TY  - JOUR
AU  - Supić, Gordana
AU  - Zeljić, Katarina
AU  - Divac Rankov, Aleksandra
AU  - Kozomara, Ruzica
AU  - Nikolić, Aleksandra
AU  - Radojković, Dragica
AU  - Magić, Zvonko
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1162
AB  - Micro RNAs (miRNAs) have a major role in human cancerogenesis.The current study investigated the prognostic significance of miR-183 and miR-21 expression in tongue carcinoma patients. For qPCR of miR-183 and miR-21 expression, total RNA isolated from 60 fresh-frozen tissue of tongue carcinomas was converted into cDNA by TaqMan MicroRNA Reverse Transcription Kit and quantified by TaqMan MicroRNAs Expression Assays. Fold changes in the miRNAs expression, normalized to RNU6B, were determined using 2(-Delta Delta Ct) method, and dichotomized into high and low according to cut-off values derived from ROC curve analysis. miR-183 emerged as promising discriminatory biomarker of poor outcome. Tissue over-expression of miR-183, observed in 68.3% of tongue carcinomas, was associated with clinical stage (p = 0.037), tumor size (p = 0.036), and high alcohol intake (p = 0.034).The patients with miR-183 over-expression had significantly shorter overall survival (p = 0.006) and a 5.666 times higher risk of poor outcome (p = 0.005), while miR-21 over-expression carried a tendency towards poorer survival (p = 0.073). However, multivariate analysis revealed that the recurrences were independent adverse prognostic factors, while miR-183 over-expression lost its significance. Our results suggests that over-expression of miR-183 in tumor tissue could be a potential marker of clinical stage and a poor survival of tongue carcinoma patients and may be associated with high alcohol consumption. Oncogenic miRNAs, such as the investigated miR-183 and miR-21, could be novel prognostic biomarkers of tumor progression and adverse clinical outcome in oral cancer, as well as novel therapeutic targets in cancer.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients
EP  - 409
IS  - 1
SP  - 401
VL  - 22
DO  - 10.1007/s00784-017-2126-y
ER  - 

@article{
author = "Supić, Gordana and Zeljić, Katarina and Divac Rankov, Aleksandra and Kozomara, Ruzica and Nikolić, Aleksandra and Radojković, Dragica and Magić, Zvonko",
year = "2018",
abstract = "Micro RNAs (miRNAs) have a major role in human cancerogenesis.The current study investigated the prognostic significance of miR-183 and miR-21 expression in tongue carcinoma patients. For qPCR of miR-183 and miR-21 expression, total RNA isolated from 60 fresh-frozen tissue of tongue carcinomas was converted into cDNA by TaqMan MicroRNA Reverse Transcription Kit and quantified by TaqMan MicroRNAs Expression Assays. Fold changes in the miRNAs expression, normalized to RNU6B, were determined using 2(-Delta Delta Ct) method, and dichotomized into high and low according to cut-off values derived from ROC curve analysis. miR-183 emerged as promising discriminatory biomarker of poor outcome. Tissue over-expression of miR-183, observed in 68.3% of tongue carcinomas, was associated with clinical stage (p = 0.037), tumor size (p = 0.036), and high alcohol intake (p = 0.034).The patients with miR-183 over-expression had significantly shorter overall survival (p = 0.006) and a 5.666 times higher risk of poor outcome (p = 0.005), while miR-21 over-expression carried a tendency towards poorer survival (p = 0.073). However, multivariate analysis revealed that the recurrences were independent adverse prognostic factors, while miR-183 over-expression lost its significance. Our results suggests that over-expression of miR-183 in tumor tissue could be a potential marker of clinical stage and a poor survival of tongue carcinoma patients and may be associated with high alcohol consumption. Oncogenic miRNAs, such as the investigated miR-183 and miR-21, could be novel prognostic biomarkers of tumor progression and adverse clinical outcome in oral cancer, as well as novel therapeutic targets in cancer.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients",
pages = "409-401",
number = "1",
volume = "22",
doi = "10.1007/s00784-017-2126-y"
}

Supić, G., Zeljić, K., Divac Rankov, A., Kozomara, R., Nikolić, A., Radojković, D.,& Magić, Z.. (2018). miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 22(1), 401-409.
https://doi.org/10.1007/s00784-017-2126-y

Supić G, Zeljić K, Divac Rankov A, Kozomara R, Nikolić A, Radojković D, Magić Z. miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients. in Clinical Oral Investigations. 2018;22(1):401-409.
doi:10.1007/s00784-017-2126-y .

