TY  - JOUR
AU  - Eshaghi, Mahsa
AU  - Shiran, Behrouz
AU  - Fallahi, Hossein
AU  - Ravash, Rudabeh
AU  - Banović Đeri, Bojana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1218
AB  - Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Genomics
T1  - Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics
EP  - 1372
IS  - 6
SP  - 1360
VL  - 111
DO  - 10.1016/j.ygeno.2018.09.008
ER  - 

@article{
author = "Eshaghi, Mahsa and Shiran, Behrouz and Fallahi, Hossein and Ravash, Rudabeh and Banović Đeri, Bojana",
year = "2019",
abstract = "Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Genomics",
title = "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics",
pages = "1372-1360",
number = "6",
volume = "111",
doi = "10.1016/j.ygeno.2018.09.008"
}

Eshaghi, M., Shiran, B., Fallahi, H., Ravash, R.,& Banović Đeri, B.. (2019). Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics
Academic Press Inc Elsevier Science, San Diego., 111(6), 1360-1372.
https://doi.org/10.1016/j.ygeno.2018.09.008

Eshaghi M, Shiran B, Fallahi H, Ravash R, Banović Đeri B. Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics. 2019;111(6):1360-1372.
doi:10.1016/j.ygeno.2018.09.008 .

Eshaghi, Mahsa, Shiran, Behrouz, Fallahi, Hossein, Ravash, Rudabeh, Banović Đeri, Bojana, "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics" in Genomics, 111, no. 6 (2019):1360-1372,
https://doi.org/10.1016/j.ygeno.2018.09.008 . .