TY  - JOUR
AU  - Zimowska, Katarzyna
AU  - Filipović, Vuk
AU  - Nikodinović-Runić, Jasmina
AU  - Simić, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Zimowska, Malgorzata
AU  - Gurgul, Jacek
AU  - Ponjavić, Marijana
PY  - 2024
UR  - https://www.mdpi.com/2306-5354/11/8/847
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2493
AB  - The present study aimed to create a more sustainable and controlled delivery system based on natural biopolymer bacterial nanocellulose (BNC) and bacterial natural product actinomycin (Act), with the applicative potential in the biomedical field. In order to provide improved interaction between BNC and the active compound, and thus to modulate the release kinetics, the TEMPO oxidation of BNC support was carried out. A mix of actinomycins from bacterial fermentation (ActX) were used as natural antimicrobial agents with an established bioactivity profile and clinical use. BNC and TEMPO-oxidized BNC films with incorporated active compounds were obtained and analyzed by FTIR, SEM, XPS, and XRD. The ActX release profiles were determined in phosphate-buffer solution, PBS, at 37 °C over time. FTIR analysis confirmed the improved incorporation and efficiency of ActX adsorption on oxidized BNC due to the availability of more active sites provided by oxidation. SEM analysis indicated the incorporation of ActX into the less-dense morphology of the TEMPO-oxidized BNC in comparison to pure BNC. The release kinetics of ActX were significantly affected by the BNC structure, and the activated BNC sample indicated the sustained release of active compounds over time, corresponding to the Fickian diffusion mechanism. Antimicrobial tests using Staphylococcus aureus NCTC 6571 confirmed the potency of this BNC-based system for biomedical applications, taking advantage of the capacity of modified BNC to control and modulate the release of bioactive compounds.
PB  - MDPI
T2  - Bioengineering
T2  - Bioengineering
T1  - Modulating the Release Kinetics of Natural Product Actinomycin from Bacterial Nanocellulose Films and Their Antimicrobial Activity
IS  - 8
SP  - 847
VL  - 11
DO  - 10.3390/bioengineering11080847
ER  - 

@article{
author = "Zimowska, Katarzyna and Filipović, Vuk and Nikodinović-Runić, Jasmina and Simić, Jelena and Ilić-Tomić, Tatjana and Zimowska, Malgorzata and Gurgul, Jacek and Ponjavić, Marijana",
year = "2024",
abstract = "The present study aimed to create a more sustainable and controlled delivery system based on natural biopolymer bacterial nanocellulose (BNC) and bacterial natural product actinomycin (Act), with the applicative potential in the biomedical field. In order to provide improved interaction between BNC and the active compound, and thus to modulate the release kinetics, the TEMPO oxidation of BNC support was carried out. A mix of actinomycins from bacterial fermentation (ActX) were used as natural antimicrobial agents with an established bioactivity profile and clinical use. BNC and TEMPO-oxidized BNC films with incorporated active compounds were obtained and analyzed by FTIR, SEM, XPS, and XRD. The ActX release profiles were determined in phosphate-buffer solution, PBS, at 37 °C over time. FTIR analysis confirmed the improved incorporation and efficiency of ActX adsorption on oxidized BNC due to the availability of more active sites provided by oxidation. SEM analysis indicated the incorporation of ActX into the less-dense morphology of the TEMPO-oxidized BNC in comparison to pure BNC. The release kinetics of ActX were significantly affected by the BNC structure, and the activated BNC sample indicated the sustained release of active compounds over time, corresponding to the Fickian diffusion mechanism. Antimicrobial tests using Staphylococcus aureus NCTC 6571 confirmed the potency of this BNC-based system for biomedical applications, taking advantage of the capacity of modified BNC to control and modulate the release of bioactive compounds.",
publisher = "MDPI",
journal = "Bioengineering, Bioengineering",
title = "Modulating the Release Kinetics of Natural Product Actinomycin from Bacterial Nanocellulose Films and Their Antimicrobial Activity",
number = "8",
pages = "847",
volume = "11",
doi = "10.3390/bioengineering11080847"
}

