases from Pseudomonas spp. collection, and to evaluate their application potential. Seven strains were analyzed for oxidative and hydrolytic activity towards five different substrates. Genes encoding laccases were detected in P. putida F6, P. putida KT2440 and P. putida CA-3 strains. Proteins McoCA3, McoKT, Cbp and CopA were heterologously expressed in E. coli, purified and characterized, exhibiting broad temperature and pH range and high thermal stability. The ability of enzymes to degrade textile dyes was also examined. All enzymes proved to be very efficient in degradation of synthetic dyes, with the CopA enzyme from P. putida F6 showing the most significant activity, degrading five out of seven tested dyes. Lipolytic activity was detected in two strains: P. aeruginosa PAO1 and P. chlororaphis B-561. The potential for biodegradation of polymeric materials such as medium chain length polyhydroxyalkanoates and polycaprolactone was demonstrated, while degradation potential of B-561 was confirmed in the compost system as well. Three lipases were homologously expressed – LipA, PlcB and LipA, but they were not secreted. This study showed that Pseudomonas genus is a good source of biotechnologically relevant enzymes. Recombinant laccase expression yielded enzymes suitable for application in the degradation of persistent synthetic dyes from wastewaters. For the application of recombinant lipases in the biodegradation of polymers, optimization of the secretion is necessary, however wild type strains expressing these enzymes are good enough biocatalysts for biodegradation purpose.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza, Biotechnologically relevant enzymes in strains of Pseudomonas genus: Identification and recombinant expression of laccases and lipases",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18902"
}

Mandić, M.. (2021). Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_18902

Mandić M. Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18902 .

Mandić, Mina, "Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza" (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18902 .

TY  - JOUR
AU  - Mandić, Mina
AU  - Đokić, Lidija
AU  - Nikolaivits, Efstratios
AU  - Prodanović, Radivoje
AU  - O'Connor, Kevin
AU  - Jeremić, Sanja
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1284
AB  - Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.
PB  - MDPI, Basel
T2  - Catalysts
T1  - Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes
IS  - 7
VL  - 9
DO  - 10.3390/catal9070629
ER  - 

@article{
author = "Mandić, Mina and Đokić, Lidija and Nikolaivits, Efstratios and Prodanović, Radivoje and O'Connor, Kevin and Jeremić, Sanja and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2019",
abstract = "Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.",
publisher = "MDPI, Basel",
journal = "Catalysts",
title = "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes",
number = "7",
volume = "9",
doi = "10.3390/catal9070629"
}

Mandić, M., Đokić, L., Nikolaivits, E., Prodanović, R., O'Connor, K., Jeremić, S., Topakas, E.,& Nikodinović-Runić, J.. (2019). Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts
MDPI, Basel., 9(7).
https://doi.org/10.3390/catal9070629

Mandić M, Đokić L, Nikolaivits E, Prodanović R, O'Connor K, Jeremić S, Topakas E, Nikodinović-Runić J. Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts. 2019;9(7).
doi:10.3390/catal9070629 .

Mandić, Mina, Đokić, Lidija, Nikolaivits, Efstratios, Prodanović, Radivoje, O'Connor, Kevin, Jeremić, Sanja, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes" in Catalysts, 9, no. 7 (2019),
https://doi.org/10.3390/catal9070629 . .

TY  - JOUR
AU  - Mandić, Mina
AU  - Spasić, Jelena
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija S.
AU  - Cosović, Vladan R.
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
AU  - Jeremić, Sanja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1760
AB  - Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Degradation and Stability
T1  - Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil
EP  - 168
SP  - 160
VL  - 162
DO  - 10.1016/j.polymdegradstab.2019.02.012
ER  - 

@article{
author = "Mandić, Mina and Spasić, Jelena and Ponjavić, Marijana and Nikolić, Marija S. and Cosović, Vladan R. and O'Connor, Kevin and Nikodinović-Runić, Jasmina and Đokić, Lidija and Jeremić, Sanja",
year = "2019",
abstract = "Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Degradation and Stability",
title = "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil",
pages = "168-160",
volume = "162",
doi = "10.1016/j.polymdegradstab.2019.02.012"
}

Mandić, M., Spasić, J., Ponjavić, M., Nikolić, M. S., Cosović, V. R., O'Connor, K., Nikodinović-Runić, J., Đokić, L.,& Jeremić, S.. (2019). Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability
Elsevier Sci Ltd, Oxford., 162, 160-168.
https://doi.org/10.1016/j.polymdegradstab.2019.02.012

Mandić M, Spasić J, Ponjavić M, Nikolić MS, Cosović VR, O'Connor K, Nikodinović-Runić J, Đokić L, Jeremić S. Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability. 2019;162:160-168.
doi:10.1016/j.polymdegradstab.2019.02.012 .

