TY  - JOUR
AU  - Colić, Jelena
AU  - Pruner, Iva
AU  - Damjanov, Nemanja
AU  - Pekmezović, Tatjana
AU  - Sefik-Bukilica, Mirjana
AU  - Antović, Aleksandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1534
AB  - J Rheumatol 2022; doi: 10.3899/jrheum.210931

In the Methods section, under the subheading “Follow-up and study outcome,” the last sentence should be as follows: “All new DUs were recorded by contacting all 39 patients once every 1–3 months during follow-up.” The error does not affect the results or conclusions of the study.

This correction only applies to the April 1 First Release. The correct text appears in the print and online issues.
PB  - J Rheumatol Publ Co, Toronto
T2  - Journal of Rheumatology
T1  - Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis (vol 49, pg 598, 2022)
EP  - 440
IS  - 5
SP  - 440
VL  - 49
DO  - 10.3899/jrheum.210931.C1
ER  - 

@article{
author = "Colić, Jelena and Pruner, Iva and Damjanov, Nemanja and Pekmezović, Tatjana and Sefik-Bukilica, Mirjana and Antović, Aleksandra",
year = "2022",
abstract = "J Rheumatol 2022; doi: 10.3899/jrheum.210931

In the Methods section, under the subheading “Follow-up and study outcome,” the last sentence should be as follows: “All new DUs were recorded by contacting all 39 patients once every 1–3 months during follow-up.” The error does not affect the results or conclusions of the study.

This correction only applies to the April 1 First Release. The correct text appears in the print and online issues.",
publisher = "J Rheumatol Publ Co, Toronto",
journal = "Journal of Rheumatology",
title = "Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis (vol 49, pg 598, 2022)",
pages = "440-440",
number = "5",
volume = "49",
doi = "10.3899/jrheum.210931.C1"
}

Colić, J., Pruner, I., Damjanov, N., Pekmezović, T., Sefik-Bukilica, M.,& Antović, A.. (2022). Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis (vol 49, pg 598, 2022). in Journal of Rheumatology
J Rheumatol Publ Co, Toronto., 49(5), 440-440.
https://doi.org/10.3899/jrheum.210931.C1

Colić J, Pruner I, Damjanov N, Pekmezović T, Sefik-Bukilica M, Antović A. Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis (vol 49, pg 598, 2022). in Journal of Rheumatology. 2022;49(5):440-440.
doi:10.3899/jrheum.210931.C1 .

Colić, Jelena, Pruner, Iva, Damjanov, Nemanja, Pekmezović, Tatjana, Sefik-Bukilica, Mirjana, Antović, Aleksandra, "Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis (vol 49, pg 598, 2022)" in Journal of Rheumatology, 49, no. 5 (2022):440-440,
https://doi.org/10.3899/jrheum.210931.C1 . .

TY  - JOUR
AU  - Colić, Jelena
AU  - Pruner, Iva
AU  - Damjanov, Nemanja
AU  - Pekmezović, Tatjana
AU  - Sefik-Bukilica, Mirjana
AU  - Antović, Aleksandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1536
AB  - Objective. To assess thrombin generation, fibrin formation, and structure together with the fibrinolytic status in patients with systemic sclerosis (SSc) in relation to the occurrence of digital ulcers (DUs) during the course of disease. Methods. We studied variables of endothelial dysfunction, thrombin generation, overall hemostatic potential, and fibrin clot turbidity in plasma from 58 patients with SSc (39 with DU history and 19 DU-naive) and 46 matched healthy controls (HCs). Fibrin structure was visualized using scanning electron microscopy (SEM). Finally, 39 patients with a history of DUs were followed for 1.5 years and the predictive value of all investigated markers for new DU onset was explored. Results. Significantly enhanced endogenous thrombin potential (ETP) and prolonged clot lysis time (CLT) were found in patients with DUs compared to HCs. CLT was prolonged in patients with DUs compared to those without, showing good validity in identifying DUs with an area under the curve of 0.7 (95% CI 0.6-0.8). The levels of ETP and intercellular adhesion molecule 1 were independently associated with CLT. Over the follow-up period, 20 patients developed new DUs. CLT was prolonged (P  lt  0.001) in patients with new DU episodes, especially those with recurrent DUs. Regression analysis showed that the Raynaud phenomenon visual analog scale and CLT were predictors of new DUs (OR 1.1, 95% CI 1.0-1.1 and OR 1.2, 95% CI 1.1-1.3, respectively). SEM confirmed denser fibrin clots in patients with new DUs. Conclusion. Our results suggest that impaired fibrinolysis might have an emerging role in underlying digital vasculopathy and its progression in SSc.
PB  - Toronto : J Rheumatol Publ Co.
T2  - Journal of Rheumatology
T1  - Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis
EP  - 606
IS  - 6
SP  - 598
VL  - 49
DO  - 10.3899/jrheum.210931
ER  - 

