TY  - JOUR
AU  - Olaifa, Kayode
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
AU  - Boschetto, Francesco
AU  - Marin, Elia
AU  - Segreto, Francesco
AU  - Marsili, Enrico
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1465
AB  - Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing
VL  - 389
DO  - 10.1016/j.electacta.2021.138757
ER  - 

@article{
author = "Olaifa, Kayode and Nikodinović-Runić, Jasmina and Glišić, Biljana and Boschetto, Francesco and Marin, Elia and Segreto, Francesco and Marsili, Enrico",
year = "2021",
abstract = "Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing",
volume = "389",
doi = "10.1016/j.electacta.2021.138757"
}

Olaifa, K., Nikodinović-Runić, J., Glišić, B., Boschetto, F., Marin, E., Segreto, F.,& Marsili, E.. (2021). Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford., 389.
https://doi.org/10.1016/j.electacta.2021.138757

Olaifa K, Nikodinović-Runić J, Glišić B, Boschetto F, Marin E, Segreto F, Marsili E. Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta. 2021;389.
doi:10.1016/j.electacta.2021.138757 .

Olaifa, Kayode, Nikodinović-Runić, Jasmina, Glišić, Biljana, Boschetto, Francesco, Marin, Elia, Segreto, Francesco, Marsili, Enrico, "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing" in Electrochimica Acta, 389 (2021),
https://doi.org/10.1016/j.electacta.2021.138757 . .