TY  - JOUR
AU  - Karagüzel, Ayşe
AU  - Uğur, Sümeyye Buran
AU  - Çetinkaya, Yasin
AU  - Doğan, Şengül Dilem
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
AU  - Gündüz, Miyase Gözde
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0022286024003107
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2317
AB  - Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.
PB  - Elsevier
T2  - Journal of Molecular Structure
T2  - Journal of Molecular StructureJournal of Molecular Structure
T1  - Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity
SP  - 137787
DO  - 10.1016/j.molstruc.2024.137787
ER  - 

@article{
author = "Karagüzel, Ayşe and Uğur, Sümeyye Buran and Çetinkaya, Yasin and Doğan, Şengül Dilem and Stevanović, Milena and Nikodinović-Runić, Jasmina and Gündüz, Miyase Gözde",
year = "2024",
abstract = "Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure, Journal of Molecular StructureJournal of Molecular Structure",
title = "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity",
pages = "137787",
doi = "10.1016/j.molstruc.2024.137787"
}

Karagüzel, A., Uğur, S. B., Çetinkaya, Y., Doğan, Ş. D., Stevanović, M., Nikodinović-Runić, J.,& Gündüz, M. G.. (2024). Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure
Elsevier., 137787.
https://doi.org/10.1016/j.molstruc.2024.137787

Karagüzel A, Uğur SB, Çetinkaya Y, Doğan ŞD, Stevanović M, Nikodinović-Runić J, Gündüz MG. Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure. 2024;:137787.
doi:10.1016/j.molstruc.2024.137787 .

Karagüzel, Ayşe, Uğur, Sümeyye Buran, Çetinkaya, Yasin, Doğan, Şengül Dilem, Stevanović, Milena, Nikodinović-Runić, Jasmina, Gündüz, Miyase Gözde, "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity" in Journal of Molecular Structure (2024):137787,
https://doi.org/10.1016/j.molstruc.2024.137787 . .

TY  - JOUR
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Ilić-Tomić, Tatjana
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://doi.org/10.1007/s00253-023-12900-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2314
AB  - The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.
PB  - Springer Nature
T2  - Applied Microbiology and Biotechnology
T1  - Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
IS  - 1
SP  - 185
VL  - 108
DO  - 10.1007/s00253-023-12900-x
ER  - 

@article{
author = "Vojnović, Sandra and Aleksić, Ivana and Ilić-Tomić, Tatjana and Stevanović, Milena and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.",
publisher = "Springer Nature",
journal = "Applied Microbiology and Biotechnology",
title = "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes",
number = "1",
pages = "185",
volume = "108",
doi = "10.1007/s00253-023-12900-x"
}

Vojnović, S., Aleksić, I., Ilić-Tomić, T., Stevanović, M.,& Nikodinović-Runić, J.. (2024). Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology
Springer Nature., 108(1), 185.
https://doi.org/10.1007/s00253-023-12900-x

Vojnović S, Aleksić I, Ilić-Tomić T, Stevanović M, Nikodinović-Runić J. Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology. 2024;108(1):185.
doi:10.1007/s00253-023-12900-x .

Vojnović, Sandra, Aleksić, Ivana, Ilić-Tomić, Tatjana, Stevanović, Milena, Nikodinović-Runić, Jasmina, "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes" in Applied Microbiology and Biotechnology, 108, no. 1 (2024):185,
https://doi.org/10.1007/s00253-023-12900-x . .

TY  - JOUR
AU  - Özcan, Esma
AU  - Vagolu, Siva Krishna
AU  - Gündüz, Miyase Gözde
AU  - Stevanović, Milena
AU  - Kökbudak, Zülbiye
AU  - Tønjum, Tone
AU  - Nikodinović-Runić, Jasmina
AU  - Çetinkaya, Yasin
AU  - Doğan, Şengül Dilem
PY  - 2023
UR  - https://doi.org/10.1021/acsomega.3c03018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2154
AB  - The discovery of new antimicrobial agents as a means of treating drug-resistant microbial pathogens is of utmost significance to overcome their immense risk to human well-being. The current investigation involves the development, synthesis, and assessment of the antimicrobial efficacy of novel quinoline derivatives incorporating a thiosemicarbazide functionality. To design the target compounds (QST1–QST14), we applied the molecular hybridization approach to link various thiosemicarbazides to the quinoline core with a sulfonyl group. Upon the synthesis and completion of structural characterization via spectroscopic techniques (1H NMR, 13C NMR, 15N NMR, IR, and HRMS), the title molecules were extensively evaluated for their potential antitubercular, antibacterial, and antifungal activities. N-(3-Chlorophenyl)-2-(quinolin-8-ylsulfonyl)hydrazine-1-carbothioamide (QST4), the most effective compound against Mycobacterium tuberculosis H37Rv, was also tested on isoniazid-resistant clinical isolates with katG and inhA promoter mutations. Based on molecular docking studies, QST4 was also likely to demonstrate its antimycobacterial activity through inhibition of the InhA enzyme. Furthermore, three derivatives (QST3, QST4, and QST10) with preferable antimicrobial and drug-like profiles were also shown to be nontoxic against human embryonic kidney (HEK) cells. All compounds were optimized by the density functional theory method using B3LYP with the 6-31+G(d,p) basis set. Structural analysis, natural bond orbital calculations of donor–acceptor interactions, molecular electrostatic potential analysis, and frontier molecular orbital analysis were carried out. Quantum chemical descriptors and charges on the atoms were determined to compare the strengths of the intramolecular hydrogen bonds formed and their stabilities. We determined that the sulfur atom forms a stronger intramolecular hydrogen bond than the nitrogen, oxygen, and fluorine atoms in these sulfonyl thiosemicarbazide derivatives.
T2  - ACS Omega
T1  - Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities
EP  - 40152
IS  - 43
SP  - 40140
VL  - 8
DO  - 10.1021/acsomega.3c03018
ER  - 