@article{
author = "Colić, Jelena and Pruner, Iva and Damjanov, Nemanja and Pekmezović, Tatjana and Sefik-Bukilica, Mirjana and Antović, Aleksandra",
year = "2022",
abstract = "Objective. To assess thrombin generation, fibrin formation, and structure together with the fibrinolytic status in patients with systemic sclerosis (SSc) in relation to the occurrence of digital ulcers (DUs) during the course of disease. Methods. We studied variables of endothelial dysfunction, thrombin generation, overall hemostatic potential, and fibrin clot turbidity in plasma from 58 patients with SSc (39 with DU history and 19 DU-naive) and 46 matched healthy controls (HCs). Fibrin structure was visualized using scanning electron microscopy (SEM). Finally, 39 patients with a history of DUs were followed for 1.5 years and the predictive value of all investigated markers for new DU onset was explored. Results. Significantly enhanced endogenous thrombin potential (ETP) and prolonged clot lysis time (CLT) were found in patients with DUs compared to HCs. CLT was prolonged in patients with DUs compared to those without, showing good validity in identifying DUs with an area under the curve of 0.7 (95% CI 0.6-0.8). The levels of ETP and intercellular adhesion molecule 1 were independently associated with CLT. Over the follow-up period, 20 patients developed new DUs. CLT was prolonged (P  lt  0.001) in patients with new DU episodes, especially those with recurrent DUs. Regression analysis showed that the Raynaud phenomenon visual analog scale and CLT were predictors of new DUs (OR 1.1, 95% CI 1.0-1.1 and OR 1.2, 95% CI 1.1-1.3, respectively). SEM confirmed denser fibrin clots in patients with new DUs. Conclusion. Our results suggest that impaired fibrinolysis might have an emerging role in underlying digital vasculopathy and its progression in SSc.",
publisher = "Toronto : J Rheumatol Publ Co.",
journal = "Journal of Rheumatology",
title = "Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis",
pages = "606-598",
number = "6",
volume = "49",
doi = "10.3899/jrheum.210931"
}

Colić, J., Pruner, I., Damjanov, N., Pekmezović, T., Sefik-Bukilica, M.,& Antović, A.. (2022). Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis. in Journal of Rheumatology
Toronto : J Rheumatol Publ Co.., 49(6), 598-606.
https://doi.org/10.3899/jrheum.210931

Colić J, Pruner I, Damjanov N, Pekmezović T, Sefik-Bukilica M, Antović A. Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis. in Journal of Rheumatology. 2022;49(6):598-606.
doi:10.3899/jrheum.210931 .

Colić, Jelena, Pruner, Iva, Damjanov, Nemanja, Pekmezović, Tatjana, Sefik-Bukilica, Mirjana, Antović, Aleksandra, "Impaired Fibrinolysis Is Linked With Digital Vasculopathy and Onset of New Digital Ulcers in Systemic Sclerosis" in Journal of Rheumatology, 49, no. 6 (2022):598-606,
https://doi.org/10.3899/jrheum.210931 . .

TY  - JOUR
AU  - Stojanović, Aleksandra
AU  - Veselinović, Mirjana
AU  - Zong, Yanan
AU  - Jakovljević, Vladimir
AU  - Pruner, Iva
AU  - Antović, Aleksandra
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1454
AB  - This study sought to identify different subpopulations of extracellular vesicles (EVs) in plasma from female patients with established rheumatoid arthritis (RA) in relation to the activation of coagulation and fibrin formation in these patients. Forty women were included in the study, 20 patients and 20 age-matched healthy controls. The mean disease duration in patients was 13.0 (5.0-25.0) years, with medium to high disease activity despite ongoing treatment with low-dose prednisolone and methotrexate. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. The concentration of phosphatidylserine-positive (PS+) EVs; platelet (CD42a(+)), leucocyte (CD45(+)), monocyte (CD14(+)), and endothelial (CD144(+))-derived EVs; and EVs-expressing tissue factor (CD142(+)), P-selectin (CD62P(+)), and E-selectin (CD62E(+)) were determined by flow cytometry analysis. Overall hemostasis potential (OHP) was assessed to follow the hemostatic disturbances, including the parameters for overall coagulation potential (OCP) and overall fibrinolytic potential (OFP). Fibrin clot turbidity was measured together with clot lysis time, and scanning electron microscopy was performed. Increased concentrations of PS+, CD42a(+), CD142(+), CD45(+), CD14(+), and CD62P(+) EVs were found in plasma from patients with RA compared to healthy controls, and the concentrations of PS+, CD42a(+), CD14(+), and CD62P(+) EVs were positively correlated with the inflammatory parameters in RA patients. Positive correlations were also found between the levels of PS+ and CD42a(+) EVs and OCP as well as between the levels of PS+, CD42a(+), and CD62P(+)EVs and OHP. The levels of PS+, CD42a(+), CD14(+), CD62P(+), and CD62E(+) EVs were negatively correlated with OFP. Elevated levels of circulating EVs of different cell origins were found in patients with established RA, in relation to the inflammatory burden and coagulation activation in the disease.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Immunology
T1  - Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis
VL  - 12
DO  - 10.3389/fimmu.2021.718845
ER  - 

