TY  - CONF
AU  - Baralić, Katarina
AU  - Pavić, Aleksandar
AU  - Javorac, Dragana
AU  - Živančević, Katarina
AU  - Božić, Dragica
AU  - Radaković, Nataša
AU  - Antonijević Miljaković, Evica
AU  - Buha Djordjevic, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2283
AB  - The extensive usage of bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A
(BPA) creates a lot of opportunities for combined human exposure to these hazardous compounds in
everyday life and a variety of negative outcomes, including hepatotoxicity. In silico research and two
in vivo models were used to investigate the links between a mixture including DEHP, DBP and BPA and
liver injury. Bioinformatic analysis was performed by Comparative Toxicogenomics Database, ShinyGO,
ToppCluster, and Cytoscape. In vivo subacute study included five groups of rats (n = 6): (1) Control: corn
oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP
+ DBP + BPA. Zebrafish embryos were exposed to the investigated substances in multiple dosages, both
alone and in combination (binary and ternary mixtures). Liver damage was linked to 75 DEHP, DBP, and
BPA genes, the majority of which were associated with inflammation/oxidative stress, identified as the
most relevant molecular pathways. In rats, significant changes in redox status/bioelements’ level and
pathohistology were more pronounced or evident only in MIX group, suggesting probable additivity.
In a dose-dependent manner, BPA reduced the liver area (LA) index. LA values were decreased by DEHP
(2 μg/mL) and DBP (5 μg/mL), whereas LA index was raised by their higher concentrations. In binary
mixtures, DBP had a lethal effect at the two highest concentrations, whereas BPA directed hepatotoxicity
of the DEHP/DBP/BPA mixture.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
T1  - Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models
EP  - 56
SP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2283
ER  - 

@conference{
author = "Baralić, Katarina and Pavić, Aleksandar and Javorac, Dragana and Živančević, Katarina and Božić, Dragica and Radaković, Nataša and Antonijević Miljaković, Evica and Buha Djordjevic, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2023",
abstract = "The extensive usage of bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A
(BPA) creates a lot of opportunities for combined human exposure to these hazardous compounds in
everyday life and a variety of negative outcomes, including hepatotoxicity. In silico research and two
in vivo models were used to investigate the links between a mixture including DEHP, DBP and BPA and
liver injury. Bioinformatic analysis was performed by Comparative Toxicogenomics Database, ShinyGO,
ToppCluster, and Cytoscape. In vivo subacute study included five groups of rats (n = 6): (1) Control: corn
oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP
+ DBP + BPA. Zebrafish embryos were exposed to the investigated substances in multiple dosages, both
alone and in combination (binary and ternary mixtures). Liver damage was linked to 75 DEHP, DBP, and
BPA genes, the majority of which were associated with inflammation/oxidative stress, identified as the
most relevant molecular pathways. In rats, significant changes in redox status/bioelements’ level and
pathohistology were more pronounced or evident only in MIX group, suggesting probable additivity.
In a dose-dependent manner, BPA reduced the liver area (LA) index. LA values were decreased by DEHP
(2 μg/mL) and DBP (5 μg/mL), whereas LA index was raised by their higher concentrations. In binary
mixtures, DBP had a lethal effect at the two highest concentrations, whereas BPA directed hepatotoxicity
of the DEHP/DBP/BPA mixture.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities",
title = "Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models",
pages = "56-56",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2283"
}

Baralić, K., Pavić, A., Javorac, D., Živančević, K., Božić, D., Radaković, N., Antonijević Miljaković, E., Buha Djordjevic, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2023). Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
Beograd : Udruženje toksikologa Srbije., 56-56.
https://hdl.handle.net/21.15107/rcub_imagine_2283

Baralić K, Pavić A, Javorac D, Živančević K, Božić D, Radaković N, Antonijević Miljaković E, Buha Djordjevic A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:56-56.
https://hdl.handle.net/21.15107/rcub_imagine_2283 .

