TY  - JOUR
AU  - Pantelić, Brana
AU  - Araujo, Jeovan
AU  - Jeremić, Sanja
AU  - Azeem, Muhammad
AU  - Attallah, Olivia
AU  - Slaperas, Romanos
AU  - Mojicević, Marija
AU  - Chen, Yuanyuan
AU  - Fournet, Margaret Brennan
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2352186423003127
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1983
AB  - Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.
PB  - Elsevier
T2  - Environmental Technology & Innovation
T1  - A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate
SP  - 103316
DO  - 10.1016/j.eti.2023.103316
ER  - 

@article{
author = "Pantelić, Brana and Araujo, Jeovan and Jeremić, Sanja and Azeem, Muhammad and Attallah, Olivia and Slaperas, Romanos and Mojicević, Marija and Chen, Yuanyuan and Fournet, Margaret Brennan and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.",
publisher = "Elsevier",
journal = "Environmental Technology & Innovation",
title = "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate",
pages = "103316",
doi = "10.1016/j.eti.2023.103316"
}

Pantelić, B., Araujo, J., Jeremić, S., Azeem, M., Attallah, O., Slaperas, R., Mojicević, M., Chen, Y., Fournet, M. B., Topakas, E.,& Nikodinović-Runić, J.. (2023). A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation
Elsevier., 103316.
https://doi.org/10.1016/j.eti.2023.103316

Pantelić B, Araujo J, Jeremić S, Azeem M, Attallah O, Slaperas R, Mojicević M, Chen Y, Fournet MB, Topakas E, Nikodinović-Runić J. A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation. 2023;:103316.
doi:10.1016/j.eti.2023.103316 .

Pantelić, Brana, Araujo, Jeovan, Jeremić, Sanja, Azeem, Muhammad, Attallah, Olivia, Slaperas, Romanos, Mojicević, Marija, Chen, Yuanyuan, Fournet, Margaret Brennan, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate" in Environmental Technology & Innovation (2023):103316,
https://doi.org/10.1016/j.eti.2023.103316 . .

TY  - CONF
AU  - Garza Herrera, Diana Alicia
AU  - Mojicević, Marija
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2234
AB  - Background: Renowned oil-based polymers represent
an environmental nuisance, due to their well-known
permanency in nature, known to disrupt ecosystems.
In response, bio-based polymers have emerged as an
alternative, providing a sustainable blueprint for
plastic circularity1. However, despite being classified
as biodegradable, these polymers can still show
resistance to biodegradation when not presented
with optimal environmental conditions such as
specific temperature and pH, amongst other key
factors in their breakdown. Due to this, microbial
degradation results in an interesting path to promote
biodegradation of such polymers.2
Objective: This study aims to evaluate the ability of
selected strains isolated from polluted environments
to improve the rate of biopolymers’ biodegradation
such as Polyhydroxy butyrate (PHB), Polylactic acid
(PLA), and Bacterial cellulose (BC) when exposed to
strains with specific enzymatic tools to aid in its
degradation.
Methods: Selected strains were previously isolated
from plastic-polluted soil samples. Strains were
transferred to Mineral Salt media (MSM) biopolymersupplemented
plates (7d, 30℃) (MSM (15 g/l Agar, 9
g/l Na2HPO4 x 12H2O, 1.5 g/l KH2PO4, 1 g/l NH4Cl,
0.2 g/l MgSO4 x 7H2O, 0.2 g/l CaCl2 x 2H2O,
Fe(III)NH4-citrate 0.0012 g/l), (1-3%) biopolymers
(PHB, BC, and PLA) growth and clear-zone method
were used to determine strains’ possible enzymatic
activity. Selected strains were transferred to flasks
containing PHB, PLA, and BC films, respectively, in
MSM and incubated for 20 days at 30℃, 120 rpm.
Samples were washed and dried and their level of
degradation was assessed by FTIR, weight loss, and
scanning electron microscopy (SEM). The ability of the
best performer strain to degrade PHB was
additionally assessed using Respirometer (Echo
Instruments) to determine the biodegradability of
said films under a controlled temperature (25°C) with
a flow rate of 500ml/min.
Results: Significant weight loss was observed in
samples exposed to strains, meaning biodegradability
was achieved in an important percentage, proving
their capability to degrade the proposed
biopolymers, compared to samples lacking microbial
presence.
C3  - 10th Conference of Mikrobiokosmos,
T1  - Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2234
ER  - 