@article{
author = "Stojanović, Aleksandra and Veselinović, Mirjana and Zong, Yanan and Jakovljević, Vladimir and Pruner, Iva and Antović, Aleksandra",
year = "2021",
abstract = "This study sought to identify different subpopulations of extracellular vesicles (EVs) in plasma from female patients with established rheumatoid arthritis (RA) in relation to the activation of coagulation and fibrin formation in these patients. Forty women were included in the study, 20 patients and 20 age-matched healthy controls. The mean disease duration in patients was 13.0 (5.0-25.0) years, with medium to high disease activity despite ongoing treatment with low-dose prednisolone and methotrexate. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. The concentration of phosphatidylserine-positive (PS+) EVs; platelet (CD42a(+)), leucocyte (CD45(+)), monocyte (CD14(+)), and endothelial (CD144(+))-derived EVs; and EVs-expressing tissue factor (CD142(+)), P-selectin (CD62P(+)), and E-selectin (CD62E(+)) were determined by flow cytometry analysis. Overall hemostasis potential (OHP) was assessed to follow the hemostatic disturbances, including the parameters for overall coagulation potential (OCP) and overall fibrinolytic potential (OFP). Fibrin clot turbidity was measured together with clot lysis time, and scanning electron microscopy was performed. Increased concentrations of PS+, CD42a(+), CD142(+), CD45(+), CD14(+), and CD62P(+) EVs were found in plasma from patients with RA compared to healthy controls, and the concentrations of PS+, CD42a(+), CD14(+), and CD62P(+) EVs were positively correlated with the inflammatory parameters in RA patients. Positive correlations were also found between the levels of PS+ and CD42a(+) EVs and OCP as well as between the levels of PS+, CD42a(+), and CD62P(+)EVs and OHP. The levels of PS+, CD42a(+), CD14(+), CD62P(+), and CD62E(+) EVs were negatively correlated with OFP. Elevated levels of circulating EVs of different cell origins were found in patients with established RA, in relation to the inflammatory burden and coagulation activation in the disease.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Immunology",
title = "Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis",
volume = "12",
doi = "10.3389/fimmu.2021.718845"
}

Stojanović, A., Veselinović, M., Zong, Y., Jakovljević, V., Pruner, I.,& Antović, A.. (2021). Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis. in Frontiers in Immunology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fimmu.2021.718845

Stojanović A, Veselinović M, Zong Y, Jakovljević V, Pruner I, Antović A. Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis. in Frontiers in Immunology. 2021;12.
doi:10.3389/fimmu.2021.718845 .

Stojanović, Aleksandra, Veselinović, Mirjana, Zong, Yanan, Jakovljević, Vladimir, Pruner, Iva, Antović, Aleksandra, "Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis" in Frontiers in Immunology, 12 (2021),
https://doi.org/10.3389/fimmu.2021.718845 . .

TY  - JOUR
AU  - Pruner, Iva
AU  - Farm, Maria
AU  - Tomić, Branko
AU  - Gvozdenov, Maja
AU  - Kovač, Mirjana
AU  - Miljić, Predrag
AU  - Soutari, Nida Mahmoud Hourani
AU  - Antović, Aleksandra
AU  - Radojković, Dragica
AU  - Antović, Jovan P.
AU  - Đorđević, Valentina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1375
AB  - BACKGROUND: Thrombosis is a major global disease burden with almost 60% of cases related to underlying heredity and most cases still idiopathic. Synonymous single nucleotide polymorphisms (sSNPs) are considered silent and phenotypically neutral. Our previous study revealed a novel synonymous FII c.1824C gt  T variant as a potential risk factor for pregnancy loss, but it has not yet been associated with thrombotic diseases. METHODS: To determine the frequency of the FII c.1824C gt  T variant we have sequenced patients' DNA. Prothrombin RNA expression was measured by quantitative PCR. Functional analyses included routine hemostasis tests, western blotting and ELISA to determine prothrombin levels in plasma, and global hemostasis assays for thrombin and fibrin generation in carriers of the FII c.1824C gt  T variant. Scanning electron mi- croscopy was used to examine the structure of fibrin clots. RESULTS: Frequency of the FII c.1824C gt  T variant was significantly increased in patients with venous thromboembolism and cerebrovascular insult. Examination in vitro demonstrated increased expression of prothrombin mRNA in FII c.1824C gt  T transfected cells. Our ex vivo study of FII c.1824C gt  T carriers showed that the presence of this variant was associated with hyperprothrom-binemia, hypofibrinolysis, and formation of densely packed fibrin clots resistant to fibrinolysis. CONCLUSION: Our data indicate that FII c.1824C gt  T, although a synonymous variant, leads to the development of a prothrombotic phenotype and could represent a new prothrombotic risk factor. As a silent variant, FII c.1824C gt  T would probably be overlooked during genetic screening, and our results show that it could not be detected in routine laboratory tests.
PB  - Oxford Univ Press Inc, Cary
T2  - Clinical Chemistry
T1  - The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor
EP  - 389
IS  - 2
SP  - 379
VL  - 66
DO  - 10.1093/clinchem/hvz015
ER  - 

