TY  - JOUR
AU  - Virijević, Katarina
AU  - Živanović, Marko N.
AU  - Nikolić, Dalibor
AU  - Milivojević, Nevena
AU  - Pavić, Jelena
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Dragačević, Luka
AU  - Thurner, Philipp J.
AU  - Rufin, Manuel
AU  - Andriotis, Orestis G.
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Filipović, Nenad
PY  - 2024
UR  - https://doi.org/10.1021/acsami.4c03266
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2360
AB  - Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.
PB  - American Chemical Society
T2  - ACS Applied Materials & Interfaces
T1  - AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing
DO  - 10.1021/acsami.4c03266
ER  - 

@article{
author = "Virijević, Katarina and Živanović, Marko N. and Nikolić, Dalibor and Milivojević, Nevena and Pavić, Jelena and Morić, Ivana and Šenerović, Lidija and Dragačević, Luka and Thurner, Philipp J. and Rufin, Manuel and Andriotis, Orestis G. and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Filipović, Nenad",
year = "2024",
abstract = "Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials & Interfaces",
title = "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing",
doi = "10.1021/acsami.4c03266"
}

Virijević, K., Živanović, M. N., Nikolić, D., Milivojević, N., Pavić, J., Morić, I., Šenerović, L., Dragačević, L., Thurner, P. J., Rufin, M., Andriotis, O. G., Ljujić, B., Miletić Kovačević, M., Papić, M.,& Filipović, N.. (2024). AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces
American Chemical Society..
https://doi.org/10.1021/acsami.4c03266

Virijević K, Živanović MN, Nikolić D, Milivojević N, Pavić J, Morić I, Šenerović L, Dragačević L, Thurner PJ, Rufin M, Andriotis OG, Ljujić B, Miletić Kovačević M, Papić M, Filipović N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces. 2024;.
doi:10.1021/acsami.4c03266 .

Virijević, Katarina, Živanović, Marko N., Nikolić, Dalibor, Milivojević, Nevena, Pavić, Jelena, Morić, Ivana, Šenerović, Lidija, Dragačević, Luka, Thurner, Philipp J., Rufin, Manuel, Andriotis, Orestis G., Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Filipović, Nenad, "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing" in ACS Applied Materials & Interfaces (2024),
https://doi.org/10.1021/acsami.4c03266 . .

TY  - CONF
AU  - Milivojević, Nevena
AU  - Prosenc Zmrzljak, Uršula
AU  - Ljujić, Biljana
AU  - Đorđević, Valentina
AU  - Gazdić Janković, Marina
AU  - Živanović, Marko
AU  - Puač, Feđa
AU  - Ivanović, Miloš
AU  - Filipović, Nenad
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1986
AB  - Whole 3’ transcriptome profiling at the single cell level opens up new abilities for researchers to
answer complex questions. Thousands of individual cells per sample are Barcoded separately to
index the transcriptome of each cell individually. It is done by partitioning thousands of cells into
nanoliter-scale Gel Beads-in-emulsion (GEMs), where cells are delivered at a limiting dilution, such
that the majority (~90-99%) of generated GEMs contain no cell. The 16 bp 10x Barcode and 12 bp
UMI are encoded in Read 1, while the poly(dT) primers are used in this protocol for generating Single
Cell 3’ Gene Expression libraries. After GEM generation, copartitioned cells are lysed and reverse
transcription (RT) was performed after which all cDNA from single cell share a common Barcode.
Full-length cDNA was amplified via PCR to generate sufficient mass for library construction. This is
followed by enzymatic fragmentation and size selection to optimize the cDNA amplicon size. Library
construction was finished via End Repair, A-tailing, Adaptor Ligation, and PCR. P5, P7, i7 and i5
sample index, and TruSeq Read 2 (read 2 primer sequence) were added. TruSeq Read 1 and TruSeq
Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing. The library
prepared in this way, containing the P5 and P7 primers, is ready for Illumina amplification.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Single cell 3’ transcriptome profiling
EP  - 45
SP  - 45
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1986
ER  - 

