TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2159
AB  - Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Herbicide-resilient bacterial consortia in plant growth promotion
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2159
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Herbicide-resilient bacterial consortia in plant growth promotion",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2159"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159

Galić I, Bertani I, Bez C, Stanković N, Venturi V. Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "Herbicide-resilient bacterial consortia in plant growth promotion" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

TY  - CONF
AU  - Bez, Cristina
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2012
AB  - In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding
EP  - 71
SP  - 71
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2012
ER  - 

@conference{
author = "Bez, Cristina and Galić, Ivana and Bertani, Iris and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding",
pages = "71-71",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2012"
}

Bez, C., Galić, I., Bertani, I., Stanković, N.,& Venturi, V.. (2023). Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012

Bez C, Galić I, Bertani I, Stanković N, Venturi V. Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference. 2023;4:71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

Bez, Cristina, Galić, Ivana, Bertani, Iris, Stanković, Nada, Venturi, Vittorio, "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding" in 4th Belgrade Bioinformatics Conference, 4 (2023):71-71,
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://ceesme.ecolres.hu/CEESME2023_abstract_book_v1.pdf
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2163
AB  - organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.
C3  - CEESME Central and Eastern Europe Symposium on Microbial Ecology
T1  - High herbicide concentration tolerant bacteria in plant growth promotion
EP  - 98
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2163
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.",
journal = "CEESME Central and Eastern Europe Symposium on Microbial Ecology",
title = "High herbicide concentration tolerant bacteria in plant growth promotion",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2163"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology, 98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163

Galić I, Bertani I, Bez C, Stanković N, Venturi V. High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "High herbicide concentration tolerant bacteria in plant growth promotion" in CEESME Central and Eastern Europe Symposium on Microbial Ecology (2023):98-98,
https://hdl.handle.net/21.15107/rcub_imagine_2163 .