Supić, Gordana, Zeljić, Katarina, Divac Rankov, Aleksandra, Kozomara, Ruzica, Nikolić, Aleksandra, Radojković, Dragica, Magić, Zvonko, "miR-183 and miR-21 expression as biomarkers of progression and survival in tongue carcinoma patients" in Clinical Oral Investigations, 22, no. 1 (2018):401-409,
https://doi.org/10.1007/s00784-017-2126-y . .

TY  - CONF
AU  - Zeljić, Katarina
AU  - Supić, G.
AU  - Divac Rankov, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Kozomara, R.
AU  - Jović, N.
AU  - Radojković, Dragica
AU  - Magić, Z.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/966
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - High expressed miR-183 as a potential biomarker of poor survival in tongue cancer patients
EP  - S10
SP  - S10
VL  - 61
DO  - 10.1016/S0959-8049(16)61024-9
ER  - 

@conference{
author = "Zeljić, Katarina and Supić, G. and Divac Rankov, Aleksandra and Nikolić, Aleksandra and Kozomara, R. and Jović, N. and Radojković, Dragica and Magić, Z.",
year = "2016",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "High expressed miR-183 as a potential biomarker of poor survival in tongue cancer patients",
pages = "S10-S10",
volume = "61",
doi = "10.1016/S0959-8049(16)61024-9"
}

Zeljić, K., Supić, G., Divac Rankov, A., Nikolić, A., Kozomara, R., Jović, N., Radojković, D.,& Magić, Z.. (2016). High expressed miR-183 as a potential biomarker of poor survival in tongue cancer patients. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 61, S10-S10.
https://doi.org/10.1016/S0959-8049(16)61024-9

Zeljić K, Supić G, Divac Rankov A, Nikolić A, Kozomara R, Jović N, Radojković D, Magić Z. High expressed miR-183 as a potential biomarker of poor survival in tongue cancer patients. in European Journal of Cancer. 2016;61:S10-S10.
doi:10.1016/S0959-8049(16)61024-9 .

Zeljić, Katarina, Supić, G., Divac Rankov, Aleksandra, Nikolić, Aleksandra, Kozomara, R., Jović, N., Radojković, Dragica, Magić, Z., "High expressed miR-183 as a potential biomarker of poor survival in tongue cancer patients" in European Journal of Cancer, 61 (2016):S10-S10,
https://doi.org/10.1016/S0959-8049(16)61024-9 . .

TY  - JOUR
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Vasilijić, Sasa
AU  - Vucević, Dragana
AU  - Todorović, Vera
AU  - Supić, Gordana
AU  - Čolić, Miodrag
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/529
AB  - Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Stem Cells and Development
T1  - Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists
EP  - 708
IS  - 4
SP  - 695
VL  - 20
DO  - 10.1089/scd.2010.0145
ER  - 

@article{
author = "Tomić, Sergej and Đokić, Jelena and Vasilijić, Sasa and Vucević, Dragana and Todorović, Vera and Supić, Gordana and Čolić, Miodrag",
year = "2011",
abstract = "Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Stem Cells and Development",
title = "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists",
pages = "708-695",
number = "4",
volume = "20",
doi = "10.1089/scd.2010.0145"
}

Tomić, S., Đokić, J., Vasilijić, S., Vucević, D., Todorović, V., Supić, G.,& Čolić, M.. (2011). Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development
Mary Ann Liebert, Inc, New Rochelle., 20(4), 695-708.
https://doi.org/10.1089/scd.2010.0145

Tomić S, Đokić J, Vasilijić S, Vucević D, Todorović V, Supić G, Čolić M. Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development. 2011;20(4):695-708.
doi:10.1089/scd.2010.0145 .

Tomić, Sergej, Đokić, Jelena, Vasilijić, Sasa, Vucević, Dragana, Todorović, Vera, Supić, Gordana, Čolić, Miodrag, "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists" in Stem Cells and Development, 20, no. 4 (2011):695-708,
https://doi.org/10.1089/scd.2010.0145 . .