TY  - JOUR
AU  - Cumbo, Marija
AU  - Dunjić-Manevski, Sofija
AU  - Gvozdenov, Maja
AU  - Mitić, Martina Mia
AU  - Đorđević, Valentina
AU  - Tomić, Branko
PY  - 2024
UR  - https://doiserbia.nb.rs/Article.aspx?ID=0354-46642400007C
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2358
AB  - Thrombotic disorders are some of the main comorbidities in cancer patients. So far, research has indicated that thrombin, a key regulator of hemostasis, contributes to cancer progression. However, data on its origin in tumor microenvironments remain elusive. Based on previous research, we analyzed the RNA and protein expression of prothrombin, a precursor of thrombin, in selected colorectal cancer (CRC) cell lines. Since the effect of prothrombin in cancer development has not been previously reported, we treated the cells for 24 h and 48 h with different prothrombin concentrations and assessed the effect on cell proliferation and migration. Our results show that the tested CRC cell lines expressed prothrombin and that prothrombin inhibited proliferation and migration. The presented results suggest that prothrombin may contribute to CRC etiopathology and could serve as a potential diagnostic biomarker and therapeutic target. The mechanisms underlying prothrombin expression in cancer cells, potential prothrombin activation, and the underlying processes driving the described effects warrant further investigation.
PB  - Serbian Biological Society, Institute for Biological Research "Siniša Stanković"
T2  - Archives of Biological Sciences
T2  - Archives of Biological Sciences
T1  - The effect of prothrombin, the precursor of thrombin, on the proliferation and migration of colorectal cancer cells
EP  - 120
IS  - 1
SP  - 111
VL  - 76
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2358
ER  - 

@article{
author = "Cumbo, Marija and Dunjić-Manevski, Sofija and Gvozdenov, Maja and Mitić, Martina Mia and Đorđević, Valentina and Tomić, Branko",
year = "2024",
abstract = "Thrombotic disorders are some of the main comorbidities in cancer patients. So far, research has indicated that thrombin, a key regulator of hemostasis, contributes to cancer progression. However, data on its origin in tumor microenvironments remain elusive. Based on previous research, we analyzed the RNA and protein expression of prothrombin, a precursor of thrombin, in selected colorectal cancer (CRC) cell lines. Since the effect of prothrombin in cancer development has not been previously reported, we treated the cells for 24 h and 48 h with different prothrombin concentrations and assessed the effect on cell proliferation and migration. Our results show that the tested CRC cell lines expressed prothrombin and that prothrombin inhibited proliferation and migration. The presented results suggest that prothrombin may contribute to CRC etiopathology and could serve as a potential diagnostic biomarker and therapeutic target. The mechanisms underlying prothrombin expression in cancer cells, potential prothrombin activation, and the underlying processes driving the described effects warrant further investigation.",
publisher = "Serbian Biological Society, Institute for Biological Research "Siniša Stanković"",
journal = "Archives of Biological Sciences, Archives of Biological Sciences",
title = "The effect of prothrombin, the precursor of thrombin, on the proliferation and migration of colorectal cancer cells",
pages = "120-111",
number = "1",
volume = "76",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2358"
}

Cumbo, M., Dunjić-Manevski, S., Gvozdenov, M., Mitić, M. M., Đorđević, V.,& Tomić, B.. (2024). The effect of prothrombin, the precursor of thrombin, on the proliferation and migration of colorectal cancer cells. in Archives of Biological Sciences
Serbian Biological Society, Institute for Biological Research "Siniša Stanković"., 76(1), 111-120.
https://hdl.handle.net/21.15107/rcub_imagine_2358

Cumbo M, Dunjić-Manevski S, Gvozdenov M, Mitić MM, Đorđević V, Tomić B. The effect of prothrombin, the precursor of thrombin, on the proliferation and migration of colorectal cancer cells. in Archives of Biological Sciences. 2024;76(1):111-120.
https://hdl.handle.net/21.15107/rcub_imagine_2358 .