Baralić, Katarina, Pavić, Aleksandar, Javorac, Dragana, Živančević, Katarina, Božić, Dragica, Radaković, Nataša, Antonijević Miljaković, Evica, Buha Djordjevic, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Three lines of evidence of the hepatotoxicity young researchers of a mixture containing phthalates and bisphenol a: in silico and two in vivo models" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):56-56,
https://hdl.handle.net/21.15107/rcub_imagine_2283 .

TY  - CONF
AU  - Đurić, Ana
AU  - Srdić-Rajić, Tatjana
AU  - Božić, Dragica
AU  - Baralić, Katarina
AU  - Pavić, Aleksandar
AU  - Đukić-Ćosić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2284
AB  - Colorectal carcinoma (CRC) is an increasing cause of morbidity and mortality worldwide.
Among biologically active compounds which have been shown to exert cytotoxic effects on human cancer
cells, including CRC, is sulforaphane (SFN), an isothiocyanate compound extracted from cruciferous
vegetables, especially broccoli. This in vitro study aimed to investigate the effect of SFN on the growth
of human CRC cells and its dependency on the expression of p53. Two human CRC cell lines: HT-29
(a p53 mutated line) and HT-116 (a p53 wild-type line) were treated with SFN at concentrations of 0, 4,
8, 16, and 32 μmol/L for 72 h to test its anticancer effect.
Results indicated that SFN induced cell morphological changes and decreased the total number
of viable cells. Treatment of CRC cells with SFN for 72 h resulted in moderate dose-dependent cytotoxicity.
HCT-116 cells, with a p53-wt, were more sensitive (IC50 = 8.41 μM) than p53-mutated HT-29 cells (IC50
= 24.83 μM). These results indicate that SFN exhibits the anticancer effect against CRC cells in p53 mutation-
dependent manner (Serbia-China project: 451-03-1203/2021-09).
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
T1  - Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect
EP  - 180
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2284
ER  - 

@conference{
author = "Đurić, Ana and Srdić-Rajić, Tatjana and Božić, Dragica and Baralić, Katarina and Pavić, Aleksandar and Đukić-Ćosić, Danijela",
year = "2023",
abstract = "Colorectal carcinoma (CRC) is an increasing cause of morbidity and mortality worldwide.
Among biologically active compounds which have been shown to exert cytotoxic effects on human cancer
cells, including CRC, is sulforaphane (SFN), an isothiocyanate compound extracted from cruciferous
vegetables, especially broccoli. This in vitro study aimed to investigate the effect of SFN on the growth
of human CRC cells and its dependency on the expression of p53. Two human CRC cell lines: HT-29
(a p53 mutated line) and HT-116 (a p53 wild-type line) were treated with SFN at concentrations of 0, 4,
8, 16, and 32 μmol/L for 72 h to test its anticancer effect.
Results indicated that SFN induced cell morphological changes and decreased the total number
of viable cells. Treatment of CRC cells with SFN for 72 h resulted in moderate dose-dependent cytotoxicity.
HCT-116 cells, with a p53-wt, were more sensitive (IC50 = 8.41 μM) than p53-mutated HT-29 cells (IC50
= 24.83 μM). These results indicate that SFN exhibits the anticancer effect against CRC cells in p53 mutation-
dependent manner (Serbia-China project: 451-03-1203/2021-09).",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities",
title = "Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2284"
}

Đurić, A., Srdić-Rajić, T., Božić, D., Baralić, K., Pavić, A.,& Đukić-Ćosić, D.. (2023). Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
Beograd : Udruženje toksikologa Srbije., 180-180.
https://hdl.handle.net/21.15107/rcub_imagine_2284

Đurić A, Srdić-Rajić T, Božić D, Baralić K, Pavić A, Đukić-Ćosić D. Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:180-180.
https://hdl.handle.net/21.15107/rcub_imagine_2284 .

