TY  - JOUR
AU  - Hajiboland, Roghieh
AU  - Sadeghzadeh, Noushin
AU  - Bosnić, Dragana
AU  - Bosnić, Predrag
AU  - Tolra, Roser
AU  - Poschenrieder, Charlotte
AU  - Nikolić, Miroslav
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1331
AB  - Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Selenium activates components of iron acquisition machinery in oilseed rape roots
EP  - 586
IS  - 1-2
SP  - 569
VL  - 452
DO  - 10.1007/s11104-020-04599-w
ER  - 

@article{
author = "Hajiboland, Roghieh and Sadeghzadeh, Noushin and Bosnić, Dragana and Bosnić, Predrag and Tolra, Roser and Poschenrieder, Charlotte and Nikolić, Miroslav",
year = "2020",
abstract = "Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Selenium activates components of iron acquisition machinery in oilseed rape roots",
pages = "586-569",
number = "1-2",
volume = "452",
doi = "10.1007/s11104-020-04599-w"
}

Hajiboland, R., Sadeghzadeh, N., Bosnić, D., Bosnić, P., Tolra, R., Poschenrieder, C.,& Nikolić, M.. (2020). Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil
Springer, Dordrecht., 452(1-2), 569-586.
https://doi.org/10.1007/s11104-020-04599-w

Hajiboland R, Sadeghzadeh N, Bosnić D, Bosnić P, Tolra R, Poschenrieder C, Nikolić M. Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil. 2020;452(1-2):569-586.
doi:10.1007/s11104-020-04599-w .

Hajiboland, Roghieh, Sadeghzadeh, Noushin, Bosnić, Dragana, Bosnić, Predrag, Tolra, Roser, Poschenrieder, Charlotte, Nikolić, Miroslav, "Selenium activates components of iron acquisition machinery in oilseed rape roots" in Plant and Soil, 452, no. 1-2 (2020):569-586,
https://doi.org/10.1007/s11104-020-04599-w . .

TY  - JOUR
AU  - Bosnić, Dragana
AU  - Bosnić, Predrag
AU  - Nikolić, Dragana
AU  - Nikolić, Miroslav
AU  - Samardžić, Jelena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1214
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnić, Dragana and Bosnić, Predrag and Nikolić, Dragana and Nikolić, Miroslav and Samardžić, Jelena",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnić, D., Bosnić, P., Nikolić, D., Nikolić, M.,& Samardžić, J.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnić D, Bosnić P, Nikolić D, Nikolić M, Samardžić J. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnić, Dragana, Bosnić, Predrag, Nikolić, Dragana, Nikolić, Miroslav, Samardžić, Jelena, "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Bosnić, Dragana
AU  - Jasnić, Jovana
AU  - Nikolić, Miroslav
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1154
AB  - Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Silicon mediates sodium transport and partitioning in maize under moderate salt stress
EP  - 687
SP  - 681
VL  - 155
DO  - 10.1016/j.envexpbot.2018.08.018
ER  - 

@article{
author = "Bosnić, Predrag and Bosnić, Dragana and Jasnić, Jovana and Nikolić, Miroslav",
year = "2018",
abstract = "Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Silicon mediates sodium transport and partitioning in maize under moderate salt stress",
pages = "687-681",
volume = "155",
doi = "10.1016/j.envexpbot.2018.08.018"
}

Bosnić, P., Bosnić, D., Jasnić, J.,& Nikolić, M.. (2018). Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 155, 681-687.
https://doi.org/10.1016/j.envexpbot.2018.08.018

Bosnić P, Bosnić D, Jasnić J, Nikolić M. Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany. 2018;155:681-687.
doi:10.1016/j.envexpbot.2018.08.018 .

Bosnić, Predrag, Bosnić, Dragana, Jasnić, Jovana, Nikolić, Miroslav, "Silicon mediates sodium transport and partitioning in maize under moderate salt stress" in Environmental and Experimental Botany, 155 (2018):681-687,
https://doi.org/10.1016/j.envexpbot.2018.08.018 . .