TY  - JOUR
AU  - Josić, D.
AU  - Starović, M.
AU  - Kojić, Snežana
AU  - Pivić, R.
AU  - Stanojković-Sebić, A.
AU  - Zdravković, M.
AU  - Pavlović, S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/819
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia
EP  - 283
IS  - 2
SP  - 283
VL  - 99
DO  - 10.1094/PDIS-08-14-0875-PDN
ER  - 

@article{
author = "Josić, D. and Starović, M. and Kojić, Snežana and Pivić, R. and Stanojković-Sebić, A. and Zdravković, M. and Pavlović, S.",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia",
pages = "283-283",
number = "2",
volume = "99",
doi = "10.1094/PDIS-08-14-0875-PDN"
}

Josić, D., Starović, M., Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, M.,& Pavlović, S.. (2015). Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 283-283.
https://doi.org/10.1094/PDIS-08-14-0875-PDN

Josić D, Starović M, Kojić S, Pivić R, Stanojković-Sebić A, Zdravković M, Pavlović S. Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia. in Plant Disease. 2015;99(2):283-283.
doi:10.1094/PDIS-08-14-0875-PDN .

Josić, D., Starović, M., Kojić, Snežana, Pivić, R., Stanojković-Sebić, A., Zdravković, M., Pavlović, S., "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia" in Plant Disease, 99, no. 2 (2015):283-283,
https://doi.org/10.1094/PDIS-08-14-0875-PDN . .

TY  - JOUR
AU  - Pavlović, S.
AU  - Starović, M.
AU  - Stojanović, S.
AU  - Aleksić, G.
AU  - Kojić, Snežana
AU  - Zdravković, M.
AU  - Josić, D.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/707
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.
EP  - 1152
IS  - 8
SP  - 1152
VL  - 98
DO  - 10.1094/PDIS-01-14-0085-PDN
ER  - 

@article{
author = "Pavlović, S. and Starović, M. and Stojanović, S. and Aleksić, G. and Kojić, Snežana and Zdravković, M. and Josić, D.",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.",
pages = "1152-1152",
number = "8",
volume = "98",
doi = "10.1094/PDIS-01-14-0085-PDN"
}

Pavlović, S., Starović, M., Stojanović, S., Aleksić, G., Kojić, S., Zdravković, M.,& Josić, D.. (2014). The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1152-1152.
https://doi.org/10.1094/PDIS-01-14-0085-PDN

Pavlović S, Starović M, Stojanović S, Aleksić G, Kojić S, Zdravković M, Josić D. The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia.. in Plant Disease. 2014;98(8):1152-1152.
doi:10.1094/PDIS-01-14-0085-PDN .

Pavlović, S., Starović, M., Stojanović, S., Aleksić, G., Kojić, Snežana, Zdravković, M., Josić, D., "The First Report of Stolbur Phytoplasma Associated with Phyllody of Calendula officinalis in Serbia." in Plant Disease, 98, no. 8 (2014):1152-1152,
https://doi.org/10.1094/PDIS-01-14-0085-PDN . .

TY  - JOUR
AU  - Adamović, D.
AU  - Đalović, I.
AU  - Mitrović, P.
AU  - Kojić, Snežana
AU  - Starović, M.
AU  - Purar, B.
AU  - Josić, D.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/720
AB  - Evening primrose (Oenothera biennis L.) is a biennial medicinal, edible, and ornamental plant species. It has attracted great interest for its seed oil that contains gamma linolenic acid, thus distinguishing this plant as a main commercial source of this essential fatty acid (4). This species has been grown as a permanent member of a medicinal plant collection established near Backi Petrovac (northern Serbia) for 22 years. The first disease symptoms were recognized as red spots on leaf rosette in July 2011, spreading gradually during vegetative growth and covering 1/3 to 1/2 of the leaf surface. Symptoms, observed on 16% of the plants (32 of 200) in the second half of May 2012 and on 23% (69 of 300) at the beginning of May 2013, appeared as reddening of lower leaves of flower-bearing stems. Affected plants exhibited stunted growth, while reddening spread over other leaves of flower-bearing stems. In severely affected plants, the flower-bearing stems were poorly developed, frequently forming witches' brooms. For that reason, 30 reddened and 20 symptomless leaves (2 leaves per plant) were sampled in both July 2012 and 2013 and total nucleic acids were extracted. Direct PCR assays were performed using phytoplasma universal primer pair P1/P7 (2) to amplify 1,800-bp fragments (the 16S rRNA gene, the 16S-23S intergenic spacer region, and a part of the 5′ region of the 23S rRNA gene). PCR products were used in nested PCR with primers R16F2n/R2 (2) to amplify 1,200-bp fragments. The identification of phytoplasmas was done using RFLP (restriction fragments length polymorphisms) analyses of R16F2n/R2 amplicons digested with AluI, Kpn I, HpaII, TruI1, or HhaI endonucleases (Thermo Scientific, Lithuania) (2). RFLP patterns were identical to that of STOL reference strain of the 16SrXII-A subgroup, indicating that symptomatic plants were infected with phytoplasma (2). The 16S rDNA nucleotide sequence of representative strain E7 was deposited in GenBank under accession number KF850526. The BLASTn search showed 100% homology to an Iranian strain (KF263684.1) from peach and Serbian strains JQ730742.1 and JQ730750 from valerian and corn, respectively, all belonging to ‘Candidatus Phytoplasma solani’ (Stolbur). Sequencing data confirmed the association of Stolbur phytoplasma with affected O. biennis plants. It has already been reported that phytoplasma infection caused yellows disease of O. biennis (1). Also, the virescence of O. hookeri was associated with phytoplasma strain OAY from aster yellows (AY) group (subgroups 16SrI-B), and selected as the reference strain for the novel taxon ‘Ca. P. asteris’ (3). Here we provide the first report of naturally occurring Stolbur phytoplasma disease of O. biennis in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia
EP  - 841
IS  - 6
SP  - 841
VL  - 98
DO  - 10.1094/PDIS-12-13-1225-PDN
ER  - 