@article{
author = "Pruner, Iva and Farm, Maria and Tomić, Branko and Gvozdenov, Maja and Kovač, Mirjana and Miljić, Predrag and Soutari, Nida Mahmoud Hourani and Antović, Aleksandra and Radojković, Dragica and Antović, Jovan P. and Đorđević, Valentina",
year = "2020",
abstract = "BACKGROUND: Thrombosis is a major global disease burden with almost 60% of cases related to underlying heredity and most cases still idiopathic. Synonymous single nucleotide polymorphisms (sSNPs) are considered silent and phenotypically neutral. Our previous study revealed a novel synonymous FII c.1824C gt  T variant as a potential risk factor for pregnancy loss, but it has not yet been associated with thrombotic diseases. METHODS: To determine the frequency of the FII c.1824C gt  T variant we have sequenced patients' DNA. Prothrombin RNA expression was measured by quantitative PCR. Functional analyses included routine hemostasis tests, western blotting and ELISA to determine prothrombin levels in plasma, and global hemostasis assays for thrombin and fibrin generation in carriers of the FII c.1824C gt  T variant. Scanning electron mi- croscopy was used to examine the structure of fibrin clots. RESULTS: Frequency of the FII c.1824C gt  T variant was significantly increased in patients with venous thromboembolism and cerebrovascular insult. Examination in vitro demonstrated increased expression of prothrombin mRNA in FII c.1824C gt  T transfected cells. Our ex vivo study of FII c.1824C gt  T carriers showed that the presence of this variant was associated with hyperprothrom-binemia, hypofibrinolysis, and formation of densely packed fibrin clots resistant to fibrinolysis. CONCLUSION: Our data indicate that FII c.1824C gt  T, although a synonymous variant, leads to the development of a prothrombotic phenotype and could represent a new prothrombotic risk factor. As a silent variant, FII c.1824C gt  T would probably be overlooked during genetic screening, and our results show that it could not be detected in routine laboratory tests.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Clinical Chemistry",
title = "The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor",
pages = "389-379",
number = "2",
volume = "66",
doi = "10.1093/clinchem/hvz015"
}

Pruner, I., Farm, M., Tomić, B., Gvozdenov, M., Kovač, M., Miljić, P., Soutari, N. M. H., Antović, A., Radojković, D., Antović, J. P.,& Đorđević, V.. (2020). The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor. in Clinical Chemistry
Oxford Univ Press Inc, Cary., 66(2), 379-389.
https://doi.org/10.1093/clinchem/hvz015

Pruner I, Farm M, Tomić B, Gvozdenov M, Kovač M, Miljić P, Soutari NMH, Antović A, Radojković D, Antović JP, Đorđević V. The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor. in Clinical Chemistry. 2020;66(2):379-389.
doi:10.1093/clinchem/hvz015 .

Pruner, Iva, Farm, Maria, Tomić, Branko, Gvozdenov, Maja, Kovač, Mirjana, Miljić, Predrag, Soutari, Nida Mahmoud Hourani, Antović, Aleksandra, Radojković, Dragica, Antović, Jovan P., Đorđević, Valentina, "The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor" in Clinical Chemistry, 66, no. 2 (2020):379-389,
https://doi.org/10.1093/clinchem/hvz015 . .

TY  - JOUR
AU  - Zong, Yanan
AU  - Pruner, Iva
AU  - Antović, Aleksandra
AU  - Taxiarchis, Apostolos
AU  - Vila, Zara Pons
AU  - Soutari, Nida
AU  - Mobarrez, Fariborz
AU  - Chaireti, Roza
AU  - Widengren, Jerker
AU  - Piguet, Joachim
AU  - Antović, Jovan P.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1302
AB  - Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. MPs isolated from pooled normal human plasma were added to severe, moderate and mild HA plasma models (0%, 2.5%, 20% FVIII). The MPs' effect on hemostasis was evaluated by calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP) assays, while fibrin structure was imaged by standard confocal, stimulated emission depletion (STED) microscopy and scanning electron microscopy (SEM). MPs partially restored thrombin generation and fibrin formation in all HA plasma models. The procoagulant effect of MPs requires PS exposure, to a less extent of contact pathway activation, but not tissue factor exposure or in vitro stimulation of MPs. MPs partially normalized the fibrin structure, and using super-resolution STED, MPs attached to fibrin were clearly resolved. In summary, our results demonstrate that PS positive MPs could improve hemostasis in HA plasma models.
PB  - Nature Portfolio, Berlin
T2  - Scientific Reports
T1  - Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-64686-x
ER  - 