@conference{
author = "Garza Herrera, Diana Alicia and Mojicević, Marija and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Background: Renowned oil-based polymers represent
an environmental nuisance, due to their well-known
permanency in nature, known to disrupt ecosystems.
In response, bio-based polymers have emerged as an
alternative, providing a sustainable blueprint for
plastic circularity1. However, despite being classified
as biodegradable, these polymers can still show
resistance to biodegradation when not presented
with optimal environmental conditions such as
specific temperature and pH, amongst other key
factors in their breakdown. Due to this, microbial
degradation results in an interesting path to promote
biodegradation of such polymers.2
Objective: This study aims to evaluate the ability of
selected strains isolated from polluted environments
to improve the rate of biopolymers’ biodegradation
such as Polyhydroxy butyrate (PHB), Polylactic acid
(PLA), and Bacterial cellulose (BC) when exposed to
strains with specific enzymatic tools to aid in its
degradation.
Methods: Selected strains were previously isolated
from plastic-polluted soil samples. Strains were
transferred to Mineral Salt media (MSM) biopolymersupplemented
plates (7d, 30℃) (MSM (15 g/l Agar, 9
g/l Na2HPO4 x 12H2O, 1.5 g/l KH2PO4, 1 g/l NH4Cl,
0.2 g/l MgSO4 x 7H2O, 0.2 g/l CaCl2 x 2H2O,
Fe(III)NH4-citrate 0.0012 g/l), (1-3%) biopolymers
(PHB, BC, and PLA) growth and clear-zone method
were used to determine strains’ possible enzymatic
activity. Selected strains were transferred to flasks
containing PHB, PLA, and BC films, respectively, in
MSM and incubated for 20 days at 30℃, 120 rpm.
Samples were washed and dried and their level of
degradation was assessed by FTIR, weight loss, and
scanning electron microscopy (SEM). The ability of the
best performer strain to degrade PHB was
additionally assessed using Respirometer (Echo
Instruments) to determine the biodegradability of
said films under a controlled temperature (25°C) with
a flow rate of 500ml/min.
Results: Significant weight loss was observed in
samples exposed to strains, meaning biodegradability
was achieved in an important percentage, proving
their capability to degrade the proposed
biopolymers, compared to samples lacking microbial
presence.",
journal = "10th Conference of Mikrobiokosmos,",
title = "Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2234"
}

Garza Herrera, D. A., Mojicević, M.,& Nikodinović-Runić, J.. (2023). Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation. in 10th Conference of Mikrobiokosmos,.
https://hdl.handle.net/21.15107/rcub_imagine_2234

Garza Herrera DA, Mojicević M, Nikodinović-Runić J. Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation. in 10th Conference of Mikrobiokosmos,. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2234 .

Garza Herrera, Diana Alicia, Mojicević, Marija, Nikodinović-Runić, Jasmina, "Exploring the enzymatic ability of strains isolated from plastic-polluted environments for enhancing synthetic and natural biopolymers’ biodegradation" in 10th Conference of Mikrobiokosmos, (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2234 .

TY  - JOUR
AU  - Herrera, Diana A. Garza
AU  - Mojićević, Marija
AU  - Pantelić, Brana
AU  - Joshi, Akanksha
AU  - Collins, Catherine
AU  - Batista, Maria
AU  - Torres, Cristiana
AU  - Freitas, Filomena
AU  - Murray, Patrick
AU  - Nikodinović-Runić, Jasmina
AU  - Brennan Fournet, Margaret
PY  - 2023
UR  - https://www.mdpi.com/2076-2607/11/12/2914
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2225
AB  - The exposure of microorganisms to conventional plastics is a relatively recent occurrence, affording limited time for evolutionary adaptation. As part of the EU-funded project BioICEP, this study delves into the plastic degradation potential of microorganisms isolated from sites with prolonged plastic pollution, such as plastic-polluted forests, biopolymer-contaminated soil, oil-contaminated soil, municipal landfill, but also a distinctive soil sample with plastic pieces buried three decades ago. Additionally, samples from Arthropoda species were investigated. In total, 150 strains were isolated and screened for the ability to use plastic-related substrates (Impranil dispersions, polyethylene terephthalate, terephthalic acid, and bis(2-hydroxyethyl) terephthalate). Twenty isolates selected based on their ability to grow on various substrates were identified as Streptomyces, Bacillus, Enterococcus, and Pseudomonas spp. Morphological features were recorded, and the 16S rRNA sequence was employed to construct a phylogenetic tree. Subsequent assessments unveiled that 5 out of the 20 strains displayed the capability to produce polyhydroxyalkanoates, utilizing pre-treated post-consumer PET samples. With Priestia sp. DG69 and Neobacillus sp. DG40 emerging as the most successful producers (4.14% and 3.34% of PHA, respectively), these strains are poised for further utilization in upcycling purposes, laying the foundation for the development of sustainable strategies for plastic waste management.
T2  - Microorganisms
T2  - Microorganisms
T1  - Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential
IS  - 12
SP  - 2914
VL  - 11
DO  - 10.3390/microorganisms11122914
ER  - 

@article{
author = "Herrera, Diana A. Garza and Mojićević, Marija and Pantelić, Brana and Joshi, Akanksha and Collins, Catherine and Batista, Maria and Torres, Cristiana and Freitas, Filomena and Murray, Patrick and Nikodinović-Runić, Jasmina and Brennan Fournet, Margaret",
year = "2023",
abstract = "The exposure of microorganisms to conventional plastics is a relatively recent occurrence, affording limited time for evolutionary adaptation. As part of the EU-funded project BioICEP, this study delves into the plastic degradation potential of microorganisms isolated from sites with prolonged plastic pollution, such as plastic-polluted forests, biopolymer-contaminated soil, oil-contaminated soil, municipal landfill, but also a distinctive soil sample with plastic pieces buried three decades ago. Additionally, samples from Arthropoda species were investigated. In total, 150 strains were isolated and screened for the ability to use plastic-related substrates (Impranil dispersions, polyethylene terephthalate, terephthalic acid, and bis(2-hydroxyethyl) terephthalate). Twenty isolates selected based on their ability to grow on various substrates were identified as Streptomyces, Bacillus, Enterococcus, and Pseudomonas spp. Morphological features were recorded, and the 16S rRNA sequence was employed to construct a phylogenetic tree. Subsequent assessments unveiled that 5 out of the 20 strains displayed the capability to produce polyhydroxyalkanoates, utilizing pre-treated post-consumer PET samples. With Priestia sp. DG69 and Neobacillus sp. DG40 emerging as the most successful producers (4.14% and 3.34% of PHA, respectively), these strains are poised for further utilization in upcycling purposes, laying the foundation for the development of sustainable strategies for plastic waste management.",
journal = "Microorganisms, Microorganisms",
title = "Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential",
number = "12",
pages = "2914",
volume = "11",
doi = "10.3390/microorganisms11122914"
}

