TY  - JOUR
AU  - Olaifa, Kayode
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
AU  - Boschetto, Francesco
AU  - Marin, Elia
AU  - Segreto, Francesco
AU  - Marsili, Enrico
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1465
AB  - Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing
VL  - 389
DO  - 10.1016/j.electacta.2021.138757
ER  - 

@article{
author = "Olaifa, Kayode and Nikodinović-Runić, Jasmina and Glišić, Biljana and Boschetto, Francesco and Marin, Elia and Segreto, Francesco and Marsili, Enrico",
year = "2021",
abstract = "Candida albicans is a fungal pathogen that accounts for more than a million death annually. Analysis of Candida biofilms and rapid assessment of antifungal therapy is a critical challenge in clinical practice. Current biochemical methods are time-consuming and expensive or require expert interpretation of the results. Hence, there is the need for a rapid anti-fungal and anti-biofilm test. While electroanalysis has been previously adopted to determine antibiotic susceptibility in bacterial biofilms, there are no studies on yeast and fungi. In this work, electroanalysis of C. albicans biofilm and evaluation of anti-biofilm activity of several antifungal compounds using screen-printed carbon electrodes (SPEs) are reported. Results are compared with standard biochemical and microscopic methods. The current output decreases by 47.5%, 73.4%, and 88.5% in biofilms treated with the antifungals fluconazole (Flz), amphotericin B (AmB), and complex Ag3, respectively, indicating the suitability of electrochemical testing for evaluation of anti fungal drugs. Chronoamperometry allows measuring antifungal activity on Candida biofilm as early as 10 h after inoculation, which show promises for the development of bioelectrochemical sensors in clinical settings.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing",
volume = "389",
doi = "10.1016/j.electacta.2021.138757"
}

Olaifa, K., Nikodinović-Runić, J., Glišić, B., Boschetto, F., Marin, E., Segreto, F.,& Marsili, E.. (2021). Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford., 389.
https://doi.org/10.1016/j.electacta.2021.138757

Olaifa K, Nikodinović-Runić J, Glišić B, Boschetto F, Marin E, Segreto F, Marsili E. Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing. in Electrochimica Acta. 2021;389.
doi:10.1016/j.electacta.2021.138757 .

Olaifa, Kayode, Nikodinović-Runić, Jasmina, Glišić, Biljana, Boschetto, Francesco, Marin, Elia, Segreto, Francesco, Marsili, Enrico, "Electroanalysis of Candida albicans biofilms: A suitable real-time tool for antifungal testing" in Electrochimica Acta, 389 (2021),
https://doi.org/10.1016/j.electacta.2021.138757 . .

TY  - JOUR
AU  - Savić, Nada D.
AU  - Petković, Branka B.
AU  - Vojnović, Sandra
AU  - Mojicević, Marija
AU  - Wadepohl, Hubert
AU  - Olaifa, Kayode
AU  - Marsili, Enrico
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1313
AB  - New dinuclear silver(i) complexes withN,N ',N '',N '''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), [Ag-2(NO3)(tpmc)]NO3 center dot 1.7H(2)O (1), [Ag-2(CF3SO3)(2)(tpmc)] (2), and [Ag-2(tpmc)](BF4)(2) (3) were synthesized and characterized by NMR (H-1 and(13)C), IR and UV- Vis spectroscopy, cyclic voltammetry and molar conductivity measurements. The molecular structures of the complexes were determined by single-crystal X-ray diffraction analysis. The spectroscopic and crystallographic data showed that the structure of the complexes strongly depends on the nature of the counteranion of silver(i) salt used for their synthesis. The antimicrobial activity of complexes1-3was examined against Gram-positive and Gram-negative bacteria and different species of unicellular fungus Candida spp. The ability of these complexes to inhibit the formation of Candida biofilms and to eradicate the already formed biofilms was tested in the standard microtiter plate-based assay. In addition, a bioelectrochemical testing of the antimicrobial activity of complex 1 against early biofilm was also performed. The obtained results indicated that complexes 1-3 showed increased activity toward Gram-negative bacteria and Candida spp. and could inhibit the formation of biofilms. In most cases, these complexes had positive selectivity indices and showed similar or even better activity with respect to the clinically used silver(i) sulfadiazine (AgSD). The values of the binding constants for complexes 1-3 to bovine serum albumin (BSA) were found to be high enough to indicate their binding to this biomolecule, but not so high as to prevent their release upon arrival at the target site. Moreover, the positive values of partition coefficients for these complexes indicated their ability to be transported through the cell membrane. Once inside the cell, complexes 1-3 could induce the formation of the reactive oxygen species (ROS) in C. albicanscells and/or interact with DNA. Taken together, silver(i) complexes with the tpmc ligand could be considered as novel antimicrobial compounds with favourable pharmacological properties, being safer than AgSD.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes
EP  - 10894
IS  - 31
SP  - 10880
VL  - 49
DO  - 10.1039/d0dt01272f
ER  - 