@article{
author = "Zong, Yanan and Pruner, Iva and Antović, Aleksandra and Taxiarchis, Apostolos and Vila, Zara Pons and Soutari, Nida and Mobarrez, Fariborz and Chaireti, Roza and Widengren, Jerker and Piguet, Joachim and Antović, Jovan P.",
year = "2020",
abstract = "Circulating microparticles (MPs) are procoagulant due to the surface containing phosphatidylserine (PS), which facilitates coagulation. We investigated if MPs improve hemostasis in HA plasma models. MPs isolated from pooled normal human plasma were added to severe, moderate and mild HA plasma models (0%, 2.5%, 20% FVIII). The MPs' effect on hemostasis was evaluated by calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP) assays, while fibrin structure was imaged by standard confocal, stimulated emission depletion (STED) microscopy and scanning electron microscopy (SEM). MPs partially restored thrombin generation and fibrin formation in all HA plasma models. The procoagulant effect of MPs requires PS exposure, to a less extent of contact pathway activation, but not tissue factor exposure or in vitro stimulation of MPs. MPs partially normalized the fibrin structure, and using super-resolution STED, MPs attached to fibrin were clearly resolved. In summary, our results demonstrate that PS positive MPs could improve hemostasis in HA plasma models.",
publisher = "Nature Portfolio, Berlin",
journal = "Scientific Reports",
title = "Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-64686-x"
}

Zong, Y., Pruner, I., Antović, A., Taxiarchis, A., Vila, Z. P., Soutari, N., Mobarrez, F., Chaireti, R., Widengren, J., Piguet, J.,& Antović, J. P.. (2020). Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models. in Scientific Reports
Nature Portfolio, Berlin., 10(1).
https://doi.org/10.1038/s41598-020-64686-x

Zong Y, Pruner I, Antović A, Taxiarchis A, Vila ZP, Soutari N, Mobarrez F, Chaireti R, Widengren J, Piguet J, Antović JP. Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models. in Scientific Reports. 2020;10(1).
doi:10.1038/s41598-020-64686-x .

Zong, Yanan, Pruner, Iva, Antović, Aleksandra, Taxiarchis, Apostolos, Vila, Zara Pons, Soutari, Nida, Mobarrez, Fariborz, Chaireti, Roza, Widengren, Jerker, Piguet, Joachim, Antović, Jovan P., "Phosphatidylserine positive microparticles improve hemostasis in in-vitro hemophilia A plasma models" in Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-64686-x . .

TY  - JOUR
AU  - Lalić-Cosić, Sanja
AU  - Dopsaj, Violeta
AU  - Kovač, Mirjana
AU  - Pruner, Iva
AU  - Littmann, Karin
AU  - Mandić-Marković, Vesna
AU  - Miković, Zeljko
AU  - Antović, Aleksandra
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1390
AB  - Introduction Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays-endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)-were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP-determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.
PB  - Wiley, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia
EP  - 330
IS  - 3
SP  - 322
VL  - 42
DO  - 10.1111/ijlh.13183
ER  - 

@article{
author = "Lalić-Cosić, Sanja and Dopsaj, Violeta and Kovač, Mirjana and Pruner, Iva and Littmann, Karin and Mandić-Marković, Vesna and Miković, Zeljko and Antović, Aleksandra",
year = "2020",
abstract = "Introduction Haemostatic balance shifted towards hypercoagulability in normal pregnancy is even more pronounced in pre-eclampsia (P-EC). The aim of this study was to analyse haemostatic disturbances and fibrin clot properties in women with pre-eclampsia and to investigate their association with maternal and foetal outcomes. Methods Forty-six pregnant women diagnosed with pre-eclampsia were included in the study, with blood sampling done on the morning following admission to hospital, as well as after delivery (mean duration 4.8 days). Two global haemostatic assays-endogenous thrombin potential (ETP) and assay of overall haemostatic potential (OHP)-were employed, including fibrin clot turbidity measurements and scanning electron microscopy (SEM) of representative samples. Results Three thrombin generation parameters (ETP, t_lag and peak height) and OHP were significantly increased in pre-eclampsia compared with controls, whereas overall fibrinolytic potential (OFP-determined as a parameter of the OHP assay) had significantly lower values. Clot lysis time was significantly prolonged in patients with pre-eclampsia. In the pre-eclamptic group after delivery, we observed a significant elevation in the peak height and a reduction in the time to peak and OFP compared with values before delivery. Pre-eclamptic patients with renal complications had significantly higher values for ETP, peak height and D-dimer. Turbidity measurements and SEM revealed dense fibrin structure in patients with pre-eclampsia. Conclusion Patients with pre-eclampsia have enhanced coagulation and impaired fibrinolysis before, and even after, delivery. In particular, the presence of multi-organ dysfunction, such as renal dysfunction, may be associated with increased thrombin generation in pre-eclampsia.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia",
pages = "330-322",
number = "3",
volume = "42",
doi = "10.1111/ijlh.13183"
}