Mandić, Mina, Spasić, Jelena, Ponjavić, Marijana, Nikolić, Marija S., Cosović, Vladan R., O'Connor, Kevin, Nikodinović-Runić, Jasmina, Đokić, Lidija, Jeremić, Sanja, "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil" in Polymer Degradation and Stability, 162 (2019):160-168,
https://doi.org/10.1016/j.polymdegradstab.2019.02.012 . .

TY  - JOUR
AU  - Mandić, Mina
AU  - Spasić, Jelena
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija S.
AU  - Cosović, Vladan R.
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
AU  - Jeremić, Sanja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1246
AB  - Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Degradation and Stability
T1  - Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil
EP  - 168
SP  - 160
VL  - 162
DO  - 10.1016/j.polymdegradstab.2019.02.012
ER  - 

@article{
author = "Mandić, Mina and Spasić, Jelena and Ponjavić, Marijana and Nikolić, Marija S. and Cosović, Vladan R. and O'Connor, Kevin and Nikodinović-Runić, Jasmina and Đokić, Lidija and Jeremić, Sanja",
year = "2019",
abstract = "Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Degradation and Stability",
title = "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil",
pages = "168-160",
volume = "162",
doi = "10.1016/j.polymdegradstab.2019.02.012"
}

Mandić, M., Spasić, J., Ponjavić, M., Nikolić, M. S., Cosović, V. R., O'Connor, K., Nikodinović-Runić, J., Đokić, L.,& Jeremić, S.. (2019). Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability
Elsevier Sci Ltd, Oxford., 162, 160-168.
https://doi.org/10.1016/j.polymdegradstab.2019.02.012

Mandić M, Spasić J, Ponjavić M, Nikolić MS, Cosović VR, O'Connor K, Nikodinović-Runić J, Đokić L, Jeremić S. Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability. 2019;162:160-168.
doi:10.1016/j.polymdegradstab.2019.02.012 .

Mandić, Mina, Spasić, Jelena, Ponjavić, Marijana, Nikolić, Marija S., Cosović, Vladan R., O'Connor, Kevin, Nikodinović-Runić, Jasmina, Đokić, Lidija, Jeremić, Sanja, "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil" in Polymer Degradation and Stability, 162 (2019):160-168,
https://doi.org/10.1016/j.polymdegradstab.2019.02.012 . .

TY  - JOUR
AU  - Karkalić, Radovan
AU  - Mandić, Mina
AU  - Nikodinović-Runić, Jasmina
AU  - Jovanović, Dalibor
AU  - Luković, Zoran
AU  - Vojnović, Sandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1273
AB  - Antimicrobial and antibiofilm activity, as well as biological decontamination potential of emulsion ED-1, highly efficient in radiological decontamination of metal surfaces contaminated with uranium isotopes, was assessed. The antimicrobial potency of ED-1 was evaluated against 10 different microorganisms including four Gram-negative bacteria (Acinetobacter baumannii ATCC 19606, Escherichia coli NCTC 9001, Klebsiella pneumoniae ATCC 13803 and Pseudomonas aeruginosa NCTC 10662), four Gram-positive bacteria (Enterococcus faecalis ATCC 29212, Enterococcus faecium ATCC 6057, Listeria monocytogenes NCTC 11994, Staphylococcus aureus NCTC 6571) and two fungi (Candida albicans ATCC 10231 and Candida parapsilosis ATCC 22019). Although without strong bactericidal and fungicidal properties in standard agar diffusion assays, ED-1 effectively inhibited the growth of P. aeruginosa cells in liquid culture and more importantly, showed high potential to disperse P. aeruginosa biofilms. ED-1 was also capable to efficiently remove Bacillus subtilis ATCC 6633 spores in quantitative and a semi-quantitative biological decontamination tests on metal surfaces. Antimicrobial and antibiofilm activity and biological decontamination efficiency of ED-1 was comparable to and better than that of calcium hypochlorite solution or commercial decontaminant BX-24. This study highlighted the possibility to use ED-1, with up to 5-fold reduced amounts of calcium hypochlorite in comparison to currently used methodology, for both biological and radiological decontamination, resulting in both environmental and financial benefits.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Antimicrobial and anti-biofilm activity and biological decontamination efficiency of ED-1 emulsion
EP  - 110
IS  - 1
SP  - 99
VL  - 84
DO  - 10.2298/JSC180716087K
ER  - 

