TY  - CONF
AU  - Demir, Arnela
AU  - Selović, Alisa
AU  - Dahija, Sabina
AU  - Parić, Adisa
AU  - Samardžić, Jelena
AU  - Karalija, Erna
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1906
AB  - To improve our understanding of the molecular mechanisms underlying seed priming, RNA
transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed
priming was performed by submerging the seeds in different priming agents for 24h at 4°C, followed
by rinsing with sterilized water and desiccation to the original moisture content. Silene sendtneri is a
species with no sequenced genome and annotation of de novo assembly of the transcriptome was done
using different species for the reference genome. Gene ontology (GO) analysis indicated that genes
related to heavy metal transporters and heat shock proteins are differentially expressed after priming in
relation to the used priming agent. Within these gene categories, genes such as heavy metal-associated
isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy
metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5,98) were
upregulated. Some gene categories are commonly highly expressed after seed priming regardless of the
used agent, but some specific gene categories have been recorded to be expressed in relation to the used
agent.
PB  - Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Genetics & Applications
T1  - Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression
IS  - 2 (Special edition)
SP  - 143
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1906
ER  - 

@conference{
author = "Demir, Arnela and Selović, Alisa and Dahija, Sabina and Parić, Adisa and Samardžić, Jelena and Karalija, Erna",
year = "2023",
abstract = "To improve our understanding of the molecular mechanisms underlying seed priming, RNA
transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed
priming was performed by submerging the seeds in different priming agents for 24h at 4°C, followed
by rinsing with sterilized water and desiccation to the original moisture content. Silene sendtneri is a
species with no sequenced genome and annotation of de novo assembly of the transcriptome was done
using different species for the reference genome. Gene ontology (GO) analysis indicated that genes
related to heavy metal transporters and heat shock proteins are differentially expressed after priming in
relation to the used priming agent. Within these gene categories, genes such as heavy metal-associated
isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy
metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5,98) were
upregulated. Some gene categories are commonly highly expressed after seed priming regardless of the
used agent, but some specific gene categories have been recorded to be expressed in relation to the used
agent.",
publisher = "Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Genetics & Applications",
title = "Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression",
number = "2 (Special edition)",
pages = "143",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1906"
}

Demir, A., Selović, A., Dahija, S., Parić, A., Samardžić, J.,& Karalija, E.. (2023). Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression. in Genetics & Applications
Sarajevo : Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 7(2 (Special edition)), 143.
https://hdl.handle.net/21.15107/rcub_imagine_1906

Demir A, Selović A, Dahija S, Parić A, Samardžić J, Karalija E. Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression. in Genetics & Applications. 2023;7(2 (Special edition)):143.
https://hdl.handle.net/21.15107/rcub_imagine_1906 .

Demir, Arnela, Selović, Alisa, Dahija, Sabina, Parić, Adisa, Samardžić, Jelena, Karalija, Erna, "Seed priming enhances tolerance and accumulation of heavy metals in cd hyperaccumulator silene sendtneri through changes in gene expression" in Genetics & Applications, 7, no. 2 (Special edition) (2023):143,
https://hdl.handle.net/21.15107/rcub_imagine_1906 .

TY  - JOUR
AU  - Karalija, Erna
AU  - Selović, Alisa
AU  - Dahija, Sabina
AU  - Demir, Arnela
AU  - Samardžić, Jelena
AU  - Vrobel, Ondrej
AU  - Cavar-Zeljković, Sanja
AU  - Parić, Adisa
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1488
AB  - Changes in the environment as a result of industrialisation and urbanisation impact negatively on plant growth and crop production. Cadmium (Cd) is one of the most dangerous metals that enters the food chain, with toxic effects on plants and human health. This study evaluated the potential of Silene sendtneri as a novel hyperaccumulator and the role of seed priming in tolerance and accumulation rate of Cd. The effect of different priming agents on germination performance, root growth, seedling development, metal uptake and accumulation, antioxidant defences including enzymatic and non-enzymatic antioxidants has been assessed. Seed priming using silicic acid, proline alone or in combination with salicylic acidenhanced germination, seedling development, and root growth under Cd stress. The same priming treatments induced an increase of water content in shoots and roots when plants were exposed to Cd. The enzymatic antioxidant response was specific for the priming agent used. An increase in ferulic acid and rutin in shoots was related to the increase of Cd concentration in the medium. The concentration of malic and oxalic acid increased significantly in shoots of plants grown on high Cd concentrations compared to low Cd concentrations. Silene sendtneri can accumulate significant levels of Cd with enhanced accumulation rate and tolerance when seeds are primed. The best results are obtained by seed priming using 1% silicic acid, proline and salicylic acid.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator
VL  - 210
DO  - 10.1016/j.ecoenv.2020.111882
ER  - 

@article{
author = "Karalija, Erna and Selović, Alisa and Dahija, Sabina and Demir, Arnela and Samardžić, Jelena and Vrobel, Ondrej and Cavar-Zeljković, Sanja and Parić, Adisa",
year = "2021",
abstract = "Changes in the environment as a result of industrialisation and urbanisation impact negatively on plant growth and crop production. Cadmium (Cd) is one of the most dangerous metals that enters the food chain, with toxic effects on plants and human health. This study evaluated the potential of Silene sendtneri as a novel hyperaccumulator and the role of seed priming in tolerance and accumulation rate of Cd. The effect of different priming agents on germination performance, root growth, seedling development, metal uptake and accumulation, antioxidant defences including enzymatic and non-enzymatic antioxidants has been assessed. Seed priming using silicic acid, proline alone or in combination with salicylic acidenhanced germination, seedling development, and root growth under Cd stress. The same priming treatments induced an increase of water content in shoots and roots when plants were exposed to Cd. The enzymatic antioxidant response was specific for the priming agent used. An increase in ferulic acid and rutin in shoots was related to the increase of Cd concentration in the medium. The concentration of malic and oxalic acid increased significantly in shoots of plants grown on high Cd concentrations compared to low Cd concentrations. Silene sendtneri can accumulate significant levels of Cd with enhanced accumulation rate and tolerance when seeds are primed. The best results are obtained by seed priming using 1% silicic acid, proline and salicylic acid.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator",
volume = "210",
doi = "10.1016/j.ecoenv.2020.111882"
}

Karalija, E., Selović, A., Dahija, S., Demir, A., Samardžić, J., Vrobel, O., Cavar-Zeljković, S.,& Parić, A.. (2021). Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 210.
https://doi.org/10.1016/j.ecoenv.2020.111882

Karalija E, Selović A, Dahija S, Demir A, Samardžić J, Vrobel O, Cavar-Zeljković S, Parić A. Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator. in Ecotoxicology and Environmental Safety. 2021;210.
doi:10.1016/j.ecoenv.2020.111882 .

Karalija, Erna, Selović, Alisa, Dahija, Sabina, Demir, Arnela, Samardžić, Jelena, Vrobel, Ondrej, Cavar-Zeljković, Sanja, Parić, Adisa, "Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator" in Ecotoxicology and Environmental Safety, 210 (2021),
https://doi.org/10.1016/j.ecoenv.2020.111882 . .