TY  - CONF
AU  - Galić, Ivana
AU  - Bez, Christina
AU  - Bertani, Iris
AU  - Simić, Milena
AU  - Venturi, Vittorio
AU  - Stanković, Nada
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2370
AB  - The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2370
ER  - 

@conference{
author = "Galić, Ivana and Bez, Christina and Bertani, Iris and Simić, Milena and Venturi, Vittorio and Stanković, Nada",
year = "2024",
abstract = "The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2370"
}

Galić, I., Bez, C., Bertani, I., Simić, M., Venturi, V.,& Stanković, N.. (2024). SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370

Galić I, Bez C, Bertani I, Simić M, Venturi V, Stanković N. SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

Galić, Ivana, Bez, Christina, Bertani, Iris, Simić, Milena, Venturi, Vittorio, Stanković, Nada, "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2159
AB  - Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.
PB  - Belgrade : Faculty of Biology
C3  - ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
T1  - Herbicide-resilient bacterial consortia in plant growth promotion
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2159
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "Modern agriculture relies heavily on agrochemicals that can adversely affect
non-target organisms, including microorganisms. In terms of agricultural sustainability,
herbicideresilient plant growth-promoting (PGP) bacteria that can
improve crop yield are critical. Previously, we isolated 122 high herbicide concentration
tolerant (HHCT) bacteria from soils contaminated with S-metolachlor,
a widely used herbicide. Initially, in vitro experiments were conducted to screen
potential candidates for further evaluation. Our goal was to identify HHCT bacteria
with high PGP potential, and the ability to improve soil quality and biocontrol
activity. Subsequently, the most promising candidates were grouped
into three consortia and evaluated in pot for their effects on corn seed germination,
early-stage plant development, and root colonization. Experiments were
conducted with seeds of commercial maize hybrids under controlled conditions,
with and without the presence of the herbicide S-metolachlor. The results
showed that the consortia positively affected seed germination and promoted
higher germination rates. The bacterial isolates showed the ability to colonize
the root system of maize, with significant effects of the consortia on shoot and
root biomass and shoot and root length. However, it was observed in some
consortia that the presence of herbicides resulted in plant damage. Markedly,
the presence of the herbicide S-metolachlor reduced or reversed the beneficial
effects of bacterial consortia. This could be due to incomplete biotransformation
of the herbicide leading to the formation of toxic intermediates. The selection
of bacterial consortia that not only promote plant growth but also have the ability
to fully biotransform herbicides is critical. In conclusion, the isolated HHCT
bacteria showed promising PGP potential and formed an effective consortium
to promote maize seed germination and early-stage development. However,
careful consideration of the selection of bacterial consortia is required to ensure
their compatibility with herbicides and to minimize negative impacts on plant
health and environmental sustainability.",
publisher = "Belgrade : Faculty of Biology",
journal = "ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture",
title = "Herbicide-resilient bacterial consortia in plant growth promotion",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2159"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture
Belgrade : Faculty of Biology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159

Galić I, Bertani I, Bez C, Stanković N, Venturi V. Herbicide-resilient bacterial consortia in plant growth promotion. in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "Herbicide-resilient bacterial consortia in plant growth promotion" in ICGEB WORKSHOP: Trends in microbial solutions for sustainable agriculture (2023):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2159 .

TY  - CONF
AU  - Bez, Cristina
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2012
AB  - In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding
EP  - 71
SP  - 71
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2012
ER  - 

@conference{
author = "Bez, Cristina and Galić, Ivana and Bertani, Iris and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "In terms of agricultural sustainability, herbicide-resistant, plant growth promoting (PGP)
bacteria that can improve crop yield are valuable resource. To exhibit PGP traits, the
bacteria must be able to colonize and survive in the rhizosphere.
Upon screening the herbicide-resistant bacterial collection, candidates with the highest
PGP potential were grouped into three consortia to evaluate their ability to colonize roots
and persist in the natural/local plant microbiome in the pot. Experiments were conducted
with seeds of commercial maize hybrids under controlled conditions, with and without
herbicide. Colonization ability was evaluated by examining multiple plants from each
treatment at two-time points during the experiment. 16S rRNA amplicon community
profiling was performed to precisely target the bacterial strains used in the three
consortia and investigate how the local microbiome might be altered by the application of
the consortia. Bioinformatic analysis was performed using qiime2, clustering of reads into
amplicon sequence variants ASVs using the DADA2 plugin, and the taxonomic assignment
was based on a customized dataset formed from the 16S rRNA gene sequences of the
ten isolates used in this study or by using the Silva rRNA database. For clustering and
comparison of ASVs based on sequence similarity, the program cd-hit was used, with
the sequence similarity parameter set to 98% to be considered part of the same cluster.
The obtained dataset was imported into R using the package qiime2R, and subsequent
analyzes and graphs were generated using either the R packages phyloseq, microbiome,
or reshape2. We identified seven out of ten inoculated strains in both time points tested
and with comparable abundance, indicating that most of the bacterial isolates tested have
the ability to colonize the root system of maize. Furthermore, the natural/local microbiome
of maize plants is not disturbed by the three consortia used in this study, implying that
they are good candidates for future biotechnological applications.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding",
pages = "71-71",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2012"
}