@article{
author = "Adamović, D. and Đalović, I. and Mitrović, P. and Kojić, Snežana and Starović, M. and Purar, B. and Josić, D.",
year = "2014",
abstract = "Evening primrose (Oenothera biennis L.) is a biennial medicinal, edible, and ornamental plant species. It has attracted great interest for its seed oil that contains gamma linolenic acid, thus distinguishing this plant as a main commercial source of this essential fatty acid (4). This species has been grown as a permanent member of a medicinal plant collection established near Backi Petrovac (northern Serbia) for 22 years. The first disease symptoms were recognized as red spots on leaf rosette in July 2011, spreading gradually during vegetative growth and covering 1/3 to 1/2 of the leaf surface. Symptoms, observed on 16% of the plants (32 of 200) in the second half of May 2012 and on 23% (69 of 300) at the beginning of May 2013, appeared as reddening of lower leaves of flower-bearing stems. Affected plants exhibited stunted growth, while reddening spread over other leaves of flower-bearing stems. In severely affected plants, the flower-bearing stems were poorly developed, frequently forming witches' brooms. For that reason, 30 reddened and 20 symptomless leaves (2 leaves per plant) were sampled in both July 2012 and 2013 and total nucleic acids were extracted. Direct PCR assays were performed using phytoplasma universal primer pair P1/P7 (2) to amplify 1,800-bp fragments (the 16S rRNA gene, the 16S-23S intergenic spacer region, and a part of the 5′ region of the 23S rRNA gene). PCR products were used in nested PCR with primers R16F2n/R2 (2) to amplify 1,200-bp fragments. The identification of phytoplasmas was done using RFLP (restriction fragments length polymorphisms) analyses of R16F2n/R2 amplicons digested with AluI, Kpn I, HpaII, TruI1, or HhaI endonucleases (Thermo Scientific, Lithuania) (2). RFLP patterns were identical to that of STOL reference strain of the 16SrXII-A subgroup, indicating that symptomatic plants were infected with phytoplasma (2). The 16S rDNA nucleotide sequence of representative strain E7 was deposited in GenBank under accession number KF850526. The BLASTn search showed 100% homology to an Iranian strain (KF263684.1) from peach and Serbian strains JQ730742.1 and JQ730750 from valerian and corn, respectively, all belonging to ‘Candidatus Phytoplasma solani’ (Stolbur). Sequencing data confirmed the association of Stolbur phytoplasma with affected O. biennis plants. It has already been reported that phytoplasma infection caused yellows disease of O. biennis (1). Also, the virescence of O. hookeri was associated with phytoplasma strain OAY from aster yellows (AY) group (subgroups 16SrI-B), and selected as the reference strain for the novel taxon ‘Ca. P. asteris’ (3). Here we provide the first report of naturally occurring Stolbur phytoplasma disease of O. biennis in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia",
pages = "841-841",
number = "6",
volume = "98",
doi = "10.1094/PDIS-12-13-1225-PDN"
}

Adamović, D., Đalović, I., Mitrović, P., Kojić, S., Starović, M., Purar, B.,& Josić, D.. (2014). First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(6), 841-841.
https://doi.org/10.1094/PDIS-12-13-1225-PDN

Adamović D, Đalović I, Mitrović P, Kojić S, Starović M, Purar B, Josić D. First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia. in Plant Disease. 2014;98(6):841-841.
doi:10.1094/PDIS-12-13-1225-PDN .

Adamović, D., Đalović, I., Mitrović, P., Kojić, Snežana, Starović, M., Purar, B., Josić, D., "First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia" in Plant Disease, 98, no. 6 (2014):841-841,
https://doi.org/10.1094/PDIS-12-13-1225-PDN . .