Zimowska, K., Filipović, V., Nikodinović-Runić, J., Simić, J., Ilić-Tomić, T., Zimowska, M., Gurgul, J.,& Ponjavić, M.. (2024). Modulating the Release Kinetics of Natural Product Actinomycin from Bacterial Nanocellulose Films and Their Antimicrobial Activity. in Bioengineering
MDPI., 11(8), 847.
https://doi.org/10.3390/bioengineering11080847

Zimowska K, Filipović V, Nikodinović-Runić J, Simić J, Ilić-Tomić T, Zimowska M, Gurgul J, Ponjavić M. Modulating the Release Kinetics of Natural Product Actinomycin from Bacterial Nanocellulose Films and Their Antimicrobial Activity. in Bioengineering. 2024;11(8):847.
doi:10.3390/bioengineering11080847 .

Zimowska, Katarzyna, Filipović, Vuk, Nikodinović-Runić, Jasmina, Simić, Jelena, Ilić-Tomić, Tatjana, Zimowska, Malgorzata, Gurgul, Jacek, Ponjavić, Marijana, "Modulating the Release Kinetics of Natural Product Actinomycin from Bacterial Nanocellulose Films and Their Antimicrobial Activity" in Bioengineering, 11, no. 8 (2024):847,
https://doi.org/10.3390/bioengineering11080847 . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Filipović, Vuk
AU  - Pantelić, Lena
AU  - Milovanović, Jelena
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2024.1351583
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2387
AB  - Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.
PB  - Frontiers
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Late-stage diversification of bacterial natural products through biocatalysis
VL  - 12
DO  - 10.3389/fbioe.2024.1351583
ER  - 

@article{
author = "Lazić, Jelena and Filipović, Vuk and Pantelić, Lena and Milovanović, Jelena and Vojnović, Sandra and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.",
publisher = "Frontiers",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Late-stage diversification of bacterial natural products through biocatalysis",
volume = "12",
doi = "10.3389/fbioe.2024.1351583"
}

Lazić, J., Filipović, V., Pantelić, L., Milovanović, J., Vojnović, S.,& Nikodinović-Runić, J.. (2024). Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology
Frontiers., 12.
https://doi.org/10.3389/fbioe.2024.1351583

Lazić J, Filipović V, Pantelić L, Milovanović J, Vojnović S, Nikodinović-Runić J. Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology. 2024;12.
doi:10.3389/fbioe.2024.1351583 .

Lazić, Jelena, Filipović, Vuk, Pantelić, Lena, Milovanović, Jelena, Vojnović, Sandra, Nikodinović-Runić, Jasmina, "Late-stage diversification of bacterial natural products through biocatalysis" in Frontiers in Bioengineering and Biotechnology, 12 (2024),
https://doi.org/10.3389/fbioe.2024.1351583 . .

TY  - CONF
AU  - Filipović, Vuk
AU  - Ponjavić, Marijana
AU  - Lazić, Jelena
AU  - Milovanović, Jelena
AU  - Nikodinović- Runić, Jasmina
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2405
AB  - Bakterijski prirodni proizvodi (BPP) su od ključnog značaja u savremenoj medicini
zahvaljujući svojoj efikasnosti i širokoj kliničkoj primeni, a pored toga imaju veliki
potencijal i kao kandidati za razvoj novih lekova i za hemijske inovacije. Perspektiva
korišćenja biokatalize za diverzifikaciju BPP-a predstavlja privlačnu alternativu totalnoj
hemijskoj sintezi. Uprkos sve većem prihvatanju biokatalize kao ekološki prihvatljivog
pristupa, značajan deo sekvenciranih genoma, povezanih sa metaboličkim putevima za
biosintezu BNP-a i njihovim modifikacijskim enzimima, a kojima funkcija nije ispitana,
ostaje uglavnom neiskorišćen. Ova studija ističe ispitivanje zelenih biotransformacija 1-
hidroksifenazina i aktinomicina D, što može pomoći u pronalaženju novih strukturnih
optimizacija bioaktivnih molekula i unapređenju razvoja lekova.
AB  - Bacterial natural products (BNPs) are crucial in modern medicine due to their effectiveness
and diverse applications, as evidenced by their extensive clinical use, and also as potential
drug leads and for chemical innovation. The prospect of employing biocatalysis for latestage
diversification of BNPs presents an appealing alternative to total chemical synthesis.
Despite the growing acceptance of biocatalysis as an environmentally friendly approach, a
significant portion of orphan sequenced genomic data associated with metabolic pathways
for BNP biosynthesis and their modifying enzymes remains largely untapped. This study
highlights an examination of the green biotransformations to of 1-hydroxyphenazine and
actinomycin D, which may aid in identifying novel structural optimizations of bioactive
molecules and ultimately advancing drug development
PB  - Beograd : Srpsko hemijsko društvo
C3  - 60. Savetovanje Srpskog hemijskog društva
T1  - Zeleni pristup za diverzifikaciju bakterijskih prirodnih proizvoda
T1  - Green chemistry approaches for late-stage diversification of bacterial natural products
EP  - 125
SP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2405
ER  - 