Herrera, D. A. G., Mojićević, M., Pantelić, B., Joshi, A., Collins, C., Batista, M., Torres, C., Freitas, F., Murray, P., Nikodinović-Runić, J.,& Brennan Fournet, M.. (2023). Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential. in Microorganisms, 11(12), 2914.
https://doi.org/10.3390/microorganisms11122914

Herrera DAG, Mojićević M, Pantelić B, Joshi A, Collins C, Batista M, Torres C, Freitas F, Murray P, Nikodinović-Runić J, Brennan Fournet M. Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential. in Microorganisms. 2023;11(12):2914.
doi:10.3390/microorganisms11122914 .

Herrera, Diana A. Garza, Mojićević, Marija, Pantelić, Brana, Joshi, Akanksha, Collins, Catherine, Batista, Maria, Torres, Cristiana, Freitas, Filomena, Murray, Patrick, Nikodinović-Runić, Jasmina, Brennan Fournet, Margaret, "Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential" in Microorganisms, 11, no. 12 (2023):2914,
https://doi.org/10.3390/microorganisms11122914 . .

TY  - JOUR
AU  - Garcia, Eduardo Lanzagorta
AU  - Mojićević, Marija
AU  - Milivojević, Dušan
AU  - Aleksic, Ivana
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Murray, James
AU  - Attallah, Olivia Adly
AU  - Devine, Declan
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1658
AB  - Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates
IS  - 20
SP  - 12198
VL  - 23
DO  - 10.3390/ijms232012198
ER  - 

@article{
author = "Garcia, Eduardo Lanzagorta and Mojićević, Marija and Milivojević, Dušan and Aleksic, Ivana and Vojnović, Sandra and Stevanović, Milena and Murray, James and Attallah, Olivia Adly and Devine, Declan and Fournet, Margaret Brennan",
year = "2022",
abstract = "Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates",
number = "20",
pages = "12198",
volume = "23",
doi = "10.3390/ijms232012198"
}

Garcia, E. L., Mojićević, M., Milivojević, D., Aleksic, I., Vojnović, S., Stevanović, M., Murray, J., Attallah, O. A., Devine, D.,& Fournet, M. B.. (2022). Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences, 23(20), 12198.
https://doi.org/10.3390/ijms232012198

Garcia EL, Mojićević M, Milivojević D, Aleksic I, Vojnović S, Stevanović M, Murray J, Attallah OA, Devine D, Fournet MB. Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences. 2022;23(20):12198.
doi:10.3390/ijms232012198 .

Garcia, Eduardo Lanzagorta, Mojićević, Marija, Milivojević, Dušan, Aleksic, Ivana, Vojnović, Sandra, Stevanović, Milena, Murray, James, Attallah, Olivia Adly, Devine, Declan, Fournet, Margaret Brennan, "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates" in International Journal of Molecular Sciences, 23, no. 20 (2022):12198,
https://doi.org/10.3390/ijms232012198 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - D'Agostino, Paul M.
AU  - Mojicević, Marija
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1527
AB  - Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production
EP  - 2568
IS  - 4
SP  - 2560
VL  - 133
DO  - 10.1111/jam.15726
ER  - 

@article{
author = "Stevanović, Milena and D'Agostino, Paul M. and Mojicević, Marija and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2022",
abstract = "Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production",
pages = "2568-2560",
number = "4",
volume = "133",
doi = "10.1111/jam.15726"
}

Stevanović, M., D'Agostino, P. M., Mojicević, M., Gulder, T. A. M., Nikodinović-Runić, J.,& Vojnović, S.. (2022). Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology
Wiley, Hoboken., 133(4), 2560-2568.
https://doi.org/10.1111/jam.15726

Stevanović M, D'Agostino PM, Mojicević M, Gulder TAM, Nikodinović-Runić J, Vojnović S. Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology. 2022;133(4):2560-2568.
doi:10.1111/jam.15726 .

Stevanović, Milena, D'Agostino, Paul M., Mojicević, Marija, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production" in Journal of Applied Microbiology, 133, no. 4 (2022):2560-2568,
https://doi.org/10.1111/jam.15726 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Milovanović, Jelena
AU  - Mojicević, Marija
AU  - Škaro Bogojević, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1343
AB  - Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Understanding bioplastic materials - Current state and trends
EP  - 1538
IS  - 12
SP  - 1507
VL  - 85
DO  - 10.2298/JSC200720051J
ER  - 

@article{
author = "Jeremić, Sanja and Milovanović, Jelena and Mojicević, Marija and Škaro Bogojević, Sanja and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Understanding bioplastic materials - Current state and trends",
pages = "1538-1507",
number = "12",
volume = "85",
doi = "10.2298/JSC200720051J"
}

Jeremić, S., Milovanović, J., Mojicević, M., Škaro Bogojević, S.,& Nikodinović-Runić, J.. (2020). Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(12), 1507-1538.
https://doi.org/10.2298/JSC200720051J

Jeremić S, Milovanović J, Mojicević M, Škaro Bogojević S, Nikodinović-Runić J. Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society. 2020;85(12):1507-1538.
doi:10.2298/JSC200720051J .

