TY  - JOUR
AU  - Lukić, Jovanka
AU  - Gyalog, Gergő
AU  - Horváth, Zoltán
AU  - Szűcs, Anita Annamária
AU  - Ristović, Tijana
AU  - Terzić-Vidojević, Amarela
AU  - Sándor, Zsuzsanna J.
AU  - Ljubobratović, Uroš
PY  - 2023
UR  - https://www.mdpi.com/2076-2615/13/20/3179
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2107
AB  - This study aimed to evaluate different commercial diets (Otohime C1, Aller Futura (AF), Biomar Inicio Plus (BIP)) and one experimental feed (EF) in terms of their effectiveness as post-larval diets for indoor weaned largemouth bass, LMB (Micropterus salmoides). Key variations in the content of nutritive values were monounsaturated fatty acid (MUFA) and highly unsaturated FA (HUFA) ω3. Fish were fed with one of four tested diets from the 33rd to the 40th day post-hatch (DPH). Biometric indices, digestive enzyme-specific activities, thyroid hormone status, and mRNA expression of genes coding for skeleton, neuron, and muscle growth were analyzed. The lowest skeletal deformity rate and highest survival among the treatments were seen in BIP-fed fish. Dietary lipids, with an appropriate balance between MUFA and polyunsaturated FA (PUFA), alongside amino acid balance, were shown to be the main contributors to the growth of the skeleton and/or fish survival. On the other hand, fish growth is correlated with fish digestive capacity and feed moisture percent rather than feed quality. Unexpectedly, BIP-fed fish were attributed with the lowest expression of skeleton differentiation markers, which may reflect the sacrifice of scale and/or cranium growth at the expense of somatic growth. This study highlights the role of non-marine ingredients in the nutrition of post-larval LMB.
T2  - Animals
T2  - Animals
T1  - Evaluation of Post-Larval Diets for Indoor Weaned Largemouth Bass (Micropterus salmoides)
IS  - 20
SP  - 3179
VL  - 13
DO  - 10.3390/ani13203179
ER  - 

@article{
author = "Lukić, Jovanka and Gyalog, Gergő and Horváth, Zoltán and Szűcs, Anita Annamária and Ristović, Tijana and Terzić-Vidojević, Amarela and Sándor, Zsuzsanna J. and Ljubobratović, Uroš",
year = "2023",
abstract = "This study aimed to evaluate different commercial diets (Otohime C1, Aller Futura (AF), Biomar Inicio Plus (BIP)) and one experimental feed (EF) in terms of their effectiveness as post-larval diets for indoor weaned largemouth bass, LMB (Micropterus salmoides). Key variations in the content of nutritive values were monounsaturated fatty acid (MUFA) and highly unsaturated FA (HUFA) ω3. Fish were fed with one of four tested diets from the 33rd to the 40th day post-hatch (DPH). Biometric indices, digestive enzyme-specific activities, thyroid hormone status, and mRNA expression of genes coding for skeleton, neuron, and muscle growth were analyzed. The lowest skeletal deformity rate and highest survival among the treatments were seen in BIP-fed fish. Dietary lipids, with an appropriate balance between MUFA and polyunsaturated FA (PUFA), alongside amino acid balance, were shown to be the main contributors to the growth of the skeleton and/or fish survival. On the other hand, fish growth is correlated with fish digestive capacity and feed moisture percent rather than feed quality. Unexpectedly, BIP-fed fish were attributed with the lowest expression of skeleton differentiation markers, which may reflect the sacrifice of scale and/or cranium growth at the expense of somatic growth. This study highlights the role of non-marine ingredients in the nutrition of post-larval LMB.",
journal = "Animals, Animals",
title = "Evaluation of Post-Larval Diets for Indoor Weaned Largemouth Bass (Micropterus salmoides)",
number = "20",
pages = "3179",
volume = "13",
doi = "10.3390/ani13203179"
}

Lukić, J., Gyalog, G., Horváth, Z., Szűcs, A. A., Ristović, T., Terzić-Vidojević, A., Sándor, Z. J.,& Ljubobratović, U.. (2023). Evaluation of Post-Larval Diets for Indoor Weaned Largemouth Bass (Micropterus salmoides). in Animals, 13(20), 3179.
https://doi.org/10.3390/ani13203179

Lukić J, Gyalog G, Horváth Z, Szűcs AA, Ristović T, Terzić-Vidojević A, Sándor ZJ, Ljubobratović U. Evaluation of Post-Larval Diets for Indoor Weaned Largemouth Bass (Micropterus salmoides). in Animals. 2023;13(20):3179.
doi:10.3390/ani13203179 .