@conference{
author = "Filipović, Vuk and Ponjavić, Marijana and Lazić, Jelena and Milovanović, Jelena and Nikodinović- Runić, Jasmina",
year = "2024",
abstract = "Bakterijski prirodni proizvodi (BPP) su od ključnog značaja u savremenoj medicini
zahvaljujući svojoj efikasnosti i širokoj kliničkoj primeni, a pored toga imaju veliki
potencijal i kao kandidati za razvoj novih lekova i za hemijske inovacije. Perspektiva
korišćenja biokatalize za diverzifikaciju BPP-a predstavlja privlačnu alternativu totalnoj
hemijskoj sintezi. Uprkos sve većem prihvatanju biokatalize kao ekološki prihvatljivog
pristupa, značajan deo sekvenciranih genoma, povezanih sa metaboličkim putevima za
biosintezu BNP-a i njihovim modifikacijskim enzimima, a kojima funkcija nije ispitana,
ostaje uglavnom neiskorišćen. Ova studija ističe ispitivanje zelenih biotransformacija 1-
hidroksifenazina i aktinomicina D, što može pomoći u pronalaženju novih strukturnih
optimizacija bioaktivnih molekula i unapređenju razvoja lekova., Bacterial natural products (BNPs) are crucial in modern medicine due to their effectiveness
and diverse applications, as evidenced by their extensive clinical use, and also as potential
drug leads and for chemical innovation. The prospect of employing biocatalysis for latestage
diversification of BNPs presents an appealing alternative to total chemical synthesis.
Despite the growing acceptance of biocatalysis as an environmentally friendly approach, a
significant portion of orphan sequenced genomic data associated with metabolic pathways
for BNP biosynthesis and their modifying enzymes remains largely untapped. This study
highlights an examination of the green biotransformations to of 1-hydroxyphenazine and
actinomycin D, which may aid in identifying novel structural optimizations of bioactive
molecules and ultimately advancing drug development",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "60. Savetovanje Srpskog hemijskog društva",
title = "Zeleni pristup za diverzifikaciju bakterijskih prirodnih proizvoda, Green chemistry approaches for late-stage diversification of bacterial natural products",
pages = "125-125",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2405"
}

Filipović, V., Ponjavić, M., Lazić, J., Milovanović, J.,& Nikodinović- Runić, J.. (2024). Zeleni pristup za diverzifikaciju bakterijskih prirodnih proizvoda. in 60. Savetovanje Srpskog hemijskog društva
Beograd : Srpsko hemijsko društvo., 125-125.
https://hdl.handle.net/21.15107/rcub_imagine_2405

Filipović V, Ponjavić M, Lazić J, Milovanović J, Nikodinović- Runić J. Zeleni pristup za diverzifikaciju bakterijskih prirodnih proizvoda. in 60. Savetovanje Srpskog hemijskog društva. 2024;:125-125.
https://hdl.handle.net/21.15107/rcub_imagine_2405 .

Filipović, Vuk, Ponjavić, Marijana, Lazić, Jelena, Milovanović, Jelena, Nikodinović- Runić, Jasmina, "Zeleni pristup za diverzifikaciju bakterijskih prirodnih proizvoda" in 60. Savetovanje Srpskog hemijskog društva (2024):125-125,
https://hdl.handle.net/21.15107/rcub_imagine_2405 .

