TY  - JOUR
AU  - Ljujić, Mila
AU  - Mijatović, S.
AU  - Bulatović, M. Z.
AU  - Mojić, M.
AU  - Maksimović-Ivanić, Danijela
AU  - Radojković, Dragica
AU  - Topić, Aleksandra
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/949
AB  - Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-kappa B. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Molecular Biology
T1  - ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)
EP  - 156
IS  - 1
SP  - 153
VL  - 50
DO  - 10.1134/S002689331601012X
ER  - 

@article{
author = "Ljujić, Mila and Mijatović, S. and Bulatović, M. Z. and Mojić, M. and Maksimović-Ivanić, Danijela and Radojković, Dragica and Topić, Aleksandra",
year = "2016",
abstract = "Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-kappa B. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Molecular Biology",
title = "ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)",
pages = "156-153",
number = "1",
volume = "50",
doi = "10.1134/S002689331601012X"
}

Ljujić, M., Mijatović, S., Bulatović, M. Z., Mojić, M., Maksimović-Ivanić, D., Radojković, D.,& Topić, A.. (2016). ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molecular Biology
Maik Nauka/Interperiodica/Springer, New York., 50(1), 153-156.
https://doi.org/10.1134/S002689331601012X

Ljujić M, Mijatović S, Bulatović MZ, Mojić M, Maksimović-Ivanić D, Radojković D, Topić A. ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molecular Biology. 2016;50(1):153-156.
doi:10.1134/S002689331601012X .

Ljujić, Mila, Mijatović, S., Bulatović, M. Z., Mojić, M., Maksimović-Ivanić, Danijela, Radojković, Dragica, Topić, Aleksandra, "ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)" in Molecular Biology, 50, no. 1 (2016):153-156,
https://doi.org/10.1134/S002689331601012X . .

TY  - JOUR
AU  - Radović, J.
AU  - Maksimović-Ivanić, Danijela
AU  - Timotijević, Gordana
AU  - Popadić, S.
AU  - Ramić, Z.
AU  - Trajković, V.
AU  - Miljković, D.
AU  - Stošić-Grujičić, Stanislava
AU  - Mijatović, S.
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/550
AB  - Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Cell-type dependent response of melanoma cells to aloe emodin
EP  - 3189
IS  - 9
SP  - 3181
VL  - 50
DO  - 10.1016/j.fct.2012.05.047
ER  - 

@article{
author = "Radović, J. and Maksimović-Ivanić, Danijela and Timotijević, Gordana and Popadić, S. and Ramić, Z. and Trajković, V. and Miljković, D. and Stošić-Grujičić, Stanislava and Mijatović, S.",
year = "2012",
abstract = "Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Cell-type dependent response of melanoma cells to aloe emodin",
pages = "3189-3181",
number = "9",
volume = "50",
doi = "10.1016/j.fct.2012.05.047"
}

Radović, J., Maksimović-Ivanić, D., Timotijević, G., Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, S.,& Mijatović, S.. (2012). Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 50(9), 3181-3189.
https://doi.org/10.1016/j.fct.2012.05.047

Radović J, Maksimović-Ivanić D, Timotijević G, Popadić S, Ramić Z, Trajković V, Miljković D, Stošić-Grujičić S, Mijatović S. Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology. 2012;50(9):3181-3189.
doi:10.1016/j.fct.2012.05.047 .

Radović, J., Maksimović-Ivanić, Danijela, Timotijević, Gordana, Popadić, S., Ramić, Z., Trajković, V., Miljković, D., Stošić-Grujičić, Stanislava, Mijatović, S., "Cell-type dependent response of melanoma cells to aloe emodin" in Food and Chemical Toxicology, 50, no. 9 (2012):3181-3189,
https://doi.org/10.1016/j.fct.2012.05.047 . .

TY  - CONF
AU  - Mijatović, S.
AU  - Radović, J.
AU  - Timotijević, Gordana
AU  - Mojić, M.
AU  - Miljković, D.
AU  - Dekanski, D.
AU  - Stošić-Grujičić, Stanislava
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/349
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway
EP  - 903
IS  - 9
SP  - 903
VL  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1443
ER  - 

@conference{
author = "Mijatović, S. and Radović, J. and Timotijević, Gordana and Mojić, M. and Miljković, D. and Dekanski, D. and Stošić-Grujičić, Stanislava",
year = "2009",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway",
pages = "903-903",
number = "9",
volume = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1443"
}

Mijatović, S., Radović, J., Timotijević, G., Mojić, M., Miljković, D., Dekanski, D.,& Stošić-Grujičić, S.. (2009). Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 75(9), 903-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443

Mijatović S, Radović J, Timotijević G, Mojić M, Miljković D, Dekanski D, Stošić-Grujičić S. Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica. 2009;75(9):903-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .

Mijatović, S., Radović, J., Timotijević, Gordana, Mojić, M., Miljković, D., Dekanski, D., Stošić-Grujičić, Stanislava, "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway" in Planta Medica, 75, no. 9 (2009):903-903,
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .