TY  - JOUR
AU  - Drakul, Marija
AU  - Tomić, Sergej
AU  - Bekić, Marina
AU  - Mihajlović, Dušan
AU  - Vasiljević, Miloš
AU  - Rakočević, Sara
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Bokonjić, Dejan
AU  - Čolić, Miodrag
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/23/16829
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2220
AB  - Sitagliptin, an anti-diabetic drug, is a dipeptidyl peptidase (DPP)-4/CD26 inhibitor with additional anti-inflammatory and immunomodulatory properties. In this study, we investigated for the first time the effect of sitagliptin on the differentiation and functions of human dendritic cells generated from monocytes (MoDCs) for 4 days using the standard GM-CSF/IL-4 procedure. LPS/IFN-γ treatment for an additional 24 h was used for maturation induction of MoDCs. Sitagliptin was added at the highest non-cytotoxic concentration (500 µg/mL) either at the beginning (sita 0d protocol) or after MoDC differentiation (sita 4d protocol). Sitagliptin impaired differentiation and maturation of MoDCs as judged with the lower expression of CD40, CD83, CD86, NLRP3, and HLA-DR, retention of CD14 expression, and inhibited production of IL-β, IL-12p70, IL-23, and IL-27. In contrast, the expression of CD26, tolerogenic DC markers (ILT4 and IDO1), and production of immunoregulatory cytokines (IL-10 and TGF-β) were increased. Generally, the sita 0d protocol was more efficient. Sitagliptin-treated MoDCs were poorer allostimulators of T-cells in MoDC/T-cell co-culture and inhibited Th1 and Th17 but augmented Th2 and Treg responses. Tolerogenic properties of sitagliptin-treated MoDCs were additionally confirmed by an increased frequency of CD4+CD25+CD127- FoxP3+ Tregs and Tr1 cells (CD4+IL-10+FoxP3-) in MoDC/T-cell co-culture. The differentiation of IL-10+ and TGF-β+ Tregs depended on the sitagliptin protocol used. A Western blot analysis showed that sitagliptin inhibited p65 expression of NF-kB and p38MAPK during the maturation of MoDCs. In conclusion, sitagliptin induces differentiation of tolerogenic DCs, and the effect is important when considering sitagliptin for treating autoimmune diseases and allotransplant rejection.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Sitagliptin Induces Tolerogenic Human Dendritic Cells
IS  - 23
SP  - 16829
VL  - 24
DO  - 10.3390/ijms242316829
ER  - 

@article{
author = "Drakul, Marija and Tomić, Sergej and Bekić, Marina and Mihajlović, Dušan and Vasiljević, Miloš and Rakočević, Sara and Đokić, Jelena and Popović, Nikola and Bokonjić, Dejan and Čolić, Miodrag",
year = "2023",
abstract = "Sitagliptin, an anti-diabetic drug, is a dipeptidyl peptidase (DPP)-4/CD26 inhibitor with additional anti-inflammatory and immunomodulatory properties. In this study, we investigated for the first time the effect of sitagliptin on the differentiation and functions of human dendritic cells generated from monocytes (MoDCs) for 4 days using the standard GM-CSF/IL-4 procedure. LPS/IFN-γ treatment for an additional 24 h was used for maturation induction of MoDCs. Sitagliptin was added at the highest non-cytotoxic concentration (500 µg/mL) either at the beginning (sita 0d protocol) or after MoDC differentiation (sita 4d protocol). Sitagliptin impaired differentiation and maturation of MoDCs as judged with the lower expression of CD40, CD83, CD86, NLRP3, and HLA-DR, retention of CD14 expression, and inhibited production of IL-β, IL-12p70, IL-23, and IL-27. In contrast, the expression of CD26, tolerogenic DC markers (ILT4 and IDO1), and production of immunoregulatory cytokines (IL-10 and TGF-β) were increased. Generally, the sita 0d protocol was more efficient. Sitagliptin-treated MoDCs were poorer allostimulators of T-cells in MoDC/T-cell co-culture and inhibited Th1 and Th17 but augmented Th2 and Treg responses. Tolerogenic properties of sitagliptin-treated MoDCs were additionally confirmed by an increased frequency of CD4+CD25+CD127- FoxP3+ Tregs and Tr1 cells (CD4+IL-10+FoxP3-) in MoDC/T-cell co-culture. The differentiation of IL-10+ and TGF-β+ Tregs depended on the sitagliptin protocol used. A Western blot analysis showed that sitagliptin inhibited p65 expression of NF-kB and p38MAPK during the maturation of MoDCs. In conclusion, sitagliptin induces differentiation of tolerogenic DCs, and the effect is important when considering sitagliptin for treating autoimmune diseases and allotransplant rejection.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Sitagliptin Induces Tolerogenic Human Dendritic Cells",
number = "23",
pages = "16829",
volume = "24",
doi = "10.3390/ijms242316829"
}

Drakul, M., Tomić, S., Bekić, M., Mihajlović, D., Vasiljević, M., Rakočević, S., Đokić, J., Popović, N., Bokonjić, D.,& Čolić, M.. (2023). Sitagliptin Induces Tolerogenic Human Dendritic Cells. in International Journal of Molecular Sciences
MDPI., 24(23), 16829.
https://doi.org/10.3390/ijms242316829

Drakul M, Tomić S, Bekić M, Mihajlović D, Vasiljević M, Rakočević S, Đokić J, Popović N, Bokonjić D, Čolić M. Sitagliptin Induces Tolerogenic Human Dendritic Cells. in International Journal of Molecular Sciences. 2023;24(23):16829.
doi:10.3390/ijms242316829 .

Drakul, Marija, Tomić, Sergej, Bekić, Marina, Mihajlović, Dušan, Vasiljević, Miloš, Rakočević, Sara, Đokić, Jelena, Popović, Nikola, Bokonjić, Dejan, Čolić, Miodrag, "Sitagliptin Induces Tolerogenic Human Dendritic Cells" in International Journal of Molecular Sciences, 24, no. 23 (2023):16829,
https://doi.org/10.3390/ijms242316829 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Brdarić, Emilija
AU  - Bajić, Svetlana Soković
AU  - Đokić, Jelena
AU  - Živković, Milica
AU  - Veljović, Katarina
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/47047
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2296
AB  - One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains
T1  - Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana
EP  - 570
IS  - Notebook 6
SP  - 554
VL  - 73
DO  - 10.5937/arhfarm73-47047
ER  - 

@article{
author = "Popović, Nikola and Terzić-Vidojević, Amarela and Brdarić, Emilija and Bajić, Svetlana Soković and Đokić, Jelena and Živković, Milica and Veljović, Katarina",
year = "2023",
abstract = "One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains, Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana",
pages = "570-554",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-47047"
}

Popović, N., Terzić-Vidojević, A., Brdarić, E., Bajić, S. S., Đokić, J., Živković, M.,& Veljović, K.. (2023). Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 554-570.
https://doi.org/10.5937/arhfarm73-47047

Popović N, Terzić-Vidojević A, Brdarić E, Bajić SS, Đokić J, Živković M, Veljović K. Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy. 2023;73(Notebook 6):554-570.
doi:10.5937/arhfarm73-47047 .