TY  - JOUR
AU  - Babić Radić, Marija
AU  - Filipović, Vuk
AU  - Vuković, Jovana
AU  - Vukomanović, Marija
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2023
UR  - https://www.mdpi.com/2073-4360/15/7/1643
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1799
AB  - The idea of this study was to create a new scaffolding system based on 2-hydroxyethyl methacrylate, gelatin, and alginate that contains titanium(IV) oxide nanoparticles as a platform for the controlled release of the bioactive agent curcumin. The innovative strategy to develop hybrid scaffolds was the modified porogenation method. The effect of the scaffold composition on the chemical, morphology, porosity, mechanical, hydrophilicity, swelling, degradation, biocompatibility, loading, and release features of hybrid scaffolds was evaluated. A porous structure with interconnected pores in the range of 52.33–65.76%, favorable swelling capacity, fully hydrophilic surfaces, degradability to 45% for 6 months, curcumin loading efficiency above 96%, and favorable controlled release profiles were obtained. By applying four kinetic models of release, valuable parameters were obtained for the curcumin/PHEMA/gelatin/alginate/TiO2 release platform. Cytotoxicity test results depend on the composition of the scaffolds and showed satisfactory cell growth with visible cell accumulation on the hybrid surfaces. The constructed hybrid scaffolds have suitable high-performance properties, suggesting potential for further in vivo and clinical studies.
T2  - Polymers
T2  - Polymers
T1  - 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform
IS  - 7
SP  - 1643
VL  - 15
DO  - 10.3390/polym15071643
ER  - 

@article{
author = "Babić Radić, Marija and Filipović, Vuk and Vuković, Jovana and Vukomanović, Marija and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2023",
abstract = "The idea of this study was to create a new scaffolding system based on 2-hydroxyethyl methacrylate, gelatin, and alginate that contains titanium(IV) oxide nanoparticles as a platform for the controlled release of the bioactive agent curcumin. The innovative strategy to develop hybrid scaffolds was the modified porogenation method. The effect of the scaffold composition on the chemical, morphology, porosity, mechanical, hydrophilicity, swelling, degradation, biocompatibility, loading, and release features of hybrid scaffolds was evaluated. A porous structure with interconnected pores in the range of 52.33–65.76%, favorable swelling capacity, fully hydrophilic surfaces, degradability to 45% for 6 months, curcumin loading efficiency above 96%, and favorable controlled release profiles were obtained. By applying four kinetic models of release, valuable parameters were obtained for the curcumin/PHEMA/gelatin/alginate/TiO2 release platform. Cytotoxicity test results depend on the composition of the scaffolds and showed satisfactory cell growth with visible cell accumulation on the hybrid surfaces. The constructed hybrid scaffolds have suitable high-performance properties, suggesting potential for further in vivo and clinical studies.",
journal = "Polymers, Polymers",
title = "2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform",
number = "7",
pages = "1643",
volume = "15",
doi = "10.3390/polym15071643"
}

Babić Radić, M., Filipović, V., Vuković, J., Vukomanović, M., Ilić-Tomić, T., Nikodinović-Runić, J.,& Tomić, S.. (2023). 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform. in Polymers, 15(7), 1643.
https://doi.org/10.3390/polym15071643

Babić Radić M, Filipović V, Vuković J, Vukomanović M, Ilić-Tomić T, Nikodinović-Runić J, Tomić S. 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform. in Polymers. 2023;15(7):1643.
doi:10.3390/polym15071643 .

Babić Radić, Marija, Filipović, Vuk, Vuković, Jovana, Vukomanović, Marija, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, Tomić, Simonida, "2-Hydroxyethyl Methacrylate/Gelatin/Alginate Scaffolds Reinforced with Nano TiO2 as a Promising Curcumin Release Platform" in Polymers, 15, no. 7 (2023):1643,
https://doi.org/10.3390/polym15071643 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Vukašin
AU  - Filipović, Vuk
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1915
AB  - The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365
EP  - 168
SP  - 167
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1915
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Vukašin and Filipović, Vuk and Ilić-Tomić, Tatjana and Vojnović, Sandra",
year = "2023",
abstract = "The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365",
pages = "168-167",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1915"
}