Jeremić, Sanja, Milovanović, Jelena, Mojicević, Marija, Škaro Bogojević, Sanja, Nikodinović-Runić, Jasmina, "Understanding bioplastic materials - Current state and trends" in Journal of the Serbian Chemical Society, 85, no. 12 (2020):1507-1538,
https://doi.org/10.2298/JSC200720051J . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Senthamaraikannan, Ramsankar
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1384
AB  - Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.
PB  - Wiley, Hoboken
T2  - Microbiologyopen
T1  - Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
IS  - 3
VL  - 9
DO  - 10.1002/mbo3.986
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Pavić, Aleksandar and Vojnović, Sandra and Senthamaraikannan, Ramsankar and Vasiljević, Branka and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.",
publisher = "Wiley, Hoboken",
journal = "Microbiologyopen",
title = "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties",
number = "3",
volume = "9",
doi = "10.1002/mbo3.986"
}

Mojicević, M., D'Agostino, P. M., Pavić, A., Vojnović, S., Senthamaraikannan, R., Vasiljević, B., Gulder, T. A. M.,& Nikodinović-Runić, J.. (2020). Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen
Wiley, Hoboken., 9(3).
https://doi.org/10.1002/mbo3.986

Mojicević M, D'Agostino PM, Pavić A, Vojnović S, Senthamaraikannan R, Vasiljević B, Gulder TAM, Nikodinović-Runić J. Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen. 2020;9(3).
doi:10.1002/mbo3.986 .

Mojicević, Marija, D'Agostino, Paul M., Pavić, Aleksandar, Vojnović, Sandra, Senthamaraikannan, Ramsankar, Vasiljević, Branka, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties" in Microbiologyopen, 9, no. 3 (2020),
https://doi.org/10.1002/mbo3.986 . .

TY  - JOUR
AU  - Durić, Sonja
AU  - Vojnović, Sandra
AU  - Pavić, Aleksandar
AU  - Mojicević, Marija
AU  - Wadepohl, Hubert
AU  - Savić, Nada D.
AU  - Popsavin, Mirjana
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos 
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1377
AB  - New polynuclear silver(I) complexes with 1,5-naphthyridine (1,5-naph), [Ag(NO3)(1,5-naph)](n) (Ag1), [Ag (CF3COO)(1,5-naph)]n (Ag2) and [Ag(CF3SO3)(1,5-naph)](n) (Ag3) were synthesized by the reaction of the corresponding silver(I) salt and 1,5-naph in ethanol at room temperature. These complexes were characterized by NMR, IR and UV Vis spectroscopy, while their crystal structures were determined by single-crystal X-ray diffraction analysis. In all these complexes, 1,5-naph acts as a bridging ligand between two Ag(I) ions, while the remaining coordination sites are occupied by oxygen atom(s) of the corresponding anion. The antimicrobial efficiency of these silver(I) complexes was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The complexes showed good to moderate antibacterial activity with the minimal inhibitory concentration (MIC) values being in the range 2.5-100 mu g/mL (6.5-333.3 mu M), while their antifungal activity against the investigated Candida spp. was significantly higher (MIC = 0.78-6.25 mu g/mL; 2.6-20.8 mu M). Moreover, complexes Ag1 and Ag2 effectively inhibited C. albicans biofilms formation, while Ag1 was also shown to inhibit the formation of mixed C. albicans/Pseudomonas aeruginosa biofilms. Toxicological evaluations on zebrafish (Dario rerio) embryos revealed that all silver(I) complexes could be applied as antifungal agents, whereas Ag3 had the best therapeutic potential showing both the lowest MIC values against the tested Candida strains and the non-toxic in vivo response in the zebrafish embryos at these doses.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center
VL  - 203
DO  - 10.1016/j.jinorgbio.2019.110872
ER  - 

@article{
author = "Durić, Sonja and Vojnović, Sandra and Pavić, Aleksandar and Mojicević, Marija and Wadepohl, Hubert and Savić, Nada D. and Popsavin, Mirjana and Nikodinović-Runić, Jasmina and Djuran, Milos  and Glišić, Biljana",
year = "2020",
abstract = "New polynuclear silver(I) complexes with 1,5-naphthyridine (1,5-naph), [Ag(NO3)(1,5-naph)](n) (Ag1), [Ag (CF3COO)(1,5-naph)]n (Ag2) and [Ag(CF3SO3)(1,5-naph)](n) (Ag3) were synthesized by the reaction of the corresponding silver(I) salt and 1,5-naph in ethanol at room temperature. These complexes were characterized by NMR, IR and UV Vis spectroscopy, while their crystal structures were determined by single-crystal X-ray diffraction analysis. In all these complexes, 1,5-naph acts as a bridging ligand between two Ag(I) ions, while the remaining coordination sites are occupied by oxygen atom(s) of the corresponding anion. The antimicrobial efficiency of these silver(I) complexes was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The complexes showed good to moderate antibacterial activity with the minimal inhibitory concentration (MIC) values being in the range 2.5-100 mu g/mL (6.5-333.3 mu M), while their antifungal activity against the investigated Candida spp. was significantly higher (MIC = 0.78-6.25 mu g/mL; 2.6-20.8 mu M). Moreover, complexes Ag1 and Ag2 effectively inhibited C. albicans biofilms formation, while Ag1 was also shown to inhibit the formation of mixed C. albicans/Pseudomonas aeruginosa biofilms. Toxicological evaluations on zebrafish (Dario rerio) embryos revealed that all silver(I) complexes could be applied as antifungal agents, whereas Ag3 had the best therapeutic potential showing both the lowest MIC values against the tested Candida strains and the non-toxic in vivo response in the zebrafish embryos at these doses.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center",
volume = "203",
doi = "10.1016/j.jinorgbio.2019.110872"
}

Durić, S., Vojnović, S., Pavić, A., Mojicević, M., Wadepohl, H., Savić, N. D., Popsavin, M., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 203.
https://doi.org/10.1016/j.jinorgbio.2019.110872

Durić S, Vojnović S, Pavić A, Mojicević M, Wadepohl H, Savić ND, Popsavin M, Nikodinović-Runić J, Djuran M, Glišić B. New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center. in Journal of Inorganic Biochemistry. 2020;203.
doi:10.1016/j.jinorgbio.2019.110872 .