@conference{
author = "Milivojević, Nevena and Prosenc Zmrzljak, Uršula and Ljujić, Biljana and Đorđević, Valentina and Gazdić Janković, Marina and Živanović, Marko and Puač, Feđa and Ivanović, Miloš and Filipović, Nenad",
year = "2023",
abstract = "Whole 3’ transcriptome profiling at the single cell level opens up new abilities for researchers to
answer complex questions. Thousands of individual cells per sample are Barcoded separately to
index the transcriptome of each cell individually. It is done by partitioning thousands of cells into
nanoliter-scale Gel Beads-in-emulsion (GEMs), where cells are delivered at a limiting dilution, such
that the majority (~90-99%) of generated GEMs contain no cell. The 16 bp 10x Barcode and 12 bp
UMI are encoded in Read 1, while the poly(dT) primers are used in this protocol for generating Single
Cell 3’ Gene Expression libraries. After GEM generation, copartitioned cells are lysed and reverse
transcription (RT) was performed after which all cDNA from single cell share a common Barcode.
Full-length cDNA was amplified via PCR to generate sufficient mass for library construction. This is
followed by enzymatic fragmentation and size selection to optimize the cDNA amplicon size. Library
construction was finished via End Repair, A-tailing, Adaptor Ligation, and PCR. P5, P7, i7 and i5
sample index, and TruSeq Read 2 (read 2 primer sequence) were added. TruSeq Read 1 and TruSeq
Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing. The library
prepared in this way, containing the P5 and P7 primers, is ready for Illumina amplification.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Single cell 3’ transcriptome profiling",
pages = "45-45",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1986"
}

Milivojević, N., Prosenc Zmrzljak, U., Ljujić, B., Đorđević, V., Gazdić Janković, M., Živanović, M., Puač, F., Ivanović, M.,& Filipović, N.. (2023). Single cell 3’ transcriptome profiling. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 45-45.
https://hdl.handle.net/21.15107/rcub_imagine_1986

Milivojević N, Prosenc Zmrzljak U, Ljujić B, Đorđević V, Gazdić Janković M, Živanović M, Puač F, Ivanović M, Filipović N. Single cell 3’ transcriptome profiling. in 4th Belgrade Bioinformatics Conference. 2023;4:45-45.
https://hdl.handle.net/21.15107/rcub_imagine_1986 .

Milivojević, Nevena, Prosenc Zmrzljak, Uršula, Ljujić, Biljana, Đorđević, Valentina, Gazdić Janković, Marina, Živanović, Marko, Puač, Feđa, Ivanović, Miloš, Filipović, Nenad, "Single cell 3’ transcriptome profiling" in 4th Belgrade Bioinformatics Conference, 4 (2023):45-45,
https://hdl.handle.net/21.15107/rcub_imagine_1986 .

TY  - CONF
AU  - Vulović, Aleksandra
AU  - Geroski, Tijana
AU  - Filipović, Nenad
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1979
AB  - Experimental studies have shown that lower shear stress values lead to better femoral
bone – hip implant connection. Numerical simulations have provided option to reduce
the number of experimental studies through analysis of different hip implant surface
topographies. However, this approach takes time as there are different model parameters
that should be considered in order to understand how they affect the obtained shear
stress values. The use of classification algorithms is an approach that could reduce the
time required for simulation by providing information about models with biggest potential.
Eleven model parameters related to model and surface topography were considered
in combination with four classification algorithms - Support Vector Machines (SVM), K
- Nearest Neighbor (KNN), Decision Tree (DT), and Random Forest (RF). The considered
parameters were: Number of half-cylinders lengthwise (>0); Number of half-cylinder rows
(≥0); Half cylinders added or removed from the surface (0 – removed; 1 - added); Distance
between half-cylinders lengthwise (≥0); Distance between half-cylinders widthwise
(≥0); Number of different radius values (1 or 2); Radius 1 value (>0); Radius 2 value (≥0);
Distance from the edge where loading is located (≥0); Distance from the other edge of the
model (≥0); Model includes trabecular bone (0 – not included; 1 - included). The aim was
to apply previously mentioned algorithms to obtain information if the maximum shear
stress value was above or below user-defined threshold. The obtained results show that
this approach can be useful to obtain preliminary information about models that should
be numerically analyzed.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Application of classification algorithms for hip implant surface topographies
EP  - 41
SP  - 41
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1979
ER  - 