@article{
author = "Karkalić, Radovan and Mandić, Mina and Nikodinović-Runić, Jasmina and Jovanović, Dalibor and Luković, Zoran and Vojnović, Sandra",
year = "2019",
abstract = "Antimicrobial and antibiofilm activity, as well as biological decontamination potential of emulsion ED-1, highly efficient in radiological decontamination of metal surfaces contaminated with uranium isotopes, was assessed. The antimicrobial potency of ED-1 was evaluated against 10 different microorganisms including four Gram-negative bacteria (Acinetobacter baumannii ATCC 19606, Escherichia coli NCTC 9001, Klebsiella pneumoniae ATCC 13803 and Pseudomonas aeruginosa NCTC 10662), four Gram-positive bacteria (Enterococcus faecalis ATCC 29212, Enterococcus faecium ATCC 6057, Listeria monocytogenes NCTC 11994, Staphylococcus aureus NCTC 6571) and two fungi (Candida albicans ATCC 10231 and Candida parapsilosis ATCC 22019). Although without strong bactericidal and fungicidal properties in standard agar diffusion assays, ED-1 effectively inhibited the growth of P. aeruginosa cells in liquid culture and more importantly, showed high potential to disperse P. aeruginosa biofilms. ED-1 was also capable to efficiently remove Bacillus subtilis ATCC 6633 spores in quantitative and a semi-quantitative biological decontamination tests on metal surfaces. Antimicrobial and antibiofilm activity and biological decontamination efficiency of ED-1 was comparable to and better than that of calcium hypochlorite solution or commercial decontaminant BX-24. This study highlighted the possibility to use ED-1, with up to 5-fold reduced amounts of calcium hypochlorite in comparison to currently used methodology, for both biological and radiological decontamination, resulting in both environmental and financial benefits.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Antimicrobial and anti-biofilm activity and biological decontamination efficiency of ED-1 emulsion",
pages = "110-99",
number = "1",
volume = "84",
doi = "10.2298/JSC180716087K"
}

Karkalić, R., Mandić, M., Nikodinović-Runić, J., Jovanović, D., Luković, Z.,& Vojnović, S.. (2019). Antimicrobial and anti-biofilm activity and biological decontamination efficiency of ED-1 emulsion. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(1), 99-110.
https://doi.org/10.2298/JSC180716087K

Karkalić R, Mandić M, Nikodinović-Runić J, Jovanović D, Luković Z, Vojnović S. Antimicrobial and anti-biofilm activity and biological decontamination efficiency of ED-1 emulsion. in Journal of the Serbian Chemical Society. 2019;84(1):99-110.
doi:10.2298/JSC180716087K .

Karkalić, Radovan, Mandić, Mina, Nikodinović-Runić, Jasmina, Jovanović, Dalibor, Luković, Zoran, Vojnović, Sandra, "Antimicrobial and anti-biofilm activity and biological decontamination efficiency of ED-1 emulsion" in Journal of the Serbian Chemical Society, 84, no. 1 (2019):99-110,
https://doi.org/10.2298/JSC180716087K . .

TY  - JOUR
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Radivojević, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1134
AB  - Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi
EP  - 833
IS  - 6
SP  - 822
VL  - 65
DO  - 10.1002/bab.1664
ER  - 

@article{
author = "Spasić, Jelena and Mandić, Mina and Radivojević, Jelena and Jeremić, Sanja and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Đokić, Lidija",
year = "2018",
abstract = "Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi",
pages = "833-822",
number = "6",
volume = "65",
doi = "10.1002/bab.1664"
}

Spasić, J., Mandić, M., Radivojević, J., Jeremić, S., Vasiljević, B., Nikodinović-Runić, J.,& Đokić, L.. (2018). Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 65(6), 822-833.
https://doi.org/10.1002/bab.1664

Spasić J, Mandić M, Radivojević J, Jeremić S, Vasiljević B, Nikodinović-Runić J, Đokić L. Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry. 2018;65(6):822-833.
doi:10.1002/bab.1664 .

