TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1395
AB  - Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - Journal of Clinical Gastroenterology
T1  - The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11
EP  - S12
SP  - S11
VL  - 54
DO  - 10.1097/MCG.0000000000001292
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1395
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2020",
abstract = "Objective Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment of diabetic rats showed decreased hyperglycemia and ameliorating effect on diabetes-associated damage of liver and kidneys. Hence, the aim of this study was to reveal the effects of BGCG11 probiotic on gut microbiota composition and monitoring the insulin production in pancreatic islets in diabetic rats. Methods Experiments were performed on albino Wistar rats divided into four groups: ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes; P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were orally administered with BGCG11, one week before (P/D/P) and after the STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from all fecal samples and rDNA amplicons were analyzed by DGGE and 16S rDNA genes sequencing. For immunohistochemical analysis, slides were stained with anti-insulin antibody and secondary antibody coupled with horseradish peroxidase. Results The results revealed the higher diversity of gut microbiota in D/P group comparing to D group, as well as the higher prevalence of Flintibacter butyricus (the major butyric producer), Acetatifactor muris (present in obese mouse) and Eisenbergiella massiliensis (found in obese woman), while the lipolytic bacterium Aestuariispira insulae was more prevalent in diabetic rats. In both, P/D/P and D/P group, increased number of positive immunoreactions of β-cells for anti-insulin antibodies was displayed in compare to D group with islet atrophy. Conclusions The results of this study suggest that the positive effect of BGCG11 on STZ-induced diabetes in rats could be annotated to its protective role on the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "Journal of Clinical Gastroenterology",
title = "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11",
pages = "S12-S11",
volume = "54",
doi = "10.1097/MCG.0000000000001292",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1395"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2020). The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology
Wolters Kluwer Health., 54, S11-S12.
https://doi.org/10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11. in Journal of Clinical Gastroenterology. 2020;54:S11-S12.
doi:10.1097/MCG.0000000000001292
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The Analysis of Fecal Microbiota and Insulin Production in Diabetic Rats After Oral Administration of Probiotic Lactobacillus Paraplantarum BGCG11" in Journal of Clinical Gastroenterology, 54 (2020):S11-S12,
https://doi.org/10.1097/MCG.0000000000001292 .,
https://hdl.handle.net/21.15107/rcub_imagine_1395 .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Živković, Milica
AU  - Arambasić-Jovanović, Jelena
AU  - Tolinački, Maja
AU  - Sinadinović, Marija
AU  - Rajić, Jovana
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Golić, Nataša
AU  - Vidaković, Melita
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1039
AB  - The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBP beta. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in alpha-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats
EP  - 437
SP  - 427
VL  - 38
DO  - 10.1016/j.jff.2017.09.033
ER  - 

@article{
author = "Mihailović, Mirjana and Živković, Milica and Arambasić-Jovanović, Jelena and Tolinački, Maja and Sinadinović, Marija and Rajić, Jovana and Uskoković, Aleksandra and Dinić, Svetlana and Grdović, Nevena and Golić, Nataša and Vidaković, Melita",
year = "2017",
abstract = "The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBP beta. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in alpha-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats",
pages = "437-427",
volume = "38",
doi = "10.1016/j.jff.2017.09.033"
}

Mihailović, M., Živković, M., Arambasić-Jovanović, J., Tolinački, M., Sinadinović, M., Rajić, J., Uskoković, A., Dinić, S., Grdović, N., Golić, N.,& Vidaković, M.. (2017). Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 38, 427-437.
https://doi.org/10.1016/j.jff.2017.09.033

Mihailović M, Živković M, Arambasić-Jovanović J, Tolinački M, Sinadinović M, Rajić J, Uskoković A, Dinić S, Grdović N, Golić N, Vidaković M. Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods. 2017;38:427-437.
doi:10.1016/j.jff.2017.09.033 .

Mihailović, Mirjana, Živković, Milica, Arambasić-Jovanović, Jelena, Tolinački, Maja, Sinadinović, Marija, Rajić, Jovana, Uskoković, Aleksandra, Dinić, Svetlana, Grdović, Nevena, Golić, Nataša, Vidaković, Melita, "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats" in Journal of Functional Foods, 38 (2017):427-437,
https://doi.org/10.1016/j.jff.2017.09.033 . .