TY  - CONF
AU  - Curčić, Jovana
AU  - Matijasević, Danka
AU  - Stanisavljević, Nemanja
AU  - Tasić, Srđan
AU  - Kojić, Milan
AU  - Malešević, Milka
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2057
AB  - Thermal springs represent a habitat with extreme conditions that harbor a unique
microbial community adapted to thrive in this environment. In addition to the geothermal
springs in Iceland, the thermal springs of Vranjska Banja are considered the hottest in
Europe with a water temperature of 63-95°C. Due to global warming and climate change,
there is a growing need for knowledge about the biodiversity of extreme natural habitats.
Besides the exceptional importance of studying extremophilic microorganisms, the
difficulty in their cultivation limits the expanding necessity of research in this field. This
study provides information about the microbial community structure and physicochemical
characteristics of the thermal spring of Vranjska Banja. To determine and monitor the
microbiota diversity of the Vranjska Banja hot spring, for the first time, comprehensive
culture-independent metagenomic analysis in parallel with a culture-dependent
approach was applied. The culture-independent composition of bacterial communities of
the thermal water was investigated using MiSeq-Illumina technology and analyzed by
the computing environment QIIME2 v2021. The applied cultivation approach resulted
in the isolation of 17 strains belonging to genera Bacillus, Anoxybacillus, Hydrogenophilus,
and Geobacillus, based on 16S rRNA sequencing and whole genome sequencing of five
representative strains has been performed. The complete DNA was sequenced using
Illumina HiSeq from the MicrobesNG service. Genomic characterization and OrthoANI
analysis have shown that two of them are candidates for novel species. Products of
extremophilic microorganisms adapted to harsh conditions have great potential to be
used for biotechnological research and industrial application. Results of BAGEL4 and
AntiSMASH showed that the sequenced strains from Vranjska Banja hot spring have
the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase,
chitinase, and glucanase) and various antimicrobial molecules.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia
EP  - 112
SP  - 112
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2057
ER  - 

@conference{
author = "Curčić, Jovana and Matijasević, Danka and Stanisavljević, Nemanja and Tasić, Srđan and Kojić, Milan and Malešević, Milka",
year = "2023",
abstract = "Thermal springs represent a habitat with extreme conditions that harbor a unique
microbial community adapted to thrive in this environment. In addition to the geothermal
springs in Iceland, the thermal springs of Vranjska Banja are considered the hottest in
Europe with a water temperature of 63-95°C. Due to global warming and climate change,
there is a growing need for knowledge about the biodiversity of extreme natural habitats.
Besides the exceptional importance of studying extremophilic microorganisms, the
difficulty in their cultivation limits the expanding necessity of research in this field. This
study provides information about the microbial community structure and physicochemical
characteristics of the thermal spring of Vranjska Banja. To determine and monitor the
microbiota diversity of the Vranjska Banja hot spring, for the first time, comprehensive
culture-independent metagenomic analysis in parallel with a culture-dependent
approach was applied. The culture-independent composition of bacterial communities of
the thermal water was investigated using MiSeq-Illumina technology and analyzed by
the computing environment QIIME2 v2021. The applied cultivation approach resulted
in the isolation of 17 strains belonging to genera Bacillus, Anoxybacillus, Hydrogenophilus,
and Geobacillus, based on 16S rRNA sequencing and whole genome sequencing of five
representative strains has been performed. The complete DNA was sequenced using
Illumina HiSeq from the MicrobesNG service. Genomic characterization and OrthoANI
analysis have shown that two of them are candidates for novel species. Products of
extremophilic microorganisms adapted to harsh conditions have great potential to be
used for biotechnological research and industrial application. Results of BAGEL4 and
AntiSMASH showed that the sequenced strains from Vranjska Banja hot spring have
the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase,
chitinase, and glucanase) and various antimicrobial molecules.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia",
pages = "112-112",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2057"
}

Curčić, J., Matijasević, D., Stanisavljević, N., Tasić, S., Kojić, M.,& Malešević, M.. (2023). Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 112-112.
https://hdl.handle.net/21.15107/rcub_imagine_2057

Curčić J, Matijasević D, Stanisavljević N, Tasić S, Kojić M, Malešević M. Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in 4th Belgrade Bioinformatics Conference. 2023;4:112-112.
https://hdl.handle.net/21.15107/rcub_imagine_2057 .