Popović, Nikola, Terzić-Vidojević, Amarela, Brdarić, Emilija, Bajić, Svetlana Soković, Đokić, Jelena, Živković, Milica, Veljović, Katarina, "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains" in Archives of Pharmacy, 73, no. Notebook 6 (2023):554-570,
https://doi.org/10.5937/arhfarm73-47047 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Stevanović, Dušan
AU  - Radojević, Dušan
AU  - Veljović, Katarina
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2023
UR  - https://www.mdpi.com/2076-2607/11/12/2844
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2209
AB  - This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing Enterococcus faecium BGZLM1-5 and its potential application in reducing Listeria monocytogenes in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (v/v) of the bacteriocin-containing supernatant and 0.5% (v/v) of the partially purified bacteriocin decreased the viable number of L. monocytogenes ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.
T2  - Microorganisms
T1  - Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model
IS  - 12
SP  - 2844
VL  - 11
DO  - 10.3390/microorganisms11122844
ER  - 

@article{
author = "Popović, Nikola and Stevanović, Dušan and Radojević, Dušan and Veljović, Katarina and Đokić, Jelena and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2023",
abstract = "This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing Enterococcus faecium BGZLM1-5 and its potential application in reducing Listeria monocytogenes in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (v/v) of the bacteriocin-containing supernatant and 0.5% (v/v) of the partially purified bacteriocin decreased the viable number of L. monocytogenes ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.",
journal = "Microorganisms",
title = "Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model",
number = "12",
pages = "2844",
volume = "11",
doi = "10.3390/microorganisms11122844"
}

Popović, N., Stevanović, D., Radojević, D., Veljović, K., Đokić, J., Golić, N.,& Terzić-Vidojević, A.. (2023). Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model. in Microorganisms, 11(12), 2844.
https://doi.org/10.3390/microorganisms11122844

Popović N, Stevanović D, Radojević D, Veljović K, Đokić J, Golić N, Terzić-Vidojević A. Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model. in Microorganisms. 2023;11(12):2844.
doi:10.3390/microorganisms11122844 .

Popović, Nikola, Stevanović, Dušan, Radojević, Dušan, Veljović, Katarina, Đokić, Jelena, Golić, Nataša, Terzić-Vidojević, Amarela, "Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model" in Microorganisms, 11, no. 12 (2023):2844,
https://doi.org/10.3390/microorganisms11122844 . .

TY  - JOUR
AU  - Tomić, Jelena
AU  - Dapčević-Hadnađev, Tamara
AU  - Škrobot, Dubravka
AU  - Maravić, Nikola
AU  - Popović, Nikola
AU  - Stevanović, Dušan
AU  - Hadnađev, Miroslav
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0023643823000609
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2241
AB  - In this study changes during fermentation of spontaneously fermented modern and ancient (spelt, emmer, and khorasan) wheat sourdoughs and their feasibility to act as leavening agents in breadmaking were evaluated. During 6 h of fermentation, sourdough was characterized for lactic acid bacteria and yeasts counts, pH, total titratable acidity (TTA), proteolytic activity, rheological and electrophoretic profiles. The effects of 25 and 50 g/100g sourdough addition on physico-chemical and sensory properties of refined wheat flour bread were also investigated. Although in all sourdoughs a pH drop, increase in proteolytic activity, gliadin and glutenin degradation with fermentation were recorded, due to different flour composition and microbial activity, tested sourdoughs significantly differed in rheological properties and breadmaking quality. Wheat sourdough being characterized by the highest acidification, increase in proteolytic activity, and the most extensive hydrolysis of gliadins, resulted in bread with the lowest specific volume and hardest crumb texture. Emmer sourdough, having the highest TTA, ash and wet gluten content, exhibited the highest extensibility, the least pronounced changes in proteolytic activity and the electrophoretic pattern which produced bread with the highest volume and softest texture. In general, ancient wheat varieties have shown great potential in sourdough breadmaking in comparison to modern wheat.
T2  - LWT
T1  - Spontaneously fermented ancient wheat sourdoughs in breadmaking: Impact of flour quality on sourdough and bread physico-chemical properties
SP  - 114482
VL  - 175
DO  - 10.1016/j.lwt.2023.114482
ER  - 

@article{
author = "Tomić, Jelena and Dapčević-Hadnađev, Tamara and Škrobot, Dubravka and Maravić, Nikola and Popović, Nikola and Stevanović, Dušan and Hadnađev, Miroslav",
year = "2023",
abstract = "In this study changes during fermentation of spontaneously fermented modern and ancient (spelt, emmer, and khorasan) wheat sourdoughs and their feasibility to act as leavening agents in breadmaking were evaluated. During 6 h of fermentation, sourdough was characterized for lactic acid bacteria and yeasts counts, pH, total titratable acidity (TTA), proteolytic activity, rheological and electrophoretic profiles. The effects of 25 and 50 g/100g sourdough addition on physico-chemical and sensory properties of refined wheat flour bread were also investigated. Although in all sourdoughs a pH drop, increase in proteolytic activity, gliadin and glutenin degradation with fermentation were recorded, due to different flour composition and microbial activity, tested sourdoughs significantly differed in rheological properties and breadmaking quality. Wheat sourdough being characterized by the highest acidification, increase in proteolytic activity, and the most extensive hydrolysis of gliadins, resulted in bread with the lowest specific volume and hardest crumb texture. Emmer sourdough, having the highest TTA, ash and wet gluten content, exhibited the highest extensibility, the least pronounced changes in proteolytic activity and the electrophoretic pattern which produced bread with the highest volume and softest texture. In general, ancient wheat varieties have shown great potential in sourdough breadmaking in comparison to modern wheat.",
journal = "LWT",
title = "Spontaneously fermented ancient wheat sourdoughs in breadmaking: Impact of flour quality on sourdough and bread physico-chemical properties",
pages = "114482",
volume = "175",
doi = "10.1016/j.lwt.2023.114482"
}

Tomić, J., Dapčević-Hadnađev, T., Škrobot, D., Maravić, N., Popović, N., Stevanović, D.,& Hadnađev, M.. (2023). Spontaneously fermented ancient wheat sourdoughs in breadmaking: Impact of flour quality on sourdough and bread physico-chemical properties. in LWT, 175, 114482.
https://doi.org/10.1016/j.lwt.2023.114482

Tomić J, Dapčević-Hadnađev T, Škrobot D, Maravić N, Popović N, Stevanović D, Hadnađev M. Spontaneously fermented ancient wheat sourdoughs in breadmaking: Impact of flour quality on sourdough and bread physico-chemical properties. in LWT. 2023;175:114482.
doi:10.1016/j.lwt.2023.114482 .

Tomić, Jelena, Dapčević-Hadnađev, Tamara, Škrobot, Dubravka, Maravić, Nikola, Popović, Nikola, Stevanović, Dušan, Hadnađev, Miroslav, "Spontaneously fermented ancient wheat sourdoughs in breadmaking: Impact of flour quality on sourdough and bread physico-chemical properties" in LWT, 175 (2023):114482,
https://doi.org/10.1016/j.lwt.2023.114482 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2091
AB  - Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.
PB  - Elsevier
PB  - American Dairy Science Association
T2  - Journal of Dairy Science
T2  - Journal of Dairy ScienceJournal of Dairy Science
T1  - Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans
EP  - 7460
IS  - 11
SP  - 7447
VL  - 106
DO  - 10.3168/jds.2023-23342
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Popović, Nikola and Radojević, Dušan and Veljović, Katarina and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2023",
abstract = "Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.",
publisher = "Elsevier, American Dairy Science Association",
journal = "Journal of Dairy Science, Journal of Dairy ScienceJournal of Dairy Science",
title = "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans",
pages = "7460-7447",
number = "11",
volume = "106",
doi = "10.3168/jds.2023-23342"
}

Dinić, M., Jakovljević, S., Popović, N., Radojević, D., Veljović, K., Golić, N.,& Terzić-Vidojević, A.. (2023). Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science
Elsevier., 106(11), 7447-7460.
https://doi.org/10.3168/jds.2023-23342

Dinić M, Jakovljević S, Popović N, Radojević D, Veljović K, Golić N, Terzić-Vidojević A. Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science. 2023;106(11):7447-7460.
doi:10.3168/jds.2023-23342 .