Durić, Sonja, Vojnović, Sandra, Pavić, Aleksandar, Mojicević, Marija, Wadepohl, Hubert, Savić, Nada D., Popsavin, Mirjana, Nikodinović-Runić, Jasmina, Djuran, Milos , Glišić, Biljana, "New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center" in Journal of Inorganic Biochemistry, 203 (2020),
https://doi.org/10.1016/j.jinorgbio.2019.110872 . .

TY  - JOUR
AU  - Savić, Nada D.
AU  - Petković, Branka B.
AU  - Vojnović, Sandra
AU  - Mojicević, Marija
AU  - Wadepohl, Hubert
AU  - Olaifa, Kayode
AU  - Marsili, Enrico
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1313
AB  - New dinuclear silver(i) complexes withN,N ',N '',N '''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), [Ag-2(NO3)(tpmc)]NO3 center dot 1.7H(2)O (1), [Ag-2(CF3SO3)(2)(tpmc)] (2), and [Ag-2(tpmc)](BF4)(2) (3) were synthesized and characterized by NMR (H-1 and(13)C), IR and UV- Vis spectroscopy, cyclic voltammetry and molar conductivity measurements. The molecular structures of the complexes were determined by single-crystal X-ray diffraction analysis. The spectroscopic and crystallographic data showed that the structure of the complexes strongly depends on the nature of the counteranion of silver(i) salt used for their synthesis. The antimicrobial activity of complexes1-3was examined against Gram-positive and Gram-negative bacteria and different species of unicellular fungus Candida spp. The ability of these complexes to inhibit the formation of Candida biofilms and to eradicate the already formed biofilms was tested in the standard microtiter plate-based assay. In addition, a bioelectrochemical testing of the antimicrobial activity of complex 1 against early biofilm was also performed. The obtained results indicated that complexes 1-3 showed increased activity toward Gram-negative bacteria and Candida spp. and could inhibit the formation of biofilms. In most cases, these complexes had positive selectivity indices and showed similar or even better activity with respect to the clinically used silver(i) sulfadiazine (AgSD). The values of the binding constants for complexes 1-3 to bovine serum albumin (BSA) were found to be high enough to indicate their binding to this biomolecule, but not so high as to prevent their release upon arrival at the target site. Moreover, the positive values of partition coefficients for these complexes indicated their ability to be transported through the cell membrane. Once inside the cell, complexes 1-3 could induce the formation of the reactive oxygen species (ROS) in C. albicanscells and/or interact with DNA. Taken together, silver(i) complexes with the tpmc ligand could be considered as novel antimicrobial compounds with favourable pharmacological properties, being safer than AgSD.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes
EP  - 10894
IS  - 31
SP  - 10880
VL  - 49
DO  - 10.1039/d0dt01272f
ER  - 

@article{
author = "Savić, Nada D. and Petković, Branka B. and Vojnović, Sandra and Mojicević, Marija and Wadepohl, Hubert and Olaifa, Kayode and Marsili, Enrico and Nikodinović-Runić, Jasmina and Djuran, Milos and Glišić, Biljana",
year = "2020",
abstract = "New dinuclear silver(i) complexes withN,N ',N '',N '''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc), [Ag-2(NO3)(tpmc)]NO3 center dot 1.7H(2)O (1), [Ag-2(CF3SO3)(2)(tpmc)] (2), and [Ag-2(tpmc)](BF4)(2) (3) were synthesized and characterized by NMR (H-1 and(13)C), IR and UV- Vis spectroscopy, cyclic voltammetry and molar conductivity measurements. The molecular structures of the complexes were determined by single-crystal X-ray diffraction analysis. The spectroscopic and crystallographic data showed that the structure of the complexes strongly depends on the nature of the counteranion of silver(i) salt used for their synthesis. The antimicrobial activity of complexes1-3was examined against Gram-positive and Gram-negative bacteria and different species of unicellular fungus Candida spp. The ability of these complexes to inhibit the formation of Candida biofilms and to eradicate the already formed biofilms was tested in the standard microtiter plate-based assay. In addition, a bioelectrochemical testing of the antimicrobial activity of complex 1 against early biofilm was also performed. The obtained results indicated that complexes 1-3 showed increased activity toward Gram-negative bacteria and Candida spp. and could inhibit the formation of biofilms. In most cases, these complexes had positive selectivity indices and showed similar or even better activity with respect to the clinically used silver(i) sulfadiazine (AgSD). The values of the binding constants for complexes 1-3 to bovine serum albumin (BSA) were found to be high enough to indicate their binding to this biomolecule, but not so high as to prevent their release upon arrival at the target site. Moreover, the positive values of partition coefficients for these complexes indicated their ability to be transported through the cell membrane. Once inside the cell, complexes 1-3 could induce the formation of the reactive oxygen species (ROS) in C. albicanscells and/or interact with DNA. Taken together, silver(i) complexes with the tpmc ligand could be considered as novel antimicrobial compounds with favourable pharmacological properties, being safer than AgSD.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes",
pages = "10894-10880",
number = "31",
volume = "49",
doi = "10.1039/d0dt01272f"
}