Lukić, Jovanka, Gyalog, Gergő, Horváth, Zoltán, Szűcs, Anita Annamária, Ristović, Tijana, Terzić-Vidojević, Amarela, Sándor, Zsuzsanna J., Ljubobratović, Uroš, "Evaluation of Post-Larval Diets for Indoor Weaned Largemouth Bass (Micropterus salmoides)" in Animals, 13, no. 20 (2023):3179,
https://doi.org/10.3390/ani13203179 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Fazekas, Georgina
AU  - Koljukaj, Alan
AU  - Ristović, Tijana
AU  - Vass, Vivien
AU  - Ardo, Laszlo
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Pesić, Mirjana
AU  - Milincić, Danijel
AU  - Kostić, Aleksandar
AU  - Lukić, Jovanka
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1448
AB  - This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding
VL  - 542
DO  - 10.1016/j.aquaculture.2021.736901
ER  - 

@article{
author = "Ljubobratović, Uros and Fazekas, Georgina and Koljukaj, Alan and Ristović, Tijana and Vass, Vivien and Ardo, Laszlo and Stanisavljević, Nemanja and Vukotić, Goran and Pesić, Mirjana and Milincić, Danijel and Kostić, Aleksandar and Lukić, Jovanka",
year = "2021",
abstract = "This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding",
volume = "542",
doi = "10.1016/j.aquaculture.2021.736901"
}

Ljubobratović, U., Fazekas, G., Koljukaj, A., Ristović, T., Vass, V., Ardo, L., Stanisavljević, N., Vukotić, G., Pesić, M., Milincić, D., Kostić, A.,& Lukić, J.. (2021). Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture
Elsevier, Amsterdam., 542.
https://doi.org/10.1016/j.aquaculture.2021.736901

Ljubobratović U, Fazekas G, Koljukaj A, Ristović T, Vass V, Ardo L, Stanisavljević N, Vukotić G, Pesić M, Milincić D, Kostić A, Lukić J. Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture. 2021;542.
doi:10.1016/j.aquaculture.2021.736901 .

Ljubobratović, Uros, Fazekas, Georgina, Koljukaj, Alan, Ristović, Tijana, Vass, Vivien, Ardo, Laszlo, Stanisavljević, Nemanja, Vukotić, Goran, Pesić, Mirjana, Milincić, Danijel, Kostić, Aleksandar, Lukić, Jovanka, "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding" in Aquaculture, 542 (2021),
https://doi.org/10.1016/j.aquaculture.2021.736901 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Kosanović, Dejana
AU  - Vukotić, Goran
AU  - Molnar, Zsuzsanna
AU  - Stanisavljević, Nemanja
AU  - Ristović, Tijana
AU  - Peter, Geza
AU  - Lukić, Jovanka
AU  - Jeney, Galina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1026
AB  - This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study
EP  - 462
SP  - 451
VL  - 115
DO  - 10.1016/j.rvsc.2017.07.018
ER  - 

@article{
author = "Ljubobratović, Uros and Kosanović, Dejana and Vukotić, Goran and Molnar, Zsuzsanna and Stanisavljević, Nemanja and Ristović, Tijana and Peter, Geza and Lukić, Jovanka and Jeney, Galina",
year = "2017",
abstract = "This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study",
pages = "462-451",
volume = "115",
doi = "10.1016/j.rvsc.2017.07.018"
}

Ljubobratović, U., Kosanović, D., Vukotić, G., Molnar, Z., Stanisavljević, N., Ristović, T., Peter, G., Lukić, J.,& Jeney, G.. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 115, 451-462.
https://doi.org/10.1016/j.rvsc.2017.07.018

Ljubobratović U, Kosanović D, Vukotić G, Molnar Z, Stanisavljević N, Ristović T, Peter G, Lukić J, Jeney G. Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science. 2017;115:451-462.
doi:10.1016/j.rvsc.2017.07.018 .

Ljubobratović, Uros, Kosanović, Dejana, Vukotić, Goran, Molnar, Zsuzsanna, Stanisavljević, Nemanja, Ristović, Tijana, Peter, Geza, Lukić, Jovanka, Jeney, Galina, "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study" in Research in Veterinary Science, 115 (2017):451-462,
https://doi.org/10.1016/j.rvsc.2017.07.018 . .