@article{
author = "Özcan, Esma and Vagolu, Siva Krishna and Gündüz, Miyase Gözde and Stevanović, Milena and Kökbudak, Zülbiye and Tønjum, Tone and Nikodinović-Runić, Jasmina and Çetinkaya, Yasin and Doğan, Şengül Dilem",
year = "2023",
abstract = "The discovery of new antimicrobial agents as a means of treating drug-resistant microbial pathogens is of utmost significance to overcome their immense risk to human well-being. The current investigation involves the development, synthesis, and assessment of the antimicrobial efficacy of novel quinoline derivatives incorporating a thiosemicarbazide functionality. To design the target compounds (QST1–QST14), we applied the molecular hybridization approach to link various thiosemicarbazides to the quinoline core with a sulfonyl group. Upon the synthesis and completion of structural characterization via spectroscopic techniques (1H NMR, 13C NMR, 15N NMR, IR, and HRMS), the title molecules were extensively evaluated for their potential antitubercular, antibacterial, and antifungal activities. N-(3-Chlorophenyl)-2-(quinolin-8-ylsulfonyl)hydrazine-1-carbothioamide (QST4), the most effective compound against Mycobacterium tuberculosis H37Rv, was also tested on isoniazid-resistant clinical isolates with katG and inhA promoter mutations. Based on molecular docking studies, QST4 was also likely to demonstrate its antimycobacterial activity through inhibition of the InhA enzyme. Furthermore, three derivatives (QST3, QST4, and QST10) with preferable antimicrobial and drug-like profiles were also shown to be nontoxic against human embryonic kidney (HEK) cells. All compounds were optimized by the density functional theory method using B3LYP with the 6-31+G(d,p) basis set. Structural analysis, natural bond orbital calculations of donor–acceptor interactions, molecular electrostatic potential analysis, and frontier molecular orbital analysis were carried out. Quantum chemical descriptors and charges on the atoms were determined to compare the strengths of the intramolecular hydrogen bonds formed and their stabilities. We determined that the sulfur atom forms a stronger intramolecular hydrogen bond than the nitrogen, oxygen, and fluorine atoms in these sulfonyl thiosemicarbazide derivatives.",
journal = "ACS Omega",
title = "Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities",
pages = "40152-40140",
number = "43",
volume = "8",
doi = "10.1021/acsomega.3c03018"
}

Özcan, E., Vagolu, S. K., Gündüz, M. G., Stevanović, M., Kökbudak, Z., Tønjum, T., Nikodinović-Runić, J., Çetinkaya, Y.,& Doğan, Ş. D.. (2023). Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities. in ACS Omega, 8(43), 40140-40152.
https://doi.org/10.1021/acsomega.3c03018

Özcan E, Vagolu SK, Gündüz MG, Stevanović M, Kökbudak Z, Tønjum T, Nikodinović-Runić J, Çetinkaya Y, Doğan ŞD. Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities. in ACS Omega. 2023;8(43):40140-40152.
doi:10.1021/acsomega.3c03018 .

Özcan, Esma, Vagolu, Siva Krishna, Gündüz, Miyase Gözde, Stevanović, Milena, Kökbudak, Zülbiye, Tønjum, Tone, Nikodinović-Runić, Jasmina, Çetinkaya, Yasin, Doğan, Şengül Dilem, "Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities" in ACS Omega, 8, no. 43 (2023):40140-40152,
https://doi.org/10.1021/acsomega.3c03018 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Pantelić, Brana
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2022
AB  - Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Expression of PET-hydrolyzing enzymes in Streptomyces spp.
EP  - 836
SP  - 836
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2022
ER  - 

@conference{
author = "Stevanović, Milena and Pantelić, Brana and Janković, Vukašin and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2023",
abstract = "Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Expression of PET-hydrolyzing enzymes in Streptomyces spp.",
pages = "836-836",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2022"
}

Stevanović, M., Pantelić, B., Janković, V., Nikodinović-Runić, J.,& Vojnović, S.. (2023). Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists, 10, 836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022

Stevanović M, Pantelić B, Janković V, Nikodinović-Runić J, Vojnović S. Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists. 2023;10:836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

Stevanović, Milena, Pantelić, Brana, Janković, Vukašin, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Expression of PET-hydrolyzing enzymes in Streptomyces spp." in FEMS2023 Congress of European Microbiologists, 10 (2023):836-836,
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

TY  - JOUR
AU  - Milzarek, Tobias M.
AU  - Stevanović, Milena
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Iliasov, Denis
AU  - Wolf, Diana
AU  - Mascher, Thorsten
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2077
AB  - The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.
T2  - ACS Infectious Diseases
T2  - ACS Infectious DiseasesACS Infect. Dis.
T1  - Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs
DO  - 10.1021/acsinfecdis.3c00232
ER  - 

@article{
author = "Milzarek, Tobias M. and Stevanović, Milena and Milivojević, Dušan and Vojnović, Sandra and Iliasov, Denis and Wolf, Diana and Mascher, Thorsten and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.",
journal = "ACS Infectious Diseases, ACS Infectious DiseasesACS Infect. Dis.",
title = "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs",
doi = "10.1021/acsinfecdis.3c00232"
}

Milzarek, T. M., Stevanović, M., Milivojević, D., Vojnović, S., Iliasov, D., Wolf, D., Mascher, T., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases.
https://doi.org/10.1021/acsinfecdis.3c00232

Milzarek TM, Stevanović M, Milivojević D, Vojnović S, Iliasov D, Wolf D, Mascher T, Nikodinović-Runić J, Gulder TAM. Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases. 2023;.
doi:10.1021/acsinfecdis.3c00232 .