Curčić, Jovana, Matijasević, Danka, Stanisavljević, Nemanja, Tasić, Srđan, Kojić, Milan, Malešević, Milka, "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia" in 4th Belgrade Bioinformatics Conference, 4 (2023):112-112,
https://hdl.handle.net/21.15107/rcub_imagine_2057 .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Matijašević, Danka
AU  - Ćurčić, Jovana
AU  - Tasić, Vukašin
AU  - Tasić, Srđan
AU  - Kojić, Milan
PY  - 2023
UR  - https://doi.org/10.1007/s00248-023-02242-6
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1893
AB  - The hot spring Vranjska Banja is the hottest spring on the Balkan Peninsula with a water temperature of 63–95 °C and a pH value of 7.1, in situ. According to the physicochemical analysis, Vranjska Banja hot spring belongs to the bicarbonated and sulfated hyperthermal waters. The structures of microbial community of this geothermal spring are still largely unexplored. In order to determine and monitor the diversity of microbiota of the Vranjska Banja hot spring, a comprehensive culture-independent metagenomic analysis was conducted in parallel with a culture-dependent approach for the first time. Microbial profiling using amplicon sequencing analysis revealed the presence of phylogenetically novel taxa, ranging from species to phyla. Cultivation-based methods resulted in the isolation of 17 strains belonging to the genera Anoxybacillus, Bacillus, Geobacillus, and Hydrogenophillus. Whole-genome sequencing of five representative strains was then performed. The genomic characterization and OrthoANI analysis revealed that the Vranjska Banja hot spring harbors phylogenetically novel species of the genus Anoxybacillus, proving its uniqueness. Moreover, these isolates contain stress response genes that enable them to survive in the harsh conditions of the hot springs. The results of the in silico analysis show that most of the sequenced strains have the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase, chitinase, and glucanase) and various antimicrobial molecules that can be of great importance for industrial, agricultural, and biotechnological applications. Finally, this study provides a basis for further research and understanding of the metabolic potential of these microorganisms.
T2  - Microbial Ecology
T2  - Microbial EcologyMicrob Ecol
T1  - Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia
DO  - 10.1007/s00248-023-02242-6
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Matijašević, Danka and Ćurčić, Jovana and Tasić, Vukašin and Tasić, Srđan and Kojić, Milan",
year = "2023",
abstract = "The hot spring Vranjska Banja is the hottest spring on the Balkan Peninsula with a water temperature of 63–95 °C and a pH value of 7.1, in situ. According to the physicochemical analysis, Vranjska Banja hot spring belongs to the bicarbonated and sulfated hyperthermal waters. The structures of microbial community of this geothermal spring are still largely unexplored. In order to determine and monitor the diversity of microbiota of the Vranjska Banja hot spring, a comprehensive culture-independent metagenomic analysis was conducted in parallel with a culture-dependent approach for the first time. Microbial profiling using amplicon sequencing analysis revealed the presence of phylogenetically novel taxa, ranging from species to phyla. Cultivation-based methods resulted in the isolation of 17 strains belonging to the genera Anoxybacillus, Bacillus, Geobacillus, and Hydrogenophillus. Whole-genome sequencing of five representative strains was then performed. The genomic characterization and OrthoANI analysis revealed that the Vranjska Banja hot spring harbors phylogenetically novel species of the genus Anoxybacillus, proving its uniqueness. Moreover, these isolates contain stress response genes that enable them to survive in the harsh conditions of the hot springs. The results of the in silico analysis show that most of the sequenced strains have the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase, chitinase, and glucanase) and various antimicrobial molecules that can be of great importance for industrial, agricultural, and biotechnological applications. Finally, this study provides a basis for further research and understanding of the metabolic potential of these microorganisms.",
journal = "Microbial Ecology, Microbial EcologyMicrob Ecol",
title = "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia",
doi = "10.1007/s00248-023-02242-6"
}