@conference{
author = "Vulović, Aleksandra and Geroski, Tijana and Filipović, Nenad",
year = "2023",
abstract = "Experimental studies have shown that lower shear stress values lead to better femoral
bone – hip implant connection. Numerical simulations have provided option to reduce
the number of experimental studies through analysis of different hip implant surface
topographies. However, this approach takes time as there are different model parameters
that should be considered in order to understand how they affect the obtained shear
stress values. The use of classification algorithms is an approach that could reduce the
time required for simulation by providing information about models with biggest potential.
Eleven model parameters related to model and surface topography were considered
in combination with four classification algorithms - Support Vector Machines (SVM), K
- Nearest Neighbor (KNN), Decision Tree (DT), and Random Forest (RF). The considered
parameters were: Number of half-cylinders lengthwise (>0); Number of half-cylinder rows
(≥0); Half cylinders added or removed from the surface (0 – removed; 1 - added); Distance
between half-cylinders lengthwise (≥0); Distance between half-cylinders widthwise
(≥0); Number of different radius values (1 or 2); Radius 1 value (>0); Radius 2 value (≥0);
Distance from the edge where loading is located (≥0); Distance from the other edge of the
model (≥0); Model includes trabecular bone (0 – not included; 1 - included). The aim was
to apply previously mentioned algorithms to obtain information if the maximum shear
stress value was above or below user-defined threshold. The obtained results show that
this approach can be useful to obtain preliminary information about models that should
be numerically analyzed.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Application of classification algorithms for hip implant surface topographies",
pages = "41-41",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1979"
}

Vulović, A., Geroski, T.,& Filipović, N.. (2023). Application of classification algorithms for hip implant surface topographies. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 41-41.
https://hdl.handle.net/21.15107/rcub_imagine_1979

Vulović A, Geroski T, Filipović N. Application of classification algorithms for hip implant surface topographies. in 4th Belgrade Bioinformatics Conference. 2023;4:41-41.
https://hdl.handle.net/21.15107/rcub_imagine_1979 .

Vulović, Aleksandra, Geroski, Tijana, Filipović, Nenad, "Application of classification algorithms for hip implant surface topographies" in 4th Belgrade Bioinformatics Conference, 4 (2023):41-41,
https://hdl.handle.net/21.15107/rcub_imagine_1979 .

TY  - CONF
AU  - Filipović, Nenad
AU  - Exarchos, Themis
AU  - Jakovljević, Đorđe
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1952
AB  - In silico clinical trials are a new paradigm for development of a new drug and medical device.
SILICOFCM project is multiscale modeling of familial cardiomyopathy which considers
a comprehensive list of patient specific features as genetic, biological, pharmacologic,
clinical, imaging and cellular aspects.
The 3D deformable-body represents the left and right ventricle of the heart. Blood flow
is modeled during the filling phase by applying the fluid-solid interaction method. The
ventricle wall is modeled by 3D brick 8-node solid elements, with fibers that have threedimensional
direction. The Navier-Stokes equations are solved using the ALE formulation
for fluid with large displacements of the boundary. The ventricle wall model is simulated
by the muscle material model. Muscle fiber orientation is defined by direction vector in 3D
prescribed through input data. The outlet blood pressure is used as the boundary condition.
At the same time, the wall muscle fibers are activated according to the activation function
taken from specific patient measurements.
Computational Platform for Multiscale Modelling in biomedical engineering is results of
SGABU project that is served as an educational tool for students and researchers. The
platform integrates already developed solutions and various datasets related to cancer,
cardiovascular, bone disorders and tissue engineering into one multiscale platform. This
will enable further validation and parameterization of models, creation of environment for
future trends, e.g. in silico clinical trials, virtual surgery, development of prediction models.
InSilc project is devoted to in silico mechanical stent testing within ISO 25539 standards
and in silico stent deployment for metallic and biodegradable material.
In-silico projects will connect basic experimental research with clinical study and
bioinformatics, data mining and image processing tools using very advanced computer
models for drug, stent and patient database in order to reduce animal and clinical studies.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Computational bioengineering for heart disease
EP  - 17
SP  - 17
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1952
ER  - 