Milzarek, Tobias M., Stevanović, Milena, Milivojević, Dušan, Vojnović, Sandra, Iliasov, Denis, Wolf, Diana, Mascher, Thorsten, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs" in ACS Infectious Diseases (2023),
https://doi.org/10.1021/acsinfecdis.3c00232 . .

TY  - CONF
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Stevanović, Milena
AU  - Nenadović, Marija
AU  - Ilić-Tomić, Tatjana
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2026
AB  - Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Microbial live interactions with textiles
EP  - 835
EP  - 835
SP  - 835
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2026
ER  - 

@conference{
author = "Janković, Vukašin and Nikodinović-Runić, Jasmina and Radetić, Maja and Marković, Darka and Stevanović, Milena and Nenadović, Marija and Ilić-Tomić, Tatjana",
year = "2023",
abstract = "Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Microbial live interactions with textiles",
pages = "835-835-835",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2026"
}

Janković, V., Nikodinović-Runić, J., Radetić, M., Marković, D., Stevanović, M., Nenadović, M.,& Ilić-Tomić, T.. (2023). Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists, 10, 835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026

Janković V, Nikodinović-Runić J, Radetić M, Marković D, Stevanović M, Nenadović M, Ilić-Tomić T. Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists. 2023;10:835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026 .

Janković, Vukašin, Nikodinović-Runić, Jasmina, Radetić, Maja, Marković, Darka, Stevanović, Milena, Nenadović, Marija, Ilić-Tomić, Tatjana, "Microbial live interactions with textiles" in FEMS2023 Congress of European Microbiologists, 10 (2023):835-835,
https://hdl.handle.net/21.15107/rcub_imagine_2026 .

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Vukašin
AU  - Filipović, Vuk
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1915
AB  - The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365
EP  - 168
SP  - 167
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1915
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Vukašin and Filipović, Vuk and Ilić-Tomić, Tatjana and Vojnović, Sandra",
year = "2023",
abstract = "The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365",
pages = "168-167",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1915"
}

Stevanović, M., Janković, V., Filipović, V., Ilić-Tomić, T.,& Vojnović, S.. (2023). Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915

Stevanović M, Janković V, Filipović V, Ilić-Tomić T, Vojnović S. Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

Stevanović, Milena, Janković, Vukašin, Filipović, Vuk, Ilić-Tomić, Tatjana, Vojnović, Sandra, "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):167-168,
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

TY  - JOUR
AU  - Garcia, Eduardo Lanzagorta
AU  - Mojićević, Marija
AU  - Milivojević, Dušan
AU  - Aleksic, Ivana
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Murray, James
AU  - Attallah, Olivia Adly
AU  - Devine, Declan
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1658
AB  - Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates
IS  - 20
SP  - 12198
VL  - 23
DO  - 10.3390/ijms232012198
ER  - 

@article{
author = "Garcia, Eduardo Lanzagorta and Mojićević, Marija and Milivojević, Dušan and Aleksic, Ivana and Vojnović, Sandra and Stevanović, Milena and Murray, James and Attallah, Olivia Adly and Devine, Declan and Fournet, Margaret Brennan",
year = "2022",
abstract = "Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates",
number = "20",
pages = "12198",
volume = "23",
doi = "10.3390/ijms232012198"
}

Garcia, E. L., Mojićević, M., Milivojević, D., Aleksic, I., Vojnović, S., Stevanović, M., Murray, J., Attallah, O. A., Devine, D.,& Fournet, M. B.. (2022). Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences, 23(20), 12198.
https://doi.org/10.3390/ijms232012198

Garcia EL, Mojićević M, Milivojević D, Aleksic I, Vojnović S, Stevanović M, Murray J, Attallah OA, Devine D, Fournet MB. Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences. 2022;23(20):12198.
doi:10.3390/ijms232012198 .

Garcia, Eduardo Lanzagorta, Mojićević, Marija, Milivojević, Dušan, Aleksic, Ivana, Vojnović, Sandra, Stevanović, Milena, Murray, James, Attallah, Olivia Adly, Devine, Declan, Fournet, Margaret Brennan, "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates" in International Journal of Molecular Sciences, 23, no. 20 (2022):12198,
https://doi.org/10.3390/ijms232012198 . .