TY  - JOUR
AU  - Balaz, J.
AU  - Ilicić, R.
AU  - Masirević, S.
AU  - Josić, D.
AU  - Kojić, Snežana
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/775
AB  - Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated from 40 diseased leaves formed white, round, convex, and mucoid colonies on nutrient sucrose agar (NSA). Eight representative strains were aerobic, gram-negative, non-spore-forming rods. All strains produced fluorescent pigment and catalase. In levan-oxidase-potato rot-arginine dihydrolase-tobacco hypersensitivity (LOPAT) tests (3), they induced a hypersensitive reaction in tobacco leaves, did not cause soft rot of potato tubers, and were positive for levan and negative for oxidase and arginine dihydrolase. According to the LOPAT profile, they were classified in the Ia subgroup of pseudomonads (3). Strains hydrolyzed aesculin, but were unable to hydrolyze starch or reduce nitrates to nitrites. Negative reactions were obtained with hydrogen sulfide and indole. Reactions were identical to those of reference strain Pseudomonas syringae pv. syringae CFBP 1582, which was included in all biochemical, physiological, and molecular tests for comparison. To identify the pathogen, PCR and DNA sequencing were employed. Fragments of 752 bp for the syrB gene and 1,040 bp for the syrD gene were amplified from all strains, using B1/B2 and SyD1/SyD2 primer sets, respectively (2). The pathogenicity was tested on seeds and seedlings of oil pumpkin cv. Olinka. Strains were grown for 48 h on nutrient broth (NB) at 28°C and bacterial suspensions of ~108 CFU ml−1 were used for inoculations. Sterile water was used as negative control. Seeds (at the BBCH-1-0 stage) allowed to imbibe water were wounded by needle, immersed in the bacterial suspensions, and maintained in humid petri dishes to allow symptom development. The cotyledons of seedlings at the BBCH-10 stage were inoculated by hypodermic needle and potted plants were maintained at 25 ± 1°C and 75% relative humidity. Symptoms, including dark green, water-soaked spots, appeared 5 to 7 days after inoculation of both seeds and seedlings. The bacterium was re-isolated from spots of all seeds and seedlings tested, fulfilling Koch's postulates (the identity of re-isolated strains was confirmed by pathogenicity, morphology, and biochemical features). No symptoms were observed on controls. 16S rDNA amplicons obtained from representative strain Tk21 and re-isolated strain Tk21R with fD1/rD1 primers (4) were sequenced and deposited in GenBank under accession nos. KF305578 and KF735064, respectively. The sequences showed 100% similarity to each other and P. syringae pv. syringae from pepper (KC816630.1) (China), Ficus carica (JQ071937) (Serbia), and culture-collection ICMP:3023 (HM190217). On the basis of the symptoms, biochemical tests, and 16S rDNA sequence homology, the pathogen was identified as P. syringae pv. syringae. To our knowledge, this is the first report of P. syringae pv. syringae causing bacterial leaf spot on oil pumpkin in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia
EP  - 684
IS  - 5
SP  - 684
VL  - 98
DO  - 10.1094/PDIS-07-13-0714-PDN
ER  - 

@article{
author = "Balaz, J. and Ilicić, R. and Masirević, S. and Josić, D. and Kojić, Snežana",
year = "2014",
abstract = "Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated from 40 diseased leaves formed white, round, convex, and mucoid colonies on nutrient sucrose agar (NSA). Eight representative strains were aerobic, gram-negative, non-spore-forming rods. All strains produced fluorescent pigment and catalase. In levan-oxidase-potato rot-arginine dihydrolase-tobacco hypersensitivity (LOPAT) tests (3), they induced a hypersensitive reaction in tobacco leaves, did not cause soft rot of potato tubers, and were positive for levan and negative for oxidase and arginine dihydrolase. According to the LOPAT profile, they were classified in the Ia subgroup of pseudomonads (3). Strains hydrolyzed aesculin, but were unable to hydrolyze starch or reduce nitrates to nitrites. Negative reactions were obtained with hydrogen sulfide and indole. Reactions were identical to those of reference strain Pseudomonas syringae pv. syringae CFBP 1582, which was included in all biochemical, physiological, and molecular tests for comparison. To identify the pathogen, PCR and DNA sequencing were employed. Fragments of 752 bp for the syrB gene and 1,040 bp for the syrD gene were amplified from all strains, using B1/B2 and SyD1/SyD2 primer sets, respectively (2). The pathogenicity was tested on seeds and seedlings of oil pumpkin cv. Olinka. Strains were grown for 48 h on nutrient broth (NB) at 28°C and bacterial suspensions of ~108 CFU ml−1 were used for inoculations. Sterile water was used as negative control. Seeds (at the BBCH-1-0 stage) allowed to imbibe water were wounded by needle, immersed in the bacterial suspensions, and maintained in humid petri dishes to allow symptom development. The cotyledons of seedlings at the BBCH-10 stage were inoculated by hypodermic needle and potted plants were maintained at 25 ± 1°C and 75% relative humidity. Symptoms, including dark green, water-soaked spots, appeared 5 to 7 days after inoculation of both seeds and seedlings. The bacterium was re-isolated from spots of all seeds and seedlings tested, fulfilling Koch's postulates (the identity of re-isolated strains was confirmed by pathogenicity, morphology, and biochemical features). No symptoms were observed on controls. 16S rDNA amplicons obtained from representative strain Tk21 and re-isolated strain Tk21R with fD1/rD1 primers (4) were sequenced and deposited in GenBank under accession nos. KF305578 and KF735064, respectively. The sequences showed 100% similarity to each other and P. syringae pv. syringae from pepper (KC816630.1) (China), Ficus carica (JQ071937) (Serbia), and culture-collection ICMP:3023 (HM190217). On the basis of the symptoms, biochemical tests, and 16S rDNA sequence homology, the pathogen was identified as P. syringae pv. syringae. To our knowledge, this is the first report of P. syringae pv. syringae causing bacterial leaf spot on oil pumpkin in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia",
pages = "684-684",
number = "5",
volume = "98",
doi = "10.1094/PDIS-07-13-0714-PDN"
}