@conference{
author = "Filipović, Nenad and Exarchos, Themis and Jakovljević, Đorđe",
year = "2023",
abstract = "In silico clinical trials are a new paradigm for development of a new drug and medical device.
SILICOFCM project is multiscale modeling of familial cardiomyopathy which considers
a comprehensive list of patient specific features as genetic, biological, pharmacologic,
clinical, imaging and cellular aspects.
The 3D deformable-body represents the left and right ventricle of the heart. Blood flow
is modeled during the filling phase by applying the fluid-solid interaction method. The
ventricle wall is modeled by 3D brick 8-node solid elements, with fibers that have threedimensional
direction. The Navier-Stokes equations are solved using the ALE formulation
for fluid with large displacements of the boundary. The ventricle wall model is simulated
by the muscle material model. Muscle fiber orientation is defined by direction vector in 3D
prescribed through input data. The outlet blood pressure is used as the boundary condition.
At the same time, the wall muscle fibers are activated according to the activation function
taken from specific patient measurements.
Computational Platform for Multiscale Modelling in biomedical engineering is results of
SGABU project that is served as an educational tool for students and researchers. The
platform integrates already developed solutions and various datasets related to cancer,
cardiovascular, bone disorders and tissue engineering into one multiscale platform. This
will enable further validation and parameterization of models, creation of environment for
future trends, e.g. in silico clinical trials, virtual surgery, development of prediction models.
InSilc project is devoted to in silico mechanical stent testing within ISO 25539 standards
and in silico stent deployment for metallic and biodegradable material.
In-silico projects will connect basic experimental research with clinical study and
bioinformatics, data mining and image processing tools using very advanced computer
models for drug, stent and patient database in order to reduce animal and clinical studies.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Computational bioengineering for heart disease",
pages = "17-17",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1952"
}

Filipović, N., Exarchos, T.,& Jakovljević, Đ.. (2023). Computational bioengineering for heart disease. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 17-17.
https://hdl.handle.net/21.15107/rcub_imagine_1952

Filipović N, Exarchos T, Jakovljević Đ. Computational bioengineering for heart disease. in 4th Belgrade Bioinformatics Conference. 2023;4:17-17.
https://hdl.handle.net/21.15107/rcub_imagine_1952 .

Filipović, Nenad, Exarchos, Themis, Jakovljević, Đorđe, "Computational bioengineering for heart disease" in 4th Belgrade Bioinformatics Conference, 4 (2023):17-17,
https://hdl.handle.net/21.15107/rcub_imagine_1952 .

TY  - CONF
AU  - Virijević, Katarina
AU  - Živanović, Marko
AU  - Gazdić Janković, Marina
AU  - Ramović Hamzagić, Amra
AU  - Milivojević, Nevena
AU  - Pecić, Katarina
AU  - Šeklić, Dragana
AU  - Jovanović, Milena
AU  - Kastratović, Nikolina
AU  - Mirić, Ana
AU  - Đukić, Tijana
AU  - Petrović, Ivica
AU  - Jurišić, Vladimir
AU  - Ljujić, Biljana
AU  - Filipović, Nenad
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2010
AB  - Biomedicine is a multidisciplinary branch of science that requires a clear approach to the
study and analysis of various life processes necessary for a deeper understanding of
human health. This research focuses on the use of numerical simulations with the aim of an
improved comprehension of cancer viability and apoptosis during treatment with commercial
chemotherapeutic agents. In recent times, the usage of numerical models was successfully
applied to predict the behavior of tumors. This study includes a wide range of numerical results
that have been obtained by examining cell viability in real-time, determining the type of cell
death and the genetic factors that control these processes. The results of the in vitro test were
used to develop a numerical model that provides a new perspective on the proposed problem.
In this study, colon, and breast cancer cell lines (HCT-116 and MDA-MB-231), as well as healthy
lung fibroblast cell line (MRC-5) were treated with commercial chemotherapeutic agents. The
obtained results showed a decrease in viability and the occurrence of predominantly late
apoptosis upon treatment, as well as a strong correlation between parameters. A mathematical
model was developed and used to gain a better understanding of the investigated processes.
This method can accurately simulate the behavior of cancer cells and reliably predict their
growth.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Numerical and Biological Modeling Approach in the Analysis of the Cancer Viability and Apoptosis
EP  - 70
SP  - 70
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2010
ER  - 