Stevanović, M., Janković, V., Filipović, V., Ilić-Tomić, T.,& Vojnović, S.. (2023). Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915

Stevanović M, Janković V, Filipović V, Ilić-Tomić T, Vojnović S. Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

Stevanović, Milena, Janković, Vukašin, Filipović, Vuk, Ilić-Tomić, Tatjana, Vojnović, Sandra, "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):167-168,
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Vuković, Jovana
AU  - Babić Radić, Marija
AU  - Filipović, Vuk
AU  - Živanović, Dubravka
AU  - Nikolić, Miloš
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4360/15/3/589
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1788
AB  - Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.
PB  - MDPI
T2  - Polymers
T2  - Polymers
T1  - Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration
IS  - 3
SP  - 589
VL  - 15
DO  - 10.3390/polym15030589
ER  - 

@article{
author = "Tomić, Simonida and Vuković, Jovana and Babić Radić, Marija and Filipović, Vuk and Živanović, Dubravka and Nikolić, Miloš and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.",
publisher = "MDPI",
journal = "Polymers, Polymers",
title = "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration",
number = "3",
pages = "589",
volume = "15",
doi = "10.3390/polym15030589"
}

Tomić, S., Vuković, J., Babić Radić, M., Filipović, V., Živanović, D., Nikolić, M.,& Nikodinović-Runić, J.. (2023). Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers
MDPI., 15(3), 589.
https://doi.org/10.3390/polym15030589

Tomić S, Vuković J, Babić Radić M, Filipović V, Živanović D, Nikolić M, Nikodinović-Runić J. Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers. 2023;15(3):589.
doi:10.3390/polym15030589 .

Tomić, Simonida, Vuković, Jovana, Babić Radić, Marija, Filipović, Vuk, Živanović, Dubravka, Nikolić, Miloš, Nikodinović-Runić, Jasmina, "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration" in Polymers, 15, no. 3 (2023):589,
https://doi.org/10.3390/polym15030589 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Filipović, Vuk
AU  - Topakas, Evangelos
AU  - Karnaouri, Anthi
AU  - Živković, Jelena
AU  - Krgović, Nemanja
AU  - Mudrić, Jelena
AU  - Savikin, Katarina
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/16/2995
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2025
AB  - Background: Bacterial nanocellulose (BNC) has gained in popularity over the years due to its outstanding properties such as renewability, biocompatibility, and bioavailability, and its use as an eco-friendly material of the future for replacing petrochemical products. (2) Methods: This research refers to the utilization of lignocellulose coming from wood waste via enzymatic hydrolysis to produce biopolymer BNC with an accumulation rate of 0.09 mg/mL/day. Besides its significant contribution to the sustainability, circularity, and valorization of biomass products, the obtained BNC was functionalized through the adsorption of black raspberry extract (BR) by simple soaking. (3) Results: BR contained 77.25 ± 0.23 mg GAE/g of total phenolics and 27.42 ± 0.32 mg CGE/g of total anthocyanins. The antioxidant and antimicrobial activity of BR was evaluated by DPPH (60.51 ± 0.18 µg/mL) and FRAP (1.66 ± 0.03 mmol Fe2+/g) and using a standard disc diffusion assay, respectively. The successful synthesis and interactions between BNC and BR were confirmed by FTIR analysis, while the morphology of the new nutrient-enriched material was investigated by SEM analysis. Moreover, the in vitro release kinetics of a main active compound (cyanidin-3-O-rutinoside) was tested in different release media. (4) Conclusions: The upcycling process of lignocellulose into enriched BNC has been demonstrated. All findings emphasize the potential of BNC–BR as a sustainable food industry material.
T2  - Foods
T2  - Foods
T1  - Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient
IS  - 16
SP  - 2995
VL  - 12
DO  - 10.3390/foods12162995
ER  - 