Savić, N. D., Petković, B. B., Vojnović, S., Mojicević, M., Wadepohl, H., Olaifa, K., Marsili, E., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 49(31), 10880-10894.
https://doi.org/10.1039/d0dt01272f

Savić ND, Petković BB, Vojnović S, Mojicević M, Wadepohl H, Olaifa K, Marsili E, Nikodinović-Runić J, Djuran M, Glišić B. Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes. in Dalton Transactions. 2020;49(31):10880-10894.
doi:10.1039/d0dt01272f .

Savić, Nada D., Petković, Branka B., Vojnović, Sandra, Mojicević, Marija, Wadepohl, Hubert, Olaifa, Kayode, Marsili, Enrico, Nikodinović-Runić, Jasmina, Djuran, Milos, Glišić, Biljana, "Dinuclear silver(I) complexes with a pyridine-based macrocyclic type of ligand as antimicrobial agents against clinically relevant species: the influence of the counteranion on the structure diversification of the complexes" in Dalton Transactions, 49, no. 31 (2020):10880-10894,
https://doi.org/10.1039/d0dt01272f . .

TY  - JOUR
AU  - Andrejević, Tina P.
AU  - Warzajtis, Beata
AU  - Glišić, Biljana
AU  - Vojnović, Sandra
AU  - Mojicević, Marija
AU  - Stevanović, Nevena Lj.
AU  - Nikodinović-Runić, Jasmina
AU  - Rychlewska, Urszula
AU  - Djuran, Milos
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1325
AB  - Three novel Zn(II) complexes, [ZnCl2(qz)(2)] (1), [ZnCl2(1,5-naph)](n) (2) and [ZnCl2(4,7-phen)(2)] (3), where qz is quinazoline, 1,5-naph is 1,5-naphthyridine and 4,7-phen is 4,7-phenanthroline, were synthesized by the reactions of ZnCl2 and the corresponding N-heterocyclic ligand in 1:2 molar ratio in ethanol at ambient temperature. The characterization of these complexes was done by NMR, IR and UV-Vis spectroscopy, and their crystal structures were determined by single-crystal X-ray diffraction analysis. Complexes 1 and 3 are mononuclear species, in which Zn(II) ion is tetrahedrally coordinated by two nitrogen atoms belonging to two qz or 4,7-phen ligands, respectively, and by two chloride anions, while complex 2 is a 1D coordination polymer that contains 1,5-naph as bridging ligand between two metal ions. In agar disc-diffusion assay, complexes 1-3 manifested good inhibitory activity against two investigated Candida strains (C. albicans and C. parapsilosis), while not inducing toxic effects on the healthy human fibroblast cell line (MRC-5). This activity was not fungicidal, as revealed by the broth microdilution assay, however complex 3 showed the ability to modulate Candida hyphae formation, which is an important process during infection and showed significant synergistic effect with clinically used antifungal polyene nystatin.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Zinc(II) complexes with aromatic nitrogen-containing heterocycles as antifungal agents: Synergistic activity with clinically used drug nystatin
VL  - 208
DO  - 10.1016/j.jinorgbio.2020.111089
ER  - 

@article{
author = "Andrejević, Tina P. and Warzajtis, Beata and Glišić, Biljana and Vojnović, Sandra and Mojicević, Marija and Stevanović, Nevena Lj. and Nikodinović-Runić, Jasmina and Rychlewska, Urszula and Djuran, Milos",
year = "2020",
abstract = "Three novel Zn(II) complexes, [ZnCl2(qz)(2)] (1), [ZnCl2(1,5-naph)](n) (2) and [ZnCl2(4,7-phen)(2)] (3), where qz is quinazoline, 1,5-naph is 1,5-naphthyridine and 4,7-phen is 4,7-phenanthroline, were synthesized by the reactions of ZnCl2 and the corresponding N-heterocyclic ligand in 1:2 molar ratio in ethanol at ambient temperature. The characterization of these complexes was done by NMR, IR and UV-Vis spectroscopy, and their crystal structures were determined by single-crystal X-ray diffraction analysis. Complexes 1 and 3 are mononuclear species, in which Zn(II) ion is tetrahedrally coordinated by two nitrogen atoms belonging to two qz or 4,7-phen ligands, respectively, and by two chloride anions, while complex 2 is a 1D coordination polymer that contains 1,5-naph as bridging ligand between two metal ions. In agar disc-diffusion assay, complexes 1-3 manifested good inhibitory activity against two investigated Candida strains (C. albicans and C. parapsilosis), while not inducing toxic effects on the healthy human fibroblast cell line (MRC-5). This activity was not fungicidal, as revealed by the broth microdilution assay, however complex 3 showed the ability to modulate Candida hyphae formation, which is an important process during infection and showed significant synergistic effect with clinically used antifungal polyene nystatin.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Zinc(II) complexes with aromatic nitrogen-containing heterocycles as antifungal agents: Synergistic activity with clinically used drug nystatin",
volume = "208",
doi = "10.1016/j.jinorgbio.2020.111089"
}

Andrejević, T. P., Warzajtis, B., Glišić, B., Vojnović, S., Mojicević, M., Stevanović, N. Lj., Nikodinović-Runić, J., Rychlewska, U.,& Djuran, M.. (2020). Zinc(II) complexes with aromatic nitrogen-containing heterocycles as antifungal agents: Synergistic activity with clinically used drug nystatin. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 208.
https://doi.org/10.1016/j.jinorgbio.2020.111089

Andrejević TP, Warzajtis B, Glišić B, Vojnović S, Mojicević M, Stevanović NL, Nikodinović-Runić J, Rychlewska U, Djuran M. Zinc(II) complexes with aromatic nitrogen-containing heterocycles as antifungal agents: Synergistic activity with clinically used drug nystatin. in Journal of Inorganic Biochemistry. 2020;208.
doi:10.1016/j.jinorgbio.2020.111089 .