Balaz, J., Ilicić, R., Masirević, S., Josić, D.,& Kojić, S.. (2014). First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(5), 684-684.
https://doi.org/10.1094/PDIS-07-13-0714-PDN

Balaz J, Ilicić R, Masirević S, Josić D, Kojić S. First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia. in Plant Disease. 2014;98(5):684-684.
doi:10.1094/PDIS-07-13-0714-PDN .

Balaz, J., Ilicić, R., Masirević, S., Josić, D., Kojić, Snežana, "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia" in Plant Disease, 98, no. 5 (2014):684-684,
https://doi.org/10.1094/PDIS-07-13-0714-PDN . .

TY  - JOUR
AU  - Adamović, D.
AU  - Đalović, I.
AU  - Mitrović, P.
AU  - Kojić, Snežana
AU  - Pivić, R.
AU  - Josić, D.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/779
AB  - Peony (Paeonia tenuifolia L.) is a herbaceous perennial plant known for its beautiful and showy flowers. In Serbia it is native to the Deliblato Sands and is used as an ornamental and medicinal plant in folk medicine. This plant species has become a rarity and for that reason peony was introduced into a botanical collection near Backi Petrovac (northern Serbia), where it has been maintained since 1988. Reddening of lower leaves observed on 10% of plants (5 of 50) in the collection at flowering in May 2012 gradually progressed throughout affected plants by the seed maturation stage. Five leaves from each of three reddened and three symptomless plants were sampled at the end of July 2012. Total nucleic acid was extracted separately from individual leaves (30 samples) using the CTAB (cetyltrimethylammonium bromide) method (2). A nested PCR assay using universal primer pairs P1/P7, followed by R16F2n/R16R2 (4), amplified 16S rDNA fragments of 1.8 and 1.2 kb, respectively. DNA from all three reddened plants (15 samples) yielded 1.2-kb amplicons after nested PCRs. Restriction fragment length polymorphism (RFLP) patterns obtained by digestion of nested products with endonucleases AluI, TruI, HpaII, or HhaI (Thermo Scientific, Lithuania) (4) were identical to those of the STOL reference strain included for comparative purposes, indicating that symptoms were consistently associated with plant infection by ‘Ca. Phytoplasma solani’ (Stolbur) phytoplasma. The 16S rDNA amplicons from two peony plants (1.2 kb from B15 and 1.8 from B18) were sequenced (GenBank Accession No. KC960487 and KF614623, respectively). BLAST analysis revealed a 100% identity between the sequences and GenBank sequences of Stolbur phytoplasma, subgroup 16SrXII-A phytoplasma, previously detected in maize (JQ730750) in Serbia and red clover (EU814644.1) in the Czech Republic. Phytoplasma associated diseases of other species of the genus Paeonia (P. lactiflora Pall. and P. suffruticosa Andrews) have been described elsewhere. Disease symptoms on P. lactiflora from Chile were associated with the phytoplasma that belongs to the ribosomal subgroup 16SrVII-A (‘Ca. Phytoplasma fraxini’) (1). Also, Stolbur phytoplasma from the 16SrXII group was detected on P. suffruticosa plants in China, manifesting yellowing symptoms (3). To our knowledge, this is the first report of naturally occurring Stolbur phytoplasma disease of P. tenuifolia L. in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report on Natural Infection of Paeonia tenuifolia by 'Candidatus Phytoplasma solani' in Serbia
EP  - 565
IS  - 4
SP  - 565
VL  - 98
DO  - 10.1094/PDIS-07-13-0702-PDN
ER  - 