@article{
author = "Ponjavić, Marijana and Filipović, Vuk and Topakas, Evangelos and Karnaouri, Anthi and Živković, Jelena and Krgović, Nemanja and Mudrić, Jelena and Savikin, Katarina and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Background: Bacterial nanocellulose (BNC) has gained in popularity over the years due to its outstanding properties such as renewability, biocompatibility, and bioavailability, and its use as an eco-friendly material of the future for replacing petrochemical products. (2) Methods: This research refers to the utilization of lignocellulose coming from wood waste via enzymatic hydrolysis to produce biopolymer BNC with an accumulation rate of 0.09 mg/mL/day. Besides its significant contribution to the sustainability, circularity, and valorization of biomass products, the obtained BNC was functionalized through the adsorption of black raspberry extract (BR) by simple soaking. (3) Results: BR contained 77.25 ± 0.23 mg GAE/g of total phenolics and 27.42 ± 0.32 mg CGE/g of total anthocyanins. The antioxidant and antimicrobial activity of BR was evaluated by DPPH (60.51 ± 0.18 µg/mL) and FRAP (1.66 ± 0.03 mmol Fe2+/g) and using a standard disc diffusion assay, respectively. The successful synthesis and interactions between BNC and BR were confirmed by FTIR analysis, while the morphology of the new nutrient-enriched material was investigated by SEM analysis. Moreover, the in vitro release kinetics of a main active compound (cyanidin-3-O-rutinoside) was tested in different release media. (4) Conclusions: The upcycling process of lignocellulose into enriched BNC has been demonstrated. All findings emphasize the potential of BNC–BR as a sustainable food industry material.",
journal = "Foods, Foods",
title = "Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient",
number = "16",
pages = "2995",
volume = "12",
doi = "10.3390/foods12162995"
}

Ponjavić, M., Filipović, V., Topakas, E., Karnaouri, A., Živković, J., Krgović, N., Mudrić, J., Savikin, K.,& Nikodinović-Runić, J.. (2023). Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient. in Foods, 12(16), 2995.
https://doi.org/10.3390/foods12162995

Ponjavić M, Filipović V, Topakas E, Karnaouri A, Živković J, Krgović N, Mudrić J, Savikin K, Nikodinović-Runić J. Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient. in Foods. 2023;12(16):2995.
doi:10.3390/foods12162995 .

Ponjavić, Marijana, Filipović, Vuk, Topakas, Evangelos, Karnaouri, Anthi, Živković, Jelena, Krgović, Nemanja, Mudrić, Jelena, Savikin, Katarina, Nikodinović-Runić, Jasmina, "Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient" in Foods, 12, no. 16 (2023):2995,
https://doi.org/10.3390/foods12162995 . .

TY  - JOUR
AU  - Vuković, Jovana S.
AU  - Filipović, Vuk V.
AU  - Babić Radić, Marija M.
AU  - Vukomanović, Marija
AU  - Milivojević, Dušan
AU  - Ilic-Tomic, Tatjana
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1669
AB  - Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.
T2  - Polymers
T2  - Polymers
T1  - In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds
IS  - 20
SP  - 4459
VL  - 14
DO  - 10.3390/polym14204459
ER  - 

@article{
author = "Vuković, Jovana S. and Filipović, Vuk V. and Babić Radić, Marija M. and Vukomanović, Marija and Milivojević, Dušan and Ilic-Tomic, Tatjana and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj",
year = "2022",
abstract = "Scaffold hydrogel biomaterials designed to have advantageous biofunctional properties, which can be applied for controlled bioactive agent release, represent an important concept in biomedical tissue engineering. Our goal was to create scaffolding materials that mimic living tissue for biomedical utilization. In this study, two novel series of interpenetrating hydrogel networks (IPNs) based on 2-hydroxyethyl methacrylate/gelatin and 2-hydroxyethyl methacrylate/alginate were crosslinked using N-ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Characterization included examining the effects of crosslinker type and concentration on structure, morphological and mechanical properties, in vitro swelling, hydrophilicity as well as on the in vitro cell viability (fibroblast cells) and in vivo (Caenorhabditis elegans) interactions of novel biomaterials. The engineered IPN hydrogel scaffolds show an interconnected pore morphology and porosity range of 62.36 to 85.20%, favorable in vitro swelling capacity, full hydrophilicity, and Young’s modulus values in the range of 1.40 to 7.50 MPa. In vitro assay on healthy human fibroblast (MRC5 cells) by MTT test and in vivo (Caenorhabditis elegans) survival assays show the advantageous biocompatible properties of novel IPN hydrogel scaffolds. Furthermore, in vitro controlled release study of the therapeutic agent resveratrol showed that these novel scaffolding systems are suitable controlled release platforms. The results revealed that the use of EDC and the combination of EDC/NHS crosslinkers can be applied to prepare and tune the properties of the IPN 2-hydroxyethyl methacrylate/alginate and 2-hydroxyethyl methacrylate/gelatin hydrogel scaffolds series, which have shown great potential for biomedical engineering applications.",
journal = "Polymers, Polymers",
title = "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds",
number = "20",
pages = "4459",
volume = "14",
doi = "10.3390/polym14204459"
}