Andrejević, Tina P., Warzajtis, Beata, Glišić, Biljana, Vojnović, Sandra, Mojicević, Marija, Stevanović, Nevena Lj., Nikodinović-Runić, Jasmina, Rychlewska, Urszula, Djuran, Milos, "Zinc(II) complexes with aromatic nitrogen-containing heterocycles as antifungal agents: Synergistic activity with clinically used drug nystatin" in Journal of Inorganic Biochemistry, 208 (2020),
https://doi.org/10.1016/j.jinorgbio.2020.111089 . .

TY  - JOUR
AU  - Durić, Sonja Z.
AU  - Mojicević, Marija
AU  - Vojnović, Sandra
AU  - Wadepohl, Hubert
AU  - Andrejević, Tina P.
AU  - Stevanović, Nevena Lj.
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1369
AB  - In a continuing search for a novel metal-containing antimicrobial agents, the present study reports the synthesis, characterization and biological evaluation of two silver(I) complexes with 1,10-phenanthroline-based ligands, [Ag(1,10-phen)(2)]CF3COO center dot H2O (Ag1) and [Ag(CF3COO)(5,6-epoxy-1,10-phen)](2)(Ag2), 1,10-phen is 1, 10-phenanthroline and 5,6-epoxy-1,10-phen is 5,6-epoxy-5,6-dihydro-1,10-phenanthroline. The complexes were characterized by different spectroscopic techniques (IR, H-1 and C-13 NMR and UV-Vis), while the crystal structure of Ag2 complex was determined by a single-crystal X-ray diffraction analysis. The spectroscopic data confirmed that the structure of Ag1 complex, with silver(I) ion tetrahedrally coordinated by two bidentate 1, 10-phen ligands, is in accordance to the previous report (S.E. Paramonov et al., 2003). The crystallographic results showed that in dinuclear Ag2 complex, both Ag(I) ions are coordinated bidentately by 5,6-epoxy-1, 10-phen and monodentately by trifluoroacetate, with presence of the short Ag center dot center dot center dot Ag contact of 2.963 angstrom. Both silver (I) complexes were evaluated in vitro for antimicrobial activity against four bacterial and four Candida species, showing selectivity towards the investigated species of Candida with minimal inhibitory concentrations (MICs) between 0.9 and 12.5 mu M. Moreover, Ag2 complex manifested significant antiproliferative properties in the case of a range of human cell lines, including human breast cancer (MDA-MB 231), which resulted from the presence of epoxy functional group in the ligand. The gel electrophoresis results obtained from the studies of Ag1 and Ag2 interactions with bacteriophage lambda DNA (XDNA) suggested that these complexes did not cause DNA degradation.
PB  - Elsevier Science Sa, Lausanne
T2  - Inorganica Chimica Acta
T1  - Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity
VL  - 502
DO  - 10.1016/j.ica.2019.119357
ER  - 

@article{
author = "Durić, Sonja Z. and Mojicević, Marija and Vojnović, Sandra and Wadepohl, Hubert and Andrejević, Tina P. and Stevanović, Nevena Lj. and Nikodinović-Runić, Jasmina and Djuran, Milos and Glišić, Biljana",
year = "2020",
abstract = "In a continuing search for a novel metal-containing antimicrobial agents, the present study reports the synthesis, characterization and biological evaluation of two silver(I) complexes with 1,10-phenanthroline-based ligands, [Ag(1,10-phen)(2)]CF3COO center dot H2O (Ag1) and [Ag(CF3COO)(5,6-epoxy-1,10-phen)](2)(Ag2), 1,10-phen is 1, 10-phenanthroline and 5,6-epoxy-1,10-phen is 5,6-epoxy-5,6-dihydro-1,10-phenanthroline. The complexes were characterized by different spectroscopic techniques (IR, H-1 and C-13 NMR and UV-Vis), while the crystal structure of Ag2 complex was determined by a single-crystal X-ray diffraction analysis. The spectroscopic data confirmed that the structure of Ag1 complex, with silver(I) ion tetrahedrally coordinated by two bidentate 1, 10-phen ligands, is in accordance to the previous report (S.E. Paramonov et al., 2003). The crystallographic results showed that in dinuclear Ag2 complex, both Ag(I) ions are coordinated bidentately by 5,6-epoxy-1, 10-phen and monodentately by trifluoroacetate, with presence of the short Ag center dot center dot center dot Ag contact of 2.963 angstrom. Both silver (I) complexes were evaluated in vitro for antimicrobial activity against four bacterial and four Candida species, showing selectivity towards the investigated species of Candida with minimal inhibitory concentrations (MICs) between 0.9 and 12.5 mu M. Moreover, Ag2 complex manifested significant antiproliferative properties in the case of a range of human cell lines, including human breast cancer (MDA-MB 231), which resulted from the presence of epoxy functional group in the ligand. The gel electrophoresis results obtained from the studies of Ag1 and Ag2 interactions with bacteriophage lambda DNA (XDNA) suggested that these complexes did not cause DNA degradation.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Inorganica Chimica Acta",
title = "Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity",
volume = "502",
doi = "10.1016/j.ica.2019.119357"
}