@article{
author = "Adamović, D. and Đalović, I. and Mitrović, P. and Kojić, Snežana and Pivić, R. and Josić, D.",
year = "2014",
abstract = "Peony (Paeonia tenuifolia L.) is a herbaceous perennial plant known for its beautiful and showy flowers. In Serbia it is native to the Deliblato Sands and is used as an ornamental and medicinal plant in folk medicine. This plant species has become a rarity and for that reason peony was introduced into a botanical collection near Backi Petrovac (northern Serbia), where it has been maintained since 1988. Reddening of lower leaves observed on 10% of plants (5 of 50) in the collection at flowering in May 2012 gradually progressed throughout affected plants by the seed maturation stage. Five leaves from each of three reddened and three symptomless plants were sampled at the end of July 2012. Total nucleic acid was extracted separately from individual leaves (30 samples) using the CTAB (cetyltrimethylammonium bromide) method (2). A nested PCR assay using universal primer pairs P1/P7, followed by R16F2n/R16R2 (4), amplified 16S rDNA fragments of 1.8 and 1.2 kb, respectively. DNA from all three reddened plants (15 samples) yielded 1.2-kb amplicons after nested PCRs. Restriction fragment length polymorphism (RFLP) patterns obtained by digestion of nested products with endonucleases AluI, TruI, HpaII, or HhaI (Thermo Scientific, Lithuania) (4) were identical to those of the STOL reference strain included for comparative purposes, indicating that symptoms were consistently associated with plant infection by ‘Ca. Phytoplasma solani’ (Stolbur) phytoplasma. The 16S rDNA amplicons from two peony plants (1.2 kb from B15 and 1.8 from B18) were sequenced (GenBank Accession No. KC960487 and KF614623, respectively). BLAST analysis revealed a 100% identity between the sequences and GenBank sequences of Stolbur phytoplasma, subgroup 16SrXII-A phytoplasma, previously detected in maize (JQ730750) in Serbia and red clover (EU814644.1) in the Czech Republic. Phytoplasma associated diseases of other species of the genus Paeonia (P. lactiflora Pall. and P. suffruticosa Andrews) have been described elsewhere. Disease symptoms on P. lactiflora from Chile were associated with the phytoplasma that belongs to the ribosomal subgroup 16SrVII-A (‘Ca. Phytoplasma fraxini’) (1). Also, Stolbur phytoplasma from the 16SrXII group was detected on P. suffruticosa plants in China, manifesting yellowing symptoms (3). To our knowledge, this is the first report of naturally occurring Stolbur phytoplasma disease of P. tenuifolia L. in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report on Natural Infection of Paeonia tenuifolia by 'Candidatus Phytoplasma solani' in Serbia",
pages = "565-565",
number = "4",
volume = "98",
doi = "10.1094/PDIS-07-13-0702-PDN"
}

Adamović, D., Đalović, I., Mitrović, P., Kojić, S., Pivić, R.,& Josić, D.. (2014). First Report on Natural Infection of Paeonia tenuifolia by 'Candidatus Phytoplasma solani' in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(4), 565-565.
https://doi.org/10.1094/PDIS-07-13-0702-PDN

Adamović D, Đalović I, Mitrović P, Kojić S, Pivić R, Josić D. First Report on Natural Infection of Paeonia tenuifolia by 'Candidatus Phytoplasma solani' in Serbia. in Plant Disease. 2014;98(4):565-565.
doi:10.1094/PDIS-07-13-0702-PDN .

Adamović, D., Đalović, I., Mitrović, P., Kojić, Snežana, Pivić, R., Josić, D., "First Report on Natural Infection of Paeonia tenuifolia by 'Candidatus Phytoplasma solani' in Serbia" in Plant Disease, 98, no. 4 (2014):565-565,
https://doi.org/10.1094/PDIS-07-13-0702-PDN . .

TY  - JOUR
AU  - Pavlović, S.
AU  - Starović, M.
AU  - Stojanović, S. D.
AU  - Kojić, Snežana
AU  - Marinković, J.
AU  - Josić, D.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/721
AB  - Chicory (Cichorium intybus, Asteraceae) is a typical Mediterranean plant indigenous to Europe, western Asia, Egypt, and North America (3). It is commonly consumed as a fresh vegetable in salads. In rural areas of Serbia it grows as a weed in crops, but it is used in folk medicine to treat skin disorders due to its antihepatotoxic activity (3). Methanol extracts of chicory leaves showed moderate antibacterial activity against enteric bacteria (3). A phytoplasma-like disease, expressed as proliferation of chicory shoots and flowers, was observed on wild plants for the first time in Obrenovac vicinity (44°40′ N, 20°20′ E) in July 2012. A flattening of the stem with a large number of filamentous leaves, contortion and abnormal growth of flowers on the stem (typical fasciation symptoms) were observed. Diseased plants did not produce seeds. Total DNA was extracted from the leaf midveins of 15 symptomatic and five symptomless plants (4). PCR amplification of 1.5-kb 16S rDNA fragment was performed using DreamTaq Green master mix (Thermo Scientific, Lithuania) and phytoplasma universal primer pairs P1/16S-Sr (1). Products of nested PCR (1.2 kb) were obtained using primer pair R16F2n/R2 (1). Both amplicons were detected in all diseased samples; however, DNA from symptomless samples yielded no amplicons. Restriction fragment length polymorphism (RFLP) analysis of R16F2n/R2 PCR products was performed in independent reactions using four endonucleases (AluI, TruI1, HhaI and HpaII). RFLP patterns from chicory samples were compared to those of Stolbur (STOL), Aster Yellows (AY), Flavescence Dorée-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas (1). All RFLP profiles from the chicory samples were identical to STOL reference strain, indicating that diseased chicory was affected by a phytoplasma that belongs to ‘Candidatus Phytoplasma solani’ (16SrXII-A group). The 16S rDNA sequence of representative sample from symptomatic plant (Vp4) was deposited under accession number KF661322 in NCBI GenBank. It showed 100% identity to KF263684.1 from Iranian peach, JQ730742.1 from Serbian valerian, and JQ730750 from Serbian corn, all belonging to the ‘Ca. P. solani’ taxon. Puna chicory disease on C. intybus associated with a subgroup 16SrV-B of phytoplasma was detected in China (2). This is the first report of the Stolbur phytoplasma associated with fasciation of C. intybus in Serbia and worldwide.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Stolbur Phytoplasma Affecting Cichorium intybus in Serbia.
EP  - 840
IS  - 6
SP  - 839
VL  - 98
DO  - 10.1094/PDIS-09-13-0947-PDN
ER  - 

