TY  - JOUR
AU  - Hertrampf, Gesa
AU  - Vojnović, Sandra
AU  - Müller, Jonas I.
AU  - Merten, Christian
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://doi.org/10.1021/acs.joc.3c01285
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2153
AB  - The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.
T2  - The Journal of Organic Chemistry
T1  - Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A
EP  - 14188
IS  - 19
SP  - 14184
VL  - 88
DO  - 10.1021/acs.joc.3c01285
ER  - 

@article{
author = "Hertrampf, Gesa and Vojnović, Sandra and Müller, Jonas I. and Merten, Christian and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.",
journal = "The Journal of Organic Chemistry",
title = "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A",
pages = "14188-14184",
number = "19",
volume = "88",
doi = "10.1021/acs.joc.3c01285"
}

Hertrampf, G., Vojnović, S., Müller, J. I., Merten, C., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry, 88(19), 14184-14188.
https://doi.org/10.1021/acs.joc.3c01285

Hertrampf G, Vojnović S, Müller JI, Merten C, Nikodinović-Runić J, Gulder TAM. Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry. 2023;88(19):14184-14188.
doi:10.1021/acs.joc.3c01285 .

Hertrampf, Gesa, Vojnović, Sandra, Müller, Jonas I., Merten, Christian, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A" in The Journal of Organic Chemistry, 88, no. 19 (2023):14184-14188,
https://doi.org/10.1021/acs.joc.3c01285 . .

TY  - JOUR
AU  - Milzarek, Tobias M.
AU  - Stevanović, Milena
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Iliasov, Denis
AU  - Wolf, Diana
AU  - Mascher, Thorsten
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2077
AB  - The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.
T2  - ACS Infectious Diseases
T2  - ACS Infectious DiseasesACS Infect. Dis.
T1  - Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs
DO  - 10.1021/acsinfecdis.3c00232
ER  - 

@article{
author = "Milzarek, Tobias M. and Stevanović, Milena and Milivojević, Dušan and Vojnović, Sandra and Iliasov, Denis and Wolf, Diana and Mascher, Thorsten and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.",
journal = "ACS Infectious Diseases, ACS Infectious DiseasesACS Infect. Dis.",
title = "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs",
doi = "10.1021/acsinfecdis.3c00232"
}

Milzarek, T. M., Stevanović, M., Milivojević, D., Vojnović, S., Iliasov, D., Wolf, D., Mascher, T., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases.
https://doi.org/10.1021/acsinfecdis.3c00232

Milzarek TM, Stevanović M, Milivojević D, Vojnović S, Iliasov D, Wolf D, Mascher T, Nikodinović-Runić J, Gulder TAM. Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases. 2023;.
doi:10.1021/acsinfecdis.3c00232 .

Milzarek, Tobias M., Stevanović, Milena, Milivojević, Dušan, Vojnović, Sandra, Iliasov, Denis, Wolf, Diana, Mascher, Thorsten, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs" in ACS Infectious Diseases (2023),
https://doi.org/10.1021/acsinfecdis.3c00232 . .

TY  - JOUR
AU  - Hertrampf, Gesa
AU  - Kusserow, Kalina
AU  - Vojnović, Sandra
AU  - Pavić, Aleksandar
AU  - Mueller, Jonas, I
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1553
AB  - The increasing emergence of resistances against established antibiotics is a substantial threat to human health. The discovery of new compounds with potent antibiotic activity is thus of utmost importance. Within this work, we identify strong antibiotic activity of the natural product myxocoumarin B from Stigmatella aurantiaca MYX-030 against a range of clinically relevant bacterial pathogens, including clinical isolates of MRSA. A focused library of structural analogs was synthesized to explore initial structure-activity relationships and to identify equipotent myxocoumarin derivatives devoid of the natural nitro substituent to significantly streamline synthetic access. The cytotoxicity of the myxocoumarins as well as their potential to cure bacterial infections in vivo was established using a zebrafish model system. Our results reveal the exceptional antibiotic activity of the myxocoumarin scaffold and hence its potential for the development of novel antibiotics.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry-A European Journal
T1  - Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo
IS  - 32
VL  - 28
DO  - 10.1002/chem.202200394
ER  - 

