TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puskas, Nela
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1101
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
PB  - IOS Press, Amsterdam
T2  - Journal of Alzheimers Disease
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors
EP  - 976
IS  - 3
SP  - 963
VL  - 65
DO  - 10.3233/JAD-180277
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puskas, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
publisher = "IOS Press, Amsterdam",
journal = "Journal of Alzheimers Disease",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors",
pages = "976-963",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puskas, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors. in Journal of Alzheimers Disease
IOS Press, Amsterdam., 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puskas N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors. in Journal of Alzheimers Disease. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puskas, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors" in Journal of Alzheimers Disease, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .

TY  - JOUR
AU  - Tesić, Vesna
AU  - Perović, Milka
AU  - Lazić, Divna
AU  - Kojić, Snežana
AU  - Smiljanić, Kosara
AU  - Ruzdijić, Sabera
AU  - Rakić, Ljubisav
AU  - Kanazir, Selma
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/880
AB  - Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat
EP  - 52
SP  - 43
VL  - 149
DO  - 10.1016/j.jsbmb.2015.01.013
ER  - 

@article{
author = "Tesić, Vesna and Perović, Milka and Lazić, Divna and Kojić, Snežana and Smiljanić, Kosara and Ruzdijić, Sabera and Rakić, Ljubisav and Kanazir, Selma",
year = "2015",
abstract = "Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat",
pages = "52-43",
volume = "149",
doi = "10.1016/j.jsbmb.2015.01.013"
}

Tesić, V., Perović, M., Lazić, D., Kojić, S., Smiljanić, K., Ruzdijić, S., Rakić, L.,& Kanazir, S.. (2015). Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology
Pergamon-Elsevier Science Ltd, Oxford., 149, 43-52.
https://doi.org/10.1016/j.jsbmb.2015.01.013

Tesić V, Perović M, Lazić D, Kojić S, Smiljanić K, Ruzdijić S, Rakić L, Kanazir S. Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology. 2015;149:43-52.
doi:10.1016/j.jsbmb.2015.01.013 .

Tesić, Vesna, Perović, Milka, Lazić, Divna, Kojić, Snežana, Smiljanić, Kosara, Ruzdijić, Sabera, Rakić, Ljubisav, Kanazir, Selma, "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat" in Journal of Steroid Biochemistry and Molecular Biology, 149 (2015):43-52,
https://doi.org/10.1016/j.jsbmb.2015.01.013 . .