Bez, C., Galić, I., Bertani, I., Stanković, N.,& Venturi, V.. (2023). Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012

Bez C, Galić I, Bertani I, Stanković N, Venturi V. Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding. in 4th Belgrade Bioinformatics Conference. 2023;4:71-71.
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

Bez, Cristina, Galić, Ivana, Bertani, Iris, Stanković, Nada, Venturi, Vittorio, "Root colonization ability of herbicide-resistant PGP bacteria evaluated by 16S rRNA metabarcoding" in 4th Belgrade Bioinformatics Conference, 4 (2023):71-71,
https://hdl.handle.net/21.15107/rcub_imagine_2012 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bertani, Iris
AU  - Bez, Cristina
AU  - Stanković, Nada
AU  - Venturi, Vittorio
PY  - 2023
UR  - https://ceesme.ecolres.hu/CEESME2023_abstract_book_v1.pdf
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2163
AB  - organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.
C3  - CEESME Central and Eastern Europe Symposium on Microbial Ecology
T1  - High herbicide concentration tolerant bacteria in plant growth promotion
EP  - 98
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2163
ER  - 

@conference{
author = "Galić, Ivana and Bertani, Iris and Bez, Cristina and Stanković, Nada and Venturi, Vittorio",
year = "2023",
abstract = "organisms, including microorganisms. In the global effort to increase agriculture sustainability, there
is an emerging need for herbicide-resilient plant growth-promoting (PGP) bacteria that improve
essential crops yield. From S-metolachlor-contaminated soils, we isolated 122 strains of high herbicide
concentration tolerant (HHCT) bacteria capable of growing on or using herbicide as their sole carbon
source. The aim of our study was to identify HHCT bacteria with the highest PGP potential, soil quality
improvement ability, biocontrol activity, and plant colonization capability; then combine them into
consortia that include all these traits, and test them in planta. PGP property evaluation included assays
for nitrogen fixation, phosphate solubilization, IAA, siderophore, and exopolysaccharide production,
detection of extracellular enzymatic activities, and motility. Strains were identified through 16S rDNA
sequencing. Biocontrol potential was evaluated against plant pathogens such as Fusarium sp. , and P.
fuscovaginae. The most promising candidates were combined in three consortia for growth-chamber
experiments to investigate the promotion of maize seed germination, early-stage plant development,
and root colonization evaluated by metagenomic analysis. The HHCT bacteria showed promising
PGP potential. 32% of the collection exhibited 3-4 of four tested direct PGP mechanisms. 22 isolates
had antifungal, and 13 had antibacterial activity. The strains with the highest in vitro PGP activity
predominantly belonged to Serratia, Pseudomonas, and Sphingobacterium genera. These strains
formed the most effective consortium that promoted seed germination and increased shoot and root
dry weight at the early-stage development.",
journal = "CEESME Central and Eastern Europe Symposium on Microbial Ecology",
title = "High herbicide concentration tolerant bacteria in plant growth promotion",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2163"
}

Galić, I., Bertani, I., Bez, C., Stanković, N.,& Venturi, V.. (2023). High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology, 98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163

Galić I, Bertani I, Bez C, Stanković N, Venturi V. High herbicide concentration tolerant bacteria in plant growth promotion. in CEESME Central and Eastern Europe Symposium on Microbial Ecology. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2163 .

Galić, Ivana, Bertani, Iris, Bez, Cristina, Stanković, Nada, Venturi, Vittorio, "High herbicide concentration tolerant bacteria in plant growth promotion" in CEESME Central and Eastern Europe Symposium on Microbial Ecology (2023):98-98,
https://hdl.handle.net/21.15107/rcub_imagine_2163 .

TY  - JOUR
AU  - Đokić, Lidija
AU  - Stanković, Nada
AU  - Galić, Ivana
AU  - Morić, Ivana
AU  - Radaković, Nataša
AU  - Segan, Sandra
AU  - Pavić, Aleksandar
AU  - Šenerović, Lidija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1596
AB  - Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo
VL  - 13
DO  - 10.3389/fmicb.2022.906312
ER  - 