Malešević, M., Stanisavljević, N., Matijašević, D., Ćurčić, J., Tasić, V., Tasić, S.,& Kojić, M.. (2023). Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in Microbial Ecology.
https://doi.org/10.1007/s00248-023-02242-6

Malešević M, Stanisavljević N, Matijašević D, Ćurčić J, Tasić V, Tasić S, Kojić M. Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in Microbial Ecology. 2023;.
doi:10.1007/s00248-023-02242-6 .

Malešević, Milka, Stanisavljević, Nemanja, Matijašević, Danka, Ćurčić, Jovana, Tasić, Vukašin, Tasić, Srđan, Kojić, Milan, "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia" in Microbial Ecology (2023),
https://doi.org/10.1007/s00248-023-02242-6 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Matijašević, Danka
AU  - Ćurčić, Jovana
AU  - Tasić, Vukašin
AU  - Tasić, Srđan
AU  - Kojić, Milan
PY  - 2023
UR  - https://doi.org/10.1007/s00248-023-02242-6
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1931
AB  - The hot spring Vranjska Banja is the hottest spring on the Balkan Peninsula with a water temperature of 63–95 °C and a pH value of 7.1, in situ. According to the physicochemical analysis, Vranjska Banja hot spring belongs to the bicarbonated and sulfated hyperthermal waters. The structures of microbial community of this geothermal spring are still largely unexplored. In order to determine and monitor the diversity of microbiota of the Vranjska Banja hot spring, a comprehensive culture-independent metagenomic analysis was conducted in parallel with a culture-dependent approach for the first time. Microbial profiling using amplicon sequencing analysis revealed the presence of phylogenetically novel taxa, ranging from species to phyla. Cultivation-based methods resulted in the isolation of 17 strains belonging to the genera Anoxybacillus, Bacillus, Geobacillus, and Hydrogenophillus. Whole-genome sequencing of five representative strains was then performed. The genomic characterization and OrthoANI analysis revealed that the Vranjska Banja hot spring harbors phylogenetically novel species of the genus Anoxybacillus, proving its uniqueness. Moreover, these isolates contain stress response genes that enable them to survive in the harsh conditions of the hot springs. The results of the in silico analysis show that most of the sequenced strains have the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase, chitinase, and glucanase) and various antimicrobial molecules that can be of great importance for industrial, agricultural, and biotechnological applications. Finally, this study provides a basis for further research and understanding of the metabolic potential of these microorganisms.
T2  - Microbial Ecology
T2  - Microbial EcologyMicrob Ecol
T1  - Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia
DO  - 10.1007/s00248-023-02242-6
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Matijašević, Danka and Ćurčić, Jovana and Tasić, Vukašin and Tasić, Srđan and Kojić, Milan",
year = "2023",
abstract = "The hot spring Vranjska Banja is the hottest spring on the Balkan Peninsula with a water temperature of 63–95 °C and a pH value of 7.1, in situ. According to the physicochemical analysis, Vranjska Banja hot spring belongs to the bicarbonated and sulfated hyperthermal waters. The structures of microbial community of this geothermal spring are still largely unexplored. In order to determine and monitor the diversity of microbiota of the Vranjska Banja hot spring, a comprehensive culture-independent metagenomic analysis was conducted in parallel with a culture-dependent approach for the first time. Microbial profiling using amplicon sequencing analysis revealed the presence of phylogenetically novel taxa, ranging from species to phyla. Cultivation-based methods resulted in the isolation of 17 strains belonging to the genera Anoxybacillus, Bacillus, Geobacillus, and Hydrogenophillus. Whole-genome sequencing of five representative strains was then performed. The genomic characterization and OrthoANI analysis revealed that the Vranjska Banja hot spring harbors phylogenetically novel species of the genus Anoxybacillus, proving its uniqueness. Moreover, these isolates contain stress response genes that enable them to survive in the harsh conditions of the hot springs. The results of the in silico analysis show that most of the sequenced strains have the potential to produce thermostable enzymes (proteases, lipases, amylases, phytase, chitinase, and glucanase) and various antimicrobial molecules that can be of great importance for industrial, agricultural, and biotechnological applications. Finally, this study provides a basis for further research and understanding of the metabolic potential of these microorganisms.",
journal = "Microbial Ecology, Microbial EcologyMicrob Ecol",
title = "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia",
doi = "10.1007/s00248-023-02242-6"
}