Dinić, Miroslav, Jakovljević, Stefan, Popović, Nikola, Radojević, Dušan, Veljović, Katarina, Golić, Nataša, Terzić-Vidojević, Amarela, "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans" in Journal of Dairy Science, 106, no. 11 (2023):7447-7460,
https://doi.org/10.3168/jds.2023-23342 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Đokić, Jelena
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46614
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2297
AB  - Probiotic lactobacilli exhibit the potential to promote health benefits for the host. Thanks to its numerous beneficial effects on human health, Limosilactobacillus fermentum stood out as an excellent candidate for the development of commercial probiotic preparations aiming to prevent community-acquired infections. In this study, several in vitro tests, including biofilm formation assay, assessment of antibiotic susceptibility, survival in simulated gastrointestinal tract conditions and attachment to intestinal Caco-2 cells, were used to estimate the safety and probiotic potential of L. fermentum BGHV110 strain. Additionally, Caenorhabditis elegans was used as an in vivo model system for the evaluation of L. fermentum BGHV110 influence on the host’s innate immune response. The results revealed that L. fermentum BGHV110 strain showed an excellent capability to survive harsh conditions of the gut, to attach to intestinal Caco-2 cells and to stimulate conserved p38 MAPK immunity pathway and expression of the clc-1 claudin-like gene and antimicrobial peptides in C. elegans in order to enhance the immune response against pathogens. Finally, L. fermentum BGHV110 showed no virulence traits and susceptibility to tested antibiotics, confirming its safety status which enables it to be applied as a future probiotic.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans
T1  - Probiotička karakterizacija soja Limosilactobacillus fermentum BGHV110 i njegov uticaj na urođeni imunski odgovor kod Caenorhabditis elegans
EP  - 585
IS  - Notebook 6
SP  - 571
VL  - 73
DO  - 10.5937/arhfarm73-46614
ER  - 

@article{
author = "Dinić, Miroslav and Popović, Nikola and Radojević, Dušan and Đokić, Jelena",
year = "2023",
abstract = "Probiotic lactobacilli exhibit the potential to promote health benefits for the host. Thanks to its numerous beneficial effects on human health, Limosilactobacillus fermentum stood out as an excellent candidate for the development of commercial probiotic preparations aiming to prevent community-acquired infections. In this study, several in vitro tests, including biofilm formation assay, assessment of antibiotic susceptibility, survival in simulated gastrointestinal tract conditions and attachment to intestinal Caco-2 cells, were used to estimate the safety and probiotic potential of L. fermentum BGHV110 strain. Additionally, Caenorhabditis elegans was used as an in vivo model system for the evaluation of L. fermentum BGHV110 influence on the host’s innate immune response. The results revealed that L. fermentum BGHV110 strain showed an excellent capability to survive harsh conditions of the gut, to attach to intestinal Caco-2 cells and to stimulate conserved p38 MAPK immunity pathway and expression of the clc-1 claudin-like gene and antimicrobial peptides in C. elegans in order to enhance the immune response against pathogens. Finally, L. fermentum BGHV110 showed no virulence traits and susceptibility to tested antibiotics, confirming its safety status which enables it to be applied as a future probiotic.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans, Probiotička karakterizacija soja Limosilactobacillus fermentum BGHV110 i njegov uticaj na urođeni imunski odgovor kod Caenorhabditis elegans",
pages = "585-571",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-46614"
}

Dinić, M., Popović, N., Radojević, D.,& Đokić, J.. (2023). Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 571-585.
https://doi.org/10.5937/arhfarm73-46614

Dinić M, Popović N, Radojević D, Đokić J. Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans. in Archives of Pharmacy. 2023;73(Notebook 6):571-585.
doi:10.5937/arhfarm73-46614 .

Dinić, Miroslav, Popović, Nikola, Radojević, Dušan, Đokić, Jelena, "Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans" in Archives of Pharmacy, 73, no. Notebook 6 (2023):571-585,
https://doi.org/10.5937/arhfarm73-46614 . .

TY  - JOUR
AU  - Babić, Mirjana
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Golić, Nataša
AU  - Radojković, Dragica
AU  - Stanković, Marija
PY  - 2023
UR  - https://doi.org/10.1093/jambio/lxad257
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2204
AB  - Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells.The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke stimulated BEAS-2B cells and 3-D bronchospheres organoids. We showed that HK Lactiplantibacillus plantarum BGPKM22 possesses antioxidant activity against cigarette smoke, resistance to hydrogen peroxide, and free radical neutralizing activity. We demonstrated that HK BGPKM22 inhibited cigarette smoke induced expression of the Aryl hydrocarbon receptor (AhR) and Nuclear factor erythroid 2 related factor 2 (Nrf2) genes. The cell-free supernatant (SN) of BGPKM22 fully confirmed the effects of HK BGPKM22.For the first time, we revealed that HK and SN of L. plantarum BGPKM22 possess antioxidant activity and modulate AhR and Nrf2 gene expression in bronchial epithelial cells exposed to cigarette smoke.
T2  - Journal of Applied Microbiology
T1  - Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke
SP  - lxad257
VL  - n/a
DO  - 10.1093/jambio/lxad257
ER  - 

@article{
author = "Babić, Mirjana and Veljović, Katarina and Popović, Nikola and Golić, Nataša and Radojković, Dragica and Stanković, Marija",
year = "2023",
abstract = "Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells.The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke stimulated BEAS-2B cells and 3-D bronchospheres organoids. We showed that HK Lactiplantibacillus plantarum BGPKM22 possesses antioxidant activity against cigarette smoke, resistance to hydrogen peroxide, and free radical neutralizing activity. We demonstrated that HK BGPKM22 inhibited cigarette smoke induced expression of the Aryl hydrocarbon receptor (AhR) and Nuclear factor erythroid 2 related factor 2 (Nrf2) genes. The cell-free supernatant (SN) of BGPKM22 fully confirmed the effects of HK BGPKM22.For the first time, we revealed that HK and SN of L. plantarum BGPKM22 possess antioxidant activity and modulate AhR and Nrf2 gene expression in bronchial epithelial cells exposed to cigarette smoke.",
journal = "Journal of Applied Microbiology",
title = "Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke",
pages = "lxad257",
volume = "n/a",
doi = "10.1093/jambio/lxad257"
}

Babić, M., Veljović, K., Popović, N., Golić, N., Radojković, D.,& Stanković, M.. (2023). Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke. in Journal of Applied Microbiology, n/a, lxad257.
https://doi.org/10.1093/jambio/lxad257

Babić M, Veljović K, Popović N, Golić N, Radojković D, Stanković M. Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke. in Journal of Applied Microbiology. 2023;n/a:lxad257.
doi:10.1093/jambio/lxad257 .

Babić, Mirjana, Veljović, Katarina, Popović, Nikola, Golić, Nataša, Radojković, Dragica, Stanković, Marija, "Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke" in Journal of Applied Microbiology, n/a (2023):lxad257,
https://doi.org/10.1093/jambio/lxad257 . .

TY  - JOUR
AU  - Škrobot, Dubravka
AU  - Dapčević-Hadnađev, Tamara
AU  - Tomić, Jelena
AU  - Maravić, Nikola
AU  - Popović, Nikola
AU  - Jovanov, Pavle
AU  - Hadnađev, Miroslav
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1666
AB  - The aim of this study was to test the suitability of three different ancient wheat varieties (emmer, spelt and khorasan) to produce spontaneously fermented sourdough bread and to evaluate the impact on the dough rheological properties, ultrastructure and baking quality. Modern wheat sourdough bread and bakery yeast fermented bread were used as controls. Sourdoughs produced from modern and ancient wheats exerted different effects on dough viscoelastic properties, bread specific volume, texture, firming rate, colour and sensory properties, while there was no influence on bread water activity. Both khorasan sourdough, being characterised with the highest dough strength and dense gluten protein matrix, and emmer sourdough, with loose and thin gluten strands of low strength, yielded breads characterised by low specific volume and hard crumb texture. Spelt and modern wheat sourdough were characterised by foam-like dough structures with entrapped gas cells leading to breads of similar specific volume and texture. Although the yeast-fermented wheat flour exerted a higher specific volume and the lowest firmness, the sourdough wheat flour bread had a lower firming rate. A comparison of sourdough bread prepared with modern and ancient wheats revealed that breads based on ancient varieties possess a less noticeable sour taste, odour and flavour, thus contributing to more sensory-appealing sourdough bread.
T2  - Foods
T2  - Foods
T1  - Techno-Functional Performance of Emmer, Spelt and Khorasan in Spontaneously Fermented Sourdough Bread
IS  - 23
SP  - 3927
VL  - 11
DO  - 10.3390/foods11233927
ER  - 