@conference{
author = "Virijević, Katarina and Živanović, Marko and Gazdić Janković, Marina and Ramović Hamzagić, Amra and Milivojević, Nevena and Pecić, Katarina and Šeklić, Dragana and Jovanović, Milena and Kastratović, Nikolina and Mirić, Ana and Đukić, Tijana and Petrović, Ivica and Jurišić, Vladimir and Ljujić, Biljana and Filipović, Nenad",
year = "2023",
abstract = "Biomedicine is a multidisciplinary branch of science that requires a clear approach to the
study and analysis of various life processes necessary for a deeper understanding of
human health. This research focuses on the use of numerical simulations with the aim of an
improved comprehension of cancer viability and apoptosis during treatment with commercial
chemotherapeutic agents. In recent times, the usage of numerical models was successfully
applied to predict the behavior of tumors. This study includes a wide range of numerical results
that have been obtained by examining cell viability in real-time, determining the type of cell
death and the genetic factors that control these processes. The results of the in vitro test were
used to develop a numerical model that provides a new perspective on the proposed problem.
In this study, colon, and breast cancer cell lines (HCT-116 and MDA-MB-231), as well as healthy
lung fibroblast cell line (MRC-5) were treated with commercial chemotherapeutic agents. The
obtained results showed a decrease in viability and the occurrence of predominantly late
apoptosis upon treatment, as well as a strong correlation between parameters. A mathematical
model was developed and used to gain a better understanding of the investigated processes.
This method can accurately simulate the behavior of cancer cells and reliably predict their
growth.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Numerical and Biological Modeling Approach in the Analysis of the Cancer Viability and Apoptosis",
pages = "70-70",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2010"
}

Virijević, K., Živanović, M., Gazdić Janković, M., Ramović Hamzagić, A., Milivojević, N., Pecić, K., Šeklić, D., Jovanović, M., Kastratović, N., Mirić, A., Đukić, T., Petrović, I., Jurišić, V., Ljujić, B.,& Filipović, N.. (2023). Numerical and Biological Modeling Approach in the Analysis of the Cancer Viability and Apoptosis. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 70-70.
https://hdl.handle.net/21.15107/rcub_imagine_2010

Virijević K, Živanović M, Gazdić Janković M, Ramović Hamzagić A, Milivojević N, Pecić K, Šeklić D, Jovanović M, Kastratović N, Mirić A, Đukić T, Petrović I, Jurišić V, Ljujić B, Filipović N. Numerical and Biological Modeling Approach in the Analysis of the Cancer Viability and Apoptosis. in 4th Belgrade Bioinformatics Conference. 2023;4:70-70.
https://hdl.handle.net/21.15107/rcub_imagine_2010 .

Virijević, Katarina, Živanović, Marko, Gazdić Janković, Marina, Ramović Hamzagić, Amra, Milivojević, Nevena, Pecić, Katarina, Šeklić, Dragana, Jovanović, Milena, Kastratović, Nikolina, Mirić, Ana, Đukić, Tijana, Petrović, Ivica, Jurišić, Vladimir, Ljujić, Biljana, Filipović, Nenad, "Numerical and Biological Modeling Approach in the Analysis of the Cancer Viability and Apoptosis" in 4th Belgrade Bioinformatics Conference, 4 (2023):70-70,
https://hdl.handle.net/21.15107/rcub_imagine_2010 .

TY  - DATA
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Tomić, Nina
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Stampfl, Juergen
AU  - Boccaccin, Aldo
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1774
PB  - SAGE
T2  - Nanotechnology in Biomaterials
T1  - Supplementary data for the article: Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J., & Boccaccini, A. R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications, 36(10), 1800–1811. [https://doi.org/10.1177/08853282211073731]
IS  - 10
VL  - 36
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1774
ER  - 

@misc{
author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccin, Aldo",
year = "2022",
publisher = "SAGE",
journal = "Nanotechnology in Biomaterials",
title = "Supplementary data for the article: Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J., & Boccaccini, A. R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications, 36(10), 1800–1811. [https://doi.org/10.1177/08853282211073731]",
number = "10",
volume = "36",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1774"
}

Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccin, A.. (2022). Supplementary data for the article: Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J., & Boccaccini, A. R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications, 36(10), 1800–1811. [https://doi.org/10.1177/08853282211073731]. in Nanotechnology in Biomaterials
SAGE., 36(10).
https://hdl.handle.net/21.15107/rcub_imagine_1774

Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccin A. Supplementary data for the article: Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J., & Boccaccini, A. R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications, 36(10), 1800–1811. [https://doi.org/10.1177/08853282211073731]. in Nanotechnology in Biomaterials. 2022;36(10).
https://hdl.handle.net/21.15107/rcub_imagine_1774 .

Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccin, Aldo, "Supplementary data for the article: Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J., & Boccaccini, A. R. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. Journal of Biomaterials Applications, 36(10), 1800–1811. [https://doi.org/10.1177/08853282211073731]" in Nanotechnology in Biomaterials, 36, no. 10 (2022),
https://hdl.handle.net/21.15107/rcub_imagine_1774 .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1600
AB  - Aims To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results Chequerboard and time-kill assays were employed to explore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16-256 mu g ml(-1)). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004-0.035) of colistin and SeNPs against colistin-resistant isolates was revealed. Colistin (0.5 or 1 mu g ml(-1)) used in combination with SeNPs (0.5 mu g ml(-1)) was able to reduce initial inoculum during the first 4 h of incubation, in contrast to colistin (0.5, 1 or 2 mu g ml(-1)) alone. Conclusions These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
EP  - 1206
IS  - 3
SP  - 1197
VL  - 133
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2022",
abstract = "Aims To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results Chequerboard and time-kill assays were employed to explore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16-256 mu g ml(-1)). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004-0.035) of colistin and SeNPs against colistin-resistant isolates was revealed. Colistin (0.5 or 1 mu g ml(-1)) used in combination with SeNPs (0.5 mu g ml(-1)) was able to reduce initial inoculum during the first 4 h of incubation, in contrast to colistin (0.5, 1 or 2 mu g ml(-1)) alone. Conclusions These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
pages = "1206-1197",
number = "3",
volume = "133",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
Wiley, Hoboken., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković MT. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Ušjak, Dušan
AU  - Milenković, Marina T.
AU  - Zheng, Kai
AU  - Liverani, Liliana
AU  - Boccaccini, Aldo R.
AU  - Stevanović, Magdalena M.
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2020.624621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1772
AB  - Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.
PB  - Frontiers
T2  - Frontiers in Bioengineering and Biotechnology
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure
VL  - 8
DO  - 10.3389/fbioe.2020.624621
ER  - 

@article{
author = "Filipović, Nenad and Ušjak, Dušan and Milenković, Marina T. and Zheng, Kai and Liverani, Liliana and Boccaccini, Aldo R. and Stevanović, Magdalena M.",
year = "2021",
abstract = "Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.",
publisher = "Frontiers",
journal = "Frontiers in Bioengineering and Biotechnology, Frontiers in Bioengineering and Biotechnology",
title = "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure",
volume = "8",
doi = "10.3389/fbioe.2020.624621"
}

Filipović, N., Ušjak, D., Milenković, M. T., Zheng, K., Liverani, L., Boccaccini, A. R.,& Stevanović, M. M.. (2021). Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology
Frontiers., 8.
https://doi.org/10.3389/fbioe.2020.624621

Filipović N, Ušjak D, Milenković MT, Zheng K, Liverani L, Boccaccini AR, Stevanović MM. Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology. 2021;8.
doi:10.3389/fbioe.2020.624621 .

Filipović, Nenad, Ušjak, Dušan, Milenković, Marina T., Zheng, Kai, Liverani, Liliana, Boccaccini, Aldo R., Stevanović, Magdalena M., "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure" in Frontiers in Bioengineering and Biotechnology, 8 (2021),
https://doi.org/10.3389/fbioe.2020.624621 . .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1613
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
EP  - 789
SP  - 776
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 

@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "789-776",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}

Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C
Elsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073

Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073 .

Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science and Engineering C, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 . .

TY  - DATA
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1615
AB  - 1. Experimental details for ICP-OES measurements; 1.1. Instrumental and operating conditions; 1.2.Solutions and Reagents; 1.3. Microwave assisted acid digestion; 1.4. Calibration curve 2. Experimental details for biocompatibility investigations of PCL/SeNPs; 2.1. Cell culture; 2.2.Determining citotoxicity of samples - MTT assay; 2.3. Determination of intracellular reactive oxygen species formation – DCFH-DA assay; 2.4. DNA damage (comet assay) Figure 1. SEM image of blank PCL microspheres Figure 2. XRD pattern of commercial PGA used in experiments Figure 3. Interaction with PCL/SeNPs in vivo by infiltrating cells. PCL/SeNPs (4mg/animal) were injected into sterile polyvinyl sponges implanted subcutaneously. The infiltrating cells were collected from the sponges after 3h and stained to anti-CD45/IgG Alexa 488 (Green) and Syto59 nuclear stain. PCL/SeNPs were detected as brightly scattering particles sized about 1-4 μm after 546nm laser excitation either intracellularly within granulocytes (A) or extracellularly (B). Note that some cells expressed strongly CD45 on the membrane and the cytoplasm, whereas others displayed a weak membrane expression and a strong expression in the granular ER at the nucleus level. Table 1. Melting temperatures Tm and corresponding enthalpies (heat) of fusion ΔHf of PCL/SeNPs samples taken after different time from different degradation mediums Table 2. Melting temperatures and corresponding enthalpies of PCL/SeNPs samples taken after different degradation periods from P. aeruginosa CFE medium
T2  - Materials Science and Engineering C
T1  - Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1615
ER  - 