@article{
author = "Đokić, Lidija and Stanković, Nada and Galić, Ivana and Morić, Ivana and Radaković, Nataša and Segan, Sandra and Pavić, Aleksandar and Šenerović, Lidija",
year = "2022",
abstract = "Bacterial infections have become increasingly difficult to treat due to the occurrence of antibiotic-resistant strains. A promising strategy to increase the efficacy of therapy is to combine antibacterials with agents that decrease pathogen virulence via the modulation of the quorum sensing (QS). Lactonases inhibit acylated homoserine lactone (AHL)-mediated QS in Gram-negative bacteria, including the leading nosocomial pathogen Pseudomonas aeruginosa. Here we describe the characteristics of heterologously expressed YtnP lactonase from Bacillus paralicheniformis ZP1 (YtnP-ZP1) isolated from agricultural soil using the culture enrichment method. Purified YtnP-ZP1 hydrolyzed different AHLs with preference to substrates with long acyl residues as evaluated in assays with biosensors and HPLC. The enzyme showed good thermostability and activity in a wide temperature range. YtnP-ZP1 in 50 mu g mL(-1) concentration reduced the amount of P. aeruginosa-produced long-chain AHLs by 85%, while it hydrolyzed 50% of short-chain AHLs. Incubation of P. aeruginosa PAO1 with YtnP-ZP1 reduced its swarming motility and elastolytic activity without bactericidal effect. YtnP-ZP1 caused the inhibition of biofilm formation and disintegration of mature biofilms in P. aeruginosa PAO1 and multiresistant clinical strain BR5H that was visualized by crystal violet staining. The treatment with YtnP-ZP1 in concentrations higher than 25 mu g mL(-1) improved the survival of P. aeruginosa PAO1-infected zebrafish (Danio rerio), rescuing 80% of embryos, while in combination with tobramycin or gentamicin survival rate increased to 100%. The treatment of P. aeruginosa PAO1 biofilms on infected zebrafish tail wounds with 50 mu g mL(-1) YtnP-ZP1 and 2 x MIC tobramycin led to infection clearing in 2 days. The extensive toxicity studies proved YtnP-ZP1 was non-toxic to human cells and zebrafish. In conclusion, novel YtnP-ZP1 lactonase with its effective anti-virulence activity could be used to increase the efficacy of clinically approved antibiotics in clearing both systemic and biofilm-associated P. aeruginosa infections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo",
volume = "13",
doi = "10.3389/fmicb.2022.906312"
}

Đokić, L., Stanković, N., Galić, I., Morić, I., Radaković, N., Segan, S., Pavić, A.,& Šenerović, L.. (2022). Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fmicb.2022.906312

Đokić L, Stanković N, Galić I, Morić I, Radaković N, Segan S, Pavić A, Šenerović L. Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo. in Frontiers in Microbiology. 2022;13.
doi:10.3389/fmicb.2022.906312 .

Đokić, Lidija, Stanković, Nada, Galić, Ivana, Morić, Ivana, Radaković, Nataša, Segan, Sandra, Pavić, Aleksandar, Šenerović, Lidija, "Novel Quorum Quenching YtnP Lactonase From Bacillus paralicheniformis Reduces Pseudomonas aeruginosa Virulence and Increases Antibiotic Efficacy in vivo" in Frontiers in Microbiology, 13 (2022),
https://doi.org/10.3389/fmicb.2022.906312 . .

TY  - CONF
AU  - Galić, Ivana
AU  - Šenerović, Lidija
AU  - Stanković, Nada
PY  - 2022
UR  - https://2022.eccmid.org/abstracts/posters
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2162
AB  - Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.
C3  - European Congress of Clinical Microbiology & Infectious Diseases
T1  - Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2162
ER  - 

@conference{
author = "Galić, Ivana and Šenerović, Lidija and Stanković, Nada",
year = "2022",
abstract = "Soils contain microorganisms capable of causing diseases in humans, either
as opportunistic or as obligate pathogens. The application of herbicides
increases the prevalence of antibiotic resistance genes and mobile genetic
elements, which expands the competitive niche of pathogenic microbes. This
implies that herbicides could impose selective pressure driving the spread of
antibiotic resistance and therefore making heavily herbicide treated agricultural
soil a possible reservoir of multiresistant opportunistic human pathogens.
From the soil exposed to chlorinated aromatic herbicides S-metolachlor
and isoxaflutole, semi-persistent selective herbicides widely used in
European Union and Serbia we isolated bacteria able to grow on herbicide as
the sole carbon source. Bacterial isolates were assessed for various virulence
traits and resistance to antibiotics in order to identify the strains with high
pathogenic potential.",
journal = "European Congress of Clinical Microbiology & Infectious Diseases",
title = "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2162"
}

Galić, I., Šenerović, L.,& Stanković, N.. (2022). Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases, 32.
https://hdl.handle.net/21.15107/rcub_imagine_2162

Galić I, Šenerović L, Stanković N. Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans. in European Congress of Clinical Microbiology & Infectious Diseases. 2022;32.
https://hdl.handle.net/21.15107/rcub_imagine_2162 .

Galić, Ivana, Šenerović, Lidija, Stanković, Nada, "Potential of environmental Serratia from herbicide treated soil for pathogenicity in humans" in European Congress of Clinical Microbiology & Infectious Diseases, 32 (2022),
https://hdl.handle.net/21.15107/rcub_imagine_2162 .