TY  - JOUR
AU  - Stanković, Srdan
AU  - Vasiljević, Branka
AU  - Jeremić, Sanja
AU  - Cvetković, Vladica
AU  - Morić, Ivana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1084
AB  - Extremely acidic environments are frequently formed in areas impacted by mining activities, and Lake Robule is such an ecosystem. Although an extreme environment, Lake Robule is inhabited by acidophilic microorganisms. We investigated biodiversity of the macroscopic structure known as a microbial mat formed on the lake bottom in shallow waters. Microbial mats are common in acidic environments, but their composition can differ significantly from site to site. Microbial diversity of the mat from Lake Robule was investigated using both cultivation-dependent and metagenomic approaches. The results showed the mat to be mostly inhabited by heterotrophic acidophilic bacteria. When compared to the microbial community of Lake Robule's surface water, the microbial mat proved to be a more complex community. A biogeochemical model of the mat of Lake Robule is proposed on the basis of our results and available published data.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)
EP  - 54
IS  - 1
SP  - 47
VL  - 41
DO  - 10.5281/zenodo.453792
ER  - 

@article{
author = "Stanković, Srdan and Vasiljević, Branka and Jeremić, Sanja and Cvetković, Vladica and Morić, Ivana",
year = "2017",
abstract = "Extremely acidic environments are frequently formed in areas impacted by mining activities, and Lake Robule is such an ecosystem. Although an extreme environment, Lake Robule is inhabited by acidophilic microorganisms. We investigated biodiversity of the macroscopic structure known as a microbial mat formed on the lake bottom in shallow waters. Microbial mats are common in acidic environments, but their composition can differ significantly from site to site. Microbial diversity of the mat from Lake Robule was investigated using both cultivation-dependent and metagenomic approaches. The results showed the mat to be mostly inhabited by heterotrophic acidophilic bacteria. When compared to the microbial community of Lake Robule's surface water, the microbial mat proved to be a more complex community. A biogeochemical model of the mat of Lake Robule is proposed on the basis of our results and available published data.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)",
pages = "54-47",
number = "1",
volume = "41",
doi = "10.5281/zenodo.453792"
}

Stanković, S., Vasiljević, B., Jeremić, S., Cvetković, V.,& Morić, I.. (2017). Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 41(1), 47-54.
https://doi.org/10.5281/zenodo.453792

Stanković S, Vasiljević B, Jeremić S, Cvetković V, Morić I. Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia). in Botanica Serbica. 2017;41(1):47-54.
doi:10.5281/zenodo.453792 .

Stanković, Srdan, Vasiljević, Branka, Jeremić, Sanja, Cvetković, Vladica, Morić, Ivana, "Evaluation of microbial diversity of the microbial mat from the extremely acidic Lake Robule (Bor, Serbia)" in Botanica Serbica, 41, no. 1 (2017):47-54,
https://doi.org/10.5281/zenodo.453792 . .

TY  - JOUR
AU  - Stanković, Srdan
AU  - Morić, Ivana
AU  - Pavić, Aleksandar
AU  - Vasiljević, Branka
AU  - Johnson, D. Barrie
AU  - Cvetković, Vladica
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/712
AB  - An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)
EP  - 741
IS  - 6
SP  - 729
VL  - 79
DO  - 10.2298/JSC130605071S
ER  - 

@article{
author = "Stanković, Srdan and Morić, Ivana and Pavić, Aleksandar and Vasiljević, Branka and Johnson, D. Barrie and Cvetković, Vladica",
year = "2014",
abstract = "An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)",
pages = "741-729",
number = "6",
volume = "79",
doi = "10.2298/JSC130605071S"
}

Stanković, S., Morić, I., Pavić, A., Vasiljević, B., Johnson, D. B.,& Cvetković, V.. (2014). Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 79(6), 729-741.
https://doi.org/10.2298/JSC130605071S

Stanković S, Morić I, Pavić A, Vasiljević B, Johnson DB, Cvetković V. Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society. 2014;79(6):729-741.
doi:10.2298/JSC130605071S .

Stanković, Srdan, Morić, Ivana, Pavić, Aleksandar, Vasiljević, Branka, Johnson, D. Barrie, Cvetković, Vladica, "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)" in Journal of the Serbian Chemical Society, 79, no. 6 (2014):729-741,
https://doi.org/10.2298/JSC130605071S . .