TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Vlajnić, Marina
AU  - Ristanović, Momcilo
AU  - Petrović, Jelena
AU  - Dimitrijević, Ivan
AU  - Krivokapić, Zoran
AU  - Radojković, Dragica
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1036
AB  - Purpose: To analyze if cell free (cf)DNA levels and the presence of KRAS and BRAF mutations in serum could be used as diagnostic biomarkers in patients with primary colorectal cancer (CRC). Methods: This study included 92 individuals who were operated due to primary CRC (N=52;study group) and to hemorrhoids (N=40;control group). Serum cfDNA levels were measured with real-time PCR (RT-PCR) using PicoGreen dsDNA quantitation reagent. Colorectal tissue and related blood and serum samples taken at the time of surgery were subjected to DNA extraction and analysis of KRAS and BRAF mutations based on multiplex SNaPshot assay and DNA sequencing. Results: The average cfDNA concentration was lower in patients of the study group (20 +/- 7 ng/mu L) in comparison to controls (34 +/- 9 ng/mu L) and this difference was statistically significant (p lt 0.001). The SNaPshot analysis detected KRAS c35 mutations in colorectal tumor tissue in 14 cases, but the presence of the mutation was not confirmed in cfD-NA extracted from blood samples of these patients. Conclusions: The level of serum cfDNA in CRC is decreased in comparison to patients with hemorrhoids, which questions the usefulness of cfDNA as cancer biomarker. Also, cfDNA does not appear to be suitable as a source for mutation detection in this disease.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of Buon
T1  - Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer
EP  - 183
IS  - 1
SP  - 178
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1036
ER  - 

@article{
author = "Nikolić, Aleksandra and Vlajnić, Marina and Ristanović, Momcilo and Petrović, Jelena and Dimitrijević, Ivan and Krivokapić, Zoran and Radojković, Dragica",
year = "2017",
abstract = "Purpose: To analyze if cell free (cf)DNA levels and the presence of KRAS and BRAF mutations in serum could be used as diagnostic biomarkers in patients with primary colorectal cancer (CRC). Methods: This study included 92 individuals who were operated due to primary CRC (N=52;study group) and to hemorrhoids (N=40;control group). Serum cfDNA levels were measured with real-time PCR (RT-PCR) using PicoGreen dsDNA quantitation reagent. Colorectal tissue and related blood and serum samples taken at the time of surgery were subjected to DNA extraction and analysis of KRAS and BRAF mutations based on multiplex SNaPshot assay and DNA sequencing. Results: The average cfDNA concentration was lower in patients of the study group (20 +/- 7 ng/mu L) in comparison to controls (34 +/- 9 ng/mu L) and this difference was statistically significant (p lt 0.001). The SNaPshot analysis detected KRAS c35 mutations in colorectal tumor tissue in 14 cases, but the presence of the mutation was not confirmed in cfD-NA extracted from blood samples of these patients. Conclusions: The level of serum cfDNA in CRC is decreased in comparison to patients with hemorrhoids, which questions the usefulness of cfDNA as cancer biomarker. Also, cfDNA does not appear to be suitable as a source for mutation detection in this disease.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of Buon",
title = "Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer",
pages = "183-178",
number = "1",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1036"
}

Nikolić, A., Vlajnić, M., Ristanović, M., Petrović, J., Dimitrijević, I., Krivokapić, Z.,& Radojković, D.. (2017). Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer. in Journal of Buon
Balkan Union of Oncology (B.U.ON.)., 22(1), 178-183.
https://hdl.handle.net/21.15107/rcub_imagine_1036

Nikolić A, Vlajnić M, Ristanović M, Petrović J, Dimitrijević I, Krivokapić Z, Radojković D. Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer. in Journal of Buon. 2017;22(1):178-183.
https://hdl.handle.net/21.15107/rcub_imagine_1036 .

Nikolić, Aleksandra, Vlajnić, Marina, Ristanović, Momcilo, Petrović, Jelena, Dimitrijević, Ivan, Krivokapić, Zoran, Radojković, Dragica, "Cell-free DNA as biomarker and source for mutation detection in primary colorectal cancer" in Journal of Buon, 22, no. 1 (2017):178-183,
https://hdl.handle.net/21.15107/rcub_imagine_1036 .