Malešević, M., Stanisavljević, N., Matijašević, D., Ćurčić, J., Tasić, V., Tasić, S.,& Kojić, M.. (2023). Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in Microbial Ecology.
https://doi.org/10.1007/s00248-023-02242-6

Malešević M, Stanisavljević N, Matijašević D, Ćurčić J, Tasić V, Tasić S, Kojić M. Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia. in Microbial Ecology. 2023;.
doi:10.1007/s00248-023-02242-6 .

Malešević, Milka, Stanisavljević, Nemanja, Matijašević, Danka, Ćurčić, Jovana, Tasić, Vukašin, Tasić, Srđan, Kojić, Milan, "Metagenomic Analysis of Bacterial Community and Isolation of Representative Strains from Vranjska Banja Hot Spring, Serbia" in Microbial Ecology (2023),
https://doi.org/10.1007/s00248-023-02242-6 . .

TY  - JOUR
AU  - Tasić, Srđan
AU  - Obradović, Dragojlo
AU  - Kojić, Milan
AU  - Tasić, Irena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/500
AB  - Predmet biohemijske i molekularne karakterizacije bio je dominantan soj ST5 vrste Klebsiella sp. izolovan iz oligomineralne vode za piće kaptiranog izvora na Vlasini. Zbog podataka da pojedine vrste roda Klebsiella mogu imati medicinski značaj, kao oportuni patogeni, izvršili smo biohemijsku i molekularnu karakterizaciju soja ST5. Biohemijska karakterizacija soja urađena je pomoću manualnog identifikacionog sistema API 20E (bioMèrieux). Procena identifikacije dobijena je na osnovu baze podataka identifikacionog softvera (Apilabplus V 3.3.3 bioMèrieux). Molekularna karakterizacija urađena je PCR amplifikacijom 16S rDNK i sekvenciranjem metodom 'Thermal cycling sequencing'. Poređenje sekvence sa bankom podataka gena NCBI za 16S rRNK dobijena je najveća sličnost (95%) identičnosti ispitivanog soja sa sojem Klebsiella sp. označenim kao LB-2 (gi|110931878|gb|DQ831003.1) izolovanog u Kini. Biohemijska i molekularna karakterizacija dokazala je autohtoni karakter soja ST5 vrste Klebsiella sp.
AB  - The subject of this biochemical and molecular characterization was the dominant strain ST5 of Klebsiella sp. isolated from oligomineral drinking water from the capped spring in the Vlasina mountaineous region. Former data have proven that some types of the genus Klebsiella may be of medical importance as opportunistic pathogens which was the reason why the biochemical and molecular characterization of the strain ST5 had been done. The biochemical characterization of the ST5 strain was performed by means of the manual identification system API 20 E (bioMèrieux). The identification was based on the data base of the identification software (Apilabplus V 3.3.3 bioMèrieux). The molecular characterization was performed by means of the PCR amplification of 16S rDNK and the sequencing method 'Thermal cycling sequencing'. The comparison of the NCBI gene bank data for 16S rRNK resulted in the highest level of similarity, i.e. 95% of similarity for the observed genus compared to the genus Klebsiella sp. marked as LB-2 (gi|110931878|gb|DQ831003.1) isolated in China. The biochemical and molecular characterization proved the autochthonous character of the ST5 strain of Klebsiella sp.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Karakterizacija Klebsiella sp. izolovane iz izvorske oligomineralne vode za piće
T1  - The characterization of Klebsiella sp. isolated from oligomineral drinking water springs
EP  - 45
IS  - 1
SP  - 37
VL  - 72
UR  - https://hdl.handle.net/21.15107/rcub_imagine_500
ER  - 