@article{
author = "Pavlović, S. and Starović, M. and Stojanović, S. D. and Kojić, Snežana and Marinković, J. and Josić, D.",
year = "2014",
abstract = "Chicory (Cichorium intybus, Asteraceae) is a typical Mediterranean plant indigenous to Europe, western Asia, Egypt, and North America (3). It is commonly consumed as a fresh vegetable in salads. In rural areas of Serbia it grows as a weed in crops, but it is used in folk medicine to treat skin disorders due to its antihepatotoxic activity (3). Methanol extracts of chicory leaves showed moderate antibacterial activity against enteric bacteria (3). A phytoplasma-like disease, expressed as proliferation of chicory shoots and flowers, was observed on wild plants for the first time in Obrenovac vicinity (44°40′ N, 20°20′ E) in July 2012. A flattening of the stem with a large number of filamentous leaves, contortion and abnormal growth of flowers on the stem (typical fasciation symptoms) were observed. Diseased plants did not produce seeds. Total DNA was extracted from the leaf midveins of 15 symptomatic and five symptomless plants (4). PCR amplification of 1.5-kb 16S rDNA fragment was performed using DreamTaq Green master mix (Thermo Scientific, Lithuania) and phytoplasma universal primer pairs P1/16S-Sr (1). Products of nested PCR (1.2 kb) were obtained using primer pair R16F2n/R2 (1). Both amplicons were detected in all diseased samples; however, DNA from symptomless samples yielded no amplicons. Restriction fragment length polymorphism (RFLP) analysis of R16F2n/R2 PCR products was performed in independent reactions using four endonucleases (AluI, TruI1, HhaI and HpaII). RFLP patterns from chicory samples were compared to those of Stolbur (STOL), Aster Yellows (AY), Flavescence Dorée-C (FD-C), Poinsettia Branch-Inducing (PoiBI), and Clover Yellow Edge (CYE) phytoplasmas (1). All RFLP profiles from the chicory samples were identical to STOL reference strain, indicating that diseased chicory was affected by a phytoplasma that belongs to ‘Candidatus Phytoplasma solani’ (16SrXII-A group). The 16S rDNA sequence of representative sample from symptomatic plant (Vp4) was deposited under accession number KF661322 in NCBI GenBank. It showed 100% identity to KF263684.1 from Iranian peach, JQ730742.1 from Serbian valerian, and JQ730750 from Serbian corn, all belonging to the ‘Ca. P. solani’ taxon. Puna chicory disease on C. intybus associated with a subgroup 16SrV-B of phytoplasma was detected in China (2). This is the first report of the Stolbur phytoplasma associated with fasciation of C. intybus in Serbia and worldwide.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Stolbur Phytoplasma Affecting Cichorium intybus in Serbia.",
pages = "840-839",
number = "6",
volume = "98",
doi = "10.1094/PDIS-09-13-0947-PDN"
}

Pavlović, S., Starović, M., Stojanović, S. D., Kojić, S., Marinković, J.,& Josić, D.. (2014). First Report of Stolbur Phytoplasma Affecting Cichorium intybus in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(6), 839-840.
https://doi.org/10.1094/PDIS-09-13-0947-PDN

Pavlović S, Starović M, Stojanović SD, Kojić S, Marinković J, Josić D. First Report of Stolbur Phytoplasma Affecting Cichorium intybus in Serbia.. in Plant Disease. 2014;98(6):839-840.
doi:10.1094/PDIS-09-13-0947-PDN .

Pavlović, S., Starović, M., Stojanović, S. D., Kojić, Snežana, Marinković, J., Josić, D., "First Report of Stolbur Phytoplasma Affecting Cichorium intybus in Serbia." in Plant Disease, 98, no. 6 (2014):839-840,
https://doi.org/10.1094/PDIS-09-13-0947-PDN . .