Lalić-Cosić, S., Dopsaj, V., Kovač, M., Pruner, I., Littmann, K., Mandić-Marković, V., Miković, Z.,& Antović, A.. (2020). Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology
Wiley, Hoboken., 42(3), 322-330.
https://doi.org/10.1111/ijlh.13183

Lalić-Cosić S, Dopsaj V, Kovač M, Pruner I, Littmann K, Mandić-Marković V, Miković Z, Antović A. Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia. in International Journal of Laboratory Hematology. 2020;42(3):322-330.
doi:10.1111/ijlh.13183 .

Lalić-Cosić, Sanja, Dopsaj, Violeta, Kovač, Mirjana, Pruner, Iva, Littmann, Karin, Mandić-Marković, Vesna, Miković, Zeljko, Antović, Aleksandra, "Evaluation of global haemostatic assays and fibrin structure in patients with pre-eclampsia" in International Journal of Laboratory Hematology, 42, no. 3 (2020):322-330,
https://doi.org/10.1111/ijlh.13183 . .

TY  - JOUR
AU  - Petković, Anica
AU  - Al-Khalili, Faris
AU  - Antović, Aleksandra
AU  - Ammar, Majeed
AU  - Pruner, Iva
AU  - Vranić, Aleksandra
AU  - Soutari, Nida
AU  - Zdravković, Nebojša
AU  - Malmstrom, Rickard E.
AU  - Jakovljević, Vladimir
AU  - Antović, Jovan P.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1345
AB  - The study was aimed to evaluate the effects of two standard doses of rivaroxaban and dabigatran on global hemostatic assays in patients with atrial fibrillation. The study included 52 patients treated with rivaroxaban (15/20 mg), 50 on dabigatran (110/150 mg) and 20 healthy individuals. Platelet-poor plasma was used for determination of three global hemostatic assays, namely endogenous thrombin potential (ETP), calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP). Rivaroxaban and dabigatran reduced ETP (P lt 0.01) although OHP (P lt 0.05) was diminished only by dabigatran. Strong correlations were noticed between ETP parameters and the plasma concentrations of rivaroxaban (ETP, rUS0.51; c-max, rUS0.85; t-lag, rU0.83; t-max, rU0.66) as well as with plasma concentration of dabigatran (ETP, rUS0.75; c-max, rUS0.74; t-lag, rU0.73; t-max, rU0.52). Analysis of dabigatran concentrations under 50 ng/ml showed that ETP parameter has area under the concentration-time curvereceiver operating characteristic value of 0.879 (95% confidence interval 0.776-0.980). Dabigatran treatment paradoxically increased area under the concentration-time curve and peak values although rivaroxaban decreased peak values (P lt 0.01). However, significant correlation between CAT parameters and plasma concentration of both direct oral anticoagulants was not observed. We confirmed that the CAT assay is inappropriate for estimation of dabigatran effects and is not fully sensitive as regards rivaroxaban. The ETP assay can potentially be the appropriate method for estimation of global hemostatic capacity as regards both direct oral anticoagulants. The role of OHP needs to be confirmed in additional studies. ETP parameter of chromogenic assay has promising potential in exclusion of high plasma concentrations of dabigatran.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Blood Coagulation & Fibrinolysis
T1  - Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation
EP  - 252
IS  - 4
SP  - 243
VL  - 31
DO  - 10.1097/MBC.0000000000000907
ER  - 