Vuković, J. S., Filipović, V. V., Babić Radić, M. M., Vukomanović, M., Milivojević, D., Ilic-Tomic, T., Nikodinović-Runić, J.,& Tomić, S. L.. (2022). In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers, 14(20), 4459.
https://doi.org/10.3390/polym14204459

Vuković JS, Filipović VV, Babić Radić MM, Vukomanović M, Milivojević D, Ilic-Tomic T, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds. in Polymers. 2022;14(20):4459.
doi:10.3390/polym14204459 .

Vuković, Jovana S., Filipović, Vuk V., Babić Radić, Marija M., Vukomanović, Marija, Milivojević, Dušan, Ilic-Tomic, Tatjana, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj, "In Vitro and In Vivo Biocompatible and Controlled Resveratrol Release Performances of HEMA/Alginate and HEMA/Gelatin IPN Hydrogel Scaffolds" in Polymers, 14, no. 20 (2022):4459,
https://doi.org/10.3390/polym14204459 . .

TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Filipović, Vuk V.
AU  - Vukomanović, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1530
AB  - The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.
PB  - MDPI, Basel
T2  - Gels
T1  - Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials
IS  - 1
VL  - 8
DO  - 10.3390/gels8010022
ER  - 

@article{
author = "Babić Radić, Marija M. and Filipović, Vuk V. and Vukomanović, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj.",
year = "2022",
abstract = "The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.",
publisher = "MDPI, Basel",
journal = "Gels",
title = "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials",
number = "1",
volume = "8",
doi = "10.3390/gels8010022"
}

Babić Radić, M. M., Filipović, V. V., Vukomanović, M., Nikodinović-Runić, J.,& Tomić, S. Lj.. (2022). Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels
MDPI, Basel., 8(1).
https://doi.org/10.3390/gels8010022

Babić Radić MM, Filipović VV, Vukomanović M, Nikodinović-Runić J, Tomić SL. Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels. 2022;8(1).
doi:10.3390/gels8010022 .

Babić Radić, Marija M., Filipović, Vuk V., Vukomanović, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj., "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials" in Gels, 8, no. 1 (2022),
https://doi.org/10.3390/gels8010022 . .

TY  - JOUR
AU  - Filipović, Vuk V.
AU  - Babić, Marija M.
AU  - Godevac, Dejan
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1256
AB  - New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Macromolecular Chemistry and Physics
T1  - In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release
IS  - 17
VL  - 220
DO  - 10.1002/macp.201900188
ER  - 

@article{
author = "Filipović, Vuk V. and Babić, Marija M. and Godevac, Dejan and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj",
year = "2019",
abstract = "New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Macromolecular Chemistry and Physics",
title = "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release",
number = "17",
volume = "220",
doi = "10.1002/macp.201900188"
}

Filipović, V. V., Babić, M. M., Godevac, D., Pavić, A., Nikodinović-Runić, J.,& Tomić, S. L.. (2019). In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics
Wiley-V C H Verlag Gmbh, Weinheim., 220(17).
https://doi.org/10.1002/macp.201900188

Filipović VV, Babić MM, Godevac D, Pavić A, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics. 2019;220(17).
doi:10.1002/macp.201900188 .

Filipović, Vuk V., Babić, Marija M., Godevac, Dejan, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj, "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release" in Macromolecular Chemistry and Physics, 220, no. 17 (2019),
https://doi.org/10.1002/macp.201900188 . .