TY  - JOUR
AU  - Starović, M.
AU  - Kojić, Snežana
AU  - Kuzmanović, S. T.
AU  - Stojanović, S. D.
AU  - Pavlović, S.
AU  - Josić, D.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/695
AB  - Blueberries (Vaccinium corymbosum) are among the healthiest fruits due to their high antioxidant content. The total growing area of blueberries in Serbia ranges from 80 to 90 ha. A phytoplasma-like disease was observed for the first time during July 2009 in three blueberry cultivars (Bluecrop, Duke, and Spartan) grown in central Serbia, locality Kopljare (44°20′10.9″ N, 20°38′39.3″ E). Symptoms of yellowing and reddening were observed on the upper leaves and proliferating shoots, similar to those already described on blueberries (4). There was uneven ripening of the fruits on affected plants. Incidence of affected plants within a single field was estimated to be greater than 20% in 2009 and 50% in 2010. Blueberry leaves, together with petioles, were collected during two seasons, 2009 and 2010, and six samples from diseased plants and one from symptomless plants from each cultivar, resulting in 42 samples in total. For phytoplasma detection, total DNA was extracted from the veins of symptomatic and asymptomatic leaves of V. corymbosum using the protocol of Angelini et al. (1). Universal oligonucleotide primers P1/P7 were used to amplify a 1.8-kb DNA fragment containing the 16S rRNA gene, the 16S-23S spacer region, and the 5′ end of the 23S rRNA gene. Subsequently, a 1.2-kb fragment of the 16S rRNA gene was amplified by nested PCR with the R16F2n/R16R2 primers. Reactions were performed in a volume of 50 μl using Dream Taq Green master mix (Thermo Scientific, Lithuania). PCR reaction conditions were as reported (3), except for R16F2n/R2 primers set (annealing for 30 s at 58°C). PCR products were obtained only from the DNA of symptomatic plants. Fragments of 1.2 kb were further characterized by the PCR-RFLP analysis, using AluI, HpaII, HhaI, and Tru1I restriction enzymes (Thermo Scientific, Lithuania), as recommended by the manufacturer. The products of restriction enzyme digestion were separated by electrophoresis on 2.5% agarose gel. All R16F2n/R2 amplicons showed identical RFLP patterns corresponding to the profile of the Stolbur phytoplasma (subgroup 16SrXII-A). The results were confirmed by sequencing the nested PCR product from the representative strain Br1. The sequence was deposited in NCBI GenBank database under accession number KC960486. Phylogenetic analysis showed maximal similarities with SH1 isolate from Vitis vinifera, Jordan (KC835139.1), Bushehr (Iran) eggplant big bud phytoplasma (JX483703.1), BA strain isolated from insect in Italy (JQ868436.1), and also with several plants from Serbia: Arnica montana L. (JX891383.1), corn (JQ730750.1), Hypericum perforatum (JQ033928.1), tobacco (JQ730740.1), etc. In conclusion, our results demonstrate that leaf discoloration of V. corymbosum was associated with a phytoplasma belonging to the 16SrXII-A subgroup. The wild European blueberry (Vaccinium myrtillus L.) is already detected as a host plant of 16SrIII-F phytoplasma in Germany, North America, and Lithuania (4). The main vector of the Stolbur phytoplasma, Hyalesthes obsoletus Signoret, was already detected in Serbia (2). The first report of Stolbur phytoplasma occurrence on blueberry in Serbia is significant for the management of the pathogen spreading in blueberry fields. Since the cultivation of blueberry has a great economic potential in the region, it is important to identify emerging disease concerns in order to ensure sustainable production.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma
EP  - 1653
IS  - 12
SP  - 1653
VL  - 97
DO  - 10.1094/PDIS-05-13-0521-PDN
ER  - 

@article{
author = "Starović, M. and Kojić, Snežana and Kuzmanović, S. T. and Stojanović, S. D. and Pavlović, S. and Josić, D.",
year = "2013",
abstract = "Blueberries (Vaccinium corymbosum) are among the healthiest fruits due to their high antioxidant content. The total growing area of blueberries in Serbia ranges from 80 to 90 ha. A phytoplasma-like disease was observed for the first time during July 2009 in three blueberry cultivars (Bluecrop, Duke, and Spartan) grown in central Serbia, locality Kopljare (44°20′10.9″ N, 20°38′39.3″ E). Symptoms of yellowing and reddening were observed on the upper leaves and proliferating shoots, similar to those already described on blueberries (4). There was uneven ripening of the fruits on affected plants. Incidence of affected plants within a single field was estimated to be greater than 20% in 2009 and 50% in 2010. Blueberry leaves, together with petioles, were collected during two seasons, 2009 and 2010, and six samples from diseased plants and one from symptomless plants from each cultivar, resulting in 42 samples in total. For phytoplasma detection, total DNA was extracted from the veins of symptomatic and asymptomatic leaves of V. corymbosum using the protocol of Angelini et al. (1). Universal oligonucleotide primers P1/P7 were used to amplify a 1.8-kb DNA fragment containing the 16S rRNA gene, the 16S-23S spacer region, and the 5′ end of the 23S rRNA gene. Subsequently, a 1.2-kb fragment of the 16S rRNA gene was amplified by nested PCR with the R16F2n/R16R2 primers. Reactions were performed in a volume of 50 μl using Dream Taq Green master mix (Thermo Scientific, Lithuania). PCR reaction conditions were as reported (3), except for R16F2n/R2 primers set (annealing for 30 s at 58°C). PCR products were obtained only from the DNA of symptomatic plants. Fragments of 1.2 kb were further characterized by the PCR-RFLP analysis, using AluI, HpaII, HhaI, and Tru1I restriction enzymes (Thermo Scientific, Lithuania), as recommended by the manufacturer. The products of restriction enzyme digestion were separated by electrophoresis on 2.5% agarose gel. All R16F2n/R2 amplicons showed identical RFLP patterns corresponding to the profile of the Stolbur phytoplasma (subgroup 16SrXII-A). The results were confirmed by sequencing the nested PCR product from the representative strain Br1. The sequence was deposited in NCBI GenBank database under accession number KC960486. Phylogenetic analysis showed maximal similarities with SH1 isolate from Vitis vinifera, Jordan (KC835139.1), Bushehr (Iran) eggplant big bud phytoplasma (JX483703.1), BA strain isolated from insect in Italy (JQ868436.1), and also with several plants from Serbia: Arnica montana L. (JX891383.1), corn (JQ730750.1), Hypericum perforatum (JQ033928.1), tobacco (JQ730740.1), etc. In conclusion, our results demonstrate that leaf discoloration of V. corymbosum was associated with a phytoplasma belonging to the 16SrXII-A subgroup. The wild European blueberry (Vaccinium myrtillus L.) is already detected as a host plant of 16SrIII-F phytoplasma in Germany, North America, and Lithuania (4). The main vector of the Stolbur phytoplasma, Hyalesthes obsoletus Signoret, was already detected in Serbia (2). The first report of Stolbur phytoplasma occurrence on blueberry in Serbia is significant for the management of the pathogen spreading in blueberry fields. Since the cultivation of blueberry has a great economic potential in the region, it is important to identify emerging disease concerns in order to ensure sustainable production.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma",
pages = "1653-1653",
number = "12",
volume = "97",
doi = "10.1094/PDIS-05-13-0521-PDN"
}