@article{
author = "Hertrampf, Gesa and Kusserow, Kalina and Vojnović, Sandra and Pavić, Aleksandar and Mueller, Jonas, I and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2022",
abstract = "The increasing emergence of resistances against established antibiotics is a substantial threat to human health. The discovery of new compounds with potent antibiotic activity is thus of utmost importance. Within this work, we identify strong antibiotic activity of the natural product myxocoumarin B from Stigmatella aurantiaca MYX-030 against a range of clinically relevant bacterial pathogens, including clinical isolates of MRSA. A focused library of structural analogs was synthesized to explore initial structure-activity relationships and to identify equipotent myxocoumarin derivatives devoid of the natural nitro substituent to significantly streamline synthetic access. The cytotoxicity of the myxocoumarins as well as their potential to cure bacterial infections in vivo was established using a zebrafish model system. Our results reveal the exceptional antibiotic activity of the myxocoumarin scaffold and hence its potential for the development of novel antibiotics.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry-A European Journal",
title = "Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo",
number = "32",
volume = "28",
doi = "10.1002/chem.202200394"
}

Hertrampf, G., Kusserow, K., Vojnović, S., Pavić, A., Mueller, J. I., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2022). Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo. in Chemistry-A European Journal
Wiley-V C H Verlag Gmbh, Weinheim., 28(32).
https://doi.org/10.1002/chem.202200394

Hertrampf G, Kusserow K, Vojnović S, Pavić A, Mueller JI, Nikodinović-Runić J, Gulder TAM. Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo. in Chemistry-A European Journal. 2022;28(32).
doi:10.1002/chem.202200394 .

Hertrampf, Gesa, Kusserow, Kalina, Vojnović, Sandra, Pavić, Aleksandar, Mueller, Jonas, I, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo" in Chemistry-A European Journal, 28, no. 32 (2022),
https://doi.org/10.1002/chem.202200394 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - D'Agostino, Paul M.
AU  - Mojicević, Marija
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1527
AB  - Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production
EP  - 2568
IS  - 4
SP  - 2560
VL  - 133
DO  - 10.1111/jam.15726
ER  - 

@article{
author = "Stevanović, Milena and D'Agostino, Paul M. and Mojicević, Marija and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2022",
abstract = "Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production",
pages = "2568-2560",
number = "4",
volume = "133",
doi = "10.1111/jam.15726"
}

Stevanović, M., D'Agostino, P. M., Mojicević, M., Gulder, T. A. M., Nikodinović-Runić, J.,& Vojnović, S.. (2022). Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology
Wiley, Hoboken., 133(4), 2560-2568.
https://doi.org/10.1111/jam.15726

Stevanović M, D'Agostino PM, Mojicević M, Gulder TAM, Nikodinović-Runić J, Vojnović S. Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology. 2022;133(4):2560-2568.
doi:10.1111/jam.15726 .

Stevanović, Milena, D'Agostino, Paul M., Mojicević, Marija, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production" in Journal of Applied Microbiology, 133, no. 4 (2022):2560-2568,
https://doi.org/10.1111/jam.15726 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Senthamaraikannan, Ramsankar
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1384
AB  - Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.
PB  - Wiley, Hoboken
T2  - Microbiologyopen
T1  - Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
IS  - 3
VL  - 9
DO  - 10.1002/mbo3.986
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Pavić, Aleksandar and Vojnović, Sandra and Senthamaraikannan, Ramsankar and Vasiljević, Branka and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.",
publisher = "Wiley, Hoboken",
journal = "Microbiologyopen",
title = "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties",
number = "3",
volume = "9",
doi = "10.1002/mbo3.986"
}

Mojicević, M., D'Agostino, P. M., Pavić, A., Vojnović, S., Senthamaraikannan, R., Vasiljević, B., Gulder, T. A. M.,& Nikodinović-Runić, J.. (2020). Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen
Wiley, Hoboken., 9(3).
https://doi.org/10.1002/mbo3.986

Mojicević M, D'Agostino PM, Pavić A, Vojnović S, Senthamaraikannan R, Vasiljević B, Gulder TAM, Nikodinović-Runić J. Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen. 2020;9(3).
doi:10.1002/mbo3.986 .