Cumbo, Marija, Dunjić-Manevski, Sofija, Gvozdenov, Maja, Mitić, Martina Mia, Đorđević, Valentina, Tomić, Branko, "The effect of prothrombin, the precursor of thrombin, on the proliferation and migration of colorectal cancer cells" in Archives of Biological Sciences, 76, no. 1 (2024):111-120,
https://hdl.handle.net/21.15107/rcub_imagine_2358 .

TY  - CONF
AU  - Divac Rankov, Aleksandra
AU  - Ušjak, Dušan
AU  - Mitić, Martina Mia
AU  - Kusić Tisma, Jelena
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1985
AB  - Cystic fibrosis (CF) is a monogenic autosomal recessive disease caused by mutations in
transmembrane conductance regulator (CFTR) gene. The golden standard for the diagnosis
of CF is sweat chloride testing (>60 mmol/L) together with the identification of two CFcausing
variants of CFTR gene. Nevertheless, about 0.01% of patients with elevated sweat
chloride and high clinical suspicion of CF do not carry any CF-causing variants.
Here we present analysis of whole exome sequencing (WES) results for two patients with
elevated sweat chloride levels and clinical presentation of CF in whom no CF-causing
mutations were detected after CFTR gene whole coding region sequencing, and large
insertion/deletion testing.
Genomic DNA was extracted from whole blood, subjected to library preparation using
DNA nanoball technology from BGI and sequenced on DNBSEQ-G400 (MGI). Produced
fastq files were mapped to hg38 reference genome using BWA/SAM tools. VCF files were
generated using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar
and AnnoVar tools. Filtering of detected variants for disease relevance was done using
the following criteria: QC Filter, GnomAD Allele Frequency, Functional consequences and
phenotype-genotype relationship.
In both patients, similar number of variants predicted to impair protein function were
detected (27 and 25). In two genes (CACNA1H and MUC5B) missense type variants
were found in both patients and loss of function variants were found in 7 and 11 genes,
respectively. Functional assessment of selected variants is underway.
Bioinformatics analyses are a valuable tool enabling identification of underlining genetic
bases of disease phenotype, important in the context of optimal patient management and
targeted therapies.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Decoding Cystic Fibrosis Phenotype
EP  - 44
SP  - 44
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1985
ER  - 

@conference{
author = "Divac Rankov, Aleksandra and Ušjak, Dušan and Mitić, Martina Mia and Kusić Tisma, Jelena",
year = "2023",
abstract = "Cystic fibrosis (CF) is a monogenic autosomal recessive disease caused by mutations in
transmembrane conductance regulator (CFTR) gene. The golden standard for the diagnosis
of CF is sweat chloride testing (>60 mmol/L) together with the identification of two CFcausing
variants of CFTR gene. Nevertheless, about 0.01% of patients with elevated sweat
chloride and high clinical suspicion of CF do not carry any CF-causing variants.
Here we present analysis of whole exome sequencing (WES) results for two patients with
elevated sweat chloride levels and clinical presentation of CF in whom no CF-causing
mutations were detected after CFTR gene whole coding region sequencing, and large
insertion/deletion testing.
Genomic DNA was extracted from whole blood, subjected to library preparation using
DNA nanoball technology from BGI and sequenced on DNBSEQ-G400 (MGI). Produced
fastq files were mapped to hg38 reference genome using BWA/SAM tools. VCF files were
generated using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar
and AnnoVar tools. Filtering of detected variants for disease relevance was done using
the following criteria: QC Filter, GnomAD Allele Frequency, Functional consequences and
phenotype-genotype relationship.
In both patients, similar number of variants predicted to impair protein function were
detected (27 and 25). In two genes (CACNA1H and MUC5B) missense type variants
were found in both patients and loss of function variants were found in 7 and 11 genes,
respectively. Functional assessment of selected variants is underway.
Bioinformatics analyses are a valuable tool enabling identification of underlining genetic
bases of disease phenotype, important in the context of optimal patient management and
targeted therapies.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Decoding Cystic Fibrosis Phenotype",
pages = "44-44",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1985"
}

Divac Rankov, A., Ušjak, D., Mitić, M. M.,& Kusić Tisma, J.. (2023). Decoding Cystic Fibrosis Phenotype. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 44-44.
https://hdl.handle.net/21.15107/rcub_imagine_1985

Divac Rankov A, Ušjak D, Mitić MM, Kusić Tisma J. Decoding Cystic Fibrosis Phenotype. in 4th Belgrade Bioinformatics Conference. 2023;4:44-44.
https://hdl.handle.net/21.15107/rcub_imagine_1985 .