Đurić, Ana, Srdić-Rajić, Tatjana, Božić, Dragica, Baralić, Katarina, Pavić, Aleksandar, Đukić-Ćosić, Danijela, "Sulforaphane affects morphological changes and cell viability of colon cancer cells: p53 mutation-dependent effect" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):180-180,
https://hdl.handle.net/21.15107/rcub_imagine_2284 .

TY  - CONF
AU  - Pavić, Aleksandar
AU  - Radaković, Nataša
AU  - Srdić-Rajić, Tatjana
AU  - Božić, Dragica
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2282
AB  - Sulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants,
especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been
shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects,
antioxidant and immunomodulatory effects, as well as positive effects in various cancers.
However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for
chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane
on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently
labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used,
treated with different concentrations of SFN (1 to 20 μg/mL).
The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for
five days. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the
median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic
effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4
μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver
size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular
system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane
on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the
need for further investigation of toxicological potential of this molecule.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
T1  - Toxicity testing of D, L-sulforaphane in a zebrafish model
EP  - 254
SP  - 254
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2282
ER  - 

@conference{
author = "Pavić, Aleksandar and Radaković, Nataša and Srdić-Rajić, Tatjana and Božić, Dragica and Baralić, Katarina and Đukić-Ćosić, Danijela",
year = "2023",
abstract = "Sulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants,
especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been
shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects,
antioxidant and immunomodulatory effects, as well as positive effects in various cancers.
However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for
chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane
on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently
labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used,
treated with different concentrations of SFN (1 to 20 μg/mL).
The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for
five days. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the
median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic
effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4
μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver
size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular
system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane
on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the
need for further investigation of toxicological potential of this molecule.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities",
title = "Toxicity testing of D, L-sulforaphane in a zebrafish model",
pages = "254-254",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2282"
}

Pavić, A., Radaković, N., Srdić-Rajić, T., Božić, D., Baralić, K.,& Đukić-Ćosić, D.. (2023). Toxicity testing of D, L-sulforaphane in a zebrafish model. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities
Beograd : Udruženje toksikologa Srbije., 254-254.
https://hdl.handle.net/21.15107/rcub_imagine_2282

Pavić A, Radaković N, Srdić-Rajić T, Božić D, Baralić K, Đukić-Ćosić D. Toxicity testing of D, L-sulforaphane in a zebrafish model. in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities. 2023;:254-254.
https://hdl.handle.net/21.15107/rcub_imagine_2282 .

Pavić, Aleksandar, Radaković, Nataša, Srdić-Rajić, Tatjana, Božić, Dragica, Baralić, Katarina, Đukić-Ćosić, Danijela, "Toxicity testing of D, L-sulforaphane in a zebrafish model" in 13th International congress of the Serbian society of toxicology and 1. TOXSEE regional conference – present and future of toxicology: challenges and opportunities (2023):254-254,
https://hdl.handle.net/21.15107/rcub_imagine_2282 .

TY  - JOUR
AU  - Baralić, Katarina
AU  - Pavić, Aleksandar
AU  - Javorac, Dragana
AU  - Živančević, Katarina
AU  - Božić, Dragica
AU  - Radaković, Nataša
AU  - Antonijević Miljaković, Evica
AU  - Buha Djordjevic, Aleksandra
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1661
AB  - Connections between the mixture containing bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) and liver injury were explored through in silico investigation and 2 in vivo models. Comparative Toxicogenomics Database (CTD), ShinyGO, ToppCluster and Cytoscape were used for bioinformatic analysis. In vivo subacute study was performed on rats - five groups (n = 6): (1) Control: corn oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP + DBP + BPA. Zebrafish embryos were exposed to the investigated substances in different doses, singularly and combined (binary and ternary mixtures). Liver injury was linked to 75 DEHP, DBP, and BPA genes, mostly connected to inflammation/oxidative stress. In rats, significant alterations in redox status/bioelements and pathohistology were most notable or exclusively present in MIX (probable additive effects). BPA decreased liver area (LA) index in dose-dependent manner. DEHP (< 2 µg/mL) and DBP (≤ 5 µg/mL) reduced LA values, while their higher doses increased LA index. The effect of DBP in binary mixtures led to a lethal outcome at the two highest concentrations, while the hepatotoxicity of DEHP/DBP/BPA mixture was dictated by BPA (confirmed by the benchmark dose analysis).
T2  - Journal of Hazardous Materials
T2  - Journal of Hazardous Materials Journal of Hazardous Materials
T1  - Comprehensive investigation of hepatotoxicity of the mixture containing phthalates and bisphenol A
SP  - 130404
VL  - 445
DO  - 10.1016/j.jhazmat.2022.130404
ER  - 