TY  - CONF
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Lazić, Jelena
AU  - Pantelić, Lena
AU  - Milojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1751
AB  - Iako su bioterapeutici doveli do revolucionarnih promena u lečenju raka,
dokazali svoju efikasnost u saniranju mikrobnih infekcija i lečenju ljudi sa
retkim bolestima, zbog visoke cene nisu svima dostupni. Inovativni pristup gde
otpad ulazi u sastav hranjivih podloga za gajenje bakterija bi mogao dovesti do
smanjenja cene bioterapeutika. Fermentacijom na skali od 5 L je dobijeno
nekoliko bioaktivnih prirodnih proizvoda, pri čemu je različit otpad bio izvor
hranjivih materija za gajenje mikroorganizama proizvođača. Prodigiozin,
sekundarni metabolit bakterije Serratia marcescens, dobijen je gajenjem S.
marcescens u tečnoj podlozi sa homogenizovanim mesnim nareskom, a prinos
prodigiozina je poboljšan čak 10 puta u poređenju sa standardnom podlogom.1
Kada je Pseudomonas sp. BK25H gajen u podlozi sa komponentama kuhinjskog otpada
dobijen je odličan prinos piocijanina od 12,5 mg L-1. Slično, aktinomicin D i
staurosporin, sekundarni metaboliti Streptomyces sp. BV365 i 410, su dobijeni
gajenjem proizvođača u hranjivim podlogama sa komponentama poljoprivrednog
otpada ili otpada dobijenog preradom lignoceluloze. S obzirom da neka od
navedenih jedinjenja dostižu komercijalnu vrednost od 2.500,00 evra za 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), dobijeni rezultati idu u
prilog ideji da bi se otpadne materije mogle koristiti kao jeftine sirovine za
proizvodnju vrednih hemikalija, uz istovremeno smanjenje njihove cene, pri čemu
bi se i količina otpadnih tokova redukovala.
AB  - Иако су биотерапеутици довели до револуционарних промена у лечењу рака,
доказали своју ефикасност у санирању микробних инфекција и лечењу људи са
ретким болестима, због високе цене нису свима доступни. Иновативни приступ где
отпад улази у састав храњивих подлога за гајење бактерија би могао довести до
смањења цене биотерапеутика. Ферментацијом на скали од 5 L је добијено
неколико биоактивних природних производа, при чему је различит отпад био извор
храњивих материја за гајење микроорганизама произвођача. Продигиозин,
секундарни метаболит бактерије Serratia marcescens, добијен је гајењем S.
marcescens у течној подлози са хомогенизованим месним нареском, а принос
продигиозина је побољшан чак 10 пута у поређењу са стандардном подлогом.1
Када је Pseudomonas sp. BK25H гајен у подлози са компонентама кухињског отпада
добијен је одличан принос пиоцијанина од 12,5 mg L-1. Слично, актиномицин Д и
стауроспорин, секундарни метаболити Streptomyces sp. BV365 и 410, су добијени
гајењем произвођача у храњивим подлогама са компонентама пољопривредног
отпада или отпада добијеног прерадом лигноцелулозе. С обзиром да нека од
наведених једињења достижу комерцијалну вредност од 2.500,00 евра за 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), добијени резултати иду у
прилог идеји да би се отпадне материје могле користити као јефтине сировине за
производњу вредних хемикалија, уз истовремено смањење њихове цене, при чему
би се и количина отпадних токова редуковала.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Od otpada do bioterapeutika
T1  - Од отпада до биотерапеутика
SP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1751
ER  - 

@conference{
author = "Vojnović, Sandra and Stevanović, Milena and Lazić, Jelena and Pantelić, Lena and Milojević, Dušan and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Iako su bioterapeutici doveli do revolucionarnih promena u lečenju raka,
dokazali svoju efikasnost u saniranju mikrobnih infekcija i lečenju ljudi sa
retkim bolestima, zbog visoke cene nisu svima dostupni. Inovativni pristup gde
otpad ulazi u sastav hranjivih podloga za gajenje bakterija bi mogao dovesti do
smanjenja cene bioterapeutika. Fermentacijom na skali od 5 L je dobijeno
nekoliko bioaktivnih prirodnih proizvoda, pri čemu je različit otpad bio izvor
hranjivih materija za gajenje mikroorganizama proizvođača. Prodigiozin,
sekundarni metabolit bakterije Serratia marcescens, dobijen je gajenjem S.
marcescens u tečnoj podlozi sa homogenizovanim mesnim nareskom, a prinos
prodigiozina je poboljšan čak 10 puta u poređenju sa standardnom podlogom.1
Kada je Pseudomonas sp. BK25H gajen u podlozi sa komponentama kuhinjskog otpada
dobijen je odličan prinos piocijanina od 12,5 mg L-1. Slično, aktinomicin D i
staurosporin, sekundarni metaboliti Streptomyces sp. BV365 i 410, su dobijeni
gajenjem proizvođača u hranjivim podlogama sa komponentama poljoprivrednog
otpada ili otpada dobijenog preradom lignoceluloze. S obzirom da neka od
navedenih jedinjenja dostižu komercijalnu vrednost od 2.500,00 evra za 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), dobijeni rezultati idu u
prilog ideji da bi se otpadne materije mogle koristiti kao jeftine sirovine za
proizvodnju vrednih hemikalija, uz istovremeno smanjenje njihove cene, pri čemu
bi se i količina otpadnih tokova redukovala., Иако су биотерапеутици довели до револуционарних промена у лечењу рака,
доказали своју ефикасност у санирању микробних инфекција и лечењу људи са
ретким болестима, због високе цене нису свима доступни. Иновативни приступ где
отпад улази у састав храњивих подлога за гајење бактерија би могао довести до
смањења цене биотерапеутика. Ферментацијом на скали од 5 L је добијено
неколико биоактивних природних производа, при чему је различит отпад био извор
храњивих материја за гајење микроорганизама произвођача. Продигиозин,
секундарни метаболит бактерије Serratia marcescens, добијен је гајењем S.
marcescens у течној подлози са хомогенизованим месним нареском, а принос
продигиозина је побољшан чак 10 пута у поређењу са стандардном подлогом.1
Када је Pseudomonas sp. BK25H гајен у подлози са компонентама кухињског отпада
добијен је одличан принос пиоцијанина од 12,5 mg L-1. Слично, актиномицин Д и
стауроспорин, секундарни метаболити Streptomyces sp. BV365 и 410, су добијени
гајењем произвођача у храњивим подлогама са компонентама пољопривредног
отпада или отпада добијеног прерадом лигноцелулозе. С обзиром да нека од
наведених једињења достижу комерцијалну вредност од 2.500,00 евра за 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), добијени резултати иду у
прилог идеји да би се отпадне материје могле користити као јефтине сировине за
производњу вредних хемикалија, уз истовремено смањење њихове цене, при чему
би се и количина отпадних токова редуковала.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Od otpada do bioterapeutika, Од отпада до биотерапеутика",
pages = "253",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1751"
}

Vojnović, S., Stevanović, M., Lazić, J., Pantelić, L., Milojević, D., Ilić-Tomić, T.,& Nikodinović-Runić, J.. (2022). Od otpada do bioterapeutika. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 253.
https://hdl.handle.net/21.15107/rcub_imagine_1751

Vojnović S, Stevanović M, Lazić J, Pantelić L, Milojević D, Ilić-Tomić T, Nikodinović-Runić J. Od otpada do bioterapeutika. in Treći kongres biologa Srbije. 2022;:253.
https://hdl.handle.net/21.15107/rcub_imagine_1751 .