Starović, M., Kojić, S., Kuzmanović, S. T., Stojanović, S. D., Pavlović, S.,& Josić, D.. (2013). First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(12), 1653-1653.
https://doi.org/10.1094/PDIS-05-13-0521-PDN

Starović M, Kojić S, Kuzmanović ST, Stojanović SD, Pavlović S, Josić D. First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma. in Plant Disease. 2013;97(12):1653-1653.
doi:10.1094/PDIS-05-13-0521-PDN .

Starović, M., Kojić, Snežana, Kuzmanović, S. T., Stojanović, S. D., Pavlović, S., Josić, D., "First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma" in Plant Disease, 97, no. 12 (2013):1653-1653,
https://doi.org/10.1094/PDIS-05-13-0521-PDN . .

TY  - JOUR
AU  - Đurić, S.
AU  - Pavić, Aleksandar
AU  - Jarak, M.
AU  - Pavlović, S.
AU  - Starović, M.
AU  - Pivić, R.
AU  - Josić, D.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/527
AB  - Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.
T2  - Romanian Biotechnological Letters
T1  - Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR
EP  - 6590
IS  - 5
SP  - 6580
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_imagine_527
ER  - 

@article{
author = "Đurić, S. and Pavić, Aleksandar and Jarak, M. and Pavlović, S. and Starović, M. and Pivić, R. and Josić, D.",
year = "2011",
abstract = "Among 268 bacterial isolates obtained from maize rhizospheric soil from Novi Sad, Vojvodina. Serbia. 59 were fluorescent pseudomonads. According to intrinsic antibiotic resistance (JAR) and heavy metal tolerance (HMT) patterns, the six representative isolates were selected and their diversity was assessed. Lytic enzyme activity (protease, chitinase, lipase, phospolipase, cellulase, gellatinase, pectinase, urease), plant-growth promoting treats (P-solubilization, siderophores, HCN and IAA production) and plant pathogenicity estimation (on lilac leaf apple and bean pods) resulted in selection of three isolates (PS2, P54, PS6) with the outlook for agriculture application. Antifungal activity was estimated on dual culture with eight phytopathogenic fungi (Curvularia lunata, Fusarium semitectum, Fusarium equiseti from Salvia officinalis L., F.equiseti from Matricaria chamomilla L.. Myrothecium verrucaria, Verticillium sp., Diaporte eres complex and Sclerotinia sclerotiorum) isolated from medicinal plants in Serbia. Isolate PS2 showed hyphal deformation of all investigated fungi and effective inhibition of mycelial growth of 7 out of 8 phytopatogenic fungi, partly due to production of chitinases, siderpohores and lytic enzymes. Abundant production of IAA (14 to 37 mM) and siderophores, phosphate solubilization and especially fungal growth inhibition make it suitable for further investigation, field trials and possible application in maize cultivation as biocontrol agent.",
journal = "Romanian Biotechnological Letters",
title = "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR",
pages = "6590-6580",
number = "5",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_imagine_527"
}

Đurić, S., Pavić, A., Jarak, M., Pavlović, S., Starović, M., Pivić, R.,& Josić, D.. (2011). Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters, 16(5), 6580-6590.
https://hdl.handle.net/21.15107/rcub_imagine_527

Đurić S, Pavić A, Jarak M, Pavlović S, Starović M, Pivić R, Josić D. Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR. in Romanian Biotechnological Letters. 2011;16(5):6580-6590.
https://hdl.handle.net/21.15107/rcub_imagine_527 .

Đurić, S., Pavić, Aleksandar, Jarak, M., Pavlović, S., Starović, M., Pivić, R., Josić, D., "Selection of indigenous fluorescent pseudomonad isolates from maize rhizospheric soil in Vojvodina as possible PGPR" in Romanian Biotechnological Letters, 16, no. 5 (2011):6580-6590,
https://hdl.handle.net/21.15107/rcub_imagine_527 .