@article{
author = "Baralić, Katarina and Pavić, Aleksandar and Javorac, Dragana and Živančević, Katarina and Božić, Dragica and Radaković, Nataša and Antonijević Miljaković, Evica and Buha Djordjevic, Aleksandra and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2023",
abstract = "Connections between the mixture containing bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) and liver injury were explored through in silico investigation and 2 in vivo models. Comparative Toxicogenomics Database (CTD), ShinyGO, ToppCluster and Cytoscape were used for bioinformatic analysis. In vivo subacute study was performed on rats - five groups (n = 6): (1) Control: corn oil, (2) DEHP: 50 mg/kg b.w./day, (3) DBP: 50 mg/kg b.w./day, (4) BPA: 25 mg/kg b.w./day, (5) MIX: DEHP + DBP + BPA. Zebrafish embryos were exposed to the investigated substances in different doses, singularly and combined (binary and ternary mixtures). Liver injury was linked to 75 DEHP, DBP, and BPA genes, mostly connected to inflammation/oxidative stress. In rats, significant alterations in redox status/bioelements and pathohistology were most notable or exclusively present in MIX (probable additive effects). BPA decreased liver area (LA) index in dose-dependent manner. DEHP (< 2 µg/mL) and DBP (≤ 5 µg/mL) reduced LA values, while their higher doses increased LA index. The effect of DBP in binary mixtures led to a lethal outcome at the two highest concentrations, while the hepatotoxicity of DEHP/DBP/BPA mixture was dictated by BPA (confirmed by the benchmark dose analysis).",
journal = "Journal of Hazardous Materials, Journal of Hazardous Materials Journal of Hazardous Materials",
title = "Comprehensive investigation of hepatotoxicity of the mixture containing phthalates and bisphenol A",
pages = "130404",
volume = "445",
doi = "10.1016/j.jhazmat.2022.130404"
}

Baralić, K., Pavić, A., Javorac, D., Živančević, K., Božić, D., Radaković, N., Antonijević Miljaković, E., Buha Djordjevic, A., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2023). Comprehensive investigation of hepatotoxicity of the mixture containing phthalates and bisphenol A. in Journal of Hazardous Materials, 445, 130404.
https://doi.org/10.1016/j.jhazmat.2022.130404

Baralić K, Pavić A, Javorac D, Živančević K, Božić D, Radaković N, Antonijević Miljaković E, Buha Djordjevic A, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Comprehensive investigation of hepatotoxicity of the mixture containing phthalates and bisphenol A. in Journal of Hazardous Materials. 2023;445:130404.
doi:10.1016/j.jhazmat.2022.130404 .

Baralić, Katarina, Pavić, Aleksandar, Javorac, Dragana, Živančević, Katarina, Božić, Dragica, Radaković, Nataša, Antonijević Miljaković, Evica, Buha Djordjevic, Aleksandra, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Comprehensive investigation of hepatotoxicity of the mixture containing phthalates and bisphenol A" in Journal of Hazardous Materials, 445 (2023):130404,
https://doi.org/10.1016/j.jhazmat.2022.130404 . .