@article{
author = "Škrobot, Dubravka and Dapčević-Hadnađev, Tamara and Tomić, Jelena and Maravić, Nikola and Popović, Nikola and Jovanov, Pavle and Hadnađev, Miroslav",
year = "2022",
abstract = "The aim of this study was to test the suitability of three different ancient wheat varieties (emmer, spelt and khorasan) to produce spontaneously fermented sourdough bread and to evaluate the impact on the dough rheological properties, ultrastructure and baking quality. Modern wheat sourdough bread and bakery yeast fermented bread were used as controls. Sourdoughs produced from modern and ancient wheats exerted different effects on dough viscoelastic properties, bread specific volume, texture, firming rate, colour and sensory properties, while there was no influence on bread water activity. Both khorasan sourdough, being characterised with the highest dough strength and dense gluten protein matrix, and emmer sourdough, with loose and thin gluten strands of low strength, yielded breads characterised by low specific volume and hard crumb texture. Spelt and modern wheat sourdough were characterised by foam-like dough structures with entrapped gas cells leading to breads of similar specific volume and texture. Although the yeast-fermented wheat flour exerted a higher specific volume and the lowest firmness, the sourdough wheat flour bread had a lower firming rate. A comparison of sourdough bread prepared with modern and ancient wheats revealed that breads based on ancient varieties possess a less noticeable sour taste, odour and flavour, thus contributing to more sensory-appealing sourdough bread.",
journal = "Foods, Foods",
title = "Techno-Functional Performance of Emmer, Spelt and Khorasan in Spontaneously Fermented Sourdough Bread",
number = "23",
pages = "3927",
volume = "11",
doi = "10.3390/foods11233927"
}

Škrobot, D., Dapčević-Hadnađev, T., Tomić, J., Maravić, N., Popović, N., Jovanov, P.,& Hadnađev, M.. (2022). Techno-Functional Performance of Emmer, Spelt and Khorasan in Spontaneously Fermented Sourdough Bread. in Foods, 11(23), 3927.
https://doi.org/10.3390/foods11233927

Škrobot D, Dapčević-Hadnađev T, Tomić J, Maravić N, Popović N, Jovanov P, Hadnađev M. Techno-Functional Performance of Emmer, Spelt and Khorasan in Spontaneously Fermented Sourdough Bread. in Foods. 2022;11(23):3927.
doi:10.3390/foods11233927 .

Škrobot, Dubravka, Dapčević-Hadnađev, Tamara, Tomić, Jelena, Maravić, Nikola, Popović, Nikola, Jovanov, Pavle, Hadnađev, Miroslav, "Techno-Functional Performance of Emmer, Spelt and Khorasan in Spontaneously Fermented Sourdough Bread" in Foods, 11, no. 23 (2022):3927,
https://doi.org/10.3390/foods11233927 . .

TY  - JOUR
AU  - Stanković, Marija
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Kojić, Snežana
AU  - Dunjić Manevski, Sofija
AU  - Radojković, Dragica
AU  - Golić, Nataša
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1538
AB  - Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells
IS  - 10
VL  - 23
DO  - 10.3390/ijms23105547
ER  - 

@article{
author = "Stanković, Marija and Veljović, Katarina and Popović, Nikola and Kojić, Snežana and Dunjić Manevski, Sofija and Radojković, Dragica and Golić, Nataša",
year = "2022",
abstract = "Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells",
number = "10",
volume = "23",
doi = "10.3390/ijms23105547"
}

Stanković, M., Veljović, K., Popović, N., Kojić, S., Dunjić Manevski, S., Radojković, D.,& Golić, N.. (2022). Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences
MDPI, Basel., 23(10).
https://doi.org/10.3390/ijms23105547

Stanković M, Veljović K, Popović N, Kojić S, Dunjić Manevski S, Radojković D, Golić N. Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences. 2022;23(10).
doi:10.3390/ijms23105547 .

Stanković, Marija, Veljović, Katarina, Popović, Nikola, Kojić, Snežana, Dunjić Manevski, Sofija, Radojković, Dragica, Golić, Nataša, "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells" in International Journal of Molecular Sciences, 23, no. 10 (2022),
https://doi.org/10.3390/ijms23105547 . .

TY  - JOUR
AU  - Karabegović, Ivana
AU  - Malicanin, Marko
AU  - Popović, Nikola
AU  - Stamenkovic Stojanović, Sandra
AU  - Lazić, Miodrag
AU  - Stanojević, Jelena
AU  - Danilović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1585
AB  - The enological potential of two previously characterized indigenous yeast isolates, Hanseniaspora uvarum S-2 and Candida famata WB-1, in pure and sequential inoculation with commercial yeast Saccharomyces cerevisiae QA23 were analyzed in industrial-scale vinification of the grape variety Tamjanika. Their contribution to the quality and aroma profile was investigated by quantifying volatile compounds and wine sensory evaluation. Both yeast isolates were able to complete alcoholic fermentation, to reduce ethanol concentration up to 1.06% v/v (in monoculture) in comparation to S. cerevisiae QA23, and to enhance aroma and sensory profile. Based on calculated odor activity values (OAV), p-cymene, ethyl hexanoate, ethyl octanoate, and ethyl decanoate were the major aroma volatile compounds in all Tamjanika wine samples. Analyzed yeast strains significantly affected relative contribution of volatile compounds and can be considered responsible for the differences and uniqueness of the obtained wine samples. Besides confirmation of good enological and fermentative characteristics, selected isolates can be characterized as high ester-producing strains with potential to enhance the floral and fruity aromas of wine. The present study represents a further step toward the use of indigenous yeast isolates at industrial-scale fermentation in order to ensure the regional signature of Tamjanika wine.
PB  - MDPI, Basel
T2  - Foods
T1  - Native Non-Saccharomyces Yeasts as a Tool to Produce Distinctive and Diverse Tamjanika Grape Wines
IS  - 13
VL  - 11
DO  - 10.3390/foods11131935
ER  - 

@article{
author = "Karabegović, Ivana and Malicanin, Marko and Popović, Nikola and Stamenkovic Stojanović, Sandra and Lazić, Miodrag and Stanojević, Jelena and Danilović, Bojana",
year = "2022",
abstract = "The enological potential of two previously characterized indigenous yeast isolates, Hanseniaspora uvarum S-2 and Candida famata WB-1, in pure and sequential inoculation with commercial yeast Saccharomyces cerevisiae QA23 were analyzed in industrial-scale vinification of the grape variety Tamjanika. Their contribution to the quality and aroma profile was investigated by quantifying volatile compounds and wine sensory evaluation. Both yeast isolates were able to complete alcoholic fermentation, to reduce ethanol concentration up to 1.06% v/v (in monoculture) in comparation to S. cerevisiae QA23, and to enhance aroma and sensory profile. Based on calculated odor activity values (OAV), p-cymene, ethyl hexanoate, ethyl octanoate, and ethyl decanoate were the major aroma volatile compounds in all Tamjanika wine samples. Analyzed yeast strains significantly affected relative contribution of volatile compounds and can be considered responsible for the differences and uniqueness of the obtained wine samples. Besides confirmation of good enological and fermentative characteristics, selected isolates can be characterized as high ester-producing strains with potential to enhance the floral and fruity aromas of wine. The present study represents a further step toward the use of indigenous yeast isolates at industrial-scale fermentation in order to ensure the regional signature of Tamjanika wine.",
publisher = "MDPI, Basel",
journal = "Foods",
title = "Native Non-Saccharomyces Yeasts as a Tool to Produce Distinctive and Diverse Tamjanika Grape Wines",
number = "13",
volume = "11",
doi = "10.3390/foods11131935"
}

Karabegović, I., Malicanin, M., Popović, N., Stamenkovic Stojanović, S., Lazić, M., Stanojević, J.,& Danilović, B.. (2022). Native Non-Saccharomyces Yeasts as a Tool to Produce Distinctive and Diverse Tamjanika Grape Wines. in Foods
MDPI, Basel., 11(13).
https://doi.org/10.3390/foods11131935

Karabegović I, Malicanin M, Popović N, Stamenkovic Stojanović S, Lazić M, Stanojević J, Danilović B. Native Non-Saccharomyces Yeasts as a Tool to Produce Distinctive and Diverse Tamjanika Grape Wines. in Foods. 2022;11(13).
doi:10.3390/foods11131935 .