@article{
author = "Tasić, Srđan and Obradović, Dragojlo and Kojić, Milan and Tasić, Irena",
year = "2011",
abstract = "Predmet biohemijske i molekularne karakterizacije bio je dominantan soj ST5 vrste Klebsiella sp. izolovan iz oligomineralne vode za piće kaptiranog izvora na Vlasini. Zbog podataka da pojedine vrste roda Klebsiella mogu imati medicinski značaj, kao oportuni patogeni, izvršili smo biohemijsku i molekularnu karakterizaciju soja ST5. Biohemijska karakterizacija soja urađena je pomoću manualnog identifikacionog sistema API 20E (bioMèrieux). Procena identifikacije dobijena je na osnovu baze podataka identifikacionog softvera (Apilabplus V 3.3.3 bioMèrieux). Molekularna karakterizacija urađena je PCR amplifikacijom 16S rDNK i sekvenciranjem metodom 'Thermal cycling sequencing'. Poređenje sekvence sa bankom podataka gena NCBI za 16S rRNK dobijena je najveća sličnost (95%) identičnosti ispitivanog soja sa sojem Klebsiella sp. označenim kao LB-2 (gi|110931878|gb|DQ831003.1) izolovanog u Kini. Biohemijska i molekularna karakterizacija dokazala je autohtoni karakter soja ST5 vrste Klebsiella sp., The subject of this biochemical and molecular characterization was the dominant strain ST5 of Klebsiella sp. isolated from oligomineral drinking water from the capped spring in the Vlasina mountaineous region. Former data have proven that some types of the genus Klebsiella may be of medical importance as opportunistic pathogens which was the reason why the biochemical and molecular characterization of the strain ST5 had been done. The biochemical characterization of the ST5 strain was performed by means of the manual identification system API 20 E (bioMèrieux). The identification was based on the data base of the identification software (Apilabplus V 3.3.3 bioMèrieux). The molecular characterization was performed by means of the PCR amplification of 16S rDNK and the sequencing method 'Thermal cycling sequencing'. The comparison of the NCBI gene bank data for 16S rRNK resulted in the highest level of similarity, i.e. 95% of similarity for the observed genus compared to the genus Klebsiella sp. marked as LB-2 (gi|110931878|gb|DQ831003.1) isolated in China. The biochemical and molecular characterization proved the autochthonous character of the ST5 strain of Klebsiella sp.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Karakterizacija Klebsiella sp. izolovane iz izvorske oligomineralne vode za piće, The characterization of Klebsiella sp. isolated from oligomineral drinking water springs",
pages = "45-37",
number = "1",
volume = "72",
url = "https://hdl.handle.net/21.15107/rcub_imagine_500"
}

Tasić, S., Obradović, D., Kojić, M.,& Tasić, I.. (2011). Karakterizacija Klebsiella sp. izolovane iz izvorske oligomineralne vode za piće. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 72(1), 37-45.
https://hdl.handle.net/21.15107/rcub_imagine_500

Tasić S, Obradović D, Kojić M, Tasić I. Karakterizacija Klebsiella sp. izolovane iz izvorske oligomineralne vode za piće. in Journal of Scientific Agricultural Research. 2011;72(1):37-45.
https://hdl.handle.net/21.15107/rcub_imagine_500 .

Tasić, Srđan, Obradović, Dragojlo, Kojić, Milan, Tasić, Irena, "Karakterizacija Klebsiella sp. izolovane iz izvorske oligomineralne vode za piće" in Journal of Scientific Agricultural Research, 72, no. 1 (2011):37-45,
https://hdl.handle.net/21.15107/rcub_imagine_500 .