@article{
author = "Petković, Anica and Al-Khalili, Faris and Antović, Aleksandra and Ammar, Majeed and Pruner, Iva and Vranić, Aleksandra and Soutari, Nida and Zdravković, Nebojša and Malmstrom, Rickard E. and Jakovljević, Vladimir and Antović, Jovan P.",
year = "2020",
abstract = "The study was aimed to evaluate the effects of two standard doses of rivaroxaban and dabigatran on global hemostatic assays in patients with atrial fibrillation. The study included 52 patients treated with rivaroxaban (15/20 mg), 50 on dabigatran (110/150 mg) and 20 healthy individuals. Platelet-poor plasma was used for determination of three global hemostatic assays, namely endogenous thrombin potential (ETP), calibrated automated thrombogram (CAT) and overall hemostasis potential (OHP). Rivaroxaban and dabigatran reduced ETP (P lt 0.01) although OHP (P lt 0.05) was diminished only by dabigatran. Strong correlations were noticed between ETP parameters and the plasma concentrations of rivaroxaban (ETP, rUS0.51; c-max, rUS0.85; t-lag, rU0.83; t-max, rU0.66) as well as with plasma concentration of dabigatran (ETP, rUS0.75; c-max, rUS0.74; t-lag, rU0.73; t-max, rU0.52). Analysis of dabigatran concentrations under 50 ng/ml showed that ETP parameter has area under the concentration-time curvereceiver operating characteristic value of 0.879 (95% confidence interval 0.776-0.980). Dabigatran treatment paradoxically increased area under the concentration-time curve and peak values although rivaroxaban decreased peak values (P lt 0.01). However, significant correlation between CAT parameters and plasma concentration of both direct oral anticoagulants was not observed. We confirmed that the CAT assay is inappropriate for estimation of dabigatran effects and is not fully sensitive as regards rivaroxaban. The ETP assay can potentially be the appropriate method for estimation of global hemostatic capacity as regards both direct oral anticoagulants. The role of OHP needs to be confirmed in additional studies. ETP parameter of chromogenic assay has promising potential in exclusion of high plasma concentrations of dabigatran.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Blood Coagulation & Fibrinolysis",
title = "Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation",
pages = "252-243",
number = "4",
volume = "31",
doi = "10.1097/MBC.0000000000000907"
}

Petković, A., Al-Khalili, F., Antović, A., Ammar, M., Pruner, I., Vranić, A., Soutari, N., Zdravković, N., Malmstrom, R. E., Jakovljević, V.,& Antović, J. P.. (2020). Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation. in Blood Coagulation & Fibrinolysis
Lippincott Williams & Wilkins, Philadelphia., 31(4), 243-252.
https://doi.org/10.1097/MBC.0000000000000907

Petković A, Al-Khalili F, Antović A, Ammar M, Pruner I, Vranić A, Soutari N, Zdravković N, Malmstrom RE, Jakovljević V, Antović JP. Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation. in Blood Coagulation & Fibrinolysis. 2020;31(4):243-252.
doi:10.1097/MBC.0000000000000907 .

Petković, Anica, Al-Khalili, Faris, Antović, Aleksandra, Ammar, Majeed, Pruner, Iva, Vranić, Aleksandra, Soutari, Nida, Zdravković, Nebojša, Malmstrom, Rickard E., Jakovljević, Vladimir, Antović, Jovan P., "Effects of rivaroxaban and dabigatran on global hemostasis in patients with atrial fibrillation" in Blood Coagulation & Fibrinolysis, 31, no. 4 (2020):243-252,
https://doi.org/10.1097/MBC.0000000000000907 . .

TY  - JOUR
AU  - Vranić, Aleksandra
AU  - Pruner, Iva
AU  - Veselinović, Mirjana
AU  - Soutari, Nida
AU  - Petković, Anica
AU  - Jakovljević, Vladimir
AU  - Antović, Aleksandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1230
AB  - Objectives This study was aimed to assess hemostatic disturbances in female patients with established rheumatoid arthritis (RA) in relation to menopausal status and disease activity. Method Ninety women were included in the study, 42 patients and 48 age-matched healthy controls. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. Two global hemostatic assays were employed, namely endogenous thrombin potential (ETP) and overall hemostasis potential (OHP). The parameters of the ETP assay (ETP, C-max, t-lag, t-max) and OHP assay (overall coagulation potential (OCP) and overall fibrinolytic potential (OFP)) were assessed. Moreover, the parameters of the fibrin clot (lag time, Max Abs, and slope) were measured by clot turbidity and scanning electron microscopy (SEM). Both patients and controls were divided into four subgroups according to menopause status. Results The premenopausal controls differed significantly from all other subgroups in terms of diminished levels of ETP (p = 0.02), C-max (p = 0.01), OCP (p = 0.02), OHP (p = 0.001), and Max Abs (p = 0.008), while OFP (p = 0.0001) was increased. This tendency was not seen in the premenopausal RA patients compared with the postmenopausal RA patients. SEM images showed denser clots composed of thinner fibers in samples from RA patients. The disease activity measured by DAS28 correlated with OCP and OHP (r = 0.54; p = 0.001 and r = 0.44; p = 0.003, respectively) indicating persistent hypercoagulable condition in the whole group of RA patients. Conclusions Our results point towards coagulation activation in premenopausal women with established RA. The patients were well characterized, which enabled assessment in a real-life setting.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Assessment of hemostatic disturbances in women with established rheumatoid arthritis
EP  - 3014
IS  - 11
SP  - 3005
VL  - 38
DO  - 10.1007/s10067-019-04629-8
ER  - 