Karabegović, Ivana, Malicanin, Marko, Popović, Nikola, Stamenkovic Stojanović, Sandra, Lazić, Miodrag, Stanojević, Jelena, Danilović, Bojana, "Native Non-Saccharomyces Yeasts as a Tool to Produce Distinctive and Diverse Tamjanika Grape Wines" in Foods, 11, no. 13 (2022),
https://doi.org/10.3390/foods11131935 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1464
AB  - The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.
PB  - MDPI, Basel
T2  - Foods
T1  - Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns
IS  - 11
VL  - 10
DO  - 10.3390/foods10112753
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Popović, Nikola and Tolinački, Maja and Golić, Nataša",
year = "2021",
abstract = "The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.",
publisher = "MDPI, Basel",
journal = "Foods",
title = "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns",
number = "11",
volume = "10",
doi = "10.3390/foods10112753"
}

Terzić-Vidojević, A., Veljović, K., Popović, N., Tolinački, M.,& Golić, N.. (2021). Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods
MDPI, Basel., 10(11).
https://doi.org/10.3390/foods10112753

Terzić-Vidojević A, Veljović K, Popović N, Tolinački M, Golić N. Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods. 2021;10(11).
doi:10.3390/foods10112753 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Popović, Nikola, Tolinački, Maja, Golić, Nataša, "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns" in Foods, 10, no. 11 (2021),
https://doi.org/10.3390/foods10112753 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Strahinić, Ivana
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1473
AB  - The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.
PB  - Nature Portfolio, Berlin
T2  - Scientific Reports
T1  - Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-00698-5
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Đokić, Jelena and Popović, Nikola and Radojević, Dušan and Strahinić, Ivana and Golić, Nataša",
year = "2021",
abstract = "The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.",
publisher = "Nature Portfolio, Berlin",
journal = "Scientific Reports",
title = "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-00698-5"
}

Dinić, M., Jakovljević, S., Đokić, J., Popović, N., Radojević, D., Strahinić, I.,& Golić, N.. (2021). Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports
Nature Portfolio, Berlin., 11(1).
https://doi.org/10.1038/s41598-021-00698-5

Dinić M, Jakovljević S, Đokić J, Popović N, Radojević D, Strahinić I, Golić N. Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-00698-5 .

Dinić, Miroslav, Jakovljević, Stefan, Đokić, Jelena, Popović, Nikola, Radojević, Dušan, Strahinić, Ivana, Golić, Nataša, "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-00698-5 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beskoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1337
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beskoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beskoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević N, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beskoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja, Soković Bajić, Svetlana, Jovanović, Živko, Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beskoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .

TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Tomić, Sergej
AU  - Popović, Nikola
AU  - Brdarić, Emilija
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1318
AB  - The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro
SP  - 1347
VL  - 10
DO  - 10.1038/s41598-020-58177-2
ER  - 

@article{
author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Tomić, Sergej and Popović, Nikola and Brdarić, Emilija and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro",
pages = "1347",
volume = "10",
doi = "10.1038/s41598-020-58177-2"
}

Soković Bajić, S., Đokić, J., Dinić, M., Tomić, S., Popović, N., Brdarić, E., Golić, N.,& Tolinački, M.. (2020). GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports
Nature Publishing Group, London., 10, 1347.
https://doi.org/10.1038/s41598-020-58177-2

Soković Bajić S, Đokić J, Dinić M, Tomić S, Popović N, Brdarić E, Golić N, Tolinački M. GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports. 2020;10:1347.
doi:10.1038/s41598-020-58177-2 .

Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Tomić, Sergej, Popović, Nikola, Brdarić, Emilija, Golić, Nataša, Tolinački, Maja, "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro" in Scientific Reports, 10 (2020):1347,
https://doi.org/10.1038/s41598-020-58177-2 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1344
AB  - Yogurt is a traditional fermented dairy product, prepared with starter cultures containing Streptococcus thermophilus and Lactobacillus bulgaricus that has gained widespread consumer acceptance as a healthy food. It is widely accepted that yogurt cultures have been recognized as probiotics, due to their beneficial effects on human health. In this study, we have characterized technological and health-promoting properties of autochthonous strains S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 isolated from artisanal sour milk and yogurt, respectively, in order to be used as functional yogurt starter cultures. Both BGKMJ1-36 and BGVLJ1-21 strains have the ability to form curd after five hours at 42 degrees C, hydrolyze alpha(s1)-, beta-, and kappa- casein, and to show antimicrobial activity toward Listeria monocytogenes. The strain BGKMJ1-36 produces exopolysaccharides important for rheological properties of the yogurt. The colonies of BGKMJ1-36 and BGVLJ1-21 strains that successfully survived transit of the yogurt through simulated gastrointestinal tract conditions have been tested for adhesion to intestinal epithelial Caco-2 cells. The results reveal that both strains adhere to Caco-2 cells and significantly upregulate the expression of autophagy-, tight junction proteins-, and anti-microbial peptides-related genes. Hence, both strains may be interesting for use as a novel functional starter culture for production of added-value yogurt with health-promoting properties.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro
IS  - 10
VL  - 8
DO  - 10.3390/microorganisms8101586
ER  - 

@article{
author = "Popović, Nikola and Brdarić, Emilija and Đokić, Jelena and Dinić, Miroslav and Veljović, Katarina and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2020",
abstract = "Yogurt is a traditional fermented dairy product, prepared with starter cultures containing Streptococcus thermophilus and Lactobacillus bulgaricus that has gained widespread consumer acceptance as a healthy food. It is widely accepted that yogurt cultures have been recognized as probiotics, due to their beneficial effects on human health. In this study, we have characterized technological and health-promoting properties of autochthonous strains S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 isolated from artisanal sour milk and yogurt, respectively, in order to be used as functional yogurt starter cultures. Both BGKMJ1-36 and BGVLJ1-21 strains have the ability to form curd after five hours at 42 degrees C, hydrolyze alpha(s1)-, beta-, and kappa- casein, and to show antimicrobial activity toward Listeria monocytogenes. The strain BGKMJ1-36 produces exopolysaccharides important for rheological properties of the yogurt. The colonies of BGKMJ1-36 and BGVLJ1-21 strains that successfully survived transit of the yogurt through simulated gastrointestinal tract conditions have been tested for adhesion to intestinal epithelial Caco-2 cells. The results reveal that both strains adhere to Caco-2 cells and significantly upregulate the expression of autophagy-, tight junction proteins-, and anti-microbial peptides-related genes. Hence, both strains may be interesting for use as a novel functional starter culture for production of added-value yogurt with health-promoting properties.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro",
number = "10",
volume = "8",
doi = "10.3390/microorganisms8101586"
}

Popović, N., Brdarić, E., Đokić, J., Dinić, M., Veljović, K., Golić, N.,& Terzić-Vidojević, A.. (2020). Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. in Microorganisms
MDPI, Basel., 8(10).
https://doi.org/10.3390/microorganisms8101586

Popović N, Brdarić E, Đokić J, Dinić M, Veljović K, Golić N, Terzić-Vidojević A. Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. in Microorganisms. 2020;8(10).
doi:10.3390/microorganisms8101586 .

Popović, Nikola, Brdarić, Emilija, Đokić, Jelena, Dinić, Miroslav, Veljović, Katarina, Golić, Nataša, Terzić-Vidojević, Amarela, "Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro" in Microorganisms, 8, no. 10 (2020),
https://doi.org/10.3390/microorganisms8101586 . .

TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1395
AB  - Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - Journal of Clinical Gastroenterology
T1  - The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11
EP  - S12
SP  - S11
VL  - 54
DO  - 10.1097/MCG.0000000000001292
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1395
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2020",
abstract = "Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "Journal of Clinical Gastroenterology",
title = "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11",
pages = "S12-S11",
volume = "54",
doi = "10.1097/MCG.0000000000001292",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1395"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2020). The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology
Wolters Kluwer Health., 54, S11-S12.
https://doi.org/10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology. 2020;54:S11-S12.
doi:10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11" in Journal of Clinical Gastroenterology, 54 (2020):S11-S12,
https://doi.org/10.1097/MCG.0000000000001292 .,
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Brdarić, Emilija
AU  - Dinić, Miroslav
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Veljović, Katarina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1279
AB  - Listeria monocytogenes, the common foodborne pathogenic bacteria species, compromises the intestinal epithelial barrier, leading to development of the listeriosis, a severe disease especially among immunocompromised individuals. L. monocytogenes infection usually requires antibiotic treatment, however, excessive use of antibiotics promotes emergence of antibiotic resistance and the destruction of gut microbiota. Probiotics, including lactic acid bacteria (LAB), have been repeatedly proven as an alternative approach for the treatment of various infections. We have analyzed the potential of Enterococcus faecium BGPAS1-3, a dairy isolate exhibiting strong direct antilisterial effect, to modulate the response of differentiated Caco-2 intestinal epithelial cells to L. monocytogenes ATCC 19111 infection. We showed that the molecule with antilisterial effect is a bacterial cell-wall protein that is highly resistant to the high-temperature treatment. When we tested the antilisterial potential of heat-killed BGPAS1-3, we found that it could prevent tight junction disruption in differentiated Caco-2 monolayer infected with L. monocytogenes ATCC 19111, induce antilisterial host response mechanisms, and stimulate the production of protective TGF-beta in intestinal epithelial cells. We also showed that the modulation of MyD88 dependent TLR2 and TLR4 pathways by BGPAS1-3 are involved in host response against L. monocytogenes ATCC 19111. Since heat-killed BGPAS1-3 possess strong antilisterial effects, such postbiotic could be used as a controllable and safe therapeutic.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111
VL  - 10
DO  - 10.3389/fmicb.2019.00412
ER  - 

@article{
author = "Popović, Nikola and Đokić, Jelena and Brdarić, Emilija and Dinić, Miroslav and Terzić-Vidojević, Amarela and Golić, Nataša and Veljović, Katarina",
year = "2019",
abstract = "Listeria monocytogenes, the common foodborne pathogenic bacteria species, compromises the intestinal epithelial barrier, leading to development of the listeriosis, a severe disease especially among immunocompromised individuals. L. monocytogenes infection usually requires antibiotic treatment, however, excessive use of antibiotics promotes emergence of antibiotic resistance and the destruction of gut microbiota. Probiotics, including lactic acid bacteria (LAB), have been repeatedly proven as an alternative approach for the treatment of various infections. We have analyzed the potential of Enterococcus faecium BGPAS1-3, a dairy isolate exhibiting strong direct antilisterial effect, to modulate the response of differentiated Caco-2 intestinal epithelial cells to L. monocytogenes ATCC 19111 infection. We showed that the molecule with antilisterial effect is a bacterial cell-wall protein that is highly resistant to the high-temperature treatment. When we tested the antilisterial potential of heat-killed BGPAS1-3, we found that it could prevent tight junction disruption in differentiated Caco-2 monolayer infected with L. monocytogenes ATCC 19111, induce antilisterial host response mechanisms, and stimulate the production of protective TGF-beta in intestinal epithelial cells. We also showed that the modulation of MyD88 dependent TLR2 and TLR4 pathways by BGPAS1-3 are involved in host response against L. monocytogenes ATCC 19111. Since heat-killed BGPAS1-3 possess strong antilisterial effects, such postbiotic could be used as a controllable and safe therapeutic.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111",
volume = "10",
doi = "10.3389/fmicb.2019.00412"
}

Popović, N., Đokić, J., Brdarić, E., Dinić, M., Terzić-Vidojević, A., Golić, N.,& Veljović, K.. (2019). The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00412

Popović N, Đokić J, Brdarić E, Dinić M, Terzić-Vidojević A, Golić N, Veljović K. The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00412 .

Popović, Nikola, Đokić, Jelena, Brdarić, Emilija, Dinić, Miroslav, Terzić-Vidojević, Amarela, Golić, Nataša, Veljović, Katarina, "The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00412 . .

TY  - THES
AU  - Popović, Nikola
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6894
UR  - https://nardus.mpn.gov.rs/handle/123456789/11469
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20215/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025226930
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/57
AB  - Enterokoke pripadaju grupi mleĉnokiselinskih bakterija koje su široko rasprostranjene u prirodi. Predominantno se nalaze u gastrointestinalnom traktu životinja, od insekata do ĉoveka i preko fecesa se oslobaĊaju u spoljašnju sredinu, nakon ĉega naseljavaju razliĉita staništa. Imaju kontroverzni status zbog uloge u bolniĉkim infekcijama i širenja gena odgovornih za rezistenciju na antibiotike. S druge strane, neki sojevi se koriste kao probiotici za životinje i ĉoveka, kao i starter kulture u mlekarskoj industriji. Da bi se enterokoke koristile kao probiotici neophodno je da budu bezbedne za korišćenje, da nemaju hemolitiĉku i želatinoznu aktivnost i da nisu otporne na antibiotike. Enterokoke sintetišu antimikrobne proteine, bakteriocine, i na taj naĉin regulišu brojnost drugih bakterija i modulišu odgovor domaćina na infekciju. One mogu da sintetišu i biomolekule koji interaguju sa ćelijama domaćina i na taj naĉin mogu da menjaju razliĉite signalne puteve unutar ćelija domaćina. Biomolekuli sintetisani od strane probiotiĉkih enterokoka nazivaju se postbiotici. Primena postbiotika predstavlja bezbednu alternativu korišćenja enterokoka u biomedicini. Ova doktorska disertacija imala ja za cilj da ispita probiotiĉka i imunomodulatorna svojstva prirodnih sojeva enterokoka izolovanih iz fermentisanih mleĉnih proizvoda sa podruĉja Zapadnog Balkana. Definisana su tri cilja: 1. da se pronaĊu sojevi bezbedni za korišćenje u biomedicini; 2. da se ispitaju probiotiĉke karakteristike tih sojeva u modelima in vitro; i 3. da se ispita imunomodulatorni efekat odabranog soja u modelima in vitro i in vivo. U cilju ispitivanja bezbedne upotrebe enterokoka kao probiotika testirano je ukupno 75 sojeva iz fermentisanih mleĉnih proizvoda koje pripadaju vrstama: Enterococcus durans (50 sojeva), Enterococcus faecium (15 sojeva), Enterococcus faecalis (6 sojeva), Enterococcus italicus (3 soja) i Enterococcus hirae (1 soj). Hemolitiĉku aktivnost pokazalo je 18,7% (14/75), dok je želatinaznu aktivnost imalo 6,7% (5/75) testiranih sojeva. Na osnovu rezultata testa mikrodilucije pokazana je visoka uĉestalost rezistencije na ciprofloksacin 48,2% (27/56), dok su u manjoj meri testirani sojevi rezistentni na gentamicin 10,7% (6/56). Na osnovu rezultata sposobnosti formiranja biofilma u primenjenim uslovima, devet sojeva nema sposobnost formiranja biofilma, 11 sojeva ima mogućnost formiranja slabog biofilma, BGGO9-28 ima sposobnost formiranja jakog biofilma, dok sojevi BGTRK4-42 i BGZLM1-5 pokazuju veoma jaku sposobnost formiranja...
AB  - are predominantly found in the gastrointestinal tract of animals, from insects to humans and through faeces they are released into the environment, where they colonize different habitats. They have a controversial status according to the role as causative agents of hospital infections and the spread of genetic determinants of antibiotic resistance. In contrary, some strains have been used used as probiotics for animals and humans, as well as a starter cultures in the dairy industry. In order to use enterococci as probiotics, it is necessary to determine their safety, i.e. absence of hemolytic and gelatinase activity and susceptiblity to clinicaly relevant antibiotics. Enterococci synthesize antimicrobial proteins, bacteriocins, by which way they regulate the number of other bacteria and modulate the host response to the infection. They can also synthesize biomolecules that interact with the host cells, and change the different signalling pathways within host cells. Biomolecules synthesized by probiotic enterococci are called postbiotics. The use of postbiotic represents a safety alternative to the enterococci application in biomedicine. The aim of this dissertation was examination of probiotic and immunomodulatory characteristics of enterococci isolated from fermented dairy products from the Western Balkans countries. Three objectives were defined: 1. to find strains safe for use in biomedicine; 2. to examine the probiotic characteristics on in vitro models, and 3. to examine the immunomodulatory effect of the selected strain on in vitro and in vivo models. In order to analyse the safe use of enterococci as a probiotics, a total of 75 isolates from fermented dairy products were tested: Enterococcus durans (50 isolates), En. faecium (15 isolates), En. faecalis (6 isolates), En. italicus (3 isolates) and En. hirae (1 isolate). 18.7% (14/75) strains showed hemolytic activity, while 6.7% (5/75) had gelatinase activity. Based on the results of the microdilution test, 48.2% (27/56) of strains were ciprofloxacin resistance, while 10.7% (6/56) strains were resistant to gentamicin. According to the analysis of selected enterococci genomes, high incidence of adhesin encoding genes could be noticed. It was found that 30.4% of strains have three genes encoding different virulence factors, 21.7% of strains having two or four genes, 17.4% having five genes, and 4.3% of studied enterococci containing 6 or 7 genes which encode virulence factors, while the presence or absence of gene within the fsr operon varies from the analyzed strains...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo
T1  - Examination of probiotic and immunomodulatory characteristics of natural isolates of enterococci on in vitro and in vivo models
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11469
ER  - 

