TY  - JOUR
AU  - Gasić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1571
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67 degrees C. They were stable at pH 5-9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gasić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Danijela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67 degrees C. They were stable at pH 5-9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gasić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, D.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fmicb.2021.803789

Gasić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić D, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12.
doi:10.3389/fmicb.2021.803789 .

Gasić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Danijela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Vidanović, Dejan
AU  - Bojana, Tešović
AU  - Petrović, Tamaš
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Dudić, Dragana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1869
AB  - Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all
eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile,
pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides
their high changeability, another major reason is their ability to mimic host processes upon entering the host.
Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that
is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid
some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness.
Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+)
methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different
in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal
pattern in concordance with their similar genome organization? Among numerous different methylation
patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant
methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well.
This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are
associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and
eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
C3  - Biologia Serbica
T1  - Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses
IS  - 1 (Special Edition)
SP  - 29
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1869
ER  - 

@conference{
author = "Banović Đeri, Bojana and Vidanović, Dejan and Bojana, Tešović and Petrović, Tamaš and Ristić, Danijela and Vučurović, Ivan and Dudić, Dragana",
year = "2021",
abstract = "Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all
eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile,
pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides
their high changeability, another major reason is their ability to mimic host processes upon entering the host.
Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that
is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid
some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness.
Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+)
methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different
in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal
pattern in concordance with their similar genome organization? Among numerous different methylation
patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant
methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well.
This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are
associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and
eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses",
number = "1 (Special Edition)",
pages = "29",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1869"
}

Banović Đeri, B., Vidanović, D., Bojana, T., Petrović, T., Ristić, D., Vučurović, I.,& Dudić, D.. (2021). Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 43(1 (Special Edition)), 29.
https://hdl.handle.net/21.15107/rcub_imagine_1869

Banović Đeri B, Vidanović D, Bojana T, Petrović T, Ristić D, Vučurović I, Dudić D. Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica. 2021;43(1 (Special Edition)):29.
https://hdl.handle.net/21.15107/rcub_imagine_1869 .

Banović Đeri, Bojana, Vidanović, Dejan, Bojana, Tešović, Petrović, Tamaš, Ristić, Danijela, Vučurović, Ivan, Dudić, Dragana, "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses" in Biologia Serbica, 43, no. 1 (Special Edition) (2021):29,
https://hdl.handle.net/21.15107/rcub_imagine_1869 .