@misc{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "1. Experimental details for ICP-OES measurements; 1.1. Instrumental and operating conditions; 1.2.Solutions and Reagents; 1.3. Microwave assisted acid digestion; 1.4. Calibration curve 2. Experimental details for biocompatibility investigations of PCL/SeNPs; 2.1. Cell culture; 2.2.Determining citotoxicity of samples - MTT assay; 2.3. Determination of intracellular reactive oxygen species formation – DCFH-DA assay; 2.4. DNA damage (comet assay) Figure 1. SEM image of blank PCL microspheres Figure 2. XRD pattern of commercial PGA used in experiments Figure 3. Interaction with PCL/SeNPs in vivo by infiltrating cells. PCL/SeNPs (4mg/animal) were injected into sterile polyvinyl sponges implanted subcutaneously. The infiltrating cells were collected from the sponges after 3h and stained to anti-CD45/IgG Alexa 488 (Green) and Syto59 nuclear stain. PCL/SeNPs were detected as brightly scattering particles sized about 1-4 μm after 546nm laser excitation either intracellularly within granulocytes (A) or extracellularly (B). Note that some cells expressed strongly CD45 on the membrane and the cytoplasm, whereas others displayed a weak membrane expression and a strong expression in the granular ER at the nucleus level. Table 1. Melting temperatures Tm and corresponding enthalpies (heat) of fusion ΔHf of PCL/SeNPs samples taken after different time from different degradation mediums Table 2. Melting temperatures and corresponding enthalpies of PCL/SeNPs samples taken after different degradation periods from P. aeruginosa CFE medium",
journal = "Materials Science and Engineering C",
title = "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1615"
}

Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073. in Materials Science and Engineering C.
https://hdl.handle.net/21.15107/rcub_imagine_1615

Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073. in Materials Science and Engineering C. 2019;.
https://hdl.handle.net/21.15107/rcub_imagine_1615 .

Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073" in Materials Science and Engineering C (2019),
https://hdl.handle.net/21.15107/rcub_imagine_1615 .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Razić, Slavica
AU  - Jeremić, Sanja
AU  - Filipić, Metka
AU  - Zegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1292
AB  - Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.
PB  - Elsevier, Amsterdam
T2  - Materials Science & Engineering C-Materials For Biological Applications
T1  - Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles
EP  - 789
SP  - 776
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 

@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Razić, Slavica and Jeremić, Sanja and Filipić, Metka and Zegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Science & Engineering C-Materials For Biological Applications",
title = "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "789-776",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}

Filipović, N., Veselinović, L., Razić, S., Jeremić, S., Filipić, M., Zegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications
Elsevier, Amsterdam., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073

Filipović N, Veselinović L, Razić S, Jeremić S, Filipić M, Zegura B, Tomić S, Čolić M, Stevanović M. Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073 .

Filipović, Nenad, Veselinović, Ljiljana, Razić, Slavica, Jeremić, Sanja, Filipić, Metka, Zegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science & Engineering C-Materials For Biological Applications, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uros
AU  - Đokić, Jelena
AU  - Stepanović-Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1159
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
VL  - 9
DO  - 10.3389/fphar.2018.00001
ER  - 

@article{
author = "Dinić, Miroslav and Pecikoza, Uros and Đokić, Jelena and Stepanović-Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
volume = "9",
doi = "10.3389/fphar.2018.00001"
}

Dinić, M., Pecikoza, U., Đokić, J., Stepanović-Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J.. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fphar.2018.00001

Dinić M, Pecikoza U, Đokić J, Stepanović-Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology. 2018;9.
doi:10.3389/fphar.2018.00001 .

Dinić, Miroslav, Pecikoza, Uros, Đokić, Jelena, Stepanović-Petrović, Radica, Milenković, Marina, Stevanović, Magdalena, Filipović, Nenad, Begović, Jelena, Golić, Nataša, Lukić, Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" in Frontiers in Pharmacology, 9 (2018),
https://doi.org/10.3389/fphar.2018.00001 . .