@article{
author = "Savić, Nada D. and Petković, Branka B. and Vojnović, Sandra and Mojicević, Marija and Wadepohl, Hubert and Olaifa, Kayode and Marsili, Enrico and Nikodinović-Runić, Jasmina and Djuran, Milos and Glišić, Biljana",
year = "2020",
abstract = "New dinuclear silver(i) complexes withN,N ',N '',N '''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), [Ag-2(NO3)(tpmc)]NO3 center dot 1.7H(2)O (1), [Ag-2(CF3SO3)(2)(tpmc)] (2), and [Ag-2(tpmc)](BF4)(2) (3) were synthesized and characterized by NMR (H-1 and(13)C), IR and UV- Vis spectroscopy, cyclic voltammetry and molar conductivity measurements. The molecular structures of the complexes were determined by single-crystal X-ray diffraction analysis. The spectroscopic and crystallographic data showed that the structure of the complexes strongly depends on the nature of the counteranion of silver(i) salt used for their synthesis. The antimicrobial activity of complexes1-3was examined against Gram-positive and Gram-negative bacteria and different species of unicellular fungus Candida spp. The ability of these complexes to inhibit the formation of Candida biofilms and to eradicate the already formed biofilms was tested in the standard microtiter plate-based assay. In addition, a bioelectrochemical testing of the antimicrobial activity of complex 1 against early biofilm was also performed. The obtained results indicated that complexes 1-3 showed increased activity toward Gram-negative bacteria and Candida spp. and could inhibit the formation of biofilms. In most cases, these complexes had positive selectivity indices and showed similar or even better activity with respect to the clinically used silver(i) sulfadiazine (AgSD). The values of the binding constants for complexes 1-3 to bovine serum albumin (BSA) were found to be high enough to indicate their binding to this biomolecule, but not so high as to prevent their release upon arrival at the target site. Moreover, the positive values of partition coefficients for these complexes indicated their ability to be transported through the cell membrane. Once inside the cell, complexes 1-3 could induce the formation of the reactive oxygen species (ROS) in C. albicanscells and/or interact with DNA. Taken together, silver(i) complexes with the tpmc ligand could be considered as novel antimicrobial compounds with favourable pharmacological properties, being safer than AgSD.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes",
pages = "10894-10880",
number = "31",
volume = "49",
doi = "10.1039/d0dt01272f"
}

Savić, N. D., Petković, B. B., Vojnović, S., Mojicević, M., Wadepohl, H., Olaifa, K., Marsili, E., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 49(31), 10880-10894.
https://doi.org/10.1039/d0dt01272f

Savić ND, Petković BB, Vojnović S, Mojicević M, Wadepohl H, Olaifa K, Marsili E, Nikodinović-Runić J, Djuran M, Glišić B. Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes. in Dalton Transactions. 2020;49(31):10880-10894.
doi:10.1039/d0dt01272f .

Savić, Nada D., Petković, Branka B., Vojnović, Sandra, Mojicević, Marija, Wadepohl, Hubert, Olaifa, Kayode, Marsili, Enrico, Nikodinović-Runić, Jasmina, Djuran, Milos, Glišić, Biljana, "Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes" in Dalton Transactions, 49, no. 31 (2020):10880-10894,
https://doi.org/10.1039/d0dt01272f . .