Divac Rankov, Aleksandra, Ušjak, Dušan, Mitić, Martina Mia, Kusić Tisma, Jelena, "Decoding Cystic Fibrosis Phenotype" in 4th Belgrade Bioinformatics Conference, 4 (2023):44-44,
https://hdl.handle.net/21.15107/rcub_imagine_1985 .

TY  - CONF
AU  - Mitić, Martina Mia
AU  - Ušjak, Dušan
AU  - Milošević, Maja
AU  - Cumbo, Marija
AU  - Dunjić Manevski, Sofija
AU  - Tomić, Branko
AU  - Petrović, Ivana
AU  - Otašević, Petar
AU  - Micović, Slobodan
AU  - Bojić, Milovan
AU  - Đorđević, Valentina
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1999
AB  - Normal aortic valve consists of three cusps that develop in the embryonic stage. Unicuspid
aortic valve (UAV) is a rare congenital anomaly resulting in only one cusp with estimated
prevalence of 0.02% in general population. Aim of this study was to identify genetic
variants possibly associated with development of UAV. The study included 17 subjects,
namely 5 UAV patients and their healthy family members without UAV disorder. Total
DNA was isolated from venous blood samples and whole exomes sequencing (WES) was
performed using BGI’s WES protocol. Adapter-trimmed and quality-filtered reads (fastp)
were mapped to hg38 reference genome using BWA/SAMtools. VCF files were generated
using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar and AnnoVar
tools. Rare heterozygous variants present in UAV patients were found in NOTCH1, TGFB2,
MYH6, EGFR, FBN2, C1R, ROBO4 and TBX5, genes associated with development of aortic
valves. Among these, most were missense mutations with damaging effects as predicted
using in silico tools (SIFT and/or Polyphen). Only mutation in MYH6 p.Ala1130Ser was
found in at least two different UAV patients. Also, rare homozygous missense mutation
p.Gly577Ser with high damaging potential was found in ADAMTS5 gene. Besides, highly
damaging heterozygous missense mutations were detected in gene interacting functional
partners (STRING) of genes associated with development of aortic valves: DVL1, THBS1,
NOTCH4, ADAMTS3, FBN1, NOTCH2, ADAM17, LRP5, WWTR1, C1S, ANKRD6 and TNNI1,
as well as homozygous in ACAN and KNG1. Taken together, malfunctions in ADAMTS5,
ACTA2, MYH6, FBN2, AXIN1, CELSR1 or TBX5 networks were found to be common in at
least two UAV patients, suggesting existence of genetic basis in UAV disorder, possibly as
a result of combined effects of multiple variants.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Using whole exome sequencing to explore genetic basis of unicuspid aortic valve disease
EP  - 59
SP  - 59
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1999
ER  - 