Vojnović, Sandra, Stevanović, Milena, Lazić, Jelena, Pantelić, Lena, Milojević, Dušan, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, "Od otpada do bioterapeutika" in Treći kongres biologa Srbije (2022):253,
https://hdl.handle.net/21.15107/rcub_imagine_1751 .

TY  - CONF
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Milivojević, Dušan
AU  - Stevanović, Milena
AU  - Ilić-Tomić, Tatjana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1843
AB  - BACKGROUND
Pigments from microbial origin have promising applications in food, cosmetics, textiles and therapeutics.
Compared to other natural sources pigments from bacteria are more stable, safer, and could be cheaper to
produce and extract. Bacterial genus Streptomyces has been known as a source of biologicaly active pigments
that exhibit several effects such as antimicrobial and cytotoxic.
OBJECTIVES
The aim of this study was the isolation of pigment-producing Streptomyces strain from soil, optimization of
growth parametres for pigment production and evaluation of antimicrobial and cytotoxic activity of extracted
bacterial pigments.
METHODS
Isolation and characterization of pigment-producing Streptomyces strain from soil was done following the
standard microbiological protocol, using four different growth media. The pigments were extracted by solvent
extraction using ethyl acetate. Antimicrobial activity was analyzed using the disc diffusion test. Cytotoxic activity
was tested on the HaCaT cell line following the standard protocol.
RESULTS
Extraction of pigments by solvent extraction resulted in crude pigment extracts with antimicrobial activities.
In total pigments from 14 bacterial strains have been extracted. Antimicrobial activity was evident on Gram +
bacteria Staphylococcus aureus (6 mm of inhibition zone at concentration 8.4 μg/ml and 4 mm of inhibition
zone at concentration 1.1 μg/ml) as well as on the fungus Candida albicans (4 mm of inhibition zone at concentration
13 μg/ml). Pigments showed dose-dependent inhibiton of proliferation of HaCaT cells, with the
lowest concentration at 25 μg/ml.
C3  - FEMS Conference on Microbiology
T1  - Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts
EP  - 622
SP  - 621
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1843
ER  - 

@conference{
author = "Janković, Vukašin and Nikodinović-Runić, Jasmina and Milivojević, Dušan and Stevanović, Milena and Ilić-Tomić, Tatjana",
year = "2022",
abstract = "BACKGROUND
Pigments from microbial origin have promising applications in food, cosmetics, textiles and therapeutics.
Compared to other natural sources pigments from bacteria are more stable, safer, and could be cheaper to
produce and extract. Bacterial genus Streptomyces has been known as a source of biologicaly active pigments
that exhibit several effects such as antimicrobial and cytotoxic.
OBJECTIVES
The aim of this study was the isolation of pigment-producing Streptomyces strain from soil, optimization of
growth parametres for pigment production and evaluation of antimicrobial and cytotoxic activity of extracted
bacterial pigments.
METHODS
Isolation and characterization of pigment-producing Streptomyces strain from soil was done following the
standard microbiological protocol, using four different growth media. The pigments were extracted by solvent
extraction using ethyl acetate. Antimicrobial activity was analyzed using the disc diffusion test. Cytotoxic activity
was tested on the HaCaT cell line following the standard protocol.
RESULTS
Extraction of pigments by solvent extraction resulted in crude pigment extracts with antimicrobial activities.
In total pigments from 14 bacterial strains have been extracted. Antimicrobial activity was evident on Gram +
bacteria Staphylococcus aureus (6 mm of inhibition zone at concentration 8.4 μg/ml and 4 mm of inhibition
zone at concentration 1.1 μg/ml) as well as on the fungus Candida albicans (4 mm of inhibition zone at concentration
13 μg/ml). Pigments showed dose-dependent inhibiton of proliferation of HaCaT cells, with the
lowest concentration at 25 μg/ml.",
journal = "FEMS Conference on Microbiology",
title = "Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts",
pages = "622-621",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1843"
}

Janković, V., Nikodinović-Runić, J., Milivojević, D., Stevanović, M.,& Ilić-Tomić, T.. (2022). Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts. in FEMS Conference on Microbiology, 621-622.
https://hdl.handle.net/21.15107/rcub_imagine_1843

Janković V, Nikodinović-Runić J, Milivojević D, Stevanović M, Ilić-Tomić T. Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts. in FEMS Conference on Microbiology. 2022;:621-622.
https://hdl.handle.net/21.15107/rcub_imagine_1843 .

Janković, Vukašin, Nikodinović-Runić, Jasmina, Milivojević, Dušan, Stevanović, Milena, Ilić-Tomić, Tatjana, "Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts" in FEMS Conference on Microbiology (2022):621-622,
https://hdl.handle.net/21.15107/rcub_imagine_1843 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - D'Agostino, Paul M.
AU  - Mojicević, Marija
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1527
AB  - Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production
EP  - 2568
IS  - 4
SP  - 2560
VL  - 133
DO  - 10.1111/jam.15726
ER  - 

@article{
author = "Stevanović, Milena and D'Agostino, Paul M. and Mojicević, Marija and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2022",
abstract = "Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production",
pages = "2568-2560",
number = "4",
volume = "133",
doi = "10.1111/jam.15726"
}

Stevanović, M., D'Agostino, P. M., Mojicević, M., Gulder, T. A. M., Nikodinović-Runić, J.,& Vojnović, S.. (2022). Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology
Wiley, Hoboken., 133(4), 2560-2568.
https://doi.org/10.1111/jam.15726

Stevanović M, D'Agostino PM, Mojicević M, Gulder TAM, Nikodinović-Runić J, Vojnović S. Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology. 2022;133(4):2560-2568.
doi:10.1111/jam.15726 .