@article{
author = "Vranić, Aleksandra and Pruner, Iva and Veselinović, Mirjana and Soutari, Nida and Petković, Anica and Jakovljević, Vladimir and Antović, Aleksandra",
year = "2019",
abstract = "Objectives This study was aimed to assess hemostatic disturbances in female patients with established rheumatoid arthritis (RA) in relation to menopausal status and disease activity. Method Ninety women were included in the study, 42 patients and 48 age-matched healthy controls. There were no differences between the investigated groups regarding the presence of traditional cardiovascular risk factors. Two global hemostatic assays were employed, namely endogenous thrombin potential (ETP) and overall hemostasis potential (OHP). The parameters of the ETP assay (ETP, C-max, t-lag, t-max) and OHP assay (overall coagulation potential (OCP) and overall fibrinolytic potential (OFP)) were assessed. Moreover, the parameters of the fibrin clot (lag time, Max Abs, and slope) were measured by clot turbidity and scanning electron microscopy (SEM). Both patients and controls were divided into four subgroups according to menopause status. Results The premenopausal controls differed significantly from all other subgroups in terms of diminished levels of ETP (p = 0.02), C-max (p = 0.01), OCP (p = 0.02), OHP (p = 0.001), and Max Abs (p = 0.008), while OFP (p = 0.0001) was increased. This tendency was not seen in the premenopausal RA patients compared with the postmenopausal RA patients. SEM images showed denser clots composed of thinner fibers in samples from RA patients. The disease activity measured by DAS28 correlated with OCP and OHP (r = 0.54; p = 0.001 and r = 0.44; p = 0.003, respectively) indicating persistent hypercoagulable condition in the whole group of RA patients. Conclusions Our results point towards coagulation activation in premenopausal women with established RA. The patients were well characterized, which enabled assessment in a real-life setting.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Assessment of hemostatic disturbances in women with established rheumatoid arthritis",
pages = "3014-3005",
number = "11",
volume = "38",
doi = "10.1007/s10067-019-04629-8"
}

Vranić, A., Pruner, I., Veselinović, M., Soutari, N., Petković, A., Jakovljević, V.,& Antović, A.. (2019). Assessment of hemostatic disturbances in women with established rheumatoid arthritis. in Clinical Rheumatology
Springer London Ltd, London., 38(11), 3005-3014.
https://doi.org/10.1007/s10067-019-04629-8

Vranić A, Pruner I, Veselinović M, Soutari N, Petković A, Jakovljević V, Antović A. Assessment of hemostatic disturbances in women with established rheumatoid arthritis. in Clinical Rheumatology. 2019;38(11):3005-3014.
doi:10.1007/s10067-019-04629-8 .

Vranić, Aleksandra, Pruner, Iva, Veselinović, Mirjana, Soutari, Nida, Petković, Anica, Jakovljević, Vladimir, Antović, Aleksandra, "Assessment of hemostatic disturbances in women with established rheumatoid arthritis" in Clinical Rheumatology, 38, no. 11 (2019):3005-3014,
https://doi.org/10.1007/s10067-019-04629-8 . .

TY  - CONF
AU  - Miljić, Predrag
AU  - Bodrozić, J.
AU  - Đorđević, Valentina
AU  - Antović, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/635
PB  - Wiley-Blackwell, Hoboken
C3  - Journal of Thrombosis and Haemostasis
T1  - Molecular markers of blood hipercoagulability and values of overall coagulation potential in double heterozygotes for the FV Leiden and FII G20210A mutation
EP  - 1014
SP  - 1013
VL  - 11
UR  - https://hdl.handle.net/21.15107/rcub_imagine_635
ER  - 

@conference{
author = "Miljić, Predrag and Bodrozić, J. and Đorđević, Valentina and Antović, Aleksandra",
year = "2013",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Thrombosis and Haemostasis",
title = "Molecular markers of blood hipercoagulability and values of overall coagulation potential in double heterozygotes for the FV Leiden and FII G20210A mutation",
pages = "1014-1013",
volume = "11",
url = "https://hdl.handle.net/21.15107/rcub_imagine_635"
}

Miljić, P., Bodrozić, J., Đorđević, V.,& Antović, A.. (2013). Molecular markers of blood hipercoagulability and values of overall coagulation potential in double heterozygotes for the FV Leiden and FII G20210A mutation. in Journal of Thrombosis and Haemostasis
Wiley-Blackwell, Hoboken., 11, 1013-1014.
https://hdl.handle.net/21.15107/rcub_imagine_635

Miljić P, Bodrozić J, Đorđević V, Antović A. Molecular markers of blood hipercoagulability and values of overall coagulation potential in double heterozygotes for the FV Leiden and FII G20210A mutation. in Journal of Thrombosis and Haemostasis. 2013;11:1013-1014.
https://hdl.handle.net/21.15107/rcub_imagine_635 .

Miljić, Predrag, Bodrozić, J., Đorđević, Valentina, Antović, Aleksandra, "Molecular markers of blood hipercoagulability and values of overall coagulation potential in double heterozygotes for the FV Leiden and FII G20210A mutation" in Journal of Thrombosis and Haemostasis, 11 (2013):1013-1014,
https://hdl.handle.net/21.15107/rcub_imagine_635 .