Spasić, Jelena, Mandić, Mina, Radivojević, Jelena, Jeremić, Sanja, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Đokić, Lidija, "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):822-833,
https://doi.org/10.1002/bab.1664 . .

TY  - JOUR
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Đokić, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1184
AB  - About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Streptomyces spp. in the biocatalysis toolbox
EP  - 3536
IS  - 8
SP  - 3513
VL  - 102
DO  - 10.1007/s00253-018-8884-x
ER  - 

@article{
author = "Spasić, Jelena and Mandić, Mina and Đokić, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Streptomyces spp. in the biocatalysis toolbox",
pages = "3536-3513",
number = "8",
volume = "102",
doi = "10.1007/s00253-018-8884-x"
}

Spasić, J., Mandić, M., Đokić, L.,& Nikodinović-Runić, J.. (2018). Streptomyces spp. in the biocatalysis toolbox. in Applied Microbiology and Biotechnology
Springer, New York., 102(8), 3513-3536.
https://doi.org/10.1007/s00253-018-8884-x

Spasić J, Mandić M, Đokić L, Nikodinović-Runić J. Streptomyces spp. in the biocatalysis toolbox. in Applied Microbiology and Biotechnology. 2018;102(8):3513-3536.
doi:10.1007/s00253-018-8884-x .

Spasić, Jelena, Mandić, Mina, Đokić, Lidija, Nikodinović-Runić, Jasmina, "Streptomyces spp. in the biocatalysis toolbox" in Applied Microbiology and Biotechnology, 102, no. 8 (2018):3513-3536,
https://doi.org/10.1007/s00253-018-8884-x . .

TY  - JOUR
AU  - Kanelli, Maria
AU  - Mandić, Mina
AU  - Kalakona, Margarita
AU  - Vasilakos, Sozon
AU  - Kekos, Dimitris
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1160
AB  - In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25 degrees C, pH 7.0, while the addition of ampicillin of 0.2 mg mL(-1) in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (Delta E) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties
VL  - 9
DO  - 10.3389/fmicb.2018.01495
ER  - 

@article{
author = "Kanelli, Maria and Mandić, Mina and Kalakona, Margarita and Vasilakos, Sozon and Kekos, Dimitris and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2018",
abstract = "In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25 degrees C, pH 7.0, while the addition of ampicillin of 0.2 mg mL(-1) in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (Delta E) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties",
volume = "9",
doi = "10.3389/fmicb.2018.01495"
}

Kanelli, M., Mandić, M., Kalakona, M., Vasilakos, S., Kekos, D., Nikodinović-Runić, J.,& Topakas, E.. (2018). Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.01495

Kanelli M, Mandić M, Kalakona M, Vasilakos S, Kekos D, Nikodinović-Runić J, Topakas E. Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.01495 .

Kanelli, Maria, Mandić, Mina, Kalakona, Margarita, Vasilakos, Sozon, Kekos, Dimitris, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.01495 . .

TY  - JOUR
AU  - Minovska, Gordana
AU  - Narančić, Tanja
AU  - Mandić, Mina
AU  - Šenerović, Lidija
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/691
AB  - Identifikacija i karakterizacija novih gena koji pripadaju putevima mikrobiološke razgradnje aromatičnih jedinjenja je od velikog značaja, jer su se pokazali kao izuzetno dobri biokatalizatori. U ovoj studiji, korišćenjem PCR metodologije, analizirano je prisustvo pet različitih gena iz biodegradativnog puta aromatičnih jedinjenja među 19 sredinskih izolata sa sposobnošću razgradnje širokog spektra aromatičnih jedinjenja. U slučaju 4-oksalokrotonat tautomeraze i toluen dioksigenaze, koji su detektovani kod većine sredinskih izolata, sekvence fragmenata su ukazivale na veoma ograničen diverzitet ova dva gena i visoku homologiju sa već poznatim sekvencama opisanim kod vrsta roda Pseudomonas. Korišćenjem degenerisanih prajmera konstruisanih na osnovu poznatih katehol-i naftalendioksigenaznih gena vrlo mali broj fragmenata je amplifikovan kod sredinskih izolata. Samo dve katehol 2,3-dioksigenaze iz dva izolata roda Bacillus su sekvenciranjem ukazale na različitost u odnosu na poznate sekvence, a pokazale međusobnu sličnost od 80-90%. Potencijalno tri nove katehol 1,2-dioksigenaze su identifikovane kod Bacillus sp. TN102, Gordonia sp. TN103 i Rhodococcus sp. TN112. Visok stepen homologije tautomeraza i toluen dioksigenaza među sredinskim izolatima izolovanim iz zagađene sredine ukazuje na horizontalni transfer gena, dok je ograničen uspeh u detektovanju preostala tri gena ukazao na potencijal da se među ovim izolatima mogu naći nove varijante gena iz puteva razgradnje aromatičnih jedinjenja.
AB  - Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterized corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Ograničen diverzitet gena iz puteva razgradnje aromatičnih jedinjenja među zemljišnim bakterijskim izolatima sa sposobnošću razgradnje aromatičnih jedinjenja
T1  - Limited aromatic pathway genes diversity amongst aromatic compound degrading soil bacterial isolates
EP  - 716
IS  - 3
SP  - 703
VL  - 45
DO  - 10.2298/GENSR1303703M
ER  - 