Stevanović, Milena, D'Agostino, Paul M., Mojicević, Marija, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production" in Journal of Applied Microbiology, 133, no. 4 (2022):2560-2568,
https://doi.org/10.1111/jam.15726 . .

TY  - JOUR
AU  - Gitarić, Jelena
AU  - Warzajtis, Beata
AU  - Drasković, Nenad S.
AU  - Stevanović, Milena
AU  - Ašanin, Darko P.
AU  - Škaro Bogojević, Sanja
AU  - Rychlewska, Urszula
AU  - Djuran, Milos 
AU  - Glišić, Biljana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1599
AB  - Hexadentate 2,2-dimethyl-1,3-propanediamine-N,N,N',N'-tetraacetate (2,2-diMe-1,3-pdta) ligand, containing two methyl substituents at the central carbon atom of a 1,3-propanediamine, has been prepared and used for the synthesis of Na[Cr(2,2-diMe-1,3-pdta)].3.75H2O (1) and Na[Co(2,2-diMe-1,3-pdta)].3.88H(2)O (2) complexes. These complexes were characterized by IR and electronic absorption spectroscopy, and single-crystal X-ray diffraction analysis. NMR (H-1 and C-13) spectroscopy was additionally applied for the characterization of complex 2. Crystallographic data indicate that the two investigated crystals are isostructural and contain 2,2-diMe-1,3-pdta ligand coordinated to metal ion through 2N and 4O atoms forming an octahedral complex in which the six-membered 1,3-propanediamine chelate ring adopts a twist-boat conformation. There are four such complex anions in the symmetry independent part of the unit cell. Each complex anion is further connected to the sodium counterion(s) via the bridging carboxylate group(s). Structural changes in 2,2-diMe-1,3-pdta-Cr(III) complex stimulated solely by the presence of alkyl side groups are discussed. The present study shows that in 1,3-pdtatype complexes of Cr(III) and Co(III), the environment at coordination centre can be modified by introducing substitution in one of the carbon atoms of the diamine and the resulting difference in the subunit structure can bring about noticeable change in molecular and crystal structure. The examples illustrate the importance of the steric effect for the fine tuning of the dimensionality of the resulting coordination polymer and the water content. The antimicrobial activity of complexes 1 and 2 was evaluated against different bacterial and Candida spp., while their cytotoxic effects were tested on the normal human lung fibroblast cell line (MRC-5).
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Polyhedron
T1  - Structural characterization and antimicrobial evaluation of chromium(III) and cobalt(III) complexes with 2,2-diMe-1,3-pdta: Tuning dimensionality of coordination polymer and the water content by alkyl substitution
VL  - 222
DO  - 10.1016/j.poly.2022.115864
ER  - 

@article{
author = "Gitarić, Jelena and Warzajtis, Beata and Drasković, Nenad S. and Stevanović, Milena and Ašanin, Darko P. and Škaro Bogojević, Sanja and Rychlewska, Urszula and Djuran, Milos  and Glišić, Biljana",
year = "2022",
abstract = "Hexadentate 2,2-dimethyl-1,3-propanediamine-N,N,N',N'-tetraacetate (2,2-diMe-1,3-pdta) ligand, containing two methyl substituents at the central carbon atom of a 1,3-propanediamine, has been prepared and used for the synthesis of Na[Cr(2,2-diMe-1,3-pdta)].3.75H2O (1) and Na[Co(2,2-diMe-1,3-pdta)].3.88H(2)O (2) complexes. These complexes were characterized by IR and electronic absorption spectroscopy, and single-crystal X-ray diffraction analysis. NMR (H-1 and C-13) spectroscopy was additionally applied for the characterization of complex 2. Crystallographic data indicate that the two investigated crystals are isostructural and contain 2,2-diMe-1,3-pdta ligand coordinated to metal ion through 2N and 4O atoms forming an octahedral complex in which the six-membered 1,3-propanediamine chelate ring adopts a twist-boat conformation. There are four such complex anions in the symmetry independent part of the unit cell. Each complex anion is further connected to the sodium counterion(s) via the bridging carboxylate group(s). Structural changes in 2,2-diMe-1,3-pdta-Cr(III) complex stimulated solely by the presence of alkyl side groups are discussed. The present study shows that in 1,3-pdtatype complexes of Cr(III) and Co(III), the environment at coordination centre can be modified by introducing substitution in one of the carbon atoms of the diamine and the resulting difference in the subunit structure can bring about noticeable change in molecular and crystal structure. The examples illustrate the importance of the steric effect for the fine tuning of the dimensionality of the resulting coordination polymer and the water content. The antimicrobial activity of complexes 1 and 2 was evaluated against different bacterial and Candida spp., while their cytotoxic effects were tested on the normal human lung fibroblast cell line (MRC-5).",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Polyhedron",
title = "Structural characterization and antimicrobial evaluation of chromium(III) and cobalt(III) complexes with 2,2-diMe-1,3-pdta: Tuning dimensionality of coordination polymer and the water content by alkyl substitution",
volume = "222",
doi = "10.1016/j.poly.2022.115864"
}

Gitarić, J., Warzajtis, B., Drasković, N. S., Stevanović, M., Ašanin, D. P., Škaro Bogojević, S., Rychlewska, U., Djuran, M.,& Glišić, B.. (2022). Structural characterization and antimicrobial evaluation of chromium(III) and cobalt(III) complexes with 2,2-diMe-1,3-pdta: Tuning dimensionality of coordination polymer and the water content by alkyl substitution. in Polyhedron
Pergamon-Elsevier Science Ltd, Oxford., 222.
https://doi.org/10.1016/j.poly.2022.115864

Gitarić J, Warzajtis B, Drasković NS, Stevanović M, Ašanin DP, Škaro Bogojević S, Rychlewska U, Djuran M, Glišić B. Structural characterization and antimicrobial evaluation of chromium(III) and cobalt(III) complexes with 2,2-diMe-1,3-pdta: Tuning dimensionality of coordination polymer and the water content by alkyl substitution. in Polyhedron. 2022;222.
doi:10.1016/j.poly.2022.115864 .