Mojicević, Marija, D'Agostino, Paul M., Pavić, Aleksandar, Vojnović, Sandra, Senthamaraikannan, Ramsankar, Vasiljević, Branka, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties" in Microbiologyopen, 9, no. 3 (2020),
https://doi.org/10.1002/mbo3.986 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Vojnović, Sandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1212
AB  - The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.
PB  - Springer, New York
T2  - International Microbiology
T1  - Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)
EP  - 353
IS  - 3
SP  - 343
VL  - 22
DO  - 10.1007/s10123-019-00054-8
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Nikodinović-Runić, Jasmina and Vasiljević, Branka and Gulder, Tobias A. M. and Vojnović, Sandra",
year = "2019",
abstract = "The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.",
publisher = "Springer, New York",
journal = "International Microbiology",
title = "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)",
pages = "353-343",
number = "3",
volume = "22",
doi = "10.1007/s10123-019-00054-8"
}

Mojicević, M., D'Agostino, P. M., Nikodinović-Runić, J., Vasiljević, B., Gulder, T. A. M.,& Vojnović, S.. (2019). Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology
Springer, New York., 22(3), 343-353.
https://doi.org/10.1007/s10123-019-00054-8

Mojicević M, D'Agostino PM, Nikodinović-Runić J, Vasiljević B, Gulder TAM, Vojnović S. Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology. 2019;22(3):343-353.
doi:10.1007/s10123-019-00054-8 .

Mojicević, Marija, D'Agostino, Paul M., Nikodinović-Runić, Jasmina, Vasiljević, Branka, Gulder, Tobias A. M., Vojnović, Sandra, "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)" in International Microbiology, 22, no. 3 (2019):343-353,
https://doi.org/10.1007/s10123-019-00054-8 . .

TY  - JOUR
AU  - Mueller, Jonas I.
AU  - Kusserow, Kalina
AU  - Hertrampf, Gesa
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1290
AB  - The myxocoumarins A and B from Stigmatella aurantiaca MYX-030 are natural products featuring unusual nitro-and long-chain alkyl substitution. While myxocoumarin A was shown to exhibit strong antifungal properties, the antifungal potential of myxocoumarin B was not yet assessed due to low production titers during initial isolation. We therefore developed a total synthesis of myxocoumarin B that involves a late-stage Pd-catalyzed nitration of the coumarin core. The availability of synthetic material facilitated the initial evaluation of the bioactivity of myxocoumarin B, which revealed a lack of activity against medically relevant Candida sp. and low cytotoxicity in vitro against human fibroblasts (MRC-5) and in vivo (zebrafish).
PB  - Royal Soc Chemistry, Cambridge
T2  - Organic & Biomolecular Chemistry
T1  - Synthesis and initial biological evaluation of myxocoumarin B
EP  - 1969
IS  - 7
SP  - 1966
VL  - 17
DO  - 10.1039/c8ob02273a
ER  - 

@article{
author = "Mueller, Jonas I. and Kusserow, Kalina and Hertrampf, Gesa and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2019",
abstract = "The myxocoumarins A and B from Stigmatella aurantiaca MYX-030 are natural products featuring unusual nitro-and long-chain alkyl substitution. While myxocoumarin A was shown to exhibit strong antifungal properties, the antifungal potential of myxocoumarin B was not yet assessed due to low production titers during initial isolation. We therefore developed a total synthesis of myxocoumarin B that involves a late-stage Pd-catalyzed nitration of the coumarin core. The availability of synthetic material facilitated the initial evaluation of the bioactivity of myxocoumarin B, which revealed a lack of activity against medically relevant Candida sp. and low cytotoxicity in vitro against human fibroblasts (MRC-5) and in vivo (zebrafish).",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Organic & Biomolecular Chemistry",
title = "Synthesis and initial biological evaluation of myxocoumarin B",
pages = "1969-1966",
number = "7",
volume = "17",
doi = "10.1039/c8ob02273a"
}

Mueller, J. I., Kusserow, K., Hertrampf, G., Pavić, A., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2019). Synthesis and initial biological evaluation of myxocoumarin B. in Organic & Biomolecular Chemistry
Royal Soc Chemistry, Cambridge., 17(7), 1966-1969.
https://doi.org/10.1039/c8ob02273a

Mueller JI, Kusserow K, Hertrampf G, Pavić A, Nikodinović-Runić J, Gulder TAM. Synthesis and initial biological evaluation of myxocoumarin B. in Organic & Biomolecular Chemistry. 2019;17(7):1966-1969.
doi:10.1039/c8ob02273a .

Mueller, Jonas I., Kusserow, Kalina, Hertrampf, Gesa, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Synthesis and initial biological evaluation of myxocoumarin B" in Organic & Biomolecular Chemistry, 17, no. 7 (2019):1966-1969,
https://doi.org/10.1039/c8ob02273a . .