@article{
author = "Minovska, Gordana and Narančić, Tanja and Mandić, Mina and Šenerović, Lidija and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "Identifikacija i karakterizacija novih gena koji pripadaju putevima mikrobiološke razgradnje aromatičnih jedinjenja je od velikog značaja, jer su se pokazali kao izuzetno dobri biokatalizatori. U ovoj studiji, korišćenjem PCR metodologije, analizirano je prisustvo pet različitih gena iz biodegradativnog puta aromatičnih jedinjenja među 19 sredinskih izolata sa sposobnošću razgradnje širokog spektra aromatičnih jedinjenja. U slučaju 4-oksalokrotonat tautomeraze i toluen dioksigenaze, koji su detektovani kod većine sredinskih izolata, sekvence fragmenata su ukazivale na veoma ograničen diverzitet ova dva gena i visoku homologiju sa već poznatim sekvencama opisanim kod vrsta roda Pseudomonas. Korišćenjem degenerisanih prajmera konstruisanih na osnovu poznatih katehol-i naftalendioksigenaznih gena vrlo mali broj fragmenata je amplifikovan kod sredinskih izolata. Samo dve katehol 2,3-dioksigenaze iz dva izolata roda Bacillus su sekvenciranjem ukazale na različitost u odnosu na poznate sekvence, a pokazale međusobnu sličnost od 80-90%. Potencijalno tri nove katehol 1,2-dioksigenaze su identifikovane kod Bacillus sp. TN102, Gordonia sp. TN103 i Rhodococcus sp. TN112. Visok stepen homologije tautomeraza i toluen dioksigenaza među sredinskim izolatima izolovanim iz zagađene sredine ukazuje na horizontalni transfer gena, dok je ograničen uspeh u detektovanju preostala tri gena ukazao na potencijal da se među ovim izolatima mogu naći nove varijante gena iz puteva razgradnje aromatičnih jedinjenja., Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterized corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Ograničen diverzitet gena iz puteva razgradnje aromatičnih jedinjenja među zemljišnim bakterijskim izolatima sa sposobnošću razgradnje aromatičnih jedinjenja, Limited aromatic pathway genes diversity amongst aromatic compound degrading soil bacterial isolates",
pages = "716-703",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303703M"
}

Minovska, G., Narančić, T., Mandić, M., Šenerović, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Ograničen diverzitet gena iz puteva razgradnje aromatičnih jedinjenja među zemljišnim bakterijskim izolatima sa sposobnošću razgradnje aromatičnih jedinjenja. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(3), 703-716.
https://doi.org/10.2298/GENSR1303703M

Minovska G, Narančić T, Mandić M, Šenerović L, Vasiljević B, Nikodinović-Runić J. Ograničen diverzitet gena iz puteva razgradnje aromatičnih jedinjenja među zemljišnim bakterijskim izolatima sa sposobnošću razgradnje aromatičnih jedinjenja. in Genetika-Belgrade. 2013;45(3):703-716.
doi:10.2298/GENSR1303703M .

Minovska, Gordana, Narančić, Tanja, Mandić, Mina, Šenerović, Lidija, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Ograničen diverzitet gena iz puteva razgradnje aromatičnih jedinjenja među zemljišnim bakterijskim izolatima sa sposobnošću razgradnje aromatičnih jedinjenja" in Genetika-Belgrade, 45, no. 3 (2013):703-716,
https://doi.org/10.2298/GENSR1303703M . .