Durić, S. Z., Mojicević, M., Vojnović, S., Wadepohl, H., Andrejević, T. P., Stevanović, N. Lj., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity. in Inorganica Chimica Acta
Elsevier Science Sa, Lausanne., 502.
https://doi.org/10.1016/j.ica.2019.119357

Durić SZ, Mojicević M, Vojnović S, Wadepohl H, Andrejević TP, Stevanović NL, Nikodinović-Runić J, Djuran M, Glišić B. Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity. in Inorganica Chimica Acta. 2020;502.
doi:10.1016/j.ica.2019.119357 .

Durić, Sonja Z., Mojicević, Marija, Vojnović, Sandra, Wadepohl, Hubert, Andrejević, Tina P., Stevanović, Nevena Lj., Nikodinović-Runić, Jasmina, Djuran, Milos, Glišić, Biljana, "Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity" in Inorganica Chimica Acta, 502 (2020),
https://doi.org/10.1016/j.ica.2019.119357 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Vojnović, Sandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1212
AB  - The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.
PB  - Springer, New York
T2  - International Microbiology
T1  - Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)
EP  - 353
IS  - 3
SP  - 343
VL  - 22
DO  - 10.1007/s10123-019-00054-8
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Nikodinović-Runić, Jasmina and Vasiljević, Branka and Gulder, Tobias A. M. and Vojnović, Sandra",
year = "2019",
abstract = "The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.",
publisher = "Springer, New York",
journal = "International Microbiology",
title = "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)",
pages = "353-343",
number = "3",
volume = "22",
doi = "10.1007/s10123-019-00054-8"
}

Mojicević, M., D'Agostino, P. M., Nikodinović-Runić, J., Vasiljević, B., Gulder, T. A. M.,& Vojnović, S.. (2019). Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology
Springer, New York., 22(3), 343-353.
https://doi.org/10.1007/s10123-019-00054-8

Mojicević M, D'Agostino PM, Nikodinović-Runić J, Vasiljević B, Gulder TAM, Vojnović S. Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology. 2019;22(3):343-353.
doi:10.1007/s10123-019-00054-8 .

Mojicević, Marija, D'Agostino, Paul M., Nikodinović-Runić, Jasmina, Vasiljević, Branka, Gulder, Tobias A. M., Vojnović, Sandra, "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)" in International Microbiology, 22, no. 3 (2019):343-353,
https://doi.org/10.1007/s10123-019-00054-8 . .

TY  - JOUR
AU  - Ajdačić, Vladimir
AU  - Lazić, Jelena
AU  - Mojicević, Marija
AU  - Segan, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Opsenica, Igor M.
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1010
AB  - A series of novel guanylhydrazones were designed, synthesized and characterized. All the compounds were screened for their antibacterial and antifungal activity. Compounds 26 and 27 showed excellent antibacterial activities against Staphylococcus aureus ATCC 25923 and Micrococcus luteus ATCC 379 with minimal inhibitory concentrations of 4 ae g mL(-1), and good antifungal activity against Candida parapsilosis ATCC 22019. These results suggested that the selected guanylhydrazones could serve as promising leads for improved antimicrobial development.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Antibacterial and antifungal properties of guanylhydrazones
EP  - 649
IS  - 6
SP  - 641
VL  - 82
DO  - 10.2298/JSC170213033A
ER  - 

@article{
author = "Ajdačić, Vladimir and Lazić, Jelena and Mojicević, Marija and Segan, Sandra and Nikodinović-Runić, Jasmina and Opsenica, Igor M.",
year = "2017",
abstract = "A series of novel guanylhydrazones were designed, synthesized and characterized. All the compounds were screened for their antibacterial and antifungal activity. Compounds 26 and 27 showed excellent antibacterial activities against Staphylococcus aureus ATCC 25923 and Micrococcus luteus ATCC 379 with minimal inhibitory concentrations of 4 ae g mL(-1), and good antifungal activity against Candida parapsilosis ATCC 22019. These results suggested that the selected guanylhydrazones could serve as promising leads for improved antimicrobial development.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Antibacterial and antifungal properties of guanylhydrazones",
pages = "649-641",
number = "6",
volume = "82",
doi = "10.2298/JSC170213033A"
}

Ajdačić, V., Lazić, J., Mojicević, M., Segan, S., Nikodinović-Runić, J.,& Opsenica, I. M.. (2017). Antibacterial and antifungal properties of guanylhydrazones. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(6), 641-649.
https://doi.org/10.2298/JSC170213033A

Ajdačić V, Lazić J, Mojicević M, Segan S, Nikodinović-Runić J, Opsenica IM. Antibacterial and antifungal properties of guanylhydrazones. in Journal of the Serbian Chemical Society. 2017;82(6):641-649.
doi:10.2298/JSC170213033A .

Ajdačić, Vladimir, Lazić, Jelena, Mojicević, Marija, Segan, Sandra, Nikodinović-Runić, Jasmina, Opsenica, Igor M., "Antibacterial and antifungal properties of guanylhydrazones" in Journal of the Serbian Chemical Society, 82, no. 6 (2017):641-649,
https://doi.org/10.2298/JSC170213033A . .