@conference{
author = "Mitić, Martina Mia and Ušjak, Dušan and Milošević, Maja and Cumbo, Marija and Dunjić Manevski, Sofija and Tomić, Branko and Petrović, Ivana and Otašević, Petar and Micović, Slobodan and Bojić, Milovan and Đorđević, Valentina",
year = "2023",
abstract = "Normal aortic valve consists of three cusps that develop in the embryonic stage. Unicuspid
aortic valve (UAV) is a rare congenital anomaly resulting in only one cusp with estimated
prevalence of 0.02% in general population. Aim of this study was to identify genetic
variants possibly associated with development of UAV. The study included 17 subjects,
namely 5 UAV patients and their healthy family members without UAV disorder. Total
DNA was isolated from venous blood samples and whole exomes sequencing (WES) was
performed using BGI’s WES protocol. Adapter-trimmed and quality-filtered reads (fastp)
were mapped to hg38 reference genome using BWA/SAMtools. VCF files were generated
using GATK (BaseRecalibrator, HaplotypeCaller) and annotated with InterVar and AnnoVar
tools. Rare heterozygous variants present in UAV patients were found in NOTCH1, TGFB2,
MYH6, EGFR, FBN2, C1R, ROBO4 and TBX5, genes associated with development of aortic
valves. Among these, most were missense mutations with damaging effects as predicted
using in silico tools (SIFT and/or Polyphen). Only mutation in MYH6 p.Ala1130Ser was
found in at least two different UAV patients. Also, rare homozygous missense mutation
p.Gly577Ser with high damaging potential was found in ADAMTS5 gene. Besides, highly
damaging heterozygous missense mutations were detected in gene interacting functional
partners (STRING) of genes associated with development of aortic valves: DVL1, THBS1,
NOTCH4, ADAMTS3, FBN1, NOTCH2, ADAM17, LRP5, WWTR1, C1S, ANKRD6 and TNNI1,
as well as homozygous in ACAN and KNG1. Taken together, malfunctions in ADAMTS5,
ACTA2, MYH6, FBN2, AXIN1, CELSR1 or TBX5 networks were found to be common in at
least two UAV patients, suggesting existence of genetic basis in UAV disorder, possibly as
a result of combined effects of multiple variants.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Using whole exome sequencing to explore genetic basis of unicuspid aortic valve disease",
pages = "59-59",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1999"
}

Mitić, M. M., Ušjak, D., Milošević, M., Cumbo, M., Dunjić Manevski, S., Tomić, B., Petrović, I., Otašević, P., Micović, S., Bojić, M.,& Đorđević, V.. (2023). Using whole exome sequencing to explore genetic basis of unicuspid aortic valve disease. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 59-59.
https://hdl.handle.net/21.15107/rcub_imagine_1999

Mitić MM, Ušjak D, Milošević M, Cumbo M, Dunjić Manevski S, Tomić B, Petrović I, Otašević P, Micović S, Bojić M, Đorđević V. Using whole exome sequencing to explore genetic basis of unicuspid aortic valve disease. in 4th Belgrade Bioinformatics Conference. 2023;4:59-59.
https://hdl.handle.net/21.15107/rcub_imagine_1999 .

Mitić, Martina Mia, Ušjak, Dušan, Milošević, Maja, Cumbo, Marija, Dunjić Manevski, Sofija, Tomić, Branko, Petrović, Ivana, Otašević, Petar, Micović, Slobodan, Bojić, Milovan, Đorđević, Valentina, "Using whole exome sequencing to explore genetic basis of unicuspid aortic valve disease" in 4th Belgrade Bioinformatics Conference, 4 (2023):59-59,
https://hdl.handle.net/21.15107/rcub_imagine_1999 .

TY  - CONF
AU  - Kusić Tisma, Jelena
AU  - Ušjak, Dušan
AU  - Mitić, Martina Mia
AU  - Divac Rankov, Aleksandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2178
AB  - Background: Cystic fibrosis (CF) is autosomal
recessive disorder characterized by chronic
respiratory problems and poor growth. CF is caused
by defect in transmembrane conductance regulator
(CFTR) protein. CF is diagnosed by sweat chloride
analysis (>60 mmol/L) with the identification of
two CF-causing variants of CFTR gene. With a
longstanding history of CFTR gene analysis, our
laboratory identified several patients with elevated
sweat chloride and clinical manifestations of CF in
whom no CF-causing mutations were detected after
sequencing of whole coding region and testing for
large insertion/deletion of CFTR gene. In order to
elucidate genetic background of conditions that
mimic CF we performed whole exome sequencing
(WES) in two such patients.
Methods: Library preparation was done using
DNA nanoball technology. Produced fastq files
were mapped to hg38. VCF files were generated
using GATK and annotated with InterVar and AnnoVar
tools. Variants filtering for disease relevance was done using the following criteria: QC, GnomAD
Allele Frequency, Functional consequences
and phenotype-genotype relationship.
Results: CACNA1H and MUC5B genes were
found to be impaired in both patients. Similar number
of variants predicted to impair protein function
were detected (27 and 25) in each patient. Loss of
function variants were found in 7 and 11 genes,
respectively.
Conclusion: Further assessment of selected
variants will clarify their functional effect and relevance
for the patient’s clinical phenotype. WES
analysis will help identify genetic aspects of disease
and assist in optimal patient management in about
0.01% of patients with elevated sweat chloride and
high clinical suspicion of CF that do not carry any
CF-causing variants.
PB  - Macedonian Academy of Sciences and Arts
C3  - International Journal of Medical Genetics
T1  - POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE
EP  - 80
IS  - Supplement
SP  - 80
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2178
ER  - 