@phdthesis{
author = "Popović, Nikola",
year = "2019",
abstract = "Enterokoke pripadaju grupi mleĉnokiselinskih bakterija koje su široko rasprostranjene u prirodi. Predominantno se nalaze u gastrointestinalnom traktu životinja, od insekata do ĉoveka i preko fecesa se oslobaĊaju u spoljašnju sredinu, nakon ĉega naseljavaju razliĉita staništa. Imaju kontroverzni status zbog uloge u bolniĉkim infekcijama i širenja gena odgovornih za rezistenciju na antibiotike. S druge strane, neki sojevi se koriste kao probiotici za životinje i ĉoveka, kao i starter kulture u mlekarskoj industriji. Da bi se enterokoke koristile kao probiotici neophodno je da budu bezbedne za korišćenje, da nemaju hemolitiĉku i želatinoznu aktivnost i da nisu otporne na antibiotike. Enterokoke sintetišu antimikrobne proteine, bakteriocine, i na taj naĉin regulišu brojnost drugih bakterija i modulišu odgovor domaćina na infekciju. One mogu da sintetišu i biomolekule koji interaguju sa ćelijama domaćina i na taj naĉin mogu da menjaju razliĉite signalne puteve unutar ćelija domaćina. Biomolekuli sintetisani od strane probiotiĉkih enterokoka nazivaju se postbiotici. Primena postbiotika predstavlja bezbednu alternativu korišćenja enterokoka u biomedicini. Ova doktorska disertacija imala ja za cilj da ispita probiotiĉka i imunomodulatorna svojstva prirodnih sojeva enterokoka izolovanih iz fermentisanih mleĉnih proizvoda sa podruĉja Zapadnog Balkana. Definisana su tri cilja: 1. da se pronaĊu sojevi bezbedni za korišćenje u biomedicini; 2. da se ispitaju probiotiĉke karakteristike tih sojeva u modelima in vitro; i 3. da se ispita imunomodulatorni efekat odabranog soja u modelima in vitro i in vivo. U cilju ispitivanja bezbedne upotrebe enterokoka kao probiotika testirano je ukupno 75 sojeva iz fermentisanih mleĉnih proizvoda koje pripadaju vrstama: Enterococcus durans (50 sojeva), Enterococcus faecium (15 sojeva), Enterococcus faecalis (6 sojeva), Enterococcus italicus (3 soja) i Enterococcus hirae (1 soj). Hemolitiĉku aktivnost pokazalo je 18,7% (14/75), dok je želatinaznu aktivnost imalo 6,7% (5/75) testiranih sojeva. Na osnovu rezultata testa mikrodilucije pokazana je visoka uĉestalost rezistencije na ciprofloksacin 48,2% (27/56), dok su u manjoj meri testirani sojevi rezistentni na gentamicin 10,7% (6/56). Na osnovu rezultata sposobnosti formiranja biofilma u primenjenim uslovima, devet sojeva nema sposobnost formiranja biofilma, 11 sojeva ima mogućnost formiranja slabog biofilma, BGGO9-28 ima sposobnost formiranja jakog biofilma, dok sojevi BGTRK4-42 i BGZLM1-5 pokazuju veoma jaku sposobnost formiranja..., are predominantly found in the gastrointestinal tract of animals, from insects to humans and through faeces they are released into the environment, where they colonize different habitats. They have a controversial status according to the role as causative agents of hospital infections and the spread of genetic determinants of antibiotic resistance. In contrary, some strains have been used used as probiotics for animals and humans, as well as a starter cultures in the dairy industry. In order to use enterococci as probiotics, it is necessary to determine their safety, i.e. absence of hemolytic and gelatinase activity and susceptiblity to clinicaly relevant antibiotics. Enterococci synthesize antimicrobial proteins, bacteriocins, by which way they regulate the number of other bacteria and modulate the host response to the infection. They can also synthesize biomolecules that interact with the host cells, and change the different signalling pathways within host cells. Biomolecules synthesized by probiotic enterococci are called postbiotics. The use of postbiotic represents a safety alternative to the enterococci application in biomedicine. The aim of this dissertation was examination of probiotic and immunomodulatory characteristics of enterococci isolated from fermented dairy products from the Western Balkans countries. Three objectives were defined: 1. to find strains safe for use in biomedicine; 2. to examine the probiotic characteristics on in vitro models, and 3. to examine the immunomodulatory effect of the selected strain on in vitro and in vivo models. In order to analyse the safe use of enterococci as a probiotics, a total of 75 isolates from fermented dairy products were tested: Enterococcus durans (50 isolates), En. faecium (15 isolates), En. faecalis (6 isolates), En. italicus (3 isolates) and En. hirae (1 isolate). 18.7% (14/75) strains showed hemolytic activity, while 6.7% (5/75) had gelatinase activity. Based on the results of the microdilution test, 48.2% (27/56) of strains were ciprofloxacin resistance, while 10.7% (6/56) strains were resistant to gentamicin. According to the analysis of selected enterococci genomes, high incidence of adhesin encoding genes could be noticed. It was found that 30.4% of strains have three genes encoding different virulence factors, 21.7% of strains having two or four genes, 17.4% having five genes, and 4.3% of studied enterococci containing 6 or 7 genes which encode virulence factors, while the presence or absence of gene within the fsr operon varies from the analyzed strains...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo, Examination of probiotic and immunomodulatory characteristics of natural isolates of enterococci on in vitro and in vivo models",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11469"
}

Popović, N.. (2019). Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_11469

Popović N. Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11469 .

Popović, Nikola, "Ispitivanje probiotičkih i imunomodularnih karakteristika prirodnih izolata enterokoka u modelima in vitro i in vivo" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11469 .

