TY  - JOUR
AU  - Ahmadi-Teshniz, Fereshteh
AU  - Shiran, Behrouz
AU  - Mousavi-Fard, Sadegh
AU  - Fallahi, Hossein
AU  - Banović Đeri, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1549
AB  - Background Novel strategies for improvement of ornamental plants and their properties relay on miRNA control of differential plant gene expression modulation. Still, in response to the same abiotic stresses, some conserved miRNA families show different expression patterns in different plant species. In parallel, the use of deep sequencing technologies reveals new levels of complexity of regulatory networks in plants through identification of new miRNAs. Methods and results Fritillaria imperialis plants were collected from their natural habitats in Koohrang, Chaharmahal va Bakhtiari, Iran. Several tissues including stamen, pistil, petal, sepal, leaf, stem, bulb and fruit were collected during three developmental stages (stem elongation, flower development and seed head stages). Using RNAseq and qRT-PCR approach, this research revealed 21 conserved miRNAs, matching 15 miRNA families, in Fritilaria imperialis. Conclusions The expression of seven conserved miRNAs (Fim-miR156b, Fim-miR159, Fim-miR166a-5p, Fim-miR169d-5p, Fim-miR171c, Fim-miR393 and Fim-miR396e-3p) was further investigated in different tissues and three developmental stages, suggesting different roles for these miRNAs during growth and development of crown imperial. Gained knowledge from this research can open the door to find efficient ways to secure crown imperial survival, preservation and utilization and if proven useful may be applied in other plant species as well.
PB  - Springer, Dordrecht
T2  - Molecular Biology Reports
T1  - Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing
EP  - 1132
IS  - 2
SP  - 1121
VL  - 49
DO  - 10.1007/s11033-021-06938-1
ER  - 

@article{
author = "Ahmadi-Teshniz, Fereshteh and Shiran, Behrouz and Mousavi-Fard, Sadegh and Fallahi, Hossein and Banović Đeri, Bojana",
year = "2022",
abstract = "Background Novel strategies for improvement of ornamental plants and their properties relay on miRNA control of differential plant gene expression modulation. Still, in response to the same abiotic stresses, some conserved miRNA families show different expression patterns in different plant species. In parallel, the use of deep sequencing technologies reveals new levels of complexity of regulatory networks in plants through identification of new miRNAs. Methods and results Fritillaria imperialis plants were collected from their natural habitats in Koohrang, Chaharmahal va Bakhtiari, Iran. Several tissues including stamen, pistil, petal, sepal, leaf, stem, bulb and fruit were collected during three developmental stages (stem elongation, flower development and seed head stages). Using RNAseq and qRT-PCR approach, this research revealed 21 conserved miRNAs, matching 15 miRNA families, in Fritilaria imperialis. Conclusions The expression of seven conserved miRNAs (Fim-miR156b, Fim-miR159, Fim-miR166a-5p, Fim-miR169d-5p, Fim-miR171c, Fim-miR393 and Fim-miR396e-3p) was further investigated in different tissues and three developmental stages, suggesting different roles for these miRNAs during growth and development of crown imperial. Gained knowledge from this research can open the door to find efficient ways to secure crown imperial survival, preservation and utilization and if proven useful may be applied in other plant species as well.",
publisher = "Springer, Dordrecht",
journal = "Molecular Biology Reports",
title = "Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing",
pages = "1132-1121",
number = "2",
volume = "49",
doi = "10.1007/s11033-021-06938-1"
}

Ahmadi-Teshniz, F., Shiran, B., Mousavi-Fard, S., Fallahi, H.,& Banović Đeri, B.. (2022). Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing. in Molecular Biology Reports
Springer, Dordrecht., 49(2), 1121-1132.
https://doi.org/10.1007/s11033-021-06938-1

Ahmadi-Teshniz F, Shiran B, Mousavi-Fard S, Fallahi H, Banović Đeri B. Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing. in Molecular Biology Reports. 2022;49(2):1121-1132.
doi:10.1007/s11033-021-06938-1 .

Ahmadi-Teshniz, Fereshteh, Shiran, Behrouz, Mousavi-Fard, Sadegh, Fallahi, Hossein, Banović Đeri, Bojana, "Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing" in Molecular Biology Reports, 49, no. 2 (2022):1121-1132,
https://doi.org/10.1007/s11033-021-06938-1 . .

TY  - JOUR
AU  - Eshaghi, Mahsa
AU  - Shiran, Behrouz
AU  - Fallahi, Hossein
AU  - Ravash, Rudabeh
AU  - Banović Đeri, Bojana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1218
AB  - Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Genomics
T1  - Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics
EP  - 1372
IS  - 6
SP  - 1360
VL  - 111
DO  - 10.1016/j.ygeno.2018.09.008
ER  - 

@article{
author = "Eshaghi, Mahsa and Shiran, Behrouz and Fallahi, Hossein and Ravash, Rudabeh and Banović Đeri, Bojana",
year = "2019",
abstract = "Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Genomics",
title = "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics",
pages = "1372-1360",
number = "6",
volume = "111",
doi = "10.1016/j.ygeno.2018.09.008"
}

Eshaghi, M., Shiran, B., Fallahi, H., Ravash, R.,& Banović Đeri, B.. (2019). Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics
Academic Press Inc Elsevier Science, San Diego., 111(6), 1360-1372.
https://doi.org/10.1016/j.ygeno.2018.09.008

Eshaghi M, Shiran B, Fallahi H, Ravash R, Banović Đeri B. Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics. 2019;111(6):1360-1372.
doi:10.1016/j.ygeno.2018.09.008 .

Eshaghi, Mahsa, Shiran, Behrouz, Fallahi, Hossein, Ravash, Rudabeh, Banović Đeri, Bojana, "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics" in Genomics, 111, no. 6 (2019):1360-1372,
https://doi.org/10.1016/j.ygeno.2018.09.008 . .