@conference{
author = "Kusić Tisma, Jelena and Ušjak, Dušan and Mitić, Martina Mia and Divac Rankov, Aleksandra",
year = "2023",
abstract = "Background: Cystic fibrosis (CF) is autosomal
recessive disorder characterized by chronic
respiratory problems and poor growth. CF is caused
by defect in transmembrane conductance regulator
(CFTR) protein. CF is diagnosed by sweat chloride
analysis (>60 mmol/L) with the identification of
two CF-causing variants of CFTR gene. With a
longstanding history of CFTR gene analysis, our
laboratory identified several patients with elevated
sweat chloride and clinical manifestations of CF in
whom no CF-causing mutations were detected after
sequencing of whole coding region and testing for
large insertion/deletion of CFTR gene. In order to
elucidate genetic background of conditions that
mimic CF we performed whole exome sequencing
(WES) in two such patients.
Methods: Library preparation was done using
DNA nanoball technology. Produced fastq files
were mapped to hg38. VCF files were generated
using GATK and annotated with InterVar and AnnoVar
tools. Variants filtering for disease relevance was done using the following criteria: QC, GnomAD
Allele Frequency, Functional consequences
and phenotype-genotype relationship.
Results: CACNA1H and MUC5B genes were
found to be impaired in both patients. Similar number
of variants predicted to impair protein function
were detected (27 and 25) in each patient. Loss of
function variants were found in 7 and 11 genes,
respectively.
Conclusion: Further assessment of selected
variants will clarify their functional effect and relevance
for the patient’s clinical phenotype. WES
analysis will help identify genetic aspects of disease
and assist in optimal patient management in about
0.01% of patients with elevated sweat chloride and
high clinical suspicion of CF that do not carry any
CF-causing variants.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "International Journal of Medical Genetics",
title = "POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE",
pages = "80-80",
number = "Supplement",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2178"
}

Kusić Tisma, J., Ušjak, D., Mitić, M. M.,& Divac Rankov, A.. (2023). POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE. in International Journal of Medical Genetics
Macedonian Academy of Sciences and Arts., 26(Supplement), 80-80.
https://hdl.handle.net/21.15107/rcub_imagine_2178

Kusić Tisma J, Ušjak D, Mitić MM, Divac Rankov A. POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE. in International Journal of Medical Genetics. 2023;26(Supplement):80-80.
https://hdl.handle.net/21.15107/rcub_imagine_2178 .

Kusić Tisma, Jelena, Ušjak, Dušan, Mitić, Martina Mia, Divac Rankov, Aleksandra, "POTENTIAL NEW GENES INVOLVED IN CYSTIC FIBROSIS PHENOTYPE" in International Journal of Medical Genetics, 26, no. Supplement (2023):80-80,
https://hdl.handle.net/21.15107/rcub_imagine_2178 .

