TY  - CHAP
AU  - Đorđević, Neda
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1822
AB  - Ova tema je fokusirana na hemijsku i biološku karakterizaciju odabranih uzoraka crvenih vina. Akcenat
je stavljen na hemijsku karakterizaciju klonskih sortnih vina vranac i merlo, kao i na procenu antioksidativne
aktivnosti merlo vina u ćelijama kvasca. U radu su primenjene brojne analitičke tehnike za
određivanje koncentracije fenolnih jedinjenja, elementalnog sastava i in vitro antioksidativnu aktivnosti
ispitivanih uzoraka (spektrofotometrija, ciklična voltametrija, klasična polarografija, EPR spektroskopija,
LC-MS/MS, ICP-OES i ICP-MS). Pored antioksidativne, ispitane su i citotoksična i antibakterijska aktivnost odgovarajućih
uzoraka vina. Eksperiment na bazi vinskog kvasca je obuhvatio određivanje ukupnih –SH
grupa, kao i enzimskih aktivnosti glutation peroksidaze, glutation reduktaze i katalaze. Eksperimentalno
dobijeni podaci za klonska sortna vina merlo ukazuju na poseban doprinos glutation peroksidaze preživljavanju
ćelija kvasca u uslovima oksidativnog stresa. Konačno, primenom veštačke neuronske mreže razvijena
je brza i precizna metoda za predviđanje uticaja malih promena u vinskom fenolnom profilu na
aktivnosti istaknutih enzima antioksidativne zaštite.
AB  - This topic focuses on the chemical and biological characterization of selected red wine samples. Emphasis
is placed on the chemical characterisation of clonal wines of Vranac and Merlot varieties, as well as
on the evaluation of the antioxidant activity of Merlot wines in yeast cells. Numerous analytical techniques
were used to determine the concentration of phenolic compounds, elemental composition and in
vitro antioxidant activity of the tested samples (spectrophotometry, cyclic voltammetry, classical polarography,
EPR spectroscopy, LC-MS/MS, ICP-OES and ICP-MS). In addition, total antioxidant activity, cytotoxic
and antibacterial activity of the respective wine samples were determined. The wine yeast experiment
involved determining the total groups –SH, as well as enzymatic activities of glutathione peroxidase, glutathione
reductase and catalase. The experimentally obtained data for clonal Merlot wine varieties indicate
a particular contribution of glutathione peroxidase to the survival of yeast cells under oxidative stress conditions.
Finally, using an artificial neural network (ANN), a rapid and accurate method was developed to predict
the effect of small changes in the wine phenolic profile on the activities of prominent antioxidant
enzymes.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Hemijski profil i antioksidativna aktivnost crvenih vina klonova autohtone i internacionalnih sorti vinove loze
T1  - Chemical profile and antioxidative activity of red wines obtained from autochthonous and international grape clone varieties
EP  - 222
IS  - 2
SP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1822
ER  - 