Gitarić, Jelena, Warzajtis, Beata, Drasković, Nenad S., Stevanović, Milena, Ašanin, Darko P., Škaro Bogojević, Sanja, Rychlewska, Urszula, Djuran, Milos , Glišić, Biljana, "Structural characterization and antimicrobial evaluation of chromium(III) and cobalt(III) complexes with 2,2-diMe-1,3-pdta: Tuning dimensionality of coordination polymer and the water content by alkyl substitution" in Polyhedron, 222 (2022),
https://doi.org/10.1016/j.poly.2022.115864 . .

TY  - JOUR
AU  - Đokić, S.
AU  - Francuz, J.
AU  - Popsavin, M.
AU  - Rodić, M.V.
AU  - Kojić, V.
AU  - Stevanović, Milena
AU  - Popsavin, V.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1546
AB  - Synthesis of protulactone A (PLA, 1) and twelve of its analogues have been achieved starting from D-galactose. PLA was isolated in the crystalline state, and its crystal structure was determined utilizing X-ray crystallography, which confirmed the assumed stereochemistry at all stereocenters. All tested compounds displayed antiproliferative activity against a panel of tumour cell lines, and all of them were non-cytotoxic toward the normal cells (MRC-5). Natural product PLA (1) was the most active against the K562 and MCF-7 cell lines (IC50 6.52 and 2.20 μM, respectively). Some of the synthesized derivatives showed very potent cytotoxicity, especially analogues 11, 13 and 15 (IC50 1.08–1.14 μM against MCF-7), and 9 and 14 (IC50 1.29 and 1.64 μM against K562). SAR analysis indicated important structural motifs for antiproliferative activity. Unfortunately, PLA (1), its C-7 epimer (2) and demethylated analogue (3) did not display a significant antimicrobial activity (two Gram-positive and two Gram-negative bacteria and one fungal strain) and they also cannot affect the ability to modulate bacterial communication.
PB  - Academic Press Inc.
T2  - Bioorganic Chemistry
T1  - Natural product protulactone A: Total synthesis from D-galactose, X-ray analysis and biological evaluation
VL  - 127
DO  - 10.1016/j.bioorg.2022.105980
ER  - 

@article{
author = "Đokić, S. and Francuz, J. and Popsavin, M. and Rodić, M.V. and Kojić, V. and Stevanović, Milena and Popsavin, V.",
year = "2022",
abstract = "Synthesis of protulactone A (PLA, 1) and twelve of its analogues have been achieved starting from D-galactose. PLA was isolated in the crystalline state, and its crystal structure was determined utilizing X-ray crystallography, which confirmed the assumed stereochemistry at all stereocenters. All tested compounds displayed antiproliferative activity against a panel of tumour cell lines, and all of them were non-cytotoxic toward the normal cells (MRC-5). Natural product PLA (1) was the most active against the K562 and MCF-7 cell lines (IC50 6.52 and 2.20 μM, respectively). Some of the synthesized derivatives showed very potent cytotoxicity, especially analogues 11, 13 and 15 (IC50 1.08–1.14 μM against MCF-7), and 9 and 14 (IC50 1.29 and 1.64 μM against K562). SAR analysis indicated important structural motifs for antiproliferative activity. Unfortunately, PLA (1), its C-7 epimer (2) and demethylated analogue (3) did not display a significant antimicrobial activity (two Gram-positive and two Gram-negative bacteria and one fungal strain) and they also cannot affect the ability to modulate bacterial communication.",
publisher = "Academic Press Inc.",
journal = "Bioorganic Chemistry",
title = "Natural product protulactone A: Total synthesis from D-galactose, X-ray analysis and biological evaluation",
volume = "127",
doi = "10.1016/j.bioorg.2022.105980"
}

Đokić, S., Francuz, J., Popsavin, M., Rodić, M.V., Kojić, V., Stevanović, M.,& Popsavin, V.. (2022). Natural product protulactone A: Total synthesis from D-galactose, X-ray analysis and biological evaluation. in Bioorganic Chemistry
Academic Press Inc.., 127.
https://doi.org/10.1016/j.bioorg.2022.105980

Đokić S, Francuz J, Popsavin M, Rodić M, Kojić V, Stevanović M, Popsavin V. Natural product protulactone A: Total synthesis from D-galactose, X-ray analysis and biological evaluation. in Bioorganic Chemistry. 2022;127.
doi:10.1016/j.bioorg.2022.105980 .

Đokić, S., Francuz, J., Popsavin, M., Rodić, M.V., Kojić, V., Stevanović, Milena, Popsavin, V., "Natural product protulactone A: Total synthesis from D-galactose, X-ray analysis and biological evaluation" in Bioorganic Chemistry, 127 (2022),
https://doi.org/10.1016/j.bioorg.2022.105980 . .