TY  - CONF
AU  - Cumbo, Marija
AU  - Tomić, Branko
AU  - Dunjić Manevski, Sofija
AU  - Gvozdenov, Maja
AU  - Ušjak, Dušan
AU  - Mitić, Martina Mia
AU  - Đorđević, Valentina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2135
AB  - Introduction: Thrombin, crucial member of the coagulation cascade, can influence growth and development of different types of cancer. Prothrombin, thrombin precursor, although predominantly secreted
from the liver into the bloodstream, can also be expressed in the cancer cells. According to latest data prothrombin can bind in vitro to transmembrane receptors, which have previously been shown to be up-regulated in cancers and activate migration and invasion. Despite the significant amount of data on the
effects of thrombin in cancer progression, there are little data of prothrombin´s effect. The aim of this
study was to further examine the effects of prothrombin and thrombin in cancer cell lines.
Methods: Colon cancer cell lines (Caco2, SW480, SW620, HT29 and HCT116) were treated with prothrombin, thrombin and direct thrombin inhibitor, dabigatran, for 24h and 48h. To assess the effects of
treatment on cell viability and proliferation MTT test was used, and wound healing assay was used for cell
migration potential.
Results: Detected effects of treatment with prothrombin, thrombin and dabigatran varied between cell
lines. Trend of lower cell viability, proliferation and migration was observed in cells treated with prothrombin in comparison to untreated controls.
Conclusion: Our resultsindicate that prothrombin, although considered an inactive zymogen, can exert
an effect on colon cancer cells proliferation and migration in vitro.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Prothrombin influences proliferation and migration of colon cancer in vitro
EP  - 156
SP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2135
ER  - 

@conference{
author = "Cumbo, Marija and Tomić, Branko and Dunjić Manevski, Sofija and Gvozdenov, Maja and Ušjak, Dušan and Mitić, Martina Mia and Đorđević, Valentina",
year = "2023",
abstract = "Introduction: Thrombin, crucial member of the coagulation cascade, can influence growth and development of different types of cancer. Prothrombin, thrombin precursor, although predominantly secreted
from the liver into the bloodstream, can also be expressed in the cancer cells. According to latest data prothrombin can bind in vitro to transmembrane receptors, which have previously been shown to be up-regulated in cancers and activate migration and invasion. Despite the significant amount of data on the
effects of thrombin in cancer progression, there are little data of prothrombin´s effect. The aim of this
study was to further examine the effects of prothrombin and thrombin in cancer cell lines.
Methods: Colon cancer cell lines (Caco2, SW480, SW620, HT29 and HCT116) were treated with prothrombin, thrombin and direct thrombin inhibitor, dabigatran, for 24h and 48h. To assess the effects of
treatment on cell viability and proliferation MTT test was used, and wound healing assay was used for cell
migration potential.
Results: Detected effects of treatment with prothrombin, thrombin and dabigatran varied between cell
lines. Trend of lower cell viability, proliferation and migration was observed in cells treated with prothrombin in comparison to untreated controls.
Conclusion: Our resultsindicate that prothrombin, although considered an inactive zymogen, can exert
an effect on colon cancer cells proliferation and migration in vitro.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Prothrombin influences proliferation and migration of colon cancer in vitro",
pages = "156-156",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2135"
}

Cumbo, M., Tomić, B., Dunjić Manevski, S., Gvozdenov, M., Ušjak, D., Mitić, M. M.,& Đorđević, V.. (2023). Prothrombin influences proliferation and migration of colon cancer in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 156-156.
https://hdl.handle.net/21.15107/rcub_imagine_2135

Cumbo M, Tomić B, Dunjić Manevski S, Gvozdenov M, Ušjak D, Mitić MM, Đorđević V. Prothrombin influences proliferation and migration of colon cancer in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:156-156.
https://hdl.handle.net/21.15107/rcub_imagine_2135 .

Cumbo, Marija, Tomić, Branko, Dunjić Manevski, Sofija, Gvozdenov, Maja, Ušjak, Dušan, Mitić, Martina Mia, Đorđević, Valentina, "Prothrombin influences proliferation and migration of colon cancer in vitro" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):156-156,
https://hdl.handle.net/21.15107/rcub_imagine_2135 .