@inbook{
author = "Đorđević, Neda",
year = "2022",
abstract = "Ova tema je fokusirana na hemijsku i biološku karakterizaciju odabranih uzoraka crvenih vina. Akcenat
je stavljen na hemijsku karakterizaciju klonskih sortnih vina vranac i merlo, kao i na procenu antioksidativne
aktivnosti merlo vina u ćelijama kvasca. U radu su primenjene brojne analitičke tehnike za
određivanje koncentracije fenolnih jedinjenja, elementalnog sastava i in vitro antioksidativnu aktivnosti
ispitivanih uzoraka (spektrofotometrija, ciklična voltametrija, klasična polarografija, EPR spektroskopija,
LC-MS/MS, ICP-OES i ICP-MS). Pored antioksidativne, ispitane su i citotoksična i antibakterijska aktivnost odgovarajućih
uzoraka vina. Eksperiment na bazi vinskog kvasca je obuhvatio određivanje ukupnih –SH
grupa, kao i enzimskih aktivnosti glutation peroksidaze, glutation reduktaze i katalaze. Eksperimentalno
dobijeni podaci za klonska sortna vina merlo ukazuju na poseban doprinos glutation peroksidaze preživljavanju
ćelija kvasca u uslovima oksidativnog stresa. Konačno, primenom veštačke neuronske mreže razvijena
je brza i precizna metoda za predviđanje uticaja malih promena u vinskom fenolnom profilu na
aktivnosti istaknutih enzima antioksidativne zaštite., This topic focuses on the chemical and biological characterization of selected red wine samples. Emphasis
is placed on the chemical characterisation of clonal wines of Vranac and Merlot varieties, as well as
on the evaluation of the antioxidant activity of Merlot wines in yeast cells. Numerous analytical techniques
were used to determine the concentration of phenolic compounds, elemental composition and in
vitro antioxidant activity of the tested samples (spectrophotometry, cyclic voltammetry, classical polarography,
EPR spectroscopy, LC-MS/MS, ICP-OES and ICP-MS). In addition, total antioxidant activity, cytotoxic
and antibacterial activity of the respective wine samples were determined. The wine yeast experiment
involved determining the total groups –SH, as well as enzymatic activities of glutathione peroxidase, glutathione
reductase and catalase. The experimentally obtained data for clonal Merlot wine varieties indicate
a particular contribution of glutathione peroxidase to the survival of yeast cells under oxidative stress conditions.
Finally, using an artificial neural network (ANN), a rapid and accurate method was developed to predict
the effect of small changes in the wine phenolic profile on the activities of prominent antioxidant
enzymes.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Hemijski profil i antioksidativna aktivnost crvenih vina klonova autohtone i internacionalnih sorti vinove loze, Chemical profile and antioxidative activity of red wines obtained from autochthonous and international grape clone varieties",
pages = "222-206",
number = "2",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1822"
}

Đorđević, N.. (2022). Hemijski profil i antioksidativna aktivnost crvenih vina klonova autohtone i internacionalnih sorti vinove loze. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(2), 206-222.
https://hdl.handle.net/21.15107/rcub_imagine_1822

Đorđević N. Hemijski profil i antioksidativna aktivnost crvenih vina klonova autohtone i internacionalnih sorti vinove loze. in Trendovi u molekularnoj Biologiji. 2022;(2):206-222.
https://hdl.handle.net/21.15107/rcub_imagine_1822 .

Đorđević, Neda, "Hemijski profil i antioksidativna aktivnost crvenih vina klonova autohtone i internacionalnih sorti vinove loze" in Trendovi u molekularnoj Biologiji, no. 2 (2022):206-222,
https://hdl.handle.net/21.15107/rcub_imagine_1822 .

TY  - JOUR
AU  - Đorđević, Neda O.
AU  - Stanisavljević, Nemanja
AU  - Todorovic Vukotić, Nevena
AU  - Novović, Katarina
AU  - Zakula, Jelena J.
AU  - Stanković, Dalibor
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1584
AB  - The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix
DO  - 10.1080/14786419.2022.2095380
ER  - 

@article{
author = "Đorđević, Neda O. and Stanisavljević, Nemanja and Todorovic Vukotić, Nevena and Novović, Katarina and Zakula, Jelena J. and Stanković, Dalibor and Pajović, Snežana B.",
year = "2022",
abstract = "The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix",
doi = "10.1080/14786419.2022.2095380"
}

Đorđević, N. O., Stanisavljević, N., Todorovic Vukotić, N., Novović, K., Zakula, J. J., Stanković, D.,& Pajović, S. B.. (2022). Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research
Taylor & Francis Ltd, Abingdon..
https://doi.org/10.1080/14786419.2022.2095380

Đorđević NO, Stanisavljević N, Todorovic Vukotić N, Novović K, Zakula JJ, Stanković D, Pajović SB. Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research. 2022;.
doi:10.1080/14786419.2022.2095380 .

Đorđević, Neda O., Stanisavljević, Nemanja, Todorovic Vukotić, Nevena, Novović, Katarina, Zakula, Jelena J., Stanković, Dalibor, Pajović, Snežana B., "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix" in Natural Product Research (2022),
https://doi.org/10.1080/14786419.2022.2095380 . .