TY  - JOUR
AU  - Gitarić, Jelena
AU  - Stanojević, Ivana M.
AU  - Rodić, Marko, V
AU  - Drasković, Nenad S.
AU  - Stevanović, Milena
AU  - Vojnović, Sandra
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1352
AB  - New polynuclear manganese(II) and cadmium(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N, N',N'-tetraacetato ligand (2,2-diMe-1,3-pdta), {Ba[M(2,2-diMe-1,3-pdta)]center dot 3H(2)O}(n) (M = Mn (1) or Cd (2)) were synthesized and characterized by IR spectroscopy and single-crystal X-ray diffraction analysis. In addition, complex 2 was characterized by solution H-1 and C-13 NMR spectroscopy. Crystallographic analysis showed that 2,2-diMe-1,3-pdta ligand is hexadentately coordinated to each M(II) ion through the two nitrogen and four carboxylate oxygen atoms, whereas the one of these oxygen atoms is also involved in coordination to the second M(II) ion of the dinuclear [M-2(2,2-diMe-1,3-pdta)(2)](4-) unit in polymeric structures. Moreover, three of four carboxylic groups of 2,2-diMe-1,3-pdta ligand are additionally bonded to four Ba(II) ions, in three distinctive bridging coordination modes. Each Ba(II) ion is surrounded by ten oxygen atoms, seven belonging to carboxylate groups of 2,2-diMe-1,3-pdta, and three belonging to water molecules. The coordination environment around Mn(II) and Cd(II) ions could be assigned as a face capped octahedron, while coordination polyhedron around Ba(II) ion in these two complexes was described as a distorted sphenocorona. The antimicrobial potential of complexes 1 and 2 and corresponding metal salts used for their synthesis was evaluated against different bacterial and Candida spp. Both complexes showed selective antifungal activity against the tested Candida spp. compared to the bacterial strains, with the minimal inhibitory concentration (MIC) values in the range 3.12 - 12.50 mu M. Moreover, complex 1 caused the slightly decrease of hyphae length, while no significant influence on hyphal length of complex 2 was observed. With aim to assess the therapeutic profile of the complexes, their cytotoxicity was evaluated against the normal human lung fibroblast cell line (MRC-5).
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Polyhedron
T1  - Structural characterization and biological evaluation of polynuclear Mn (II) and Cd(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N,N ', N '-tetraacetate. The influence of ligand structure and counter cation on the complex nuclearity
VL  - 188
DO  - 10.1016/j.poly.2020.114688
ER  - 

@article{
author = "Gitarić, Jelena and Stanojević, Ivana M. and Rodić, Marko, V and Drasković, Nenad S. and Stevanović, Milena and Vojnović, Sandra and Djuran, Milos and Glišić, Biljana",
year = "2020",
abstract = "New polynuclear manganese(II) and cadmium(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N, N',N'-tetraacetato ligand (2,2-diMe-1,3-pdta), {Ba[M(2,2-diMe-1,3-pdta)]center dot 3H(2)O}(n) (M = Mn (1) or Cd (2)) were synthesized and characterized by IR spectroscopy and single-crystal X-ray diffraction analysis. In addition, complex 2 was characterized by solution H-1 and C-13 NMR spectroscopy. Crystallographic analysis showed that 2,2-diMe-1,3-pdta ligand is hexadentately coordinated to each M(II) ion through the two nitrogen and four carboxylate oxygen atoms, whereas the one of these oxygen atoms is also involved in coordination to the second M(II) ion of the dinuclear [M-2(2,2-diMe-1,3-pdta)(2)](4-) unit in polymeric structures. Moreover, three of four carboxylic groups of 2,2-diMe-1,3-pdta ligand are additionally bonded to four Ba(II) ions, in three distinctive bridging coordination modes. Each Ba(II) ion is surrounded by ten oxygen atoms, seven belonging to carboxylate groups of 2,2-diMe-1,3-pdta, and three belonging to water molecules. The coordination environment around Mn(II) and Cd(II) ions could be assigned as a face capped octahedron, while coordination polyhedron around Ba(II) ion in these two complexes was described as a distorted sphenocorona. The antimicrobial potential of complexes 1 and 2 and corresponding metal salts used for their synthesis was evaluated against different bacterial and Candida spp. Both complexes showed selective antifungal activity against the tested Candida spp. compared to the bacterial strains, with the minimal inhibitory concentration (MIC) values in the range 3.12 - 12.50 mu M. Moreover, complex 1 caused the slightly decrease of hyphae length, while no significant influence on hyphal length of complex 2 was observed. With aim to assess the therapeutic profile of the complexes, their cytotoxicity was evaluated against the normal human lung fibroblast cell line (MRC-5).",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Polyhedron",
title = "Structural characterization and biological evaluation of polynuclear Mn (II) and Cd(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N,N ', N '-tetraacetate. The influence of ligand structure and counter cation on the complex nuclearity",
volume = "188",
doi = "10.1016/j.poly.2020.114688"
}

Gitarić, J., Stanojević, I. M., Rodić, M. V., Drasković, N. S., Stevanović, M., Vojnović, S., Djuran, M.,& Glišić, B.. (2020). Structural characterization and biological evaluation of polynuclear Mn (II) and Cd(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N,N ', N '-tetraacetate. The influence of ligand structure and counter cation on the complex nuclearity. in Polyhedron
Pergamon-Elsevier Science Ltd, Oxford., 188.
https://doi.org/10.1016/j.poly.2020.114688

Gitarić J, Stanojević IM, Rodić MV, Drasković NS, Stevanović M, Vojnović S, Djuran M, Glišić B. Structural characterization and biological evaluation of polynuclear Mn (II) and Cd(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N,N ', N '-tetraacetate. The influence of ligand structure and counter cation on the complex nuclearity. in Polyhedron. 2020;188.
doi:10.1016/j.poly.2020.114688 .

Gitarić, Jelena, Stanojević, Ivana M., Rodić, Marko, V, Drasković, Nenad S., Stevanović, Milena, Vojnović, Sandra, Djuran, Milos, Glišić, Biljana, "Structural characterization and biological evaluation of polynuclear Mn (II) and Cd(II) complexes with 2,2-dimethyl-1,3-propanediamine-N,N,N ', N '-tetraacetate. The influence of ligand structure and counter cation on the complex nuclearity" in Polyhedron, 188 (2020),
https://doi.org/10.1016/j.poly.2020.114688 . .