TY  - JOUR
AU  - Popović, Nikola
AU  - Dinić, Miroslav
AU  - Tolinački, Maja
AU  - Mihajlović, Sanja
AU  - Terzić-Vidojević, Amarela
AU  - Bojić, Svetlana
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Veljović, Katarina
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1157
AB  - Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates
VL  - 9
DO  - 10.3389/fmicb.2018.00078
ER  - 

@article{
author = "Popović, Nikola and Dinić, Miroslav and Tolinački, Maja and Mihajlović, Sanja and Terzić-Vidojević, Amarela and Bojić, Svetlana and Đokić, Jelena and Golić, Nataša and Veljović, Katarina",
year = "2018",
abstract = "Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates",
volume = "9",
doi = "10.3389/fmicb.2018.00078"
}

Popović, N., Dinić, M., Tolinački, M., Mihajlović, S., Terzić-Vidojević, A., Bojić, S., Đokić, J., Golić, N.,& Veljović, K.. (2018). New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.00078

Popović N, Dinić M, Tolinački M, Mihajlović S, Terzić-Vidojević A, Bojić S, Đokić J, Golić N, Veljović K. New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.00078 .

Popović, Nikola, Dinić, Miroslav, Tolinački, Maja, Mihajlović, Sanja, Terzić-Vidojević, Amarela, Bojić, Svetlana, Đokić, Jelena, Golić, Nataša, Veljović, Katarina, "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.00078 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Tolinački, Maja
AU  - Terzić-Vidojević, Amarela
AU  - Kojić, Milan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/997
AB  - The understanding of mechanisms of interactions between various bacterial cell surface proteins and host receptors has become imperative for the study of the health promoting features of probiotic enterococci. This study, for the first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faeciurn BGGO9-28, selected from a laboratory collection of enterococcal isolates with auto aggregation phenotypes. Among them, En. faecium BGG09-28 showed the strongest auto -aggregation, adhesion to components of ECM and biofilm formation. Novel aggregation promoting factor AggE, a protein of 178.1 kDa, belongs to the collagen -binding superfamily of proteins and shares similar architecture with previously discovered aggregation factors from lactic acid bacteria (LAB). Its expression in heterologous enterococcal and lactococcal hosts demonstrates that the aggE gene is sufficient for cell aggregation. The derivatives carrying aggE exhibited the ten times higher adhesion ability to collagen and fibronectin, possess about two times higher adhesion to mucin and contribute to the increase of biofilm formation, comparing to the control strains. Analysis for the presence of virulence factors (cytolysin and gelatinase production), antibiotic resistance (antibiotic susceptibility) and genes (cylA, egg, gelE, esp, hyiN, ace, efaks, and efagn) showed that BGG09-28 was sensitive to all tested antibiotics, without hemolytic or gelatinase activity. This strain does not carry any of the tested genes encoding for known virulence factors. Results showed that BGGO9-28 was resistant to low pH and high concentrations of bile salts. Also, it adhered strongly to the Caco-2 human epithelial cell line. In conclusion, the results of this study indicate that the presence of AggE protein on the cell surface in enterococci is a desirable probiotic feature.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28
VL  - 8
DO  - 10.3389/fmicb.2017.01843
ER  - 

@article{
author = "Veljović, Katarina and Popović, Nikola and Miljković, Marija and Tolinački, Maja and Terzić-Vidojević, Amarela and Kojić, Milan",
year = "2017",
abstract = "The understanding of mechanisms of interactions between various bacterial cell surface proteins and host receptors has become imperative for the study of the health promoting features of probiotic enterococci. This study, for the first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faeciurn BGGO9-28, selected from a laboratory collection of enterococcal isolates with auto aggregation phenotypes. Among them, En. faecium BGG09-28 showed the strongest auto -aggregation, adhesion to components of ECM and biofilm formation. Novel aggregation promoting factor AggE, a protein of 178.1 kDa, belongs to the collagen -binding superfamily of proteins and shares similar architecture with previously discovered aggregation factors from lactic acid bacteria (LAB). Its expression in heterologous enterococcal and lactococcal hosts demonstrates that the aggE gene is sufficient for cell aggregation. The derivatives carrying aggE exhibited the ten times higher adhesion ability to collagen and fibronectin, possess about two times higher adhesion to mucin and contribute to the increase of biofilm formation, comparing to the control strains. Analysis for the presence of virulence factors (cytolysin and gelatinase production), antibiotic resistance (antibiotic susceptibility) and genes (cylA, egg, gelE, esp, hyiN, ace, efaks, and efagn) showed that BGG09-28 was sensitive to all tested antibiotics, without hemolytic or gelatinase activity. This strain does not carry any of the tested genes encoding for known virulence factors. Results showed that BGGO9-28 was resistant to low pH and high concentrations of bile salts. Also, it adhered strongly to the Caco-2 human epithelial cell line. In conclusion, the results of this study indicate that the presence of AggE protein on the cell surface in enterococci is a desirable probiotic feature.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28",
volume = "8",
doi = "10.3389/fmicb.2017.01843"
}

Veljović, K., Popović, N., Miljković, M., Tolinački, M., Terzić-Vidojević, A.,& Kojić, M.. (2017). Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.01843

Veljović K, Popović N, Miljković M, Tolinački M, Terzić-Vidojević A, Kojić M. Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.01843 .

Veljović, Katarina, Popović, Nikola, Miljković, Marija, Tolinački, Maja, Terzić-Vidojević, Amarela, Kojić, Milan, "Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.01843 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Strahinić, Ivana
AU  - Mrvaljević, Igor
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1091
AB  - Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.
PB  - BMC, London
T2  - BMC Microbiology
T1  - In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
VL  - 17
DO  - 10.1186/s12866-017-1015-5
ER  - 

@article{
author = "Golić, Nataša and Veljović, Katarina and Popović, Nikola and Đokić, Jelena and Strahinić, Ivana and Mrvaljević, Igor and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.",
publisher = "BMC, London",
journal = "BMC Microbiology",
title = "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens",
volume = "17",
doi = "10.1186/s12866-017-1015-5"
}

Golić, N., Veljović, K., Popović, N., Đokić, J., Strahinić, I., Mrvaljević, I.,& Terzić-Vidojević, A.. (2017). In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology
BMC, London., 17.
https://doi.org/10.1186/s12866-017-1015-5

Golić N, Veljović K, Popović N, Đokić J, Strahinić I, Mrvaljević I, Terzić-Vidojević A. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology. 2017;17.
doi:10.1186/s12866-017-1015-5 .

Golić, Nataša, Veljović, Katarina, Popović, Nikola, Đokić, Jelena, Strahinić, Ivana, Mrvaljević, Igor, Terzić-Vidojević, Amarela, "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens" in BMC Microbiology, 17 (2017),
https://doi.org/10.1186/s12866-017-1015-5 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Mihajlović, Sanja
AU  - Jovčić, Branko
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/818
AB  - Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia
IS  - 9
VL  - 187
DO  - 10.1007/s10661-015-4814-x
ER  - 

@article{
author = "Veljović, Katarina and Popović, Nikola and Terzić-Vidojević, Amarela and Tolinački, Maja and Mihajlović, Sanja and Jovčić, Branko and Kojić, Milan",
year = "2015",
abstract = "Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia",
number = "9",
volume = "187",
doi = "10.1007/s10661-015-4814-x"
}

Veljović, K., Popović, N., Terzić-Vidojević, A., Tolinački, M., Mihajlović, S., Jovčić, B.,& Kojić, M.. (2015). Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 187(9).
https://doi.org/10.1007/s10661-015-4814-x

Veljović K, Popović N, Terzić-Vidojević A, Tolinački M, Mihajlović S, Jovčić B, Kojić M. Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2015;187(9).
doi:10.1007/s10661-015-4814-x .

Veljović, Katarina, Popović, Nikola, Terzić-Vidojević, Amarela, Tolinački, Maja, Mihajlović, Sanja, Jovčić, Branko, Kojić, Milan, "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia" in Environmental Monitoring and Assessment, 187, no. 9 (2015),
https://doi.